PureYield Plasmid Miniprep System from Promega

Product Specs
ItemPureYield Plasmid Miniprep System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA1223
Time> 10 minutes
Volume
- If higher yields are desired-1.5–3.0ml of culture
- low-copynumber plasmids- 3ml culture volumes are recommended
Yieldpurify 1.5–7.5 µg of plasmid DNA
Quantity100 preps
Methodsilica membrane column
SourceBacterial
Nucleic Acid TypePlasmid DNA
Applicationspurification
Sample TypeBacterial culture

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Myosin IIA motor regulates attaching-effacing bacteria interactions with intestinal epitheliumGut MicrobesDecember 31, 2026Nayden G. Naydenov, Atif Zafar, Susana Lechuga, Ajay Zalavadia, Armando Marino-Melendez, John A. Hammer, Velia M. Fowler, Christine McDonald, Kenneth G. Campellone, Andrei I. Ivanovisolated from individual colonies using the PureYield Miniprep System (Promega, Madison, WI, cat. #A1222). The presence of the desired mutations was verified by DNA sequencing. For transfection, Cos-7 cells

(2) Workflow for Crystallographic Fragment Screening by Crystal Soaking for Protein Targets: A Case Study on Thioredoxin Glutathione Reductase from Schistosoma mansoniBIO-PROTOCOLApril 20, 2026Lauro R. S. Neto, Bogar O. Montoya, José Brandão-Neto, Kelly Parker, Frank Von Delft, Nicholas Furnham, Raymond J. Owens, Floriano P. Silva-Jr(Sigma-Aldrich, catalog number: P8849) 48. PureYieldTM miniPlasmid Miniprep System (Promega, catalog number: A1223) 49. SG1 Crystallization Screen kit (Molecular Dimensions, catalog number: MD1-88) 50. SigmaMarker low-range

(3) The role of the VirB ligand CTP in the molecular mechanism of transcriptional anti-silencing in Shigella flexneriNucleic Acids ResearchFebruary 27, 2026Taylor M Gerson, Monika M A Karney, Helen J Wingprepared by isolating from DH10B cells using the Promega Pure Yield Plasmid Miniprep System (Promega #A1223). To prepare the DNA stock, 3 ml of culture was processed according to the manufacturer’s instructions

(4) Endogenous CD5L controls the metabolic and inflammatory state of human macrophagesFrontiers in ImmunologyJanuary 22, 2026Kashif Rasheed, Neda Nejati Moharrami, Erlend Bjørkøy Tande, Terje Espevik, Maria Yurchenko, Victor BoyartchukT4 DNA ligase reaction buffer (B0202) were from New England Biolabs, Ipswich, MA, USA. The PureYield Plasmid Miniprep System (A1222) was from Promega. Cells THP-1 human monocytic cell line (ATCC, TIB-202) was maintained in RPMI 1640

(5) HLA ‐Knockout: Enabling Allele‐Specific Knockout of HLA Class I Genes for Immunogenic EngineeringHLAJanuary 21, 2026Connor Mattivi, Shiyu Wang, Longtao Ji, Qian Xiao, Jian Cao(Thermo Fisher C737303) and amplified using the Promega PureYield Plasmid Miniprep System (Promega A1222). All constructs were verified by Sanger sequencing (Azenta Life Sciences). Guide sequences used are:

(6) De novo cysteine biosynthesis in Pseudomonas aeruginosa: Characterization of the two main cysteine synthase isoformsiScienceJanuary 16, 2026Rebecca Martedì, Jole Maria Lucia D’Angelo, Giulia Sassi, Marialaura Marchetti, Sarah Hijazi, Riccardo Percudani, Stefano Bettati, Barbara Campanini, Emanuela Frangipanipreps",Promega Italia Srl,Cat# A9281 "PureYield(TM) Plasmid Miniprep System, 100 preps",Promega Italia Srl,Cat# A1223 H2S/SULF calibration kit,Unisense A/S,Cat# CALKIT-H2S; Lot# 1.06 "Wizard(R) SV Gel and PCR Clean-up

(7) Investigating and correcting a rare pathogenic mutation in GDF11Human Genetics and Genomics AdvancesJanuary 15, 2026Sean T. Congdon, John Bennett, Rhoddean Opinya, Amara R. Agosto, Olivia Dossias, Christopher Kokko, Aime A. Levesque, Andrew O. Koob, Adam C. Silver, Cindy A. Thomas-Charles45567). Plasmid DNA was purified using the Promega PureYield Miniprep System (Promega, catalog no. A1222). Transformations were performed using New England Biolabs (NEB) 5-α Competent Escherichia coli (NEB,

(8) Functional Characterization of Glucokinase Variants to Aid Clinical Interpretation of Monogenic DiabetesInternational Journal of Molecular SciencesDecember 23, 2025Varsha Rajesh, Dora Evelyn Ibarra, Jing Yang, Haichen Zhang, Amy Barrett, Eleanor G. Kaplan, Amit Kumthekar, Fanny Sunden, Han Sun, Ananta Addala, et al.plasmid extraction (using Promega Pure Yield Plasmid Miniprep Kit, Promega, Madison, WI, USA, Cat: A1223). The region containing GCK was amplified, and the mutation introduced was validated by Sanger sequencing

(9) Origin and evolution of key enzymes in the anammox pathway revisitedGenome Biol EvolDecember 18, 2025Emil Hägglund, Alejandro Jiménez-González, Erik Hagström, Patrik Björkholm, Lionel Guy, Siv G E Anderssonresistant colonies were isolated and the plasmids were purified using PureYield mini prep kit (Promega, A1223) according to standard protocols. All plasmids were sequenced by Oxford nanopore technology at a minimum

(10) Loss of heterozygosity exposes germline mutations in complex I and drives Warburg metabolism in oncocytic carcinoma of the thyroidbioRxivDecember 9, 2025Celia de la Calle Arregui, Anderson R. Frank, Kelvin Mun, Jiwoong Kim, Kuntal Majmudar, Justin A. Bishop, Cheryl Lewis, Yang Xie, David G. McFaddengrown under ampicillin selection (100 μg/mL). Plasmid DNA was isolated using the Promega Miniprep Kit (#A1222). Lentiviruses encoding sgRNAs were produced as described above. TPC-1 cells were seeded at 4 × 105

(11) MiR-219a-Robo1 axis acts as a conserved switch for Slit-mediated commissural axon guidanceiScienceNovember 27, 2025Bhakti Khot, Harindi Suriyaarachchi, Tanushree Majumder, Anagaa Nathan, Q. Richard Lu, Guofa LiuDual-Luciferase® Reporter Assay System,Promega,Cat#E1910 PureLinkTM RNA Mini Kit,ThermoFisher,Cat#12183018A PureYieldTM Plasmid Miniprep Kit,Promega,Cat#A1222 HiSpeed Plasmid Midi Kit,Qiagen,Cat#12643 AccuScript High Fidelity 1st Strand cDNA Synthesis Kit,Agilent Technologies

(12) Development of a Porcine Cell Line Stably Expressing Ephrin‐B2 for Nipah Virus Research and Diagnostic TestingMicrobiology and ImmunologyNovember 17, 2025Hui Zhang, Akatsuki SaitoC2992H) and extracted using the PureYield Plasmid Miniprep System (Promega, Madison, WI, USA, Cat# A1222). The plasmid sequence was verified using a SupreDye v3.1 Cycle Sequencing Kit (M&S TechnoSystems, Osaka,

(13) CRISPRi-seq in Haemophilus influenzae reveals genome-wide and medium-specific growth determinantsPLOS PathogensOctober 31, 2025Celia Gil-Campillo, Johann Mignolet, Asier Domínguez-San Pedro, Beatriz Rapún-Araiz, Axel B. Janssen, Vincent de Bakker, Jan-Willem Veening, Junkal Garmendiawas selected for culturing and plasmid purification using PureYield Plasmid Miniprep System (Promega, A1222). Purified plasmids were used to amplify each sgRNA cassette by PCR with F_AarI-Hi0601up/2477 and R_AarI_Hi0601dn/2482

(14) miR-155-5p Silencing Does Not Alter BTLA Molecule Expression in CLL T Cells: Implications for Targeted ImmunotherapyBiomoleculesOctober 24, 2025Agata Kosmaczewska, Lidia Ciszak, Anna Andrzejczak, Anna Tomkiewicz, Anna Partyka, Zofia Rojek-Gajda, Irena Frydecka, Dariusz Wołowiec, Tomasz Wróbel, Agnieszka Bojarska-Junak, et al.Inc., Waltham, MA, USA). Plasmids were purified using the PureYield™ Plasmid Miniprep System (Cat. no. A1223, Promega, Madison, WI, USA). All constructs were verified by restriction enzyme digestion and/or PCR

(15) SARS-CoV-2 Nsp15 endoribonuclease subverts host defenses to enhance viral fitness in lung cellsJournal of VirologySeptember 23, 2025Xiang Chi, Xueying Liang, Kishore Vaddadi, Xiaoming Zhang, Chaitanya Gandikota, Sunil More, Lin Liu, Xufang Dengovernight. Colonies were picked and cultured, and plasmid DNA was extracted using miniprep (Promega A1222). Diagnostic digestion with KpnI-HF (NEB R3142S) and AgeI (NEB R3552S) confirmed the correct constructs

(16) MicroRNA-Responsive ON-OFF Hybrid mRNA Switch for Precise Protein Expression ControlMolecular Therapy Nucleic AcidsSeptember 9, 2025Kaito Masaki, Yoshihiko Fujita, Hirohide SaitoLB/ampicillin (Amp) media, plasmids were purified by the PureYield Plasmid Miniprep System (Promega, no. A1223). piRFP670-N1 was gift from V. Verkhusha (Addgene plasmid #45457; http://n2t.net/addgene:45457; RRID:Addgene_45457).44

(17) HIF-independent oxygen sensing via KDM6A regulates ferroptosisMolecular CellAugust 7, 2025Alexander M. Minikes, Pei Liu, Hua Wang, Jiachen Hu, Hanan Alawaseem, Yueming Li, Xuejun Jiangassays IscriptTM cDNA Synthesis Kit,Bio-Rad,Cat # 1708891 PureYieldTM Plasmid Miniprep System,Promega,Cat # A1222 CellTiter-Glo CellTiter-Gloa Luminescent Cell Viability Assay,Promega,Cat # G7572 IqTM SYBR,Bio-Rad,Cat

(18) Conserved Yet Divergent Smc5/6 Complex Degradation by Mammalian Hepatitis B Virus X ProteinsInt J Mol SciJuly 15, 2025Maya Shofa, Yuri V Fukushima, Akatsuki SaitoCat# C2992H) and isolated using the PureYield Plasmid Miniprep System (Promega, Madison, WI, USA, Cat# A1222). The sequences of all the plasmids were verified using a SupreDye v3.1 Cycle Sequencing Kit (M&S TechnoSystems,

(19) Indels allow antiviral proteins to evolve functional novelty inaccessible by missense mutationsCell GenomicsJune 11, 2025Jeannette L. Tenthorey, Serena del Banco, Ishrak Ramzan, Hayley Klingenberg, Chang Liu, Michael Emerman, Harmit S. Malikassays,Critical commercial assays ,, PureYield miniprep kit (transfection-grade DNA),Promega,Cat # A1223 Nucelobond Xtra midiprep kit (transfection-grade DNA),Takara,Cat # 740410.50 Cytofix/Cytoperm fixation/permeabilization

(20) Correlation of p53 oligomeric status and its subcellular localization in the presence of the AML-associated NPM mutantPLoS OneMay 7, 2025Aleš Holoubek, Dita Strachotová, Kateřina Wolfová, Petra Otevřelova, Sára Belejová, Pavla Röselová, Aleš Benda, Barbora Brodská, Petr Hermancompetent cells (ThermoFisher Scientific), then purified with the PureYield Plasmid Miniprep System (A1223, Promega). All introduced and mutated constructs were verified by sequencing. List of constructs can

Write a Review

(1)

3/25/2016Transfection Quality Plasmid DNA in Shortest Time Sivaraman NatarajanOur lab work is mostly focused on lineage tracing of cancer cells. We use Promega’s mini prep kit for various plasmid DNA preparations for cloning as well as transfection. This Procedure is very ... Read Review
(1)

1/20/2015Quick and pure miniprep kit for plasmid DNA CHING-SHYI WUPreparation of miniprep plasmid DNA is very common in most Bio-related labs. The purified DNA is used for cloning, DNA sequencing, PCR, and other processes. The PureYield miniprep kit from Promega is ... Read Review