Luciferase Control RNA from Promega

Product Specs
ItemLuciferase Control RNA
CompanyPromega
Price $72.00Supplier Page View Company Product Page
Catalog NumberL4561
Quantity20microg

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(61) Ethanol-Induced Behavioral Sensitization Alters the Synaptic Transcriptome and Exon Utilization in DBA/2J MiceFrontiers in GeneticsSeptember 24, 2018Megan A. O’Brien, Rory M. Weston, Nihar U. Sheth, Steven Bradley, John Bigbee, Ashutosh Pandey, Robert W. Williams, Jennifer T. Wolstenholme, Michael F. Miles1.97 and 2.06. cDNA was generated from 995 ng of total DNase-treated RNA and 5 ng of luciferase mRNA (#L4561, Promega, Madison, WI, United States) using Deoxyribonuclease I (#18068-015, Invitrogen, Carlsbad, CA,

(62) Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff PerfusionJournal of Visualized ExperimentsSeptember 18, 2018Miroslava Stastna, Amandine Thomas, Juliana Germano, Somayeh Pourpirali, Jennifer E. Van Eyk, Roberta A. Gottliebextraction TRIzol Reagent,Life technologies,AM9738,RNA extraction Luciferase Control RNA,Promega,L4561,RNA extraction Chloroform,Fisher Scientific,C606–4,RNA extraction "Glycogen, RNA grade",Thermo Fisher Scientific

(63) A Network of Noncoding Regulatory RNAs Acts in the Mammalian BrainCellJuly 12, 2018Kleaveland B, Shi CY, Stefano J, Bartel DP.least 5 times. 25 pg of uncapped firefly luciferase RNA containing a 30-base poly(A) tail (Promega #L4561) was added to each fraction in TRI Reagent prior to RNA extraction. Lentivirus production and transduction HEK293T

(64) Characterization of lipid metabolism genes and the influence of fatty acid supplementation in the hepatic lipid metabolism of dusky grouper ( Epinephelus marginatus )Comparative Biochemistry and Physiology Part A: Molecular & Integrative PhysiologyMay 1, 2018Bruno C. Araújo, Nicholas M. Wade, Paulo H. de Mello, Jandyr de A. Rodrigues-Filho, Carlos E.O. Garcia, Mariana F. de Campos, Natasha A. Botwright, Diogo T. Hashimoto, Renata G. Moreiracontaining 25 μM of oligo (dT) 20 and 25 μM of random hexamers, along with 400 pg of control luciferase RNA (L4561, Promega, WI, USA). PCR ampliﬁcation was performed for each gene-speciﬁc primer using a pool of DNAse-treated

(65) The RNA-binding protein YBX1 regulates epidermal progenitors at a posttranscriptional levelNature communicationsApril 30, 2018Kwon E, Todorova K, Wang J, Horos R, Lee KK, Neel VA, Negri GL, Sorensen PH, Lee SW, Hentze MW, et al.were captured with oligo(dT)25 magnetic beads (NE Biolabs). Luciferase spike-in control RNA (Promega, L4561) was added during the capture and used for normalization of qRT-PCR results. Eluted and purified proteins

(66) Murine macrophage TLR2-FcγR synergy via FcγR licensing of IL-6 cytokine mRNA ribosome binding and translationPloS oneJanuary 1, 2018Hunt D, Drake LA, Drake JR.250 μl samples of each sucrose gradient fraction were spiked with 1 ng of luciferase RNA (Promega, L4561) to provide both a control for the efficiency of RNA recovery and an exogenous reference for qPCR analysis

(67) Temporal Control of Mammalian Cortical Neurogenesis by m⁶A MethylationCellNovember 2, 2017Yoon KJ, Ringeling FR, Vissers C, Jacob F, Pokrass M, Jimenez-Cyrus D, Su Y, Kim NS, Zhu Y, Zheng L, et al.,Thermo Fisher Scientific,10003D CellTrace CFSE,Thermo Fisher Scientific,C34554 luciferase control RNA,Promega,L4561 TRIzol Reagent,Thermo Fisher Scientific,15596026 4-thiouridine,Carbosynth,T4509 MTSEA biotin- XX,Biotium,90066 Dimethylformamide

(68) Abrin Toxicity and Bioavailability after Temperature and pH TreatmentToxins (Basel)October 13, 2017Christina C. Tam, Thomas D. Henderson, Larry H. Stanker, Xiaohua He, Luisa W. Cheng(L4960), complete amino acid mixture (1 mM, N2111), nuclease-free water (P1193), luciferase mRNA (1 mg/mL, L4561), and the Bright-Glo Luciferase Assay System (10 mL, E2610). The NuPAGE 4–12% Bis-Tris gels and SilverXpress

(69) Accurate, Streamlined Analysis of mRNA Translation by Sucrose Gradient FractionationBio ProtocOctober 5, 2017Soufiane Aboulhouda, Rachael Di Santo, Gabriel Therizols, David Weinberg(Thermo Fisher Scientific, GibcoTM, catalog number: 10010023) 4. Luciferase RNA (Promega, catalog number: L4561) Note: Store aliquotted 100 ng/μl stock at -80 °C. 5. HEPES (Sigma-Aldrich, catalog number: H4034) 6.

(70) Accurate, Streamlined Analysis of mRNA Translation by Sucrose Gradient FractionationBIO-PROTOCOLOctober 5, 2017Soufiane Aboulhouda, Rachael Di Santo, Gabriel Therizols, David Weinberg(Thermo Fisher Scientific, GibcoTM, catalog number: 10010023) Luciferase RNA (Promega, catalog number: L4561) Note: Store aliquotted 100 ng/μl stock at -80 °C. HEPES (Sigma-Aldrich, catalog number: H4034) Magnesium

(71) EINCR1 is an EGF inducible lincRNA overexpressed in lung adenocarcinomasPLOS ONEJuly 21, 2017Karol Nowicki-Osuch, Yaoyong Li, Mairi Challinor, David T. Gerrard, Neil A. Hanley, Andrew D. Sharrockswas collected from the pellet and supernatant fractions (supplemented with luciferase RNA (Promega, L4561)–normalization spike-in) using trizol LS reagent (Life technologies, 10296). RNA levels were quantified

(72) [PCR analysis of the absolute number of copies of human chromosome 18 transcripts in liver and HepG2 cells]Biomeditsinskaya KhimiyaApril 18, 2017Y.Y. Kiseleva, K.G. Ptitsyn, O.V. Tikhonova, S.P. Radko, LK. Kurbatov, I.V. Vakhrushev, V.G. Zgoda, E.A. Ponomarenko, A.V. Lisitsa, A.I. Archakovпрепаратам РНК добавляли известное количество контрольной РНК люциферазы (“Promega”, Франция; кат. № L4561) из расчёта 1000 копий на 1 нг суммарной РНК печени или клеток HepG2. Синтез кДНК проводили с помощью

(73) Effects of canola meal on growth, feed utilisation, plasma biochemistry, histology of digestive organs and hepatic gene expression of barramundi (Asian seabass; Lates calcarifer)AquacultureNovember 1, 2016Diu Thi Ngo, Nicholas M. Wade, Igor Pirozzi, Brett D. Glencrossoligo(dT), 25 µM random 234 hexamers and 400 pg of internal non-endogenous control Luciferase RNA (Promega L4561). 235 Primers used in real-time PCR were specific to each gene (Table 4), and designed by 236 PerlPrimer

(74) Identification of genes involved in reproduction and lipid pathway metabolism in wild and domesticated shrimpsMarine GenomicsAugust 1, 2015Guiomar Rotllant, Nicholas M. Wade, Stuart J. Arnold, Gregory J. Coman, Nigel P. Preston, Brett D. Glencross(Callaghan et al., 2010), 165 which incorporated 400 pg of non-endogenous Luciferase RNA (Promega L4561, 166 Venlo, The Netherlands) as an internal control. 167 Real-time PCR reactions were carried out

(75) Profiling of Cxcl12 Receptors, Cxcr4 and Cxcr7 in Murine Testis Development and a Spermatogenic Depletion Model Indicates a Role for Cxcr7 in Controlling Cxcl12 ActivityPLoS ONEDecember 2, 2014Birgit Westernströer, Nicole Terwort, Jens Ehmcke, Joachim Wistuba, Stefan Schlatt, Nina Neuhausisolation and relative gene expression analysis Per testis sample, 20 ng luciferase mRNA (Promega, L4561, Mannheim, Germany) were added [32], before RNA extraction was performed using Ultraspec (Biotecx, Houston,

(76) Efficient DNA ligation in DNA–RNA hybrid helices by Chlorella virus DNA ligaseNucleic Acids ResearchFebruary 1, 2014Gregory J. S. Lohman, Yinhua Zhang, Alexander M. Zhelkovsky, Eric J. Cantor, Thomas C. Evansµl) were prepared with 2.5 nM each of the splint probes, 1 or 0.01 ng of luciferase mRNA (Promega, L4561) and 50 ng Jurkat cell total RNA (Life Technologies, AM7858) in T4 DNA ligase buffer. Probes were annealed

(77) Loss of tumor suppressor RPL5/RPL11 does not induce cell cycle arrest but impedes proliferation due to reduced ribosome content and translation capacityMolecular and cellular biologyDecember 1, 2013Teng T, Mercer CA, Hexley P, Thomas G, Fumagalli S.fractions were collected by upward displacement, and 50 pg of synthetic luciferase mRNA (catalog number L4561; Promega) and 10 ␮g of glycogen (catalog number AM9510; Life Technology) were added to each fraction

(78) Induced Fungal Resistance to Insect Grazing: Reciprocal Fitness Consequences and Fungal Gene Expression in the Drosophila-Aspergillus Model SystemPLoS ONEAugust 30, 2013Silvia Caballero Ortiz, Monika Trienens, Marko Rohlfscurve analysis and amplicon sequencing. We used an external luciferase control RNA spike (Promega, L4561) for normalizing candidate RNA quantification rather than common fungal house-keeping genes, as suggested

(79) The NOBOX protein becomes undetectable in developmentally competent antral and ovulated oocytesThe International Journal of Developmental BiologyApril 16, 2013Martina Belli, Danilo Cimadomo, Valeria Merico, Carlo A. Redi, Silvia Garagna, Maurizio Zuccottifor at least 12 hr. One picogram (1 ml) of luciferase control RNA (Promega, Milano, Italy, cat. N. L4561) (used as an exogenous marker on which the results were normalised) was added to each tube (Thelie et

(80) Tumor suppression by small molecule inhibitors of translation initiationOncotargetSeptember 1, 2012Limo Chen et al.cat. #AM1200). The effect of #1181 on the translation efficiency of luciferase RNA (Promega, cat. #L4561) was determined by measuring the luminescence with Wallac Envision Reader.

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