ImProm-II Reverse Transcriptase from Promega

Product Specs
ItemImProm-II Reverse Transcriptase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3802
Quantity100 reactions

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(21) Role of laminin and cognate receptors in cholangiocarcinoma cell migrationCell adhesion & migrationDecember 1, 2021Islam K, Thummarati P, Kaewkong P, Sripa B, Suthiphongchai T.

(22) SPX-related genes regulate phosphorus homeostasis in the marine phytoplankton, Phaeodactylum tricornutumCommunications BiologyDecember 1, 2021Kaidian Zhang, Zhi Zhou, Jiashun Li, Jingtian Wang, Liying Yu, Senjie Lin

(23) CHD4 Conceals Aberrant CTCF-Binding Sites at TAD Interiors by Regulating Chromatin Accessibility in Mouse Embryonic Stem CellsMolecules and cellsNovember 30, 2021Han S, Lee H, Lee AJ, Kim SK, Jung I, Koh GY, Kim TK, Lee D.DNase-treated total RNA was applied for cDNA synthesis using an Improm-II Reverse transcription system (A3802; Promega, USA) according to the manufacturer’s protocol. For analysis of retrotransposon expression,

(24) Analysing the fitness cost of antibiotic resistance to identify targets for combination antimicrobialsNature MicrobiologyNovember 1, 2021Aviram Rasouly et al.total RNA to cDNA was performed with the ImProm reverse transcriptase system (Promega, cat. number: A3802). As a proxy for the rate of rRNA synthesis, the level of the short-lived 5’ end of the ribosomal RNA

(25) Hypermethylation of Mest promoter causes aberrant Wnt signaling in patients with Alzheimer's diseaseScientific reportsOctober 8, 2021Prasad R, Jung H, Tan A, Song Y, Moon S, Shaker MR, Sun W, Lee J, Ryu H, Lim HK, et al.TRIzol reagent (Invitrogen) and cDNA synthesis was performed using ImProm‐II™ Reverse Transcriptase (#A3802, Promega) according to the manufacturer's protocol. Quantitative PCR (qPCR) was carried out using CFX

(26) A New Role for Plastid Thioredoxins in Seed Physiology in Relation to Hormone RegulationInternational Journal of Molecular SciencesSeptember 27, 2021Guillaume Née, Gilles Châtel-Innocenti, Patrice Meimoun, Juliette Leymarie, Françoise Montrichard, Pascale Satour, Christophe Bailly, Emmanuelle Issakidis-Bourguetstep was performed with up to 480 ng total RNA, using the ImProm-II™ Reverse Transcription System #A3802 (Promega, Charbonnières, France) with oligo(dT) primer. Gene expression profiling was conducted by real-time

(27) The sugar-responsive circadian clock regulator bZIP63 modulates plant growthThe New phytologistSeptember 1, 2021Viana AJC, Matiolli CC, Newman DW, Vieira JGP, Duarte GT, Martins MCM, Gilbault E, Hotta CT, Caldana C, Vincentz M.1.5 µg total RNA (final volume 12.5 ml) was performed using ImProm II Reverse Transcriptase (cat. no. A3802; Promega) and oligo(dT)18 according to the manufacturer’s instructions. qRT‐PCR analyses were performed

(28) Stability of hepatitis B virus pregenomic RNA in plasma specimens under various temperatures and storage conditionsPeerJApril 14, 2021Pakkapon Rattanachaisit, Sirinporn Suksawatamnuay, Supachaya Sriphoosanaphan, Kessarin Thanapirom, Panarat Thaimai, Nipaporn Siripon, Sukanya Sittisomwong, Yong Poovorawan, Piyawat Komolmitinstructions. Isolated HBV RNA was reverse transcribed using ImProm-II Reverse Transcriptase (Cat No. A3802; Promega, Madison, WI, USA), with HBV-specific RT primers (5-ATTCTCAGACCGTAGCACACGACACCGAGATTGAGATCTTCTGCGAC-3)

(29) Insulin signaling and glucose metabolism in different hepatoma cell lines deviate from hepatocyte physiology toward a convergent aberrant phenotypeScientific ReportsDecember 1, 2020Angela Molinaro, Barbara Becattini, Giovanni Solinasguanidinium-thiocyanate method. The complementary DNA was prepared using reverse transcription kit (Promega A3802). qPCR was performed using SYBR green mix (Bio-Rad 172-5274) and the following primers: mouse G6P fwd

(30) Involvement of Arabidopsis BIG protein in cell death mediated by Myo-inositol homeostasisScientific ReportsDecember 1, 2020Quentin Bruggeman et al.(MACHEREY- NAGEL) and 2 μg of total RNA was subjected cDNA synthesis using Improm-II reverse transcriptase (A3802, PROMEGA). 1/25th of the synthesized cDNA was mixed with 100 nM solution of each primer and LightCycler 480 Sybr Green I master

(31) Cx3cr1CreERT2-driven Atg7 deletion in adult mice induces intestinal adhesionMolecular BrainDecember 1, 2020Younghwan Lee, Ji-Won Lee, Hyeri Nam, Seong-Woon YuKit (Qiagen, 73,404). cDNA was synthesized using the ImProm-II Reverse Transcriptase kit (Promega, A3802) and oligo-dT primers. TOPreal qPCR 2× PreMIX (SYBR Green with low ROX; Enzynomics, RT500M) containing

(32) Effects of dietary polyunsaturated fatty acid sources on expression of lipid-related genes in bovine milk somatic cellsScientific ReportsDecember 1, 2020Einar Vargas-Bello-Pérez, Nathaly Cancino-Padilla, Carolina Geldsetzer-Mendoza, María Sol Morales, Heidi Leskinen, Philip C. Garnsworthy, Juan J. Loor, Jaime RomeroMadison, USA), in the amount of 0.5 µL, and 1 µL ImProm-II Reverse Transcriptase (Promega, Cat. No. A3802, Madison, USA) was added. Following addition of transcription mix, the reaction was maintained at 25

(33) Identification of a Signaling Mechanism by Which the Microbiome Regulates Th17 Cell-Mediated Depressive-Like Behaviors in MiceThe American journal of psychiatryOctober 1, 2020Medina-Rodriguez EM, Madorma D, O'Connor G, Mason BL, Han D, Deo SK, Oppenheimer M, Nemeroff CB, Trivedi MH, Daunert S, et al.

(34) A mutation in POLR3E impairs antiviral immune response and RNA polymerase IIIProceedings of the National Academy of Sciences of the United States of AmericaSeptember 8, 2020Ramanathan A, Weintraub M, Orlovetskie N, Serruya R, Mani D, Marcu O, Stepensky P, Weisblum Y, Djian E, Shaag A, et al.Samples of 15 ng of RNA were used as templates for cDNA preparation using random primers (Promega, A3802). RT-PCR was performed using Sybr green assay (Roche). The POLR3E mRNA was measured at three different

(35) Comparative Effects of Graphene and Molybdenum Disulfide on Human Macrophage ToxicitySmall (Weinheim an der Bergstrasse, Germany)September 1, 2020Lin H, Ji DK, Lucherelli MA, Reina G, Ippolito S, Samorì P, Bianco A.manufacturer’s instructions then converted to cDNA using the ImProm-II Reverse Transcription Set (Promega, #A3802). Two hundred nanograms of cDNA per reaction was used for real-time PCR on a StepOne machine (Applied

(36) Effects of Dietary Vegetable Oils on Mammary Lipid-Related Genes in Holstein Dairy CowsAnimals (Basel)December 27, 2019Einar Vargas-Bello-Pérez, Carolina Geldsetzer-Mendoza, Nathaly Cancino-Padilla, María Sol Morales, Heidi Leskinen, Philip C. Garnsworthy, Juan J. Loor, Jaime RomeroMadison, WI, USA), in the amount of 0.5 µL, and 1 µL ImProm-II Reverse Transcriptase (Promega®, Cat. No. A3802, Madison, WI, USA) was added. Following addition of transcription mix, the reaction was maintained at

(37) Polycystin-1 Regulates Actomyosin Contraction and the Cellular Response to Extracellular StiffnessScientific reportsNovember 12, 2019Nigro EA, Distefano G, Chiaravalli M, Matafora V, Castelli M, Pesenti Gritti A, Bachi A, Boletta A.extracted RNA using Oligo(dt)15 primers (Promega, #C1101) and ImProm-II Reverse Transcriptase (Promega, #A3802). Quantitative Real Time PCR analysis was performed on technical duplicates using SYBR Green I master

(38) Landscape of ribosome-engaged transcript isoforms reveals extensive neuronal-cell-class-specific alternative splicing programsNature NeuroscienceOctober 1, 2019Elisabetta Furlanis, Lisa Traunmüller, Geoffrey Fucile, Peter Scheiffelefrom all samples using random primers (Promega, C118A) and ImProm-II™ Reverse Transcriptase (Promega, A3802). For evaluation of differential reporter processing in different cells and conditions, cDNA amounts

(39) Long-Term Effects of Dietary Olive Oil and Hydrogenated Vegetable Oil on Expression of Lipogenic Genes in Subcutaneous Adipose Tissue of Dairy CowsVeterinary SciencesSeptember 15, 2019Einar Vargas-Bello-Pérez, Massimo Bionaz, Pietro Sciarresi-Arechabala, Nathaly Cancino-Padilla, María Sol Morales, Jaime Romero, Heidi Leskinen, Philip C. Garnsworthy, Juan J. LoorMadison, WI, USA) with a volume of 0.5 µL, and 1 µL ImProm-II Reverse Transcriptase (Promega, Cat. No. A3802, Madison, WI, USA) was added. After adding the transcription mix, the reaction was incubated for 5 min

(40) A Sam68-dependent alternative splicing program shapes postsynaptic protein complexesThe European journal of neuroscienceJune 1, 2019Witte H, Schreiner D, Scheiffele P.reverse transcribed using random hexamers (Promega #C1181) and ImProm II reverse transcriptase (Promega #A3802). Illumina paired-end sequencing and data analysis RNA-Seq and alternative splicing analysis anlysis

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