Azure Cielo 3 Real Time qPCR System from Azure Biosystems, Inc

Product Specs
ItemAzure Cielo 3 Real Time qPCR System
CompanyAzure Biosystems, Inc
Price Get Pricing Get Quote for this Product
Catalog NumberAIQ1001
ApplicationsReal-Time PCR (qPCR), Multiplexing, Quantitative and qualitative gene expression analysis, miRNA analysis, Genetic mapping, Genetic fingerprinting, NGS library quantification, Up to 6 channel multiplex ability, Pathogen quantification
Wells96-wells
DetectionFiber optic scanning for 16 wells simultaneously for delivering speed and efficiency
Dimensions(WxDxH) 12 x 20 x 17 in. (30.5 x 50.8 x 43.2 cm)
Electrical Requirements100-240 VAC, 8A; Consumption: 0.8kW (800 Watts)
Volume10 µl to 150 µl
Temperature Range30 to 99.9 °C
Heating Rate6.0 °C/sec; 4°C/sec (Average)
Cooling Rate2°C/sec
Excitation Source3 standard optical modules for a wide variety of dyes
QuantityEA
Lid TypeHeated lid to 105 °C
Temperature Accuracy± 0.1 °C
Temperature Uniformity± 0.2 °C
SoftwareAzure Cielo Device Control software
Weight50 lbs (22.7 kg)
Regulatory StatusCE Certified, TUV Certified
Channels3
Time4.5 seconds to scan all 3 channels in all 96 wells
Validated ApplicationsTrue 3-channel multiplexing without need for any passive ROX reference Quantitative and qualitative gene expression analysis, miRNA analysis, NGS library quantification, Pathogen quantification
Interface10.3” touch screen
CapacityAIQ1001

Specifications/Features

12 gradient temperature zones
Heating and Cooling method : 6 Marlow Peltiers
Ability to auto-setup 12 gradient temperature zones with inter-temperature range of 0.1 °C
Connectivity: USB, WiFi, Ethernet (direct) Device can be remotely accessed via external laptop
Linux based operating system that allows smooth operations of qPCR experiments
Dye examples: SYBR Green/EvaGreen, FAM, VIC/JOE/HEX/CAL Fluor 540, CAL Fluor Orange 560, Cy5/LIZ/Mustang Purple

Features

Temperature gradient, Connectivity (USB, Wifi, Ethernet)

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Azure Biosystems, Inc

6747 Sierra Court

Suite A-B

Dublin, CA 94568

United States

Phone: (925) 307-7127

Company Profile

Website: www.azurebiosystems.com

(1) Unveiling Theranostics: Nanocomplex-Assisted Photodynamic Eradication of Aggressive Cancer Cells and Modulation of Tumor-Associated MacrophagesInt J NanomedicineAugust 8, 2025Austeja Butkute, Evelina Kazlauske, Agata Mlynska, Emile Peciukaityte, Vitalijus Karabanovas, Ricardas Rotomskis, Simona Steponkienein accompanying instructions. Quantitative PCR reactions were performed in 96-well plates using the Azure Cielo 3 Real Time qPCR System thermocycler (Azure Biosystems, Dublin, CA, USA). The reaction volume of 10 μL contained 2.5 µL of the

(2) Reference genes validation in tear fluid for RNA analysis in ocular surface diseaseSci RepJuly 9, 2025Diego A. Ojeda, Hooman Ahmadzadeh, Sarp Orgul, Angela Gomez Bedoya, Swati Gupta, Katherine Held, Swarup S. Swaminathan, Alfonso L. Sabatergene-specific Taqman assays. Each sample was conducted in duplicates in a 20 µL reaction volume on the Azure Cielo 3 Real-Time PCR System (Azure Biosystems). The PCR protocol commenced with an initial step of 2 min at

(3) Coordinated neuron-specific splicing events restrict nucleosome engagement of the LSD1 histone demethylase complexCell ReportsJanuary 28, 2025Robert S. Porter, Sojin An, Maria C. Gavilan, Masayoshi Nagai, Yumie Murata-Nakamura, Bo Zhou, Katherine M. Bonefas, Olivier Dionne, Jeru Manoj Manuel, Joannie St-Germain, et al.instructions. The expression of different target genes was validated by quantitative PCR (qPCR) using the Azure Cielo 3 system (Azure Biosystems, Dublin, CA, USA). The reactions were performed with the PerfeCta SYBR Green

(4) A novel isoform of Tensin1 promotes actin filament assembly for efficient erythroblast enucleationbioRxivDecember 21, 2024Arit Ghosh, Megan Coffin, Dimitri M. Diaz, Sarah Barndt, Vincent Schulz, Patrick Gallagher, Su Hao Lo, Velia M. Fowlerthe manufacturer’s instructions in a 96-well fast read plate (Genesee Scientific, 24-310) using the Azure Cielo 3 Real Time PCR System (Azure Biosystems, AIQ030). Transient transfection of HEK 293T cells with HA-eTNS1

(5) Nontypeable Haemophilus influenzae challenge during gammaherpesvirus infection enhances viral reactivation and latencyVirologySeptember 1, 2024Nicholas P. Huss, Sheikh Tahir Majeed, Brandon M. Wills, Vera L. Tarakanova, Kenneth L. Brockman, Christopher N. Jondlewas assessed in triplicate, along with corresponding negative RT reactions by real time PCR using the Azure Cielo 3 Real Time PCR System (Azure Biosystems, Dublin, CA). The relative abundance of each cDNA in the sample

(6) Defining Melanoma Immune Biomarkers—Desert, Excluded, and Inflamed Subtypes—Using a Gene Expression Classifier Reflecting Intratumoral Immune Response and Stromal PatternsBiomoleculesJanuary 31, 2024Agata Mlynska, Jolita Gibavičienė, Otilija Kutanovaitė, Linas Senkus, Julija Mažeikaitė, Ieva Kerševičiūtė, Vygantė Maskoliūnaitė, Neda Rupeikaitė, Rasa Sabaliauskaitė, Justina Gaiževska, et al.Scientific, Waltham, MA, USA) according to manufacturer’s guidelines. qPCR was performed in triplicate in Azure Cielo 3 Real-Time PCR System (Azure Biosystems, Dublin, CA, USA). In one reaction of ten microliters volume,

(7) The reduction of environmentally abundant iron oxides by the methanogen Methanosarcina barkeriFrontiers in MicrobiologyJuly 20, 2023Efrat Eliani-Russak, Zohar Tik, Shaked Uzi-Gavrilov, Michael M. Meijler, Orit Sivanevaluated using a nanodrop spectrophotometer (ND1000). Real-time PCR amplifications were conducted using an Azure Cielo 3 system (Azure biosystems, United States) in 20 μL volumes, with each standard reaction mix containing

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Azure Cielo™ Real-Time PCR System
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