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Biocompare
Products
Plasmid Purification Kits (Mini)
PureYield Plasmid Miniprep System
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PureYield Plasmid Miniprep System
PureYield Plasmid Miniprep System
PureYield Plasmid Miniprep System from Promega
(2)
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Citations:
(65)
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PureYield Plasmid Miniprep System
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Description
The PureYield Plasmid Miniprep System is designed to rapidly isolate highly pure plasmid DNA. Yields up to 15ug of plasmid DNA from 600ul to 3ml of bacterial culture
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
PureYield Plasmid Miniprep System
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
A1223
Time
> 10 minutes
Yield
purify 1.5–7.5 µg of plasmid DNA
Quantity
100 preps
Method
silica membrane column
Source
Bacterial
Nucleic Acid Type
Plasmid DNA
Applications
purification
Volume
If higher yields are desired-1.5–3.0ml of culture
low-copynumber plasmids- 3ml culture volumes are recommended
Add to Compare List
Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (65)
(1)
Activation of GPR44 decreases severity of myeloid leukemia via specific targeting of leukemia initiating stem cells
Cell reports
July 25, 2023
Qian F, Nettleford SK, Zhou J, Arner BE, Hall MA, Sharma A, Annageldiyev C, Rossi RM, Tukaramrao DB, Sarkar D,
et al
.
8802–6848-74
Plasmid Maxi Kit (25),
Qiagen
,Cat#:
12163
PureYield™ Plasmid Miniprep System
,
Promega
,Cat#
A1223
Wizard® SV Gel and PCR Clean-Up System
(50 preps),
Promega
,Cat#
A9281
RNA 6000 Nano Kit,
Agilent
,Cat#
(2)
Accurate profiling of full-length Fv in highly homologous antibody libraries using UMI tagged short reads
Nucleic Acids Research
June 23, 2023
Itay Levin, Marek Štrajbl, Yair Fastman, Dror Baran, Shir Twito, Jessica Mioduser, Adi Keren, Sharon Fischman, Michael Zhenin, Guy Nimrod,
et al.
collected, and the plasmid DNA was isolated using
PureYield™ Plasmid Miniprep System
(
Promega
, Cat. No.
A1222
). The library DNA amount was quantified and then diluted in a series of dilution rounds in order to
(3)
Localized modulation of DNA supercoiling, triggered by the Shigella anti-silencer VirB, is sufficient to relieve H-NS-mediated silencing
Nucleic Acids Research
May 8, 2023
Michael A Picker, Monika M A Karney, Taylor M Gerson, Alexander D Karabachev, Juan C Duhart, Joy A McKenna, Helen J Wing
the
PureYield Plasmid Miniprep System
according to the manufacturer's directions (
Promega
, Cat. No.
A1222
). For in vivo supercoiling
assays
in
Shigella
, the PicsP-lacZ reporter was introduced into the wild-type
(4)
Prevalence of Extended-Spectrum β-Lactamases in Multidrug-Resistant Klebsiella pneumoniae Isolates in Jordanian Hospitals
Journal of Epidemiology and Global Health
April 24, 2023
Suhaila A. Al-Sheboul, Ghina S. Al-Madi, Brent Brown, Wail A. Hayajneh
sample.
Plasmid extraction
was performed using
Promega
PureYieldTM
Plasmid Miniprep
System (Catalogue #
A1223
, USA) and according to the manufacturer’s instructions. After
plasmid extraction
, the concentration
(5)
Preparation of chaperone-loaded neural stem cell-derived extracellular vesicles to reduce protein aggregation in Huntington’s disease cellular models
STAR Protocols
March 1, 2023
Bhagyashree S. Joshi, Inge S. Zuhorn
,
GE Healthcare
,
RPN2232
QIAquick PCR & Gel Cleanup Kit (100
),
Qiagen
,
28506
PureYield™ Plasmid Miniprep System
,
Promega
,
A1222
,,
Experimental models
: Cell lines,
Experimental models
: Cell lines,
Experimental models
: Cell lines ,,
Human
(6)
Role of human HSPE1 for OPA1 processing independent of HSPD1
iScience
February 17, 2023
Yeung N, Murata D, Iijima M, Sesaki H.
commercial
assays
,, RNAeasy Mini Kit,
Qiagen
,Cat#
74106
Pure Yield Plasmid Mini Prep System,
Promega
,Cat#
A1222
PowerUp SYBR Green Master Mix
,
Applied Biosystems
,Cat#
A25742
ReadyScript
cDNA Synthesis
Mix,Sigma-Aldrich,Cat#
(7)
Do Amino Acid Antiporters Have Asymmetric Substrate Specificity?
Biomolecules
February 6, 2023
Gregory Gauthier-Coles, Stephen J. Fairweather, Angelika Bröer, Stefan Bröer
propagated before isolating the plasmids using the Wizard Plus SV Miniprep
DNA purification
system (
Promega
;
A1223
). Plasmids were then sent to the Biomolecular Resource Facility (ANU) for
Sanger sequencing
.
In vitro transcription
(8)
A noncanonical RNA-binding domain of the fragile X protein, FMRP, elicits translational repression independent of mRNA G-quadruplexes
Journal of Biological Chemistry
December 1, 2022
MaKenzie R. Scarpitti, Julia E. Warrick, Evelyn L. Yoder, Michael G. Kearse
(
Thermo Fisher
#
C404006
), purified using the PureYield
Plasmid Miniprep
or
Midiprep
Systems (
Promega
#
A1222
and
A2495
), and validated by
Sanger sequencing
at The Ohio State University Comprehensive
Cancer
Center
(9)
Membrane phosphoinositides regulate GPCR-β-arrestin complex assembly and dynamics
Cell
November 23, 2022
John Janetzko, Ryoji Kise, Benjamin Barsi-Rhyne, Dirk H. Siepe, Franziska M. Heydenreich, Kouki Kawakami, Matthieu Masureel, Shoji Maeda, K. Christopher Garcia, Mark von Zastrow,
et al.
E2621X
NucleoBond Xtra Midi Plus
,
Macherey-Nagel
,Cat# 740412.50
PureYield Plasmid Miniprep System
,
Promega
,Cat#
A1222
FastGene Plasmid Mini Kit,Nippon Genetics,Cat# FG-90502
MycoAlert Mycoplasma Detection Kit
,
Lonza
,Cat#
(10)
Genetic Modulation of the GLUT1 Transporter Expression—Potential Relevance in Complex Diseases
Biology (Basel)
November 16, 2022
Anna Kulin, Nóra Kucsma, Balázs Bohár, Botond Literáti-Nagy, László Korányi, Judit Cserepes, Anikó Somogyi, Balázs Sarkadi, Edit Szabó, György Várady
ultra-competent cells and purified using the
PureYield Plasmid Miniprep System
(
Promega
, Madison, WI, USA,
A1223
). To ensure the
ligation
was correct, we sent the constructs for
Sanger sequencing
. 2.5.
Cell Culture
,
(11)
Relating individual cell division events to single-cell ERK and Akt activity time courses
Scientific Reports
October 27, 2022
Alan D. Stern, Gregory R. Smith, Luis C. Santos, Deepraj Sarmah, Xiang Zhang, Xiaoming Lu, Federico Iuricich, Gaurav Pandey, Ravi Iyengar, Marc R. Birtwistle
containing the Akt KTR. Transformants were miniprepped with the
PureYield Plasmid Miniprep System
(
Promega
A1223
) and Sanger sequence verified using GeneWiz. Akt KTR expression vector was generated by performing a
(12)
Single-Molecule Studies on the Motion and Force Generation of the Kinesin-3 Motor KIF1A
Optical Tweezers
October 21, 2022
Lu Rao, Arne Gennerich
room temperature. A temperature-controlled shaker.
PureYield™ plasmid miniprep system
(
Promega
, #
A1223
). 2.2
Mutagenesis
Q5® site-directed mutagenesis Kit
(
New England Biolabs
, #
E0554S
). Primers for
(13)
Generation of a bovine cell line for gene engineering using an HIV-1-based lentiviral vector
Scientific Reports
October 18, 2022
Nanami Morizako, Erika P. Butlertanaka, Yuri L. Tanaka, Honoka Shibata, Tamaki Okabayashi, Hirohisa Mekata, Akatsuki Saito
(
NEB
, Cat#
C2992H
). The plasmids were extracted with
PureYield Plasmid Miniprep System
(
Promega
, Cat#
A1222
). The sequences of bovine TRIM5 mRNA (Accession# LC727630) and
human
TRIM5α mRNA (Accession# LC727631)
(14)
Intrachromosomal Looping and Histone K27 Methylation Coordinately Regulates the lncRNA H19-Fetal Mitogen IGF2 Imprinting Cluster in the Decidual Microenvironment of Early Pregnancy
Cells
October 5, 2022
Xue Wen, Qi Zhang, Lei Zhou, Zhaozhi Li, Xue Wei, Wang Yang, Jiaomei Zhang, Hui Li, Zijun Xu, Xueling Cui,
et al.
transformed into TOP10. Plasmid DNA was collected by Wizard® Plasmid DNA Purification kit (
Promega
,
A1223
, MO, USA) and sequenced. 4.9.
Chromosome
Conformation Capture (3C) Furthermore, 3C
assays
were performed
(15)
De novo sphingolipid biosynthesis necessitates detoxification in cancer cells
Cell Reports
September 27, 2022
Meghan E. Spears, Namgyu Lee, Sunyoung Hwang, Sung Jin Park, Anne E. Carlisle, Rui Li, Mihir B. Doshi, Aaron M. Armando, Jenny Gao, Karl Simin,
et al.
Cells (
Agilent
#
200315
) and DNA was miniprepped with the
PureYield Plasmid Miniprep System
(
Promega
#
A1222
) for lentiviral
transfection
. Lentivirus production and
CRISPR
gene KO in cell lines
Lentiviruses
(16)
Transcriptomic plasticity of the hypothalamic osmoregulatory control centre of the Arabian dromedary camel
Communications Biology
September 23, 2022
Panjiao Lin et al.
harbouring the inserts of interest were harvested using PureYieldTM
Plasmid Miniprep
System Kit (
Promega
,
A1222
) and sequenced by Eurofins Genomics using the Sanger dideoxy
sequencing
method. In order to predict
(17)
Protocol to study sufficiency of cis-regulatory elements in mouse embryonic stem cells using a CRISPR-mediated knockin approach
STAR Protocols
September 16, 2022
Tomas Pachano, Alvaro Rada-Iglesias
28506
FuGENE HD Transfection Reagent
,
Promega
,Cat:
E2312
PureYield Plasmid Miniprep System
,
Promega
,Cat:
A1222
Q5® High-Fidelity 2× Master Mix,
New England Biolabs
,Cat:
M0492L
GoTaq polymerase,
Promega
,Cat:
M7123
PureLink™ Genomic DNA Mini Kit
(18)
The deubiquitinase USP7 promotes HNSCC progression via deubiquitinating and stabilizing TAZ
Cell Death & Disease
August 5, 2022
Jin Li, Yibin Dai, Han Ge, Songsong Guo, Wei Zhang, Yanling Wang, Laikui Liu, Jie Cheng, Hongbing Jiang
which contains eight synthetic TEADs binding sites (5′-ACATTCCA-3′). Dual-Luciferase reporter system (
A1222
,
Promega
, USA) was used for detecting luciferase reporter activity. After
transfection
with Myc-USP7-WT/C223S
(19)
Rbbp4 loss disrupts neural progenitor cell cycle regulation independent of Rb and leads to Tp53 acetylation and apoptosis
Developmental Dynamics
August 1, 2022
Laura E. Schultzâ€Rogers, Michelle L. Thayer, Sekhar Kambakam, Wesley A. Wierson, Jordan A. Helmer, Mark D. Wishman, Kristen A. Wall, Jessica L. Greig, Jaimie L. Forsman, Kavya Puchhalapalli,
et al.
vector pT3TS. A 1 μg linearized vector was purified with the
PureYield Plasmid Miniprep System
(
Promega
,
A1223
) and used as template for
in vitro
synthesis of capped mRNA with the Ambion mMessage Machine T3 Transcription
(20)
Cre/lox regulated conditional rescue and inactivation with zebrafish UFlip alleles generated by CRISPR-Cas9 targeted integration
eLife
June 17, 2022
Fang Liu, Sekhar Kambakam, Maira P Almeida, Zhitao Ming, Jordan M Welker, Wesley A Wierson, Laura E Schultz-Rogers, Stephen C Ekker, Karl J Clark, Jeffrey J Essner,
et al.
R0145S
). One μg linearized vector was purified with the
PureYield Plasmid Miniprep System
(
Promega
,
A1223
) and used as template for
in vitro
synthesis of capped mRNA with the Ambion mMessage Machine T3 Transcription
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Reviews
Write a Review
(1)
3/25/2016
Transfection Quality Plasmid DNA in Shortest Time
Sivaraman Natarajan
Our lab work is mostly focused on lineage tracing of cancer cells. We use Promega’s mini prep kit for various plasmid DNA preparations for cloning as well as transfection. This Procedure is very ...
Read Review
(1)
1/20/2015
Quick and pure miniprep kit for plasmid DNA
CHING-SHYI WU
Preparation of miniprep plasmid DNA is very common in most Bio-related labs. The purified DNA is used for cloning, DNA sequencing, PCR, and other processes. The PureYield miniprep kit from Promega is ...
Read Review
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PureYield Plasmid Miniprep System from Promega
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