Dead Cell Removal Kit from Miltenyi Biotec

(1) Enhanced endocrine–metabolic support and axonemal assembly in high-sperm-motility geese: insights from testicular cellular heterogeneity by scRNA-seqPoultry ScienceJune 1, 2026Hechuan Wang, Kexin Cong, Jiaxin Yin, Ke Jiang, Yunuo Liu, Xiaofang Ren, Ying Zhang, Shengjun LiuRhine-Westphalia, Germany) and centrifuged at 4°C (300 × g, 5 min). Dead cells were removed using a Dead Cell Removal Kit (Miltenyi Biotec, North Rhine-Westphalia, Germany) and passed through MS columns (Miltenyi Biotec, North

(2) Protocol for culture, drug treatment, and dissociation of patient-derived 3D ovarian cancer microspheroids for flow cytometrySTAR ProtocolsJune 1, 2026Mariam Haffa, Karolina Juhani, Greta Gudoityte, Rita Hutyra-Gram Ötvös, Emelie Wallin, Olli-Pekka Kallioniemi, Josefin Fernebro, Ulrika Joneborg, Brinton Seashore-Ludlowtubes,Merck,Cat#CLS3213 TrypLE™ Express Enzyme 1X,Thermo Fisher Scientific,Cat#11538856 Dead Cell Removal Kit,Miltenyi,Cat#130-090-101 LS columns,Miltenyi,Cat#130-042-401 QuadroMACS™ Separator,Miltenyi,Cat#130-090-976 96-well V-shape

(3) Macrophage‐Fibroblast Crosstalk Shapes Wound Repair Signaling In VitroFASEB JMay 18, 2026Daniela Enriquez‐Ochoa, Raji R. Nagalla, Yingzi Liu, Christian F. Guerrero‐Juarez, Hamza Atcha, Gordon Chang, Nancy A. Morales, Hannah J. Hooper, Arian D. Cano, Huy Eng Lim, et al.for 72 h. To digest the gels, collagenase (10 mg/mL, Fisher Scientific) was added for 10 min, and a Dead Cell Removal kit (Miltenyi Biotec) was used according to the manufacturer's instructions to deplete dead cells. RNA was

(4) Preliminary Analysis of the Proportion and Characteristics of Dual BCR B Cells in SLE Model Mice and Patients via scRNA-Seq Combined with scBCR-Seq TechnologyCellsMay 17, 2026Kai Quan, Hongxia Yang, Guangtian Tang, Ziwei Li, Hailin Zou, Jing Ma, Xinsheng YaoChina). Dead cell removal was performed according to the instructions of the MACS Dead Cell Removal Kit (130-090-101) (Miltenyi Biotec, Bergisch Gladbach, Germany). 100 μL of magnetic beads were added to the cell suspension,

(5) Single-cell RNA-sequencing of dermal fibroblasts demonstrates culture-induced changes and variable persistence of keloid disease featuresiScienceMay 15, 2026Amy Lock, Elena M. Drudi, Dasha Freydina, Brian M. Stramer, Franziska Denk, Tanya J. Shawvials were thawed and enriched for live cells using the MACS Dead Cell Removal Kit (Miltenyi Biotec, #130-090-101). To study the effects of matrix deposition on fibroblast phenotype, a protocol based on cell-derived

(6) Single-cell analysis of follicular fluid reveals dysregulation of ovulatory immune function in Polycystic Ovary Syndrome patients undergoing ovarian stimulation†Biology of ReproductionMay 13, 2026Andrea K Wegrzynowicz, Soma Banerjee, Emily Grimes, Riley Huddleston, Fernanda Leyva Leyva Jaimes, Laura G Cooney, Aleksandar K Stanicfor live cells by using a Dead Cell Removal Kit (Miltenyi Biotec, Bergisch Gladbach, Germany, cat# 130-090-101) with magnetic microbeads and Octomacs Magnetic separation columns (Miltenyi, cat # 130-042-109), following

(7) Treg-derived IFN-γ supports the differentiation of Th1-Treg in tumor immunity and autoimmunityFront ImmunolMay 12, 2026Ayumi Kuratani, Masaaki Okamoto, Sekar Arumsari, Takashi Kobayashi, Miwa Sasai, Masahiro Yamamototo lyse red blood cells, and dead cells were removed using the Dead Cell Removal Kit (Miltenyi, Cat# 130-090-101). The resulting single-cell suspensions were resuspended in the appropriate buffer for downstream experiments. To

(8) An Advanced Single-Cell RNA Sequencing (scRNA-seq) Protocol Utilizing Custom-Designed MultiplexingBio ProtocMay 5, 2026Feng Gao, Xujie Liu, Fan Sun, Yadong Xiao, Gutian Xiao, Zhaoxia Quenzyme D and enzyme A preparation before use. 2. Dead Cell Removal kit (Miltenyi Biotec, catalog number: 130-090-101); contains dead cell removal microbeads and 20× binding buffer stock solution 3. Red blood cell lysis solution (10

(9) Remodeling of self-assembled microvascular networks under long term flowbioRxivMay 1, 2026Marie Floryan, Elena Cambria, Adriana Blazeski, Mark F. Coughlin, Zhengpeng Wan, Giovanni Offeddu, Vinayak Vinayak, Aayush Kant, Vivek Shenoy, Roger D. Kammagitation. Cells from 3–4 devices were pooled for each replicate. Dead cells were removed using the Dead Cell Removal Kit and an OctoMACS Separator (Miltenyi Biotec), cells were incubated with CD31 MicroBeads (CD31 MicroBead

(10) Oral Streptococcus salivarius Couples Neutrophil IRGM1 Signaling to NET Formation and Colorectal Cancer MetastasisAdvanced ScienceMay 1, 2026Fengyi Liu, Yuan Wang, Hengxuan Cai, Qianwen Yue, Xueli Liu, Xuanwen Bao, Zeng Wang, Xuehan Li, Lai Wei, Zhihua Zhang, et al.staining and the SeekGene M002C fluorescence cell analyzer, with decisions to remove dead cells (Miltenyi, 130‐090‐101) or debris (Miltenyi, 130‐109‐398) based on viability and nuclear content.CD45+ cells were magnetically

(11) A Single-Cell Atlas of Uterine Carcinosarcoma from Diverse AncestriesbioRxivApril 29, 2026Santhilal Subhash, Marie-Thérèse Bammert, Brian Yueh, Timothy Chu, Mali Barbi, Aybuke Alici, Onur Eskiocak, Kadir A. Ozler, Aaron Nizam, Arielle Katcher, et al.(Corning, #431750) and dead cell removal was conducted using the Dead Cell Removal Kit (Miltenyi Biotec 130–090-101), following the manufacturer’s instructions. Samples averaged 80–90% viability with at least 1.5 million

(12) Single cell transcriptional evolution of myeloid leukemia of Down syndromeNat CommunApril 23, 2026Mi K. Trinh, Konstantin Schuschel, Hasan Issa, Rebecca Thomas, Conor Parks, Agnes Oszlanczi, Toochi Ogbonnah, Di Zhou, Lira Mamanova, Elena Prigmore, et al.through a 70μm cell strainer (Falcon) and washed with PBS. If necessary, live cell enrichment using a Dead Cell Removal kit (Miltenyi Biotec) and red blood cell removal using the eBioscience 10X RBC Lysis Buffer (Multi-species)

(13) Mapping breast cancer lineage in radiation and immunotherapy using the REMAP mousebioRxivApril 22, 2026Abigail C. Marshall, Jackie Vahey, Meisam Bagheri, Rachel Saxe, Jennifer Fields, Fred Kolling, Aaron McKennaSingle Cell Suspensions for Single Cell RNA Sequencing” using Miltenyi dead cell removal kit (Cat# 130-090-101), LS columns (Cat# 130-042-401), and QuadroMACS separator (Cat# 130-090-976). Final cell resuspensions

(14) Immunogenic tumor cell death and T-cell-derived IFN-γ elicit tumoricidal macrophages to potentiate OX40 immunotherapyCell Reports MedicineApril 21, 2026Yanqin Liu, Jiliang Zhao, Kailu Yang, Qiongqiong Ma, Dongping Zhang, Lili Xu, Zhaoyuan Zhang, Zhongqian Yin, Jingru Chen, Yi Wang, et al.221000 Mouse CD45(TIL)MicroBeads,Miltenyi Biotec,Cat# 130-110-618 Dead Cell Removal Kit,Miltenyi Biotec,Cat# 130-090-101 Chromium Single Cell 5′ Reagent Kits User Guide (v2 Chemistry Dual Index),10x Genomic,Cat# PN-1000263 SPARKeasy

(15) Single-Cell Transcriptomic Profiling of Ectopic ACTH-Secreting Pheochromocytoma Reveals the Chromaffin Cell Origin of Ectopic Hormone ProductionInt J Mol SciApril 18, 2026Xu Wang, Penghu Lian, Guoyang Zheng, Wenda Wang, Yang Zhao, Yushi Zhangthe cell pellet was collected after centrifugation. Add 100 μL of Dead Cell Removal MicroBeads (MACS 130-090-101; Miltenyi Biotec, Bergisch Gladbach, Germany), mix well, and incubate for 15 min at room temperature.

(16) A multimodal atlas for immunotherapeutic targeting of AML surface heterogeneityiScienceApril 17, 2026Matthew Ung, Julia Etchin, Amanda Halfond, Julia DiFazio, Yonina Keschner, Alyssa Pyclik, Anne Campbell, Ruijia Wang, Mariana Silva, Brikena Gjeci, et al.RPMI 1640 (1X) (Gibco™) + 10% fetal bovine serum (FBS) (Corning). Live BMMCs were enriched using the Dead Cell Removal Kit (Miltenyi Biotec) following the manufacturer’s protocol, passed through a magnetic column, and eluted

(17) Dedifferentiation-driven oncogenic stemness promotes tumor-sustaining adaptability in the intestinal epitheliumCell Death & DiseaseApril 17, 2026Kylee Zgeib, Thompson Hui, Simon Garcia, Zahra Hashemi, Shima Nejati, Amartya Singh, Atharva Inamdar, Crystal Lim, Dahlia Matouba, Christina Li, et al.Single-cell RNA sequencing Single cells prepared as described were washed in 0.1% BSA/PBS and processed with the Miltenyi Biotec Dead Cell Removal Kit. Live cells were pelleted, washed in 0.5% BSA/PBS, and stained with CD44-FITC (1:20) on ice for 20 min,

(18) Tumor reactivity assessment using clonal expression reveals tumor reactive CD8+ T cell heterogeneity across solid tumorsFront ImmunolApril 16, 2026David Monteiro, Jack Denebeim, Anne E. Dodson, Ashish Yeri, Mitali Ghose, Meghan Travers, Sophie Capobianco, Conor Calnan, Gustavo J. Martinez, Charles H. Yoon, et al.T cells from tumor starting material and from autologous co-cultures underwent dead cell removal (#130-090-101, Miltenyi Biotec) followed by T cell enrichment using CD3 microbeads (EasySep Human CD3 #17851, STEMCELL Technologies

(19) Histotripsy-Initiated Immune Response Synergizes with Chemotherapy in a Neuroblastoma Murine ModelCancers (Basel)April 15, 2026Natalia Antonides-Jensen, Muskan Singh, Yuqing Xue, Fernando Flores-Guzman, Lydia L. Wu, Samantha S. Yee, Jacky Gomez-Villa, Timothy L. Hall, Mark A. Applebaum, Kenneth B. Bader, et al.before proceeding with Dead Cell Removal Kit (Miltenyi Biotec, Bergisch Gladbach, Germany; cat. no. 130-090-101) per manufacturer’s instructions. Cell preparations with under 70% cell viability were excluded from

(20) Genome to single-cell characterization of the MAPK family in bighead carp (Hypophthalmichthys nobilis) spleen during Aeromonas hydrophila challengeFront ImmunolApril 13, 2026Yang Mao, Xue Yu, Wenjing Wang, Jin Peng, Minao Wang, Jianyun Zhou, Defeng LiBSA) (50). Viability was assessed by trypan blue (target ≥85%); a dead-cell removal kit (Miltenyi, 130-090-101) was used only if required. Cell concentration was adjusted to 700–1,200 cells/µL. 2.9 Single-cell

Write a Review

(1)

7/13/2020Kit Works Well at Removing Dead Cells Rebecca GuyerOur lab extracts cells from human tissues for downstream analysis. Due to our processing, many dead cells are in our single cell suspension and we need to remove as many as possible before doing any ... Read Review
(1)

6/4/2018Dead Cell Removal Kit Alex RayOur lab studies HIV immunology and the enrichment of live cells for downstream analysis is important for many of our protocols. This kit provides a reliable way to remove dead cells while maintaining ... Read Review
(1)

3/8/2018Dead Cell Removal Kit Lana JagannathanOur lab studies ways to enhance immune responses against HIV infection. As such we perform a lot of in vitro killing assays with different effector cells against HIV infected PBMC. One way to measure ... Read Review
(1)

7/8/2016Dead Cell Separation from Single Cell Preparation Sivaraman NatarajanWe prepare single cells from various tissues for our Genomic studies. We encountered lot of damaged and dead cells in the end from the preparation. This kit works works on MACS ... Read Review

Dead Cell Removal Kit (up to 100 separations)

Dead Cell Removal Kit from Miltenyi Biotec

Description

Miltenyi Biotec

Miltenyi Biotec

Citations (1286)

Reviews

Dead Cell Removal Kit from Miltenyi Biotec

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map