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IPTG
IPTG, Dioxane-Free
IPTG, Dioxane-Free from Promega
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Citations:
(8)
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IPTG, Dioxane-Free
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Description
An inducer of beta-galactosidase activity in many bacteria
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
IPTG, Dioxane-Free
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
V3955
Quantity
1g
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (8)
(1)
Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancer
Cell Reports
May 1, 2025
Alessia Catozzi, Maria Peiris Pagès, Sam Humphrey, Mitchell Revill, Derrick Morgan, Jordan Roebuck, Yitao Chen, Bethan Davies-Williams, Kevin Brennan, A.S. Md. Mukarram Hossain,
et al.
1L of LB with Kanamycin A. OD was checked regularly and induction performed with 1 mM IPTG (
Promega
,
V3955
) when OD reached 0.4 for 3 h. After 3 h, bacterial cultures were pelleted at 3,500 rpm for 15 min and
(2)
Shrimp Virus Regulates ROS Dynamics via the Nrf2 Pathway to Facilitate Viral Replication
Advanced Science
March 16, 2025
Honghui He, Kai Yuan, Junming Pan, Shaoping Weng, Chaozheng Li, Yihong Chen, Jianguo He
A830931) at 37 °C until OD600 reached 0.8. Then, 1 mm isopropyl‐β‐D‐thiogalactopyranoside (IPTG,
Promega
V3955
) was used to induce the expression of wsv220 at 37 °C for 6 h. Proteins were purified using a PierceTM
(3)
USP7 regulates the ERK1/2 signaling pathway through deubiquitinating Raf-1 in lung adenocarcinoma
Cell death & disease
August 10, 2022
Park HB, Hwang S, Baek KH.
tube.
Transfected
cells were induced using 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Cat #
V3955
,
Promega
, Madison, WI, USA) and adjusted to an A600 of 0.4–0.5. Cells were lysed by
sonication
and incubated
(4)
Improving Lipophagy by Restoring Rab7 Cycle: Protective Effects of Quercetin on Ethanol-Induced Liver Steatosis
Nutrients
February 4, 2022
Lin H, Guo X, Liu J, Liu P, Mei G, Li H, Li D, Chen H, Chen L, Zhao Y,
et al
.
ThermoFisher Scientific
(
12566014
). DAPI (C1006) and Hoechst (C1002) were bought from Beyotime. IPTG (
V3955
) and BeadsTM GSH (70601-5) were purchased from
Promega
and
Beaver
. The plasmids of Rab7Wt (W1949Gn),
(5)
Identification of scavenger receptor B1 as the airway microfold cell receptor for
Mycobacterium tuberculosis
eLife
March 5, 2020
Khan HS, Nair VR, Ruhl CR, Alvarez-Arguedas S, Galvan Rendiz JL, Franco LH, Huang L, Shaul PW, Kim J, Xie Y,
et al
.
protein expression
. 1 L of bacterial culture was grown to an OD600 of 0.6, induced with 1 mM IPTG (
Promega
V3955
) at 37˚C for 3 hr, and centrifuged at 3500 rpm for 15 min at 4˚C to yield a bacterial pellet. The bacterial
(6)
Characterizing Protein Kinase Substrate Specificity Using the Proteomic Peptide Library (ProPeL) Approach
Current Protocols in Chemical Biology
June 22, 2018
Joshua M. Lubner, Jeremy L. Balsbaugh, George M. Church, Michael F. Chou, Daniel Schwartz
(see Reagents and Solutions) Isopropyl β-D-1-thiogalactopyranoside (IPTG) (
Promega
cat. no.
V3951
or
V3955
) Tris Base (Sigma Aldrich cat. no. T6066) Sodium Chloride (Fisher Scientific cat. no. S671-500)
(7)
Identification of fibroblast growth factor receptor 3 (FGFR3) as a protein receptor for botulinum neurotoxin serotype A (BoNT/A)
PLoS pathogens
January 1, 2013
Jacky BP, Garay PE, Dupuy J, Nelson JB, Cai B, Molina Y, Wang J, Steward LE, Broide RS, Francis J,
et al
.
E.Coli
, Acella Electrocompetent BL21(DE3) (
42649
;
Edge Biosystems
). Expression was induced by 1 mM IPTG (
V3955
;
Promega
) at either 37°C for 16 hours (FGFR3b/c peptides) or at 16°C for 16 hours (rHC/A).
For purification
(8)
The mu-opioid receptor and the NMDA receptor associate in PAG neurons: implications in pain control
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology
January 1, 2012
Rodríguez-Muñoz M, Sánchez-Blázquez P, Vicente-Sánchez A, Berrocoso E, Garzón J.
C0– C2), and NR1 (segments C0–C1–C2)
strains
were grown to an optical density of 0.5–0.6. IPTG (
Promega
; #
V3955
) and Neuropsychopharmacology Association mu-opioid receptor–NMDA receptor M Rodrı́guez-Muñoz et
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IPTG, Dioxane-Free from Promega
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