PureYield Plasmid Midiprep System from Promega

Product Specs
ItemPureYield Plasmid Midiprep System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA2492
Quantity25 preps
Methodsilica-membrane column
SourceBacterial
Nucleic Acid TypePlasmid DNA
Applicationspurification
Sample TypeBacterial culture

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Identification of fidelity-determined residues of Porcine reproductive and respiratory syndrome virus through structural alignmentVirulenceDecember 31, 2026Xiang Gao, Junnan Zhang, Peng Gao, Xinna Ge, Yongning Zhang, Jun Han, Xin Guo, Lei Zhou, Hanchun Yangclones were verified by sequencing and prepared using the PureYield plasmid midiprep system (Promega, A2492). The sequenced infectious clone plasmids were transfected into MARC-145 cells using Lipofectamine™ LTX Reagent

(2) CRAGE-RB-PI-seq reveals transcriptional dynamics of plant-associated bacteria during root colonizationNature CommunicationsFebruary 21, 2026Tomoya Honda, Sora Yu, Dung Mai, Leo Baumgart, Emory M. Chan, Gyorgy Babnigg, Yasuo Yoshikunithe designed library. Plasmid DNA was then extracted from the colonies using a midiprep kit (Promega: A2492). The extracted plasmids were subsequently transformed into E. coli WM3064 competent cells to be used

(3) Foxi1 regulates multipotent mucociliary progenitors and ionocyte specification through transcriptional and epigenetic mechanismsPLoS BiolJanuary 5, 2026Sarah Bowden, Magdalena Maria Brislinger-Engelhardt, Mona Hansen, Aisha Andricek, Africa Temporal-Plo, Damian Weber, Sandra Hägele, Fabian Lorenz, Tim Litwin, Clemens Kreutz, et al.were cloned using primers listed in Table 3 and purified using the Pure Yield midiprep kit (Promega #A2492) and injected at 5 ng/μl. foxi1.S reporter construct cloning and experiments To generate the foxi1.S::gfp-utrophin

(4) Fragments of viral surface proteins modulate innate immune responses via formyl peptide receptorsiScienceAugust 15, 2025Heiko Heilmann, Lukas Busch, Celine Buchmann, Islam Mohamed, Adrian Theiß, Sabryna Junker, Stefan Lohse, Bernd Bufe,R&D systems,Cat#F9M00 ECL Prime Kit,GE Healthcare,Cat#28980926 PureYield Plasmid Midiprep System,Promega,Cat#A2495 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, HEK293T,ATCC,Cat#CRL-1573 HL-60,CLS,Cat#300209 ,, Recombinant

(5) qByte: An open-source isothermal fluorimeter for democratizing analysis of nucleic acids, proteins and cellsPLOS BiologyMay 28, 2025Francisco J. Quero, Guy Aidelberg, Hortense Vielfaure, Yann Huon Huon de Kermadec, Severine Cazaux, Amir Pandi, Ana Pascual-Garrigos, Anibal Arce, Samuel Sakyi, Urs Gaudenz, et al.E.coli Top10 in a 200 mL culture, the plasmid was extracted via PureYield Plasmid Midiprep System (A2492, Promega) and concentrated at 30 nM per reaction. The RNA trigger was prepared by in vitro transcription

(6) Ten years after introduction of NADC30-like strain in China: a novel chimeric porcine reproductive and respiratory syndrome vaccine candidateFront ImmunolMay 8, 2025Weixin Wu, Xinyu Fang, Yiyao Jiang, Jiameng Hu, Qiongqiong Zhou, Peng Gao, Yongning Zhang, Xinna Ge, Jun Han, Xin Guo, et al.transformation of the recombinant plasmid, screening, propagation and plasmid extraction (Promega, #A2492). The primers used to generate the chimeric plasmids are listed in Table 1 . Recovery of chimeric

(7) A homozygous human WNT11 variant is associated with laterality, heart and renal defectsDisease Models & MechanismsMay 1, 2025Henrike Berns, Damian Weber, Maximilian Haas, Zeineb Bakey, Magdalena Maria Brislinger-Engelhardt, Miriam Schmidts, Peter Walentekstored in 100% ethanol at −20°C until use. DNAs were purified using a PureYield Midiprep kit (Promega, A2492) and linearized before in vitro synthesis of anti-sense RNA probes using T7 or Sp6 polymerase (Promega,

(8) The role of major and minor structural proteins of porcine reproductive and respiratory syndrome virus in induction of protective immunityFrontiers in MicrobiologyMarch 19, 2025Dan Li, Laixu Zhu, Chenchen Cui, Zhenchun Wu, Pengkai Qing, Qiongqiong Zhou, Peng Gao, Yongning Zhang, Lei Zhou, Xinna Ge, et al.verification through DNA sequencing and were prepared using the PureYield plasmid miniprep system (Promega, A2492). Recovery of chimeric viruses The infectious cDNA clone plasmids were transfected into MARC-145 cells

(9) Loss-of-function mitochondrial DNA polymerase gamma variants cause vascular smooth muscle cells to secrete a diffusible mitogenic factorFrontiers in PhysiologyFebruary 17, 2025Samantha Rothwell, Irvin Ng, Sophia Shalchy-Tabrizi, Pola Kalinowski, Omnia M. Taha, Italia Paris, Angelica Baniqued, Lisa Lin, Michelle M. Mezei, Anna Lehman, et al.kit (E.Z.N.A.® Endo-Free Plasmid DNA Midi Kit Cat #D6915-03 or Promega PureYield Midiprep Cat # PR-A2492). To create POLG-expression constructs with self-cleaving nuclear-targeted EGPF, we cloned the POLG

(10) Protein binding in an mRNA 5′-UTR sterically hinders translationRNAFebruary 1, 2025Simon Felder, Irma M. Nelson, Breanne M. Hatfield, Kevin M. Weeksmedium supplemented with 100 µg/mL of ampicillin for 24 h, extracted, purified (PureYield Midiprep; A2495, Promega), and stored at −20°C. Plasmid quality was confirmed by linearization and agarose gel electrophoresis

(11) Protocol for detecting genomic insulators in Drosophila using insulator-seq, a massively parallel reporter assaySTAR ProtocolsDecember 20, 2024Anastasiia Tonelli, Pascal Cousin, Maria Cristina Gambettaassays,Critical commercial assays,Critical commercial assays ,, PureYield plasmid midiprep system,Promega,A2495 MinElute PCR purification kit,QIAGEN,28004 NucleoSpin gel and PCR clean-up,Macherey-Nagel,740609.50 QIAGEN

(12) Activity-dependent COX-2 proteolysis modulates aerobic respiration and proliferation in a prostaglandin-independent manneriScienceDecember 20, 2024Liat Hagit Hartal-Benishay, Sharon Tal, Amal Abd Elkader, Omar Ehsainieh, Ranin Srouji-Eid, Tali Lavy, Oded Kleifeld, Martin Mikl, Liza Barki-HarringtonQuikChange Lightning Site-Directed Mutagenesis Kit,Agilent,210519 PureYield™ Plasmid Midiprep System,Promega,A2492 Quick-RNA™ Miniprep Plus Kit,Zymo,ZR-R1058 NEBNext Ultra RNA library Prep kit for Illumina,Thermo

(13) Foxi1 regulates multiple steps of mucociliary development and ionocyte specification through transcriptional and epigenetic mechanismsbioRxivNovember 7, 2024Sarah Bowden, Magdalena Maria Brislinger-Engelhardt, Mona Hansen, Africa Temporal-Plo, Damian Weber, Sandra Hägele, Fabian Lorenz, Tim Litwin, Clemens Kreutz, Peter Walentekfoxi1Δ1::gfp-utrophin and a-tub::mscarletI plasmids were purified using the Pure Yield midiprep kit (Promega #A2492) and injected at 5 ng/μl. Morpholino Sequences and doses: Full length foxi1 cloning (3’- 5’): foxi1.S

(14) Geosmin, a Food- and Water-Deteriorating Sesquiterpenoid and Ambivalent Semiochemical, Activates Evolutionary Conserved Receptor OR11A1Journal of Agricultural and Food ChemistryJuly 17, 2024Lena Ball, Tim Frey, Franziska Haag, Stephanie Frank, Sandra Hoffmann, Matthias Laska, Martin Steinhaus, Klaus Neuhaus, Dietmar Krautwurstexpression plasmid IL-6-HaloTag-pFN210A and were purified using the PureYield Plasmid Midiprep System (no. A2495; Promega, Madison, USA). The evolutionary history of the OR11A1 orthologs was inferred by employing

(15) Human CRB1 and CRB2 form homo- and heteromeric protein complexes in the retinaLife science allianceJune 1, 2024Stehle IF, Imventarza JA, Woerz F, Hoffmann F, Boldt K, Beyer T, Quinn PM, Ueffing M.Plasmid DNA preparation was performed using the column-based PureYieldTM Plasmid Midi Preparation Kit (A2495; Promega), and the sequence was confirmed by Sanger Sequencing (Microsynth). Site-directed mutagenesis To

(16) α-catenin middle- and actin-binding domain unfolding mutants differentially impact epithelial strength and sheet migrationMolecular Biology of the CellMay 1, 2024Jeanne M. Quinn, Yuou Wang, Megan Wood, Annette S. Flozak, Phuong M. Le, Alex Yemelyanov, Patrick W. Oakes, Cara J. GottardiRNAiMax,Invitrogen,Catalogue#13778-150,Catalogue#13778-150 PureYield Plasmid Midiprep System,Promega,Catalogue#A2492,Catalogue#A2492 Mitomycin,Sigma-Aldrich,Catalogue#M5353,Catalogue#M5353 Experimental Models: Organisms/Strains,Experimental Models

(17) Petrol Note in Riesling – 1,1,6-Trimethyl-1,2-dihydronaphthalene (TDN) Selectively Activates Human Odorant Receptor OR8H1Journal of Agricultural and Food ChemistryMarch 6, 2024Franziska Haag, Tim Frey, Lena Ball, Sandra Hoffmann, Dietmar Krautwurstinto the pFN210A expression plasmid and were purified using the PureYield Plasmid Midiprep System (#A2495, Promega, Madison, USA). Cell Culture We used HEK-293 cells42 (RRID:CVCL_0045), a human embryonic

(18) Interactome Analysis Reveals a Link of the Novel ALMS1-CEP70 Complex to Centrosomal ClustersMolecular & cellular proteomics : MCPJanuary 1, 2024Woerz F, Hoffmann F, Antony S, Bolz S, Jarboui MA, Junger K, Klose F, Stehle IF, Boldt K, Ueffing M, et al.Roth) agar plates. The final vector DNA was isolated using PureYield Plasmid Midi Preparation Kit (A2495, Promega) and sent for Sanger Sequencing (Eurofins, MicroSynth). Gateway Cloning pEBTetBI-CLIP-Cep70

(19) Occludin Regulates HIV-1 Infection by Modulation of the Interferon Stimulated OAS Gene FamilyMolecular neurobiologySeptember 1, 2023Torices S, Teglas T, Naranjo O, Fattakhov N, Frydlova K, Cabrera R, Osborne OM, Sun E, Kluttz A, Toborek M.Cat# C737303) and isolated using PureYield Plasmid midi-prep system (Promega, Madison, WI, USA, Cat# A2492). Viral stocks were created as described [15]. Briefly, a total of 50 μg of proviral plasmid was transfected

(20) Protocol to establish an oviduct epithelial cell line derived from Gallus gallus using Percoll for in vitro validation of recombinant proteinsSTAR ProtocAugust 4, 2023Chiron Loubser, Natalya V. Nikitinacabinet,Esco,Cat#2120759 Econo-Pac Chromatography Columns,Bio-Rad,Cat#7321010 PureYield Plasmid Midiprep System,Promega,Cat#A2492 10K MWCO Vivaspin 6 concentrator column,Sartorius,Cat#VS.0601 VWR Instructor’s Dissecting Kit,VWR,Cat#76457-530 Cohan-Vannas

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5/18/2016Large High Quality Plasmid DNA Kit in Nominal Cost Sivaraman NatarajanOur lab regularly uses promega midi prep kit for large scale plasmid isolation from 100-200ml bacterial culture for transfection. We observed the quality of plamid DNA good enough for transfection. We... Read Review

PureYield™ Plasmid Midiprep System From Promega
Many protein expression procedures require large amounts of pure plasmid DNA. Over the years, a ... Read More
PureYield™ Plasmid Midiprep System from Promega
In addition to being very simple to use, all reagents can be stored at room temperature. So, no more... Read More