Propidium Iodide Solution from BioLegend

Product Specs
ItemPropidium Iodide Solution
CompanyBioLegend
Price $54.00Supplier Page View Company Product Page
Catalog Number421301
ApplicationsFC - Quality tested
Quantity2 mL
FormatPhosphate-buffered saline, pH 7.2, containing 0.09% sodium azide
Concentration0.5mg/ml

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) The transcriptional cortical response to daily torpor in Phodopus sungorusLife Sci AllianceJuly 29, 2026Lukas C Bal, Marcel S Woo, Nicola Rothammer, Elena Haugg, Christina Mayer, Annika Herwig, Manuel A Friesestaining with AF647-labelled NeuN antibody (1:500, Ab190565; Abcam) and 0.25 μg ml−1 propidium iodide (421301; BioLegend) as a DNA counterstain. Staining was performed on ice for approximately 10–20 min. NeuN+

(2) Simultaneous measurement of target and effector cell death, apoptosis, and proliferation in cell therapy development using flow cytometrySTAR ProtocJuly 23, 2026Kristian Thomson, Kendra Klatt, Niklas Engels, Camille Dourlens, Daniel Schäfer, Melissa Quadflieg, Hannah Johanssen, Raphael Koch, Jürgen Wienands, Gerald Wulfstreptomycin)",PAN Biotech,P06-07100 Precision Count Beads,BioLegend,424902 Propidium iodide (500 μg/mL),BioLegend,421301 RPMI 1640 with L-Glutamine,Anprotec,AC-LM-0058 Sodium pyruvate 100 mM (100 ×),PAN Biotech,P04-43100 Staurosporine

(3) CD157+ vascular endothelial stem cells represent a conserved subpopulation with an angiogenic gene expression profileStem Cell ReportsJuly 14, 2026Tomohiro Iba, Taku Wakabayashi, Rie Ito, Ai Sugawara, Satoshi Fujimura, Mika Sawane, Kazuaki Yoshioka, Aya Matsui, Jun-ichi Morishige, Naoto Nagata, et al.Pharma,NDC 00078-0109 DAPI,Thermo Fisher Scientific,Cat# D1306 Propidium Iodide Solution,BioLegend,Cat# 421301 Dispase II,Thermo Fisher Scientific,Cat# 17105041 Collagenase,Wako,Cat# 034-22363 Collagenase Type

(4) Ocular IL-1α/IFN-γ Delay the Corneal Wound Healing in Rheumatoid Arthritis Through Mitochondrial Dysfunction and NLRP3 Inflammasome ActivationInvest Ophthalmol Vis SciJuly 8, 2026Zhiwei Ren, Dingwen Cao, Jiayu Meng, Yanling Dong, Lei Xu, Liuli Wang, Chao Wei, Jiang Biancorresponding isotype control IgGs for 30 minutes at 4°C in the dark. Propidium iodide solution (Cat. No. 421301; BioLegend) was used to exclude dead cells. Samples were acquired on a CytoFLEX flow cytometer (CytoFLEX;

(5) Early glymphatic failure in AppNL-F knock-in mice is linked to parenchymal border macrophages lossBrainJuly 7, 2026Na Liu, Yiyi Yang, Marios Kritsilis, Kelley M Swanberg, Chenchen Liu, Xuanhui Liu, Brecht Moonen, Tomas Deierborg, Per Nilsson, Stina Syvänen, et al.software (BD Biosciences). Viable cells were identified using propidium iodide (PI) staining (1:1000, 421301, BioLegend), and PI-negative cells were gated for further analysis. Antibodies used included: anti-mouse

(6) Adipocyte OX40L promotes adipose T cell activation and insulin resistance in obesityExp Mol MedJuly 6, 2026Jianfeng Song, Qin Zeng, Yayi Jiao, Xiaoxiao Sun, Limin Xie, Jingpei Qiu, Yujin Ding, Wanyu Hu, Fanqi Wang, Biling Huang, et al.PBS, transferred to flow cytometry tubes, and stained with 0.6 μL propidium iodide (PI, Biolegend, Cat#421301) for viability assessment prior to flow cytometric analysis. The instrument used for flow cytometry

(7) Cell-autonomous control of CAR signaling and receptor shedding via ADAM17-mediated proteolysisCellJune 25, 2026Jeremy R. Bjelajac, Adrià Cañellas-Socias, Preeti Nehra, Kevin Reynolds, Meena Malipatlolla, Naiara Martinez Martinez Velez, Diane C. Manjarrez, Katie Ho, Peng Xu, Jennifer L. Hamad, et al.1mg,MedChemExpress,HY-16657 Dasatinib (25mg),MilliporeSigma,CDS023389 Propidium Iodide,Biolegend,421301 PerCP/Cyanine5.5 Annexin V,Biolegend,640936 "Recombinant Human B7-H3 Fc Chimera Protein, CF",R&D Systems,1027-B3-100 "Human

(8) Neuroglial P2Y1 receptor signalling differentially contributes to inflammatory neurodegenerationJ NeuroinflammationJune 13, 2026Charlotte Schubert, Ricardo Lopes Fonseca, Alexandros Hadjilaou, Vanessa Vieira, Karoline Degenhardt, Anna Lena Seemann, Alice Hakimy, Jana K. Sonner, Peter Ludewig, Tim Magnus, et al.the neuronal marker MAP2 (ABCAM, Cat. #AB5392) and propidium iodide (PI; 1:1,000; BioLegend, Cat. #421301). Neuronal nuclei served as region of interest (ROI) for quantifying DAPI uptake by mean fluorescence

(9) Metabolic and cellular physiological differences between embryonic breast and leg muscle satellite cells in chickensPoultry ScienceJune 1, 2026Jongryun Kim, Jeongeun Lee, Dongjin Yu, Leecheon Kim, Darae Kang, Jinryong Park, Guybong Song, Hakkyo Lee, Kwanseob Shimμg/mL RNase A (70856, Sigma-Aldrich, St. Louis, MO, USA) in PBS and 20 μg/mL propidium iodide solution (421301, BioLegend, San Diego, CA, USA). The prepared samples were transferred to FACS tubes and analyzed by

(10) DKC1 promotes colorectal cancer progression and therapy resistance by dysregulating sphingolipid biosynthesisNat CommunMay 18, 2026Umar K. Khan, Ayush Goel, Shivansh Nigam, Nazia Chaudhary, Ayush Praveen, Anjan Roy, Fabeha Fatima, Dilpreet K. Sawhney, Afiya Dalwai, Akshay Shendre, et al.and fixed with 70% ethanol. Subsequently, they were stained with propidium iodide (BioLegend, Cat # 421301) as per the manufacturer’s protocol before acquisition. Cell cycle distribution was assessed using an

(11) miR-4772-3p serves as a potential diagnostic and prognostic biomarker in septic acute kidney injury by targeting CISD2Ren FailMay 17, 2026Qiang Chen, Gaoshang Zhuflow cytometry after double staining with Annexin V-FITC (640906, BioLegend, USA) and propidium iodide (PI) (421301, BioLegend, USA). HK-2 cells were harvested, rinsed with ice-cold PBS, and resuspended in binding buffer.

(12) Enhancing the Therapeutic Profile of Dapagliflozin: Chitosan Nanoparticle Encapsulation for Intestinal ApplicationsFASEB BioadvMay 14, 2026Agni Klonari, Antrea-Maria Athinodorou, Eirini Papanikolaou, Anastasia Skonta, Myrto G. Bellou, Lampros Lakkas, Konstantinos I. Tsamis, Georgios S. Markopoulos, Haralambos Stamatis, Petros Bozidis, et al.100 μL Annexin V Binding Buffer (Biolegend 422201). Annexin V (Biolegend 640906) and PI (Biolegend 421301) (5 μg/mL each) were added under dark conditions, and samples were gently vortexed and incubated at

(13) Targeting WNK1 Releases Differentiation Block in Acute Myeloid LeukemiabioRxivApril 30, 2026Jordan D. Cress, Emily M. Katoni, Parameswaran Ramakrishnanethanol for 30 minutes at 4°C then washed twice with PBS. 20 μg/mL Propidium iodide (PI) (BioLegend, 421301) and 100 μg/mL RNase (Sigma, R6148) were added to the cell suspension. Samples were incubated at room

(14) miR-99b-5p inhibition drives apoptosis and tumor shrinkage in triple-negative breast cancer: functional characterization through AGO2-RIP-seq and mechanistic insightsFrontiers in OncologyApril 22, 2026Senem Noyan, Kubra Nur Kaplan Ilhan, Muge Ocal Demirtas, Begum Karaoglu Dallı, Bora Ergin, Rabia Sen, Hakan Gurdal, Bala Gur Dedeoglu106 cells/ml. Annexin V-FITC (5 µl, 640906, BioLegend) and propidium iodide (PI) (5 µl, 0.5 µg/µl, 421301, BioLegend) were then added to the cell suspension and incubated for 15 minutes at room temperature

(15) The PPTC7/BNIP3/NIX axis induces cGAS/STING-mediated senescence and augments CAR-T efficacy by repressing tumor-intrinsic mitophagyJournal of Experimental & Clinical Cancer ResearchApril 18, 2026Kuai Yu, Xinxin Xiong, Bo Lu, Yaqun Wang, Tiantian Sun, Mei Xie, Xiaojun Xu, Yuanbin Song, Wei Xiaotreatment and co-culture with CAR-T, AMO1 and RPMI8226 cells were stained with propidium iodide (PI; 421301, BioLegend, USA) at a final concentration of 0.2 µg/mL. Cells were collected, washed once with phosphate-buffered

(16) Excessive pyroptosis mediates the exacerbation of pneumonia caused by low-lethality influenza virus and secondary MRSA co-infectionCell Death DiscoveryApril 2, 2026Zi-Chen Tian, Yang Liu, Yi-Jun Niu, Xin Ai, Su-Ya Lao, Wei-Ming Xu, Xiao-Tong Lin, Cheng-Jie Xia, Zhi-Xuan Cai, Hai-Yan Zhu, et al.Antibody (ABclonal, A27478); anti-mouse CD11b Antibody (BioLegend, Cat#101205); PI (BioLegend, Cat#421301); Annexin V-FITC (Beyotime, C1062M)] and fixed with 4% paraformaldehyde. The suspensions were then washed

(17) Immune Modulatory Oxysterols Produced from Cholesterol-Containing Lipid Nanoparticles Regulate Tumor GrowthACS NanoMarch 31, 2026Patricia Ines Back, Shadan Modaresahmadi, Jalpa Patel, Indhumathy Subramaniyan, Vindhya Edpuganti, Md. Rakibul Islam, Seonjuti Chowdhury, Adam J. Carr, Qisheng Zhang, William C. Putnam, et al.1 × 106 cells were stained with APC Annexin V (Cat. #640920, BioLegend) and propidium iodide (Cat. #421301, BioLegend). Apoptosis and necrosis controls were used to set up the gating strategies. LSR Fortessa

(18) Dose-dependent effects of mitoquinone on mitochondrial function and post-thaw quality of cryopreserved canine spermVeterinary WorldMarch 31, 2026Abbas Farshad, Emilia Diel, Axel Wehrend20°C–22°C in the dark, followed by an additional incubation with 5 μL propidium iodide (PI, Cat. No. 421301, BioLegend Inc.) for 15 min. Before analysis, samples were diluted with 400 μL binding buffer, and at

(19) Spatial multi-omics characterization of neuroblastoma reveals ferroptosis-associated metabolic features in high-risk tumorsGenome MedicineMarch 31, 2026Cui Tu, Chin Wee Tan, James Monkman, Ana Clara Almeida, Gabrielle Antonio-Carreon, Natacha Omer, Giaan Hull, Kimberly Chung, Ahmed M. Mehdi, Antonietta Salerno, et al.Cells were then treated with 10 µM of ML210, FIN56 or FINO2 and propidium Iodide (PI) (BioLegend, #421301) was added to assess cell viability. The plate was placed in the Sartorius Incucyte S3 live cell analysis

(20) Symbiotic exclusivity between CLOCK and TFPI2 drives stemness and immunosuppression in glioblastoma modelsJ Clin InvestMarch 17, 2026Fei Zhou, Lizhi Pang, Yang Liu, Fatima Khan, Peiwen Chenand stained with Apotracker (1:10 dilution). Then 5 μL propidium iodide (PI) solution (BioLegend, # 421301) was added to label the necrotic and late apoptotic cells. Cells were washed with PBS three times. Fluorescein

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1/20/2021PI is a Simple but Effective live/dead Stain Anonymous ReviewTo further characterize cell populations it is best to initially gate on live cells. in this experiment I used PI as my live/dead stain.

Propidium Iodide Solution from BioLegend

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Propidium Iodide Solution from BioLegend

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