RBC Lysis Buffer (10X) from BioLegend

Product Specs
ItemRBC Lysis Buffer (10X)
CompanyBioLegend
Price $65.00Supplier Page View Company Product Page
Catalog Number420301
Quantity100ML
FormatThis red blood cell (RBC) lysis buffer is supplied as a 10X solution and should be diluted to 1X in deionized water. 100 ml of 10X concentrate will yield a quantity of 1X solution that is sufficient to lyse 500 samples
ApplicationsFC

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Underlying MASLD-induced gut microbiome dysbiosis and intestinal inflammation are key to poor outcomes in vibriosis infections in a preclinical modelGut MicrobesDecember 31, 2026Punnag Saha, Subhajit Roy, Madhura More, Dipro Bose, Ayushi Trivedi, Bryan W. Brooks, Wing-Kin Syn, Anna Mae Diehl, Saurabh Chatterjeeg for 7 min at 4 °C to pellet down the NPCs, and 1x red blood cell (RBC) lysis buffer (Catalog No. 420301, BioLegend, San Diego, CA, USA) was added to remove any traces of erythrocytes. Finally, the isolated

(2) DNA prime and peptide boost immunization elicits robust neoantigen-specific CD8 + T cell responses and therapeutic protection in mouse tumor modelsOncoImmunologyDecember 31, 2026Pablo Morgado-Cáceres, Francisca Hofmann-Vega, Diego Figueroa, Juan Saavedra-Almarza, Felipe Gálvez-Cancino, Ximena Díaz, Evelyn Menares, Eduardo Roa, Sofia Hidalgo, Manuel Varas-Godoy, et al.Red blood cells from spleen and blood samples collected were lysed using RBC lysis buffer (Biolegend 420301). Skin was cut into small fragments and digested in 1 mL non-supplemented RPMI 1640 medium containing

(3) Surface-engineered milk-derived extracellular vesicles enable oral miRNA therapy against immunosuppressive pancreatic cancerMaterials Today BioOctober 1, 2026Youngri Ryu, Hochung Jang, Eun Hye Kim, Junha Lee, Byeongmin Park, Yewon Cho, Wonjun Kim, Hong-Yeol Yoon, Sun Hwa Kim, Dae-Hyuk Kweon, et al.#93040) in cold DPBS. Red blood cells were removed using red blood cell (RBC) lysis buffer (BioLegend, #420301). After cell counting, Fc Block (BD Biosciences, #553141) was added to prevent nonspecific antibody

(4) Bone targeted microenvironment actuated engineered exosomes for precision therapy of IBD associated bone lossBioactive MaterialsOctober 1, 2026Jiang Liu, Cong Wang, Taojin Feng, Siyu Chen, Siliang Ge, Yue Zhao, Ming Chen, Zhongqi Wang, Weier Bao, Pincong Fu, et al.out with sterile PBS, passed through a 70 μm cell strainer, and subjected to red blood cell lysis (420301, BioLegend). After washing, cells were resuspended in flow cytometry staining buffer. For Th17 analysis,

(5) Rituximab-Conjugated DART Nanoformulation for CD20-Targeted Drug Delivery in Non-Hodgkin LymphomaInt J NanomedicineSeptember 16, 2026Md Musavvir Mahmud, Byoungjae Kong, Daiheon Lee, Eduar Fernando Pinzon Burgos, Alonso Heredia, Jung Soo Suk, Anthony J Kim4°C. To remove erythrocytes, the resulting pellet was treated with 1× RBC lysis buffer (BioLegend, 420301) for 5 min on ice. The lysis reaction was neutralized by adding an excess volume of cold PBS. Following

(6) Myeloperoxidase promotes a tumorigenic microenvironment in non-small cell lung cancerRedox BiolSeptember 15, 2026Paulina Valadez-Cosmes, Kathrin Maitz, Anna Lagler, Oliver Kindler, Nejra Cosic Mujkanovic, Sofia Raftopoulou, Melanie Kienzl, Zala Nikita Juvan, Ana Santiso, Luka Brcic, et al.at 500 ×g (4 °C). The pellet was resuspended in red blood cell lysis buffer (BioLegend, catalog no. 420301, all human samples) and incubated for 3 min on ice with periodic shaking. The lysis process was neutralized

(7) A ROS-responsive platelet extracellular vesicle biomimetic nanosystem regulates CD36/Fyn-CXCL-NETosis pathway-mediated immune-inflammatory amplification in the treatment of transfusion-related acute lung injuryRedox BiolSeptember 6, 2026Jiani Gao, Ke Zhou, Yijiu Ren, Fengjing Yang, Minjue Shan, Yubing Fang, Changbo Sun, Weili Han, Chang Chen, Xuefei Hucell strainer to obtain single-cell suspensions. Red blood cells were lysed using RBC Lysis Buffer (#420301, BioLegend, USA), followed by two washes with PBS (300 ×g, 5 min each). The resulting cells were resuspended

(8) Nasal Nanoparticle Vaccine Induces a Cross-Strain T-Cell Immunity Against Toxoplasma gondiiPharmaceuticsSeptember 3, 2026Aurane Lecouffe, Thomas Bouillet, Bryan Thiroux, Amélie Degraeve, Anaïs-Camille Vreulx, Romain Magnez, Angelo Scuotto, Christophe Barnier-Quer, Didier Betbederrinsed with PBS, and erythrocytes were lysed using RBC Lysis Buffer (Biolegend, San Diego, CA, USA, cat. 420301) and resuspended in RPMI complete medium (RPMI 1640 supplemented with 10% SVF and 1% penicillin/streptomycin).

(9) Maternal obesity programs offspring metabolic dysfunction via small extracellular vesicle-mediated epigenetic remodelingNature CommunicationsAugust 25, 2026Huichen Song, Dameng Li, Lina Ma, Jin Wang, Nina Zhang, Chao Yan, Ke Zen, Xi Chen, Qipeng Zhang, Xiaohong Jiang, et al.suspension was filtered through a 70 μm cell strainer, treated with red blood cell lysis buffer (Cat#420301, BioLegend), centrifuged at 1200 rpm for 3 min, and resuspended in PBS for further use. Metabolic measurement The

(10) Impact of sialyltransferase deletion in mature T cells on control of subcutaneous and metastatic cancersGlycobiologyAugust 23, 2026Xiaoshuang Wang, James C Paulsonpreparation, the cell pellets were lysed using 1× red blood cell lysis buffer (diluted in ddH2O, Cat#420301, Biolegend) at room temperature for 2 min, then washed with PBS by centrifugation at 500 × g for 5 min

(11) MERTK inhibition cooperates with immunomodulatory cyclophosphamide to induce CXCL9+ monocyte-macrophage programming and durable antitumor immunity in triple negative breast cancerCancer Immunol ResAugust 17, 2026Alex J. Smith, Zachary Schrank, Nan Guan, Diego A. Pedroza, Sebastian J. Calderon, Xueying Yuan, Na Zhao, Zoe Gabriel, Yang Gao, Charlotte H. Rivas, et al.passed through a 40μm filter (Corning, 352340). This was followed by a brief RBC lysis (BioLegend, 420301). Both CD4+ and CD8+ T Cells were negatively selected using the EasySep Mouse T Cell Isolation Kit (STEMCELL Technologies

(12) Truncated Gpr114-based ultrasound-hypersensitive gene circuit for controlled expression of therapeuticsNature CommunicationsAugust 5, 2026Pengying Wu, Yubo Lai, Yang Liu, Zihang Li, Zhaoyou Liu, Lantian Wang, Jieyuan An, Yunnan Liu, Shuzhe Wei, Yue Sun, et al.(Absin, China). The collected cells were treated with red blood cell lysis buffer (BioLegend, Cat #420301) for 5 min. Subsequently, the samples were centrifuged at 400 × g for 10 min to remove the lysed red blood cells

(13) Vitamin B5 supports anti-PD1 response in HER2-positive gastric cancer and enhances interaction between naïve B cells and T cellsNature CommunicationsAugust 5, 2026Chaoye Wang, Jing Yang, Ming-Yu Lai, Jia-Chen Lu, Li-Qiong Yang, Zhi-Ying Wu, Hai-Yan Zhang, Fang Wang, Masami Yamamoto, Tetsuya Tsukamoto, et al.of the supernatant, the collected cells were resuspended with red blood cell lysis (Biolegend, Cat#420301) and incubated 4–5 min. After washing twice, the cell pellets were resuspended in MACS buffer (Miltenyi,

(14) Focused ultrasound thermal ablation and CD40 agonism reprograms breast tumor immunity to drive regression and memoryJ Immunother CancerAugust 4, 2026Zehra Ef Demir, Aeryon Kim, Beyzanur G Ak, Micaiah S Lee, Thomas Sherlock, Stefanyda O Maslova, Andrew T Thede, Matthew R DeWitt, Melanie Rutkowski, Natasha D Sheybanisamples were also subjected to hemolysis using BioLegend 1× red blood cell (RBC) lysis buffer (BioLegend #420301). Following RBC lysis, samples were quenched with complete media (RPMI or DMEM+10% FBS). Following,

(15) Pure Isolation, Culture, and Post-Injury Lineage Tracing of Mouse Visceral Pleural Mesothelial CellsAmerican Journal of Respiratory Cell and Molecular BiologyAugust 1, 2026Akikazu Endo, Yasunori Enomoto, Ryo Horiguchi, Shiori Meguro, Hideya Kawasaki, Isao Kosugi, Kenta Iijima, Shuji Takabayashi, Satoshi Baba, Toshihide IwashitaAfter centrifugation (400 g, 3 minutes), the cells were incubated with RBC lysis buffer (BioLegend, #420301) at room temperature for 3 minutes. Afterward, the cells were resuspended in DPBS with 3% FBS and centrifuged

(16) LRRK2 G2019S reprograms innate and adaptive immunity to drive context-dependent host defense outcomesFrontiers in Cellular NeuroscienceJuly 29, 2026Andrea R. Merchak, Mary K. Herrick, Madelyn C. Houser, Dina A. Moustafa, Joanna B. Goldberg, Jeremy M. Boss, Jacob Kohlmeier, Darcie M. Cook, Cody E. Keating, Jianjun Chang, et al.°C. Pellet was lysed for 5 min at room temperature using 1x red blood cell lysis buffer (BioLegend; 420301) and centrifuged for 10 min at 1670 g at 4 °C. The cells were washed and resuspended in PBS. Blood:

(17) Bacteria-mimicking cancer cells reprogram macrophages via multiple pattern recognition receptor pathways for cancer immunotherapySignal Transduct Target TherJuly 27, 2026Seoyoon Song, Dongjun Yu, Haneul Kang, Deborah Lee, Jiye Lee, Sungjun Kwak, Jinhee Park, Lifeng Kang, Stanislav Y. Emelianov, Jong-Ho Cha, et al.syringes and strained with a 70 μm cell strainer. Red blood cells were lysed with RBC lysis buffer (#420301, BioLegend). The resulting cells were handled as described in the “Cells and cell culture” section. Cells

(18) Dysregulated myelopoiesis and enhanced glutaminase-dependent neutrophil survival in preclinical obesityImmunohorizonsJuly 25, 2026Madison Babcock, Aric Lechner, Julia Drolet, Lynn Agostini, Ruth Weinstock, Norifumi Uraodifferential blood cell counts. Whole blood cells were treated with 1X RBC lysis buffer (Biolegend, cat. no. 420301). Following lysis and thorough washing, blood monocytes were stained with CD45 (2D1), LIN—CD3, CD19,

(19) Increased Expression of HLA-DR and CD69 on Peripheral CD4+ T Cells Predicts Better Clinical Outcomes in Cutaneous MelanomaImmunotargets TherJuly 22, 2026Ana Tomás, José Maximino, Hugo Nunes, Rute Salvador, Rafael Luís, Cheila Brito, Diana P Saraiva, Emanuel Gouveia, Carolina Pereira, Filipe Gonçalves, et al.without brake). PBMCs were washed and red blood cells (RBC) lysed with RBC lysis buffer 1× (BioLegend, 420301) for 15 min at room temperature. After a final wash step, PBMCs were cryopreserved at 2×106 cells/mL

(20) Proteolytic activation of executioner caspase-3 and -7 regulates different physiological processes in miceThe EMBO JournalJuly 21, 2026Noëlle Sieg, Johanna Stachelscheid, Besarta Thaqi, Tanja Schwab, Fabian Schorn, Daniela Hahn, Adrian Georg Simon, Yuri Tolkach, Nima Abedpour, Simon E Tröder, et al.,Thermo Fisher Scientific,EO0492 Cellpack,Sysmex,83400116 RPMI,Pan Biotech,P04-18500 DMEM,Bio&sell,BS.FG0435 RBC lysis buffer,BioLegend,420301 FBS,Biowest,S1810-500 ibidi µ-slide 8 Wellhigh Glass Bottom,ibidi,80807 L929,This study, Osmiumtetroxid,Science

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RBC Lysis Buffer (10X) from BioLegend

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RBC Lysis Buffer (10X) from BioLegend

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