Dead Cell Removal Kit from Miltenyi Biotec

(1) Autophagy of OTUD5 destabilizes GPX4 to confer ferroptosis-dependent kidney injuryNat CommunDecember 18, 2023Li-Kai Chu, Xu Cao, Lin Wan, Qiang Diao, Yu Zhu, Yu Kan, Li-Li Ye, Yi-Ming Mao, Xing-Qiang Dong, Qian-Wei Xiong, et al.Trypan Blue (Gibco), and dead cells were removed using magnetic bead separation with a Dead Cell Removal Kit (130-090-101, Miltenyi Biotec) if the cell viability was <85%. At least 20,000 cells per sample were applied to a

(2) AMP-activated protein kinase is necessary for Treg cell functional adaptation to microenvironmental stressbioRxivDecember 1, 2023Manuel A. Torres Acosta, Nurbek Mambetsariev, Carla P Reyes Reyes Flores, Kathryn A. Helmin, Qianli Liu, Anthony M. Joudi, Luisa Morales-Nebreda, Jonathan Gurkan, Kathleen Cheng, Hiam Abdala-Valencia, et al.then subjected to debris removal (Miltenyi cat. # 130–109-398) and dead cell removal (Miltenyi cat. # 130–090-101) according to the kit manufacturer’s instructions. The debris and dead cell-depleted tumor homogenates

(3) Therapeutic targeting of tumor spheroids in a 3D microphysiological renal cell carcinoma-on-a-chip systemNeoplasiaDecember 1, 2023Chris P. Miller, Megan Fung, Carla A. Jaeger-Ruckstuhl, Yuexin Xu, Edus H. Warren, Shreeram Akilesh, Scott S. TykodiCellTracker (Ct) Green CMFDA (Thermo Fisher Scientific) in serum-free media for 30 minutes, subjected to the Dead Cell Removal Kit (Miltenyi Biotech), and formed into spheroids overnight using Aggrewell 400 plates (Stem Cell Technologies)

(4) Epicardioid single-cell genomics uncovers principles of human epicardium biology in heart development and diseaseNature biotechnologyDecember 1, 2023Meier AB, Zawada D, De Angelis MT, Martens LD, Santamaria G, Zengerle S, Nowak-Imialek M, Kornherr J, Zhang F, Tian Q, et al.15% cell death, dead cells were immediately depleted using a dead cell removal kit (Miltenyi Biotec, 130-090-101), according to the manufacturer’s instructions, before further processing. Cells from the same cell

(5) Liver cancer stem cell dissemination and metastasis: uncovering the role of NRCAM in hepatocellular carcinomaJournal of Experimental & Clinical Cancer ResearchNovember 22, 2023Lingyun Zhou, Linye He, Chang-Hai Liu, Huandi Qiu, Li Zheng, Klarke Michael Sample, Qin Wu, Jiaxin Li, Kunlin Xie, Javier Ampuero, et al.removal of erythrocytes (Miltenyi 130–094-183), debris (Miltenyi 130–109-398) and dead cells (Miltenyi 130–090-101). Finally, fresh cells were washed twice in the RPMI1640 and then resuspended at 1 × 106 cells per ml

(6) Analysis of intestinal epithelial cell responses to Cryptosporidium highlights the temporal effects of IFN-γ on parasite restrictionbioRxivNovember 21, 2023Ryan D. Pardy, Katelyn A. Walzer, Bethan A. Wallbank, Jessica H. Byerly, Keenan M. O’Dea, Ian S. Cohn, Breanne E. Haskins, Justin L. Roncaioli, Eleanor J. Smith, Gracyn Y. Buenconsejo, et al.sequencing. IEC/IEL single cell suspensions were prepared as above and dead cells were removed using the Dead Cell Removal Kit (Miltenyi Biotec) using LS columns (Miltenyi Biotec), per the manufacturer’s instructions. We note that

(7) A primate nigrostriatal atlas of neuronal vulnerability and resilience in a model of Parkinson’s diseaseNature CommunicationsNovember 18, 2023Lei Tang, Nana Xu, Mengyao Huang, Wei Yi, Xuan Sang, Mingting Shao, Ye Li, Zhao-zhe Hao, Ruifeng Liu, Yuhui Shen, et al.cell strainer. The debris and dead cells were removed using Myelin Removal Beads II (Miltenyi) and Dead Cell Removal Kit (Miltenyi) and then washed two times with 0.04% PBS-BSA. Cells were counted (Bio-Rad, TC20) after visual

(8) Targeting Translation and the Cell Cycle Inversely Affects CTC Metabolism but Not MetastasisCancers (Basel)November 2, 2023Tetiana Y. Bowley, Seth D. Merkley, Irina V. Lagutina, Mireya C. Ortiz, Margaret Lee, Bernard Tawfik, Dario Marchettiincubation at 4 °C. At 15 min into the 30 min incubation, 100 µL of Dead Cell Removal Beads (Miltenyi, Cat# 130-090-101) per 1 × 107 cells was added. Fractions were then subject to magnetic separation in LS Positive Selection

(9) A single‐cell transcriptomic atlas characterizes age‐related changes of murine cranial stem cell nichesAging CellNovember 1, 2023Bo Li, Jingya Li, Bingzhi Li, Takehito Ouchi, Longjiang Li, Yu Li, Zhihe Zhao4 min at 4°C and processed to remove dead cells using MACS® Dead Cell Removal Kit (Miltenyi Biotec, 130‐090‐101), followed by washing in 1× PBS (0.04% BSA) and centrifuging at 420 g for 4 min at 4°C. The cell pellet

(10) Identification of Cell-Cell Communications by Single-Cell RNA Sequencing in End Stage Renal Disease Provides New Insights into Immune Cell HeterogeneityJournal of Inflammation ResearchOctober 31, 2023Ruijing Zhang, Xinyan Liu, Yuehong Ma, Lijuan Cheng, Yi Ren, Rongshan Liapplied for PBMC isolation. Dead cells were removed using a Miltenyi ® Dead Cell Removal Kit (MACS, 130-090-101). Cell viability above 85% was required for scRNA sequencing using a Countess II Automated Cell Counter.

(11) Lactate oxidase nanocapsules boost T cell immunity and efficacy of cancer immunotherapyScience Translational MedicineOctober 11, 2023Zheng Cao, Duo Xu, Jeffrey Harding, Wenting Chen, Xiangsheng Liu, Zi Wang, Lan Wang, Tong Qi, Shilin Chen, Xinheng Guo, et al.red blood cells (RBC) were lysed with RBC lysis buffer (BioLegend) and dead cells were removed with the Dead Cell Removal Kit (Miltenyi Biotec). The cells were then counted and processed according to the protocol of the Chromium

(12) Unravelling the role of immune cells and FN1 in the recurrence and therapeutic process of skull base chordomaClinical and Translational MedicineOctober 2, 2023Xulei Huo, Sihan Ma, Can Wang, Lairong Song, Bohan Yao, Sipeng Zhu, Peiran Li, Liang Wang, Zhen Wu, Ke WangRed Blood Cell Lysis Solution from Miltenyi Biotec was utilised for the lysis of red blood cells, while the Dead Cell Removal Kit, also from Miltenyi Biotec, was employed to eradicate deceased cells. The cells were suspended in PBS

(13) Single-cell RNA sequencing reveals the heterogeneity and intercellular communication of hepatic stellate cells and macrophages during liver fibrosisMedComm (2020)October 1, 2023Cheng S, Zou Y, Zhang M, Bai S, Tao K, Wu J, Shi Y, Wu Y, Lu Y, He K, et al.hepatocytes. The NPCs supernatant suspension was collected and treated with a Dead Cell Removal Kit (130‐090‐101; Miltenyi Biotec) according to the manufacturer's instructions to remove dead cells. The resulting NPCs

(14) Spatially multicellular variability of intervertebral disc degeneration by comparative single-cell analysisCell proliferationOctober 1, 2023Lin P, Yan P, Zhu J, Huang S, Wang Z, Hu O, Jin H, Li Y, Zhang L, Zhao J, et al.Miltenyi Biotec) to remove the residual blood cells. We eliminated dead cells by labelling cells with Dead Cell Removal Kit (Miltenyi Biotec) and removing them over an LS column in the magnetic field of a MidiMACS™ Separator

(15) IL-7 receptor signaling drives human B-cell progenitor differentiation and expansionBloodSeptember 28, 2023Fabian M. P. Kaiser, Iga Janowska, Roberta Menafra, Melanie de Gier, Jakov Korzhenevich, Ingrid Pico-Knijnenburg, Indu Khatri, Ansgar Schulz, Taco W. Kuijpers, Arjan C. Lankester, et al.controls and the 2 patients with IL-7Rα–deficient SCID were thawed and dead cells were removed with the Dead Cell Removal kit (Miltenyi Biotec) according to the manufacturer’s instructions. Subsequently, non-B cells were depleted

(16) Oncolytic Virus-Driven Immune Remodeling Revealed in Mouse Medulloblastomas at Single Cell ResolutionMolecular Therapy - OncolyticsSeptember 21, 2023Jack Hedberg, Adam Studebaker, Luke Smith, Chun-Yu Chen, Jesse J. Westfall, Maren Cam, Amy Gross, Ilse Hernandez-Aguirre, Alexia Martin, Doyeon Kim, et al.a dead cell removal protocol was performed according to manufacturer instructions (Miltenyi Biotec, 130-090-101). scRNA-seq A Chromium Next GEM Single Cell 3′ v3.1 kit from 10× Genomics was used to carry out sequencing

(17) Isolation of Epithelial and Stromal Cells from Colon Tissues in Homeostasis and Under Inflammatory ConditionsBIO-PROTOCOLSeptember 20, 2023Clara Morral, Reem Ghinnagow, Tatiana Karakasheva, Yusen Zhou, Anusha Thadi, Ning Li, Benjamin Yoshor, Gloria E. Soto, Chia-Hui Chen, Daniel Aleynick, et al.solution (Gibco, catalog number: 15250061)Dead cell removal kit (Miltenyi Biotech, catalog number: 130-090-101) and necessary accessories (see Laboratory supplies)10× Chromium Next GEM Single Cell 3’ v3.1 Solutions Liberase

(18) The circular RNA Ataxia Telangiectasia Mutated regulates oxidative stress in smooth muscle cells in expanding abdominal aortic aneurysmsMolecular therapy. Nucleic acidsSeptember 12, 2023Fasolo F, Winski G, Li Z, Wu Z, Winter H, Ritzer J, Glukha N, Roy J, Hultgren R, Pauli J, et al.instructions. The cell suspension was strained (70 and 40 μm) and Dead Cell Removal (Miltenyi Biotech, 130-090-101) using MS Columns (Miltenyi Biotech, 130-042-201) was performed. Cells were resuspended in PBS + 0.04%

(19) Single-cell profiling reveals inflammatory polarization of human carotid versus femoral plaque leukocytesJCI InsightSeptember 8, 2023Joshua Slysz, Arjun Sinha, Matthew DeBerge, Shalini Singh, Harris Avgousti, Inhyeok Lee, Kristofor Glinton, Reina Nagasaka, Prarthana Dalal, Shaina Alexandria, et al.live cells and CD45+ cells. Dead cells were removed with a dead cell removal kit (Miltenyi Biotec, 130-090-101) according to manufacturer’s instructions. Suspensions were then enriched for immune cells with CD45+

(20) Functionally distinct cancer-associated fibroblast subpopulations establish a tumor promoting environment in squamous cell carcinomaNature CommunicationsSeptember 5, 2023Sabrina Schütz, Llorenç Solé-Boldo, Carlota Lucena-Porcel, Jochen Hoffmann, Alexander Brobeil, Anke S. Lonsdorf, Manuel Rodríguez-Paredes, Frank Lykocell strainers (Falcon, cat. no. 10788201) and the Dead Cell Removal Kit (Miltenyi Biotec, cat. no. 130-090-101) was then used to remove apoptotic and dead cells. Subsequently, about 20,000 single cells per sample

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(1)

7/13/2020Kit Works Well at Removing Dead Cells Rebecca GuyerOur lab extracts cells from human tissues for downstream analysis. Due to our processing, many dead cells are in our single cell suspension and we need to remove as many as possible before doing any ... Read Review
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6/4/2018Dead Cell Removal Kit Alex RayOur lab studies HIV immunology and the enrichment of live cells for downstream analysis is important for many of our protocols. This kit provides a reliable way to remove dead cells while maintaining ... Read Review
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3/8/2018Dead Cell Removal Kit Lana JagannathanOur lab studies ways to enhance immune responses against HIV infection. As such we perform a lot of in vitro killing assays with different effector cells against HIV infected PBMC. One way to measure ... Read Review
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7/8/2016Dead Cell Separation from Single Cell Preparation Sivaraman NatarajanWe prepare single cells from various tissues for our Genomic studies. We encountered lot of damaged and dead cells in the end from the preparation. This kit works works on MACS ... Read Review

Dead Cell Removal Kit (up to 100 separations)

Dead Cell Removal Kit from Miltenyi Biotec

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Miltenyi Biotec

Miltenyi Biotec

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Dead Cell Removal Kit from Miltenyi Biotec

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