Luciferase Control RNA from Promega

Product Specs
ItemLuciferase Control RNA
CompanyPromega
Price $72.00Supplier Page View Company Product Page
Catalog NumberL4561
Quantity20microg

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(41) Chemotherapy-induced transposable elements activate MDA5 to enhance haematopoietic regenerationNature cell biologyJuly 1, 2021Clapes T, Polyzou A, Prater P, Sagar, Morales-Hernández A, Ferrarini MG, Kehrer N, Lefkopoulos S, Bergo V, Hummel B, et al.and 1 h followed by proteinase K (03115836001, Roche). Spike-in luciferase control RNA (15 pg μl) (L4561, Promega) was added and RNA isolation, cDNA and qPCR were performed as described above. FLASH analysis FLASH-seq

(42) Chromatin-Level Differences Elucidate Potential Determinants of Contrasting Levels of Cold Sensitivity in Maize LinesPlant Molecular Biology ReporterJune 1, 2021Maciej Jończyk, Alicja Sobkowiak, Joanna Trzcinska-Danielewicz, Paweł Sowiński(GRMZM2G118637), actin depolymerizing factor (GRMZM2G060702), and synthetic luciferase gene (cat. no. L4561, Promega, USA). Luciferase mRNA (1 ng) was added during the reverse transcription step (O’Shaughnessy

(43) TET-mediated 5-methylcytosine oxidation in tRNA promotes translationJournal of Biological ChemistryJanuary 1, 2021Hui Shen, Robert Jordan Ontiveros, Michael C. Owens, Monica Yun Liu, Uday Ghanty, Rahul M. Kohli, Kathy Fange Liu(Promega L4960). Translation reactions were supplemented with 20 ng/ul of luciferase mRNA (Promega L4561) and with varying amounts of purified small RNAs or tRNAs. Using this in vitro translation system with

(44) YRDC Mediates the Resistance of Lenvatinib in Hepatocarcinoma Cells via Modulating the Translation of KRASFrontiers in pharmacologyJanuary 1, 2021Guo J, Zhu P, Ye Z, Wang M, Yang H, Huang S, Shu Y, Zhang W, Zhou H, Li Q.(2006). In vitro translation was performed using a RRL system (L4540, Promega). 1 μg of luciferase mRNA (L4561, Promega) or 2 μg of KRAS mRNA was added to RRL containing 70 mM KCl, 2 mM DTT, 10 μM minus leucine,

(45) TET-mediated 5-methylcytosine oxidation in tRNA promotes translationJ Biol ChemNovember 23, 2020Hui Shen, Robert Jordan Ontiveros, Michael C. Owens, Monica Yun Liu, Uday Ghanty, Rahul M. Kohli, Kathy Fange Liu(Promega L4960). Translation reactions were supplemented with 20 ng/ul of luciferase mRNA (Promega L4561) and with varying amounts of purified small RNAs or tRNAs. Using this in vitro translation system with

(46) Inhibition of O-GlcNAc Transferase Renders Prostate Cancer Cells Dependent on CDK9Molecular Cancer ResearchOctober 1, 2020Harri M. Itkonen et al.Synthesis Kit (Quantabio). For spike-in RNA normalization, luciferase mRNA was obtained from Promega (L4561). High-throughput screen LNCaP cells were plated into 384-well plate (1000 cells per well). The next

(47) eIF5B drives integrated stress response-dependent translation of PD-L1 in lung cancerNature CancerSeptember 28, 2020Shruthy Suresh et al.isolate RNA and cDNA synthesis and real-time PCR performed. 20ng of Luciferase mRNA control (Promega, L4561) was added to each fraction prior to RNA extraction to control for variability in total RNA in fractions

(48) Protein Stability Buffers the Cost of Translation Attenuation following eIF2α PhosphorylationCell reportsSeptember 15, 2020Schneider K, Nelson GM, Watson JL, Morf J, Dalglish M, Luh LM, Weber A, Bertolotti A.Proteins" ,, Tunicamycin,Sigma-Aldrich,T7765-5MG Cycloheximide,Sigma-Aldrich,Cat#C7698 SYBR green,ThermoFisher Scientific,Cat#44-729-08 Luciferase RNA,Promega,L4561 SuperScript II,ThermoFisher Scientific,18064014 SuperScript IV,ThermoFisher Scientific,18091050 ,, Critical

(49) Dietary starch promotes hepatic lipogenesis in barramundi (Lates calcarifer)British Journal of NutritionAugust 28, 2020N. M. Wade et al.III (Invitrogen) with 25 μM oligo(dT)20, 25 μM random hexamers and 400 pg of luciferase RNA (Promega L4561) as an exogenous control gene. Real-time PCR amplification using primers specific to each gene of interest

(50) Persistent Activation of mRNA Translation by Transient Hsp90 InhibitionCell ReportsAugust 11, 2020Peter Tsvetkov, Timothy J. Eisen, Sven U. Heinrich, Zarina Brune, Erinc Hallacli, Greg A. Newby, Can Kayatekin, David Pincus, Susan Lindquist1006–100 Galcto-star,ThermoFisher,Cat# T1012 X-gal,Sigma-Aldrich,Cat# 10703729001 Luciferase Control RNA,Promega,Cat# L4561 TRIzol Reagent,ThermoFisher,Cat# 15596026 RNasin® Ribonuclease Inhibitor,Promega,Cat# N2111 "cOmplete,

(51) Inhibition of O-GlcNAc transferase renders prostate cancer cells dependent on CDK9Mol Cancer ResJuly 1, 2020Harri M. Itkonen, Ninu Poulose, Rebecca E. Steele, Sara E. S. Martin, Zebulon G. Levine, Damien Y. Duveau, Ryan Carelli, Reema Singh, Alfonso Urbanucci, Massimo Loda, et al.Synthesis Kit (Quantabio). For spike-in RNA normalization, luciferase mRNA was obtained from Promega (L4561). High-throughput screen LNCaP cells were plated into 384-well plate (1000 cells per well). The next

(52) Post-Transcriptional Regulation of Alpha One Antitrypsin by a Proteasome InhibitorInternational journal of molecular sciencesJune 17, 2020Rao L, Xu Y, Reineke LC, Bhattacharya A, Tyryshkin A, Shin JN, Eissa NT.the absorbance at 254 nm by UA-6 Detector. After collecting fractions, spike-in control luciferase mRNA L4561 (Promega, Madison, WI, USA) was add into each sample to a final 10 ng/mL. RNA from each fraction of

(53) Adequate supply of dietary taurine stimulates expression of molecular markers of growth and protein turnover in juvenile barramundi (Lates calcarifer)Fish Physiology and BiochemistryJune 1, 2020David A. Poppi, Stephen S. Moore, Nicholas M. Wade, Brett D. Glencross(Resuehr and Spiess, 2003), incorporating 400 pg of an exogenous Luciferase (Luc) RNA template (Promega L4561) as an internal control (De Santis et al., 2011). Real-time PCR primers for those genes not previously

(54) PIWI–piRNA pathway-mediated transposable element repression in Hydra somatic stem cellsRNAMay 1, 2020Bryan B. Teefy, Stefan Siebert, Jack F. Cazet, Haifan Lin, Celina E. Julianovolume was removed and saved as the “input” sample. After IP, 1 ng of Luciferase Control RNA (Promega L4561) was added to the washed beads as a positive control for subsequent RNA extraction and cDNA synthesis.

(55) Suppression of MEHMO Syndrome Mutation in eIF2 by Small Molecule ISRIBMolecular cellFebruary 20, 2020Young-Baird SK, Lourenço MB, Elder MK, Klann E, Liebau S, Dever TE.10296028 Dynabeads M270 Epoxy,Thermo Fisher Scientific,Cat#: 14301 Luciferase Control RNA,Promega,Cat#: L4561 cOmplete EDTA-free protease inhibitor cocktail,Sigma-Aldrich,Cat#: 11873580001 Polybrene Infection/Transfection Reagent

(56) High-throughput screening and classification of chemicals and their effects on neuronal gene expression using RASL-seqScientific ReportsDecember 1, 2019Jeremy M. Simon, Smita R. Paranjape, Justin M. Wolter, Gabriela Salazar, Mark J. Zylkato a final concentration of 20 nM. A luciferase spike-in mRNA (Promega Corporation, catalog number L4561) was added to the probe pool at a final concentration of 1 nM. (Supplementary Data S1). Barcoding primers

(57) CDK9 Inhibition Induces a Metabolic Switch that Renders Prostate Cancer Cells Dependent on Fatty Acid OxidationNeoplasiaJuly 1, 2019Harri M. Itkonen, Ninu Poulose, Suzanne Walker, Ian G. MillsillustraMiniSpin-kit (GE Healthcare) according to manufacturer's instructions, except luciferase RNA (Promega, cat # L4561) was added into cell lysis buffer. cDNA was synthesized using the qScript cDNA Synthesis Kit (Quantabio).

(58) Dynamic expression of tRNA-derived small RNAs define cellular statesEMBO reportsJuly 1, 2019Krishna S, Yim DG, Lakshmanan V, Tirumalai V, Koh JL, Park JE, Cheong JK, Low JL, Lim MJ, Sze SK, et al.P‐value < 0.05 (Dataset EV4). In vitro translation assay 300 ng of uncapped Firefly luciferase mRNA (L4561, Promega) was used in a 25 μl rabbit reticulocyte lysate system (L4960, Promega) and incubated at 30°C

(59) Postprandial plasma free amino acid profile and hepatic gene expression in juvenile barramundi (Lates calcarifer) is more responsive to feed consumption than to dietary methionine inclusionAquacultureFebruary 1, 2019David A. Poppi, Stephen S. Moore, Nicholas M. Wade, Brett D. Glencrossthe Superscript III first strand synthesis system (Invitrogen) with 25 μM oligo(dT)20 SC (Promega L4561) as an internal control (De Santis et al., 2011).. RI and 25 μM random hexamers, incorporating 400

(60) Ethanol-Induced Behavioral Sensitization Alters the Synaptic Transcriptome and Exon Utilization in DBA/2J MiceFrontiers in GeneticsSeptember 24, 2018Megan A. O’Brien, Rory M. Weston, Nihar U. Sheth, Steven Bradley, John Bigbee, Ashutosh Pandey, Robert W. Williams, Jennifer T. Wolstenholme, Michael F. Miles1.97 and 2.06. cDNA was generated from 995 ng of total DNase-treated RNA and 5 ng of luciferase mRNA (#L4561, Promega, Madison, WI, United States) using Deoxyribonuclease I (#18068-015, Invitrogen, Carlsbad, CA,

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