Propidium Iodide Solution from BioLegend

Product Specs
ItemPropidium Iodide Solution
CompanyBioLegend
Price $54.00Supplier Page View Company Product Page
Catalog Number421301
ApplicationsFC
Quantity2ML
FormatPhosphate-buffered saline, pH 7.2, containing 0.09% sodium azide
Concentration0.5000mg/mL

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Morphological and Cytotoxic Effects of Metal Salts on Osteoblast Cells in Three-Dimensional Spheroidal CulturesJ ToxicolSeptember 26, 2026Misael Vargas-López, José Luis Castrejón-Flores, Ángel Ernesto Bañuelos-Hernández, Fernando Gómez-Chávez, Ricardo García-Ruiz, Ma. Lourdes Rojas-Morales, Elizabeth Pérez-Hernández, Nury Pérez-Hernándezcontaining 80‐μg/mL fluorescein diacetate (FDA) (D6883, Sigma‐Aldrich) and 200‐μg/mL propidium iodide (PI) (421301, BioLegend). Samples were incubated in the dark at 37°C with 5% CO2 for 30 min. Following incubation,

(2) PBMC-derive eNK cells was armed and promoted CAR-like response against tumors through armed with 16Fc21, a novel bispecific fusion protein for NK cellsFront ImmunolSeptember 3, 2026Yangyang Li, Yehai Wang, Ximeng Zhang, Yan Fan, Lina Pan, Jing Wu, Weihua Xiaoco-incubated with K562 cells at E:T=1:1 for 24 hours at 37 °C. Propidium iodide (BioLegend, Cat. No. 421301) was added for the last 5 minutes at room temperature to label dead cells. Specific killing of K562 cells

(3) Targeting 3-mercaptopyruvate sulfurtransferase selectively eliminates colorectal cancer stem cellsRedox BiolSeptember 2, 2026Kelly Ascenção, Olivia Oravecz, Csaba SzaboAPC-conjugated Annexin V (BioLegend, cat. no. 640920) and propidium iodide (PI; BioLegend, cat. no. 421301) in Annexin V binding buffer (BioLegend, cat. no. 422201) according to the manufacturer's instructions.

(4) P-selectin delineates conserved functional heterogeneity in early hematopoietic stem cell aging in humans and miceThe EMBO JournalAugust 5, 2026Chong Yang, Tong Wang, Yue Chai, Lisha Wang, Yixuan Wang, Weili Wang, Fang Dong, Toshio SudaMitoTracker™ Green FM,MedChemExpress,HY-135056 TMRE,Invitrogen,T669 CellROX,Invitrogen,C10443 MitoSOX,Invitrogen,M36005 Hoechst,Miltenyi Biotec,130-111-569 Propidium Iodide,Biolegend,421301 Software,Software,Software Prism 10,Graphpad Software,https://www.graphpad.com Endnote 8.2,Clarivate,https://endnote.com/ FlowJo

(5) The transcriptional cortical response to daily torpor in Phodopus sungorusLife Sci AllianceJuly 29, 2026Lukas C Bal, Marcel S Woo, Nicola Rothammer, Elena Haugg, Christina Mayer, Annika Herwig, Manuel A Friesestaining with AF647-labelled NeuN antibody (1:500, Ab190565; Abcam) and 0.25 μg ml−1 propidium iodide (421301; BioLegend) as a DNA counterstain. Staining was performed on ice for approximately 10–20 min. NeuN+

(6) Simultaneous measurement of target and effector cell death, apoptosis, and proliferation in cell therapy development using flow cytometrySTAR ProtocJuly 23, 2026Kristian Thomson, Kendra Klatt, Niklas Engels, Camille Dourlens, Daniel Schäfer, Melissa Quadflieg, Hannah Johanssen, Raphael Koch, Jürgen Wienands, Gerald Wulfstreptomycin)",PAN Biotech,P06-07100 Precision Count Beads,BioLegend,424902 Propidium iodide (500 μg/mL),BioLegend,421301 RPMI 1640 with L-Glutamine,Anprotec,AC-LM-0058 Sodium pyruvate 100 mM (100 ×),PAN Biotech,P04-43100 Staurosporine

(7) Trained immunity links hematopoietic stem cell aging to aging-associated inflammationNature AgingJuly 16, 2026Wei-Chieh Mu, Marine Barthez, Yufan Feng, Yibing Chen, Chih Ling Wang, Jing-Yi Huang, Jie Li, Paul Lanhart, K. Claire Tsuei, Yang Joon Kim, et al.mixture for HSCs for 20 min at 4 °C, washed and stained with 0.5 µg ml−1 propidium iodide (BioLegend, 421301). Live HSCs were sorted using FACSAria (BD). Complete blood count Peripheral blood was collected via

(8) CD157+ vascular endothelial stem cells represent a conserved subpopulation with an angiogenic gene expression profileStem Cell ReportsJuly 14, 2026Tomohiro Iba, Taku Wakabayashi, Rie Ito, Ai Sugawara, Satoshi Fujimura, Mika Sawane, Kazuaki Yoshioka, Aya Matsui, Jun-ichi Morishige, Naoto Nagata, et al.Pharma,NDC 00078-0109 DAPI,Thermo Fisher Scientific,Cat# D1306 Propidium Iodide Solution,BioLegend,Cat# 421301 Dispase II,Thermo Fisher Scientific,Cat# 17105041 Collagenase,Wako,Cat# 034-22363 Collagenase Type

(9) Ocular IL-1α/IFN-γ Delay the Corneal Wound Healing in Rheumatoid Arthritis Through Mitochondrial Dysfunction and NLRP3 Inflammasome ActivationInvest Ophthalmol Vis SciJuly 8, 2026Zhiwei Ren, Dingwen Cao, Jiayu Meng, Yanling Dong, Lei Xu, Liuli Wang, Chao Wei, Jiang Biancorresponding isotype control IgGs for 30 minutes at 4°C in the dark. Propidium iodide solution (Cat. No. 421301; BioLegend) was used to exclude dead cells. Samples were acquired on a CytoFLEX flow cytometer (CytoFLEX;

(10) Non-genotoxic transplantation and in vivo selection through epitope editingNatureJuly 8, 2026Gabriele Casirati, Andrea Cosentino, Marta Freschi, Jing Zeng, Adele Mucci, Sébastien Levesque, Nola Neri, Enrico Drago, Viola Carzaniga, Francesco Romano, et al.LiveDead yellow (Invitrogen L34959), 7-AAD (Biolegend 420404), or propidium iodide (PI, Biolegend, 421301) staining. Immunophenotyping of in vitro base-edited CD34+ HSPCs was evaluated by staining for 30 min

(11) Early glymphatic failure in AppNL-F knock-in mice is linked to parenchymal border macrophages lossBrainJuly 7, 2026Na Liu, Yiyi Yang, Marios Kritsilis, Kelley M Swanberg, Chenchen Liu, Xuanhui Liu, Brecht Moonen, Tomas Deierborg, Per Nilsson, Stina Syvänen, et al.software (BD Biosciences). Viable cells were identified using propidium iodide (PI) staining (1:1000, 421301, BioLegend), and PI-negative cells were gated for further analysis. Antibodies used included: anti-mouse

(12) Adipocyte OX40L promotes adipose T cell activation and insulin resistance in obesityExp Mol MedJuly 6, 2026Jianfeng Song, Qin Zeng, Yayi Jiao, Xiaoxiao Sun, Limin Xie, Jingpei Qiu, Yujin Ding, Wanyu Hu, Fanqi Wang, Biling Huang, et al.PBS, transferred to flow cytometry tubes, and stained with 0.6 μL propidium iodide (PI, Biolegend, Cat#421301) for viability assessment prior to flow cytometric analysis. The instrument used for flow cytometry

(13) Cell-autonomous control of CAR signaling and receptor shedding via ADAM17-mediated proteolysisCellJune 25, 2026Jeremy R. Bjelajac, Adrià Cañellas-Socias, Preeti Nehra, Kevin Reynolds, Meena Malipatlolla, Naiara Martinez Martinez Velez, Diane C. Manjarrez, Katie Ho, Peng Xu, Jennifer L. Hamad, et al.1mg,MedChemExpress,HY-16657 Dasatinib (25mg),MilliporeSigma,CDS023389 Propidium Iodide,Biolegend,421301 PerCP/Cyanine5.5 Annexin V,Biolegend,640936 "Recombinant Human B7-H3 Fc Chimera Protein, CF",R&D Systems,1027-B3-100 "Human

(14) Neuroglial P2Y1 receptor signalling differentially contributes to inflammatory neurodegenerationJ NeuroinflammationJune 13, 2026Charlotte Schubert, Ricardo Lopes Fonseca, Alexandros Hadjilaou, Vanessa Vieira, Karoline Degenhardt, Anna Lena Seemann, Alice Hakimy, Jana K. Sonner, Peter Ludewig, Tim Magnus, et al.the neuronal marker MAP2 (ABCAM, Cat. #AB5392) and propidium iodide (PI; 1:1,000; BioLegend, Cat. #421301). Neuronal nuclei served as region of interest (ROI) for quantifying DAPI uptake by mean fluorescence

(15) GPR15-guided CD8+ T regulatory cells control intestinal inflammationNatureJune 8, 2026Jing Cui, Zuojia Chen, Yan H. Cheng, Jia Nie, Maria H. Zhu, Chen Xiang, Samuel Chauvin, Wiem Lassoued, Can Liu, Kartika Padhan, et al.CD8α+ T cells were removed and macrophages were stained with propidium iodide (BioLegend, catalogue no. 421301) for 5 min before imaging on a ZEISS Axio Vert.A1 TL/FL-LED inverted microscope using ZEN software (v.3.10,

(16) Organelle contact reorganization drives calcium-dependent autophagy under proteostatic stressAutophagyJune 6, 2026Yubin Ko, Minjeong Ko, Marius Ueffing, Hye Jin Kang, Ho Jeong Kwon(Sigma-Aldrich, T8154); ANXA5/annexin V-FITC (BioLegend, 640906); propidium iodide (PI) (BioLegend, 421301); Matrigel (Corning, 356234); Matrigel (Corning, 356231); VEGF/vascular endothelial growth factor (R&D Systems

(17) Metabolic and cellular physiological differences between embryonic breast and leg muscle satellite cells in chickensPoultry ScienceJune 1, 2026Jongryun Kim, Jeongeun Lee, Dongjin Yu, Leecheon Kim, Darae Kang, Jinryong Park, Guybong Song, Hakkyo Lee, Kwanseob Shimμg/mL RNase A (70856, Sigma-Aldrich, St. Louis, MO, USA) in PBS and 20 μg/mL propidium iodide solution (421301, BioLegend, San Diego, CA, USA). The prepared samples were transferred to FACS tubes and analyzed by

(18) DKC1 promotes colorectal cancer progression and therapy resistance by dysregulating sphingolipid biosynthesisNat CommunMay 18, 2026Umar K. Khan, Ayush Goel, Shivansh Nigam, Nazia Chaudhary, Ayush Praveen, Anjan Roy, Fabeha Fatima, Dilpreet K. Sawhney, Afiya Dalwai, Akshay Shendre, et al.and fixed with 70% ethanol. Subsequently, they were stained with propidium iodide (BioLegend, Cat # 421301) as per the manufacturer’s protocol before acquisition. Cell cycle distribution was assessed using an

(19) miR-4772-3p serves as a potential diagnostic and prognostic biomarker in septic acute kidney injury by targeting CISD2Ren FailMay 17, 2026Qiang Chen, Gaoshang Zhuflow cytometry after double staining with Annexin V-FITC (640906, BioLegend, USA) and propidium iodide (PI) (421301, BioLegend, USA). HK-2 cells were harvested, rinsed with ice-cold PBS, and resuspended in binding buffer.

(20) Enhancing the Therapeutic Profile of Dapagliflozin: Chitosan Nanoparticle Encapsulation for Intestinal ApplicationsFASEB BioadvMay 14, 2026Agni Klonari, Antrea-Maria Athinodorou, Eirini Papanikolaou, Anastasia Skonta, Myrto G. Bellou, Lampros Lakkas, Konstantinos I. Tsamis, Georgios S. Markopoulos, Haralambos Stamatis, Petros Bozidis, et al.100 μL Annexin V Binding Buffer (Biolegend 422201). Annexin V (Biolegend 640906) and PI (Biolegend 421301) (5 μg/mL each) were added under dark conditions, and samples were gently vortexed and incubated at

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1/20/2021PI is a Simple but Effective live/dead Stain Anonymous ReviewTo further characterize cell populations it is best to initially gate on live cells. in this experiment I used PI as my live/dead stain.

Propidium Iodide Solution from BioLegend

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Propidium Iodide Solution from BioLegend

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