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Author Profile
Niroj Shrestha
Postdoctoral Researcher
Medical University of Graz
Articles by Niroj Shrestha
Assay Kit/Reagent User Review
Penicillin-Streptomycin From Millipore Sigma is Ideal for HL-1 Cell Culture
November 14, 2023
Penicillin-Streptomycin is diluted 1:100 in Claycomb medium with 10% FBS, 2 mM L-glutamine, and 0.1 mM noradrenaline and used for ...
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Assay Kit/Reagent User Review
Selected Stable Gene Expression in HEK293 Cells With Disulfate Salt Solution From Merck
October 23, 2023
G418 stock was diluted to 250 mcg/ml in DMEM containing 10% FBS, 4 mM glutamine, 10 mM HEPES and puromycin (5 mcg/ml) and used to ...
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Assay Kit/Reagent User Review
L-Glutamine From Thermo Fisher is Ideal to Culture HL-1 Cells
October 12, 2023
L-glutamine is diluted to 2 mM in Claycomb medium with 10% FBS, 1 % penicillin/streptomycin, and 0.1 mM noradrenaline and used for...
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Antibody User Review
TMEM38A (G-12) Antibody Detects TRIC-A in Cell Lysates
September 06, 2022
Lysate from HEK293 cells with transiently overexpressed TRIC-A-mCherry was resolved in NuPAGE 4%–12% Bis-Tris gel and transferred ...
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Antibody User Review
TMEM38B Antibody Detects Endogenous TMEM38B Protein in HEK293 Cells
September 02, 2022
HEK293 cell lysate was resolved in NuPAGE 4%–12% Bis-Tris gel and transferred to 0.2-µm PVDF membrane using the Trans-Blot Turbo ...
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Equipment User Review
Tacta Mulitchannel Pipette Ideal for qPCR
August 24, 2022
Tacta 12-channel pipette is used for simultaneous pipetting of 30-300 ul of solutions and buffers into 12 wells of deep-well ...
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Assay Kit/Reagent User Review
HEPES Buffer Maintains pH in Cell Culture
August 23, 2022
HEPES diluted to 10 mM in cell culture medium maintains pH in cell culture outside a CO2 incubator during passaging and ...
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Assay Kit/Reagent User Review
MEM NEAA Promotes Cell Culture Growth and Viability
August 23, 2022
MEM NEAA is diluted to 1x in culture medium like DMEM, MEM and used for culture of cell lines like RBL-2H3, EA.hy926.
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Assay Kit/Reagent User Review
Protein Ladder Ideal to Track Protein Bands of 10-260 kDa in Gel and Western Blot
August 09, 2022
5 ul protein ladder is used as a marker in SDS-PAGE gels and nitrocellulose/PVDF membranes to monitor protein bands of 10-260 KDa ...
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Assay Kit/Reagent User Review
Ideal PBS for Washing Cell Lines for Trypsinization and Passaging
August 09, 2022
PBS is routinely used to wash out Ca2+, Mg2+, and serum from various cell lines like HEK293, RBL-2H3, and HL-1 before cell ...
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Assay Kit/Reagent User Review
Prestained Protein Ladder Ideal to Track Protein Bands of 10-250 kDa in Gel and Blot
August 08, 2022
5 ul protein ladder is routinely used as a marker in protein gels and nitrocellulose/PVDF membrane to locate protein bands of 10-...
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Assay Kit/Reagent User Review
Penicillin-Streptomycin is Suitable for Maintaining Sterile Cell Cultures
August 05, 2022
Penicillin-Streptomycin is diluted in cell culture medium to 1x concentration and routinely used to prevent bacterial ...
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Assay Kit/Reagent User Review
TrypLE Express Dissociates Adherent Cell Lines for Passaging
August 05, 2022
1-2 ml TrypLE Express is added to adherent cell lines like RBL-2H3 and HL-1 for a few minutes till the cells appear round. 2 ml of...
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Assay Kit/Reagent User Review
SuperSignal West Pico Works Great as Chemiluminescent Substrate
July 13, 2022
Cells at 80% confluency in 35-mm cell culture dishes are lysed in ice-cold 100 µl RIPA buffer containing 1 µl protease inhibitor ...
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Equipment User Review
Accurate Eppendorf Pipette
July 13, 2022
We routinely use 0.5-10 ul eppendorf pipette in our laboratory for pipetting a small volume of chemicals and buffers during ...
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Product Review
Efficient and Cost Friendly SYBR Green Mastermix for qPCR
Parijat Sarkar
I study the transcription of endogenous genes regulated by transcription factors, and it's crucial to quantify their expression levels accurately. To achieve ...
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