ImProm-II Reverse Transcriptase from Promega

Product Specs
ItemImProm-II Reverse Transcriptase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3802
Quantity100 reactions

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(41) Blocking nuclear export of HSPA8 after heat shock stress severely alters cell survivalScientific ReportsDecember 1, 2018Fengjuan Wang, Srinivasa Reddy Bonam, Nicolas Schall, Lauriane Kuhn, Philippe Hammann, Olivier Chaloin, Jean-Baptiste Madinier, Jean-Paul Briand, Nicolas Page, Sylviane Mullerwas synthetized from 200 ng of RNA using ImProm-II Reverse Transcriptase and oligo(dT)15 (Promega, A3802 and C1101). Ten ng of cDNA were then amplified using Taqman Real-Time PCR Master Mixes (ThermoFisher

(42) In Planta Preliminary Screening of ER Glycoprotein Folding Quality Control (ERQC) ModulatorsInternational Journal of Molecular SciencesJuly 23, 2018Lucia Marti, Andrea Lia, Ida-Barbara Reca, Pietro Roversi, Angelo Santino, Nicole Zitzmann

(43) CCN5 knockout mice exhibit lipotoxic cardiomyopathy with mild obesity and diabetesPloS oneJanuary 1, 2018Kim J, Joo S, Eom GH, Lee SH, Lee MA, Lee M, Kim KW, Kim DH, Kook H, Kwak TH, et al.QIAGEN). Total cDNA was obtained by reverse transcription using Improm-2 reverse transcriptase (Promega; A3802) with oligo-dT primers. Real-time PCR was performed using the TaKaRa Real Time System, according to

(44) Cabbage (Brassica oleracea var. capitata) Protects against H(2)O(2)-Induced Oxidative Stress by Preventing Mitochondrial Dysfunction in H9c2 CardiomyoblastsEvidence-based complementary and alternative medicine : eCAMJanuary 1, 2018Yang DK.from each group of cells was reverse transcribed into cDNA using ImProm II reverse transcriptase (Cat. No. A3802, Promega Co., Madison, WI, USA) with oligo-dT priming. qRT-PCR was conducted using a TaKaRa Thermal

(45) The quest for epigenetic regulation underlying unisexual flower development in Cucumis meloEpigenetics & ChromatinDecember 1, 2017David Latrasse et al.analyses, first-strand cDNA was synthesized from 1 μg of total RNA using Improm-II reverse transcriptase (A3802, Promega) according to the manufacturer’s instructions. 1/25th of the synthesized cDNA was mixed with

(46) The Arabidopsis SWI/SNF protein BAF60 mediates seedling growth control by modulating DNA accessibilityGenome BiologyDecember 1, 2017Teddy Jégu et al.instructions. First strand cDNA was synthesized from 2 μg of total RNAs using Improm-II reverse transcriptase (A3802, Promega) according to the manufacturer’s instructions. We mixed 1/25th of the synthesized cDNA with

(47) MAPK-triggered chromatin reprogramming by histone deacetylase in plant innate immunityGenome BiologyDecember 1, 2017David Latrasse et al.instructions. First strand cDNA was synthesized from 2 μg of total RNA using Improm-II reverse transcriptase (A3802, Promega) according to the manufacturer’s instructions. of the synthesized cDNA, 1/25th was mixed with

(48) Differential TLR activation of murine mesenchymal stem cells generates distinct immunomodulatory effects in EAEStem Cell Research & TherapyDecember 1, 2016Ana María Vega-Letter, Mónica Kurte, Catalina Fernández-O’Ryan, Melanie Gauthier-Abeliuk, Patricia Fuenzalida, Ivón Moya-Uribe, Claudia Altamirano, Fernando Figueroa, Carlos Irarrázabal, Flavio Carrión(Thermo Scientific), and cDNA was synthesized from 2 μg of RNA using a reverse transcription protocol (Improm II-RT, A3802; Promega). Quantitative real-time PCR (RT-qPCR) was performed in a Stratagene MX3000P thermocycler (Agilent

(49) Role of the Polymerase sub-unit DPB2 in DNA replication, cell cycle regulation and DNA damage response in ArabidopsisNucleic Acids ResearchMay 18, 2016José Antonio Pedroza-Garcia et al.instructions. First strand cDNA was synthesized from 2μg of total RNA using Improm-II reverse transcriptase (A3802, Promega) according to the manufacturer's instructions. 1/25th of the synthesized cDNA was mixed with

(50) mTORC1-mediated inhibition of polycystin-1 expression drives renal cyst formation in tuberous sclerosis complexNature communicationsMarch 2, 2016Pema M, Drusian L, Chiaravalli M, Castelli M, Yao Q, Ricciardi S, Somlo S, Qian F, Biffo S, Boletta A.extracted from kidneys, we used Oligo(dt)15 primers (#C110B) and ImProm-II Reverse Transcriptase (#A3802) from Promega. We performed quantitave real-time PCR analysis on duplicate using SYBR Green I master

(51) A SWI/SNF Chromatin Remodelling Protein Controls Cytokinin Production through the Regulation of Chromatin ArchitecturePLOS ONEOctober 12, 2015Teddy Jégu et al.instructions. First strand cDNA was synthesized from 2μg of total RNA using Improm-II reverse transcriptase (A3802, Promega) according to the manufacturer’s instructions. 1/25th of the synthesized cDNA was mixed with

(52) Modulation of deregulated chaperone-mediated autophagy by a phosphopeptideAutophagyMarch 4, 2015Christophe Macri, Fengjuan Wang, Inmaculada Tasset, Nicolas Schall, Nicolas Page, Jean-Paul Briand, Ana Maria Cuervo, Sylviane Mullerwas synthetized from 200 ng of RNA using ImProm-II Reverse Transcriptase and oligo(dT)15 (Promega, A3802 and C1101, respectively). Ten ng of newly cDNA templates were then amplified using RT2 SYBR Green Rox

(53) SET7/9 Methylation of the Pluripotency Factor LIN28A Is a Nucleolar Localization Mechanism that Blocks let-7 Biogenesis in Human ESCsCell Stem CellDecember 4, 2014Seung-Kyoon Kim et al.microscope (Olympus, Japan). Quantitative RT-PCR RNA samples were transcribed with the Improm Kit (A3802, Promega, Madison, WI) according to the manufacturer's guidelines. Real-time PCR analysis was performed

(54) Dual function of MIPS1 as a metabolic enzyme and transcriptional regulatorNucleic acids researchMarch 1, 2013David Latrasse, Teddy Jégu, Pin-Hong Meng, Christelle Mazubert, Elodie Hudik, Marianne Delarue, Céline Charon, Martin Crespi, Heribert Hirt, Cécile Raynaud, Catherine Bergounioux, Moussa Benhamedinstructions. First-strand cDNA was synthesized from 2 μg of total RNA using Improm-II reverse transcriptase (A3802, Promega) according to the manufacturer’s instructions. 1/25th of the synthesized cDNA was mixed with

(55) Conserved regulators of the cell separation process in SchizosaccharomycesFungal Genetics and BiologyMarch 1, 2012Anita Balazs, Gyula Batta, Ida Miklos, Lajos Acs-Szabo, Carlos R. Vazquez de Aldana, Matthias Sipiczki1987). cDNA synthesis was carried out with the ImProm-II 209 Reverse Transcriptase system (Promega, A3802) according to the manufacturer’s instruction. 210 0.5 μg RNA was used as template. For the RT-PCR

(56) Multiplexed, Direct miRNA Quantification from Cell Lysates without RNA IsolationProtocol ExchangeJanuary 4, 2011Heng-Phon Too, Qing 'En Lim, Guoqiang Wan, Yoon Khei Ho, Heng-Phon TooAlternatively, reconstitute from basic reagents) • Reverse Transcriptase kit \(ImProm-II; Promega, cat. no. A3802 or similar.) \! Critical: Other reverse transcription enzymes can be used, but the permissible lysis

(57) HDAC4 inhibits the transcriptional activation of mda-7/IL-24 induced by Sp1Cellular & Molecular ImmunologyMay 1, 2010Lina Pan, Hong Pan, Hao Jiang, Juan Du, Xiuli Wang, Baiqu Huang, Jun LuReagent manual and was reverse transcribed into cDNA using the ImProm-II Reverse Transcription System (A3802) supplied by Promega. The resulting cDNA was diluted fivefold with RNase-free water. Quantitative real-time PCR

(58) Metabolic preconditioning of donor organs: Defatting fatty livers by normothermic perfusion ex vivoMetabolic EngineeringJuly 1, 2009Deepak Nagrath, Hongzhi Xu, Yoko Tanimura, Rongjun Zuo, François Berthiaume, Marco Avila, Rubin Yarmush, Martin L. YarmushCA). Reverse transcription was conducted using the ImProm-II™ Reverse Transcription System (catalog # A3802, Promega Corporation, Madison, WI). First strand cDNA was amplified with the gene-specific primers shown

(59) Specificity of commercial anti-spectrin antibody in the study of fungal and Oomycete spectrin: Cross-reaction with proteins other than spectrinFungal Genetics and BiologyFebruary 13, 2008Marta Cotado-Sampayo, Pilar Okenve Ramos, Rubén Ortega Perez, Mukti Ojha, François Barja

(60) Engagement of PSGL-1 upregulates CSF-1 transcription via a mechanism that may involve SykCellular immunologySeptember 1, 2005Ba XQ, Chen CX, Xu T, Cui LL, Gao YG, Zeng XL.

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