Luciferase Control RNA from Promega

Product Specs
ItemLuciferase Control RNA
CompanyPromega
Price $72.00Supplier Page View Company Product Page
Catalog NumberL4561
Quantity20microg

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(21) Liver cancer initiation requires translational activation by an oncofetal regulon involving LIN28 proteinsThe Journal of clinical investigationJune 13, 2024Hsieh MH, Wei Y, Li L, Nguyen LH, Lin YH, Yong JM, Sun X, Wang X, Luo X, Knutson SK, et al.RNA; then cDNA synthesis and RT-qPCR were performed, and 20 ng of Luciferase mRNA control (Promega, L4561) was added to each fraction prior to RNA extraction to control for variability in total RNA in fractions

(22) The Development of a One-Step RT-qPCR for the Detection and Quantification of Viable Forms of Trypanosoma cruzi in Açai Samples from Areas at Risk of Chagas Disease through Oral TransmissionInternational Journal of Molecular SciencesMay 18, 2024Amanda Faier-Pereira, Paula Finamore-Araujo, Carlos Ramon do Nascimento Brito, Eldrinei Gomes Peres, Klenicy Kazumy de Lima Yamaguchi, Daniele Pereira de Castro, Otacilio C. Moreira[19]" RNA-IAC (VIC/NFQ-MGB),Forward,5′-TAC AAC ACC CCA ACA TCT TCG A-3′,Not Available,"Promega (Cat. Number: L4561), Johnson et al., 2005 [35]" RNA-IAC (VIC/NFQ-MGB),Not Available,"Promega (Cat. Number: L4561), Johnson

(23) Mechanisms of antiviral action and toxicities of ipecac alkaloids: Emetine and dehydroemetine exhibit anti-coronaviral activities at non-cardiotoxic concentrationsVirus ResearchMarch 1, 2024Viktoriya S. Sidorenko, Ira Cohen, Kunchok Dorjee, Conceição A. Minetti, David P. Remeta, Junyuan Gao, Irina Potapova, Hong Zhan Wang, Janet Hearing, Wan-Yi Yen, et al.reactions (20 μl) contained 80 % TNT Quick master mix, 20 µM methionine, 40 ng/µL luciferase RNA (Promega; L4561), and test chemicals. Samples were incubated at 30 °C for 90 min and diluted 10-fold with PBS. An aliquot

(24) Targeting eIF4A triggers an interferon response to synergize with chemotherapy and suppress triple-negative breast cancerThe Journal of clinical investigationDecember 15, 2023Zhao N, Kabotyanski EB, Saltzman AB, Malovannaya A, Yuan X, Reineke LC, Lieu N, Gao Y, Pedroza DA, Calderon SJ, et al.hundred fifty microliters of each fraction was mixed with 500 pg of luciferase RNA spike-in (Promega, L4561) and lysed in 750 μL TRIzol LS (Thermo Fisher Scientific, 10296028). RNA was precipitated in the presence

(25) METTL3 alters capping enzyme expression and its activity on ribosomal proteinsbioRxivNovember 26, 2023Daniel del Valle-Morales, Giulia Romano, Patricia Le, Michela Saviana, Rachel Brown, Lavender Micalo, Howard Li, Alessandro La La Ferlita, Giovanni Nigita, Patrick Nana-Sinkam, et al.Culjkovic-Kraljacic et. al. [15]. 4μg of total RNA spiked with 1ng/μL of Luciferase Control RNA (Promega #L4561) as a negative uncapped control. 10% of RNA and spike-in was taken as input. Bound RNA was purified

(26) Developmental dynamics of mitochondrial mRNA abundance and editing reveal roles for temperature and the differentiation-repressive kinase RDK1 in cytochrome oxidase subunit II mRNA editingmBioOctober 31, 2023Joseph T. Smith, Brianna Tylec, Arunasalam Naguleswaran, Isabel Roditi, Laurie K. ReadTRIzol, 99 µL of whole cell lysis buffer, and 1 µL of luciferase control RNA (1 mg/mL) (Promega; Cat. No: L4561) were mixed, divided into 40 µL aliquots (to avoid excessive freeze-thawing), and stored at −80°C. After

(27) Decapping factor Dcp2 controls mRNA abundance and translation to adjust metabolism and filamentation to nutrient availabilityElifeJune 2, 2023Anil Kumar Vijjamarri, Xiao Niu, Matthew D Vandermeulen, Chisom Onu, Fan Zhang, Hongfang Qiu, Neha Gupta, Swati Gaikwad, Miriam L Greenberg, Paul J Cullen, et al.total RNA was treated with DNase I (Roche, 4716728001) and 40 pg of Luciferase Control RNA (Promega L4561) was added to 1 µg of DNase I-treated total RNA and subjected to cDNA synthesis using a Superscript

(28) Decapping factor Dcp2 controls mRNA abundance and translation to adjust metabolism and filamentation to nutrient availabilityeLifeJune 2, 2023Anil Kumar Vijjamarri, Xiao Niu, Matthew D Vandermeulen, Chisom Onu, Fan Zhang, Hongfang Qiu, Neha Gupta, Swati Gaikwad, Miriam L Greenberg, Paul J Cullen, et al.total RNA was treated with DNase I (Roche, 4716728001) and 40 pg of Luciferase Control RNA (Promega L4561) was added to 1 µg of DNase I-treated total RNA and subjected to cDNA synthesis using a Superscript

(29) Short-term stability of wastewater samples for storage and shipment in the context of the EU Sewage Sentinel System for SARS-CoV-2Journal of Environmental Chemical EngineeringApril 1, 2023S. Tavazzi, C. Cacciatori, S. Comero, D. Fatta-Kassinos, P. Karaolia, I.C. Iakovides, P. Loutsiou, I. Gutierrez-Aguirre, Z. Lengar, I. Bajde, et al.Slovenia","Maxwell® RSC Enviro Total Nucleic Acid Kit (Promega, AS1831)and Luciferase control RNA (Promega L4561)","Maxwell® RSC Enviro Total Nucleic Acid Kit (Promega, AS1831)",One-step RT-qPCR on an ABI 7900 fast

(30) An RNA stem-loop functions in conjunction with an upstream open reading frame to direct preferential translation in the integrated stress responseThe Journal of biological chemistryFebruary 1, 2023Amin PH, Carlson KR, Wek RC.fractions in response to Tg treatment, 3 μl of 1 μg/ml firefly luciferase control RNA (Promega Cat No L4561) was spiked into each pooled sample to ensure for PCR normalization between fractions. 750 μl of Trizol

(31) A noncanonical RNA-binding domain of the fragile X protein, FMRP, elicits translational repression independent of mRNA G-quadruplexesJournal of Biological ChemistryDecember 1, 2022MaKenzie R. Scarpitti, Julia E. Warrick, Evelyn L. Yoder, Michael G. Kearseextracted from 400 μl of each fraction (spiked with 0.2 ng exogenous control FFLuc mRNA; Promega # L4561) by adding 600 μl TRIzol (Thermo Fisher # 15596018) and following the manufacturer’s protocol. Glycogen

(32) CYFIP1 Dosages Exhibit Divergent Behavioral Impact via Diametric Regulation of NMDA Receptor Complex Translation in Mouse Models of Psychiatric DisordersBiological psychiatryNovember 15, 2022Kim NS, Ringeling FR, Zhou Y, Nguyen HN, Temme SJ, Lin YT, Eacker S, Dawson VL, Dawson TM, Xiao B, et al.Drug,QIAzol Lysis Reagent,Qiagen,79306, Chemical Compound or Drug,luciferase spike-in control RNA,Promega,L4561, Chemical Compound or Drug,TRIzol reagent,Thermo Fisher Scientific,15596026, Chemical Compound or

(33) Evaluation of Methods and Processes for Robust Monitoring of SARS-CoV-2 in WastewaterFood and Environmental VirologyAugust 23, 2022Olivera Maksimovic Carvalho Ferreira et al.of nuclease-free water added instead of the sample. Two ng of Luciferase Control RNA (Promega, USA, L4561) was spiked into each sample and NCE prior to extraction, to confirm the success of the extraction and

(34) Multivalent interactions with RNA drive recruitment and dynamics in biomolecular condensates in Xenopus oocytesiScienceAugust 19, 2022Sarah E. Cabral, Jessica P. Otis, Kimberly L. Mowry(2021),qPCR primer XBM qPCR reverse,Neil et al. (2021),qPCR primer Luciferase control RNA,Promega,Cat#L4561 ,, Recombinant DNA,Recombinant DNA,Recombinant DNA ,, pET:THMT,Peti and Page (2007),N/A pET:THMT:PTBP3,This

(35) Activation and execution of the hepatic integrated stress response by dietary essential amino acid deprivation is amino acid specificThe FASEB JournalJuly 1, 2022William O. Jonsson, Emily T. Mirek, Ronald C. Wek, Tracy G. Anthonycollected and combined into a “heavy” pool (Figure S4B). Five nanograms of luciferase control RNA (L4561, Promega, Madison, WI, USA) was spiked‐in into each combined sample after which 3 ml of TRI Reagent

(36) Activation and execution of the hepatic integrated stress response by dietary essential amino acid deprivation is amino acid specificFASEB JJune 12, 2022William O. Jonsson, Emily T. Mirek, Ronald C. Wek, Tracy G. Anthonycollected and combined into a “heavy” pool (Figure S4B). Five nanograms of luciferase control RNA (L4561, Promega, Madison, WI, USA) was spiked‐in into each combined sample after which 3 ml of TRI Reagent

(37) Characterization of Aurintricarboxylic Acid (ATA) Interactions with Plasma Transporter Protein and SARS-CoV-2 Viral Targets: Correlation of Functional Activity and Binding EnergeticsLife (Basel)June 10, 2022Conceição A. Minetti, David P. Remeta, Keiji Hashimoto, Radha Bonala, Rajesh Chennamshetti, Xingyu Yin, Miguel Garcia-Diaz, Arthur P. Grollman, Francis Johnson, Viktoriya S. Sidorenko(20 μL) contained 80% TNT Quick master mix, 20 µM methionine, and 40 ng/µL luciferase RNA (Promega; L4561) at the indicated concentration of test chemicals. Samples were incubated at 30 °C for 90 min and diluted

(38) Free fatty acid treatment of mouse preimplantation embryos demonstrates contrasting effects of palmitic acid and oleic acid on autophagyAmerican journal of physiology. Cell physiologyMay 1, 2022Leung ZCL, Abu Rafea B, Watson AJ, Betts DH.RNA extraction of mouse embryos, according to manufacturer’s instructions. Exogenous luciferase mRNA (L4561; Promega Corporation, Madison, WI) was added into RNA samples based on the number of embryos in each

(39) L-bodies are RNA-protein condensates driving RNA localization in Xenopus oocytesMolecular biology of the cellDecember 1, 2021Neil CR, Jeschonek SP, Cabral SE, O'Connell LC, Powrie EA, Otis JP, Wood TR, Mowry KL.Tubulin peak pool (non-LB) fractions after SE chromatography. Briefly, 2.5 pg of luciferase RNA (Promega #L4561) were applied to samples just prior to RNA extraction which was performed using TriZol (Invitrogen)

(40) Non-Invasive Characterization of the Pancreas During Bariatric Surgery via Circulating Pancreatic Specific Cell-free Messenger RNAFrontiers in GeneticsOctober 11, 2021Kong Kiat Whye, E ShyongTai, Asim Shabbir, Chin Meng Khoo, Winston KohcfRNA per sample. Spiked-In Quality Controls 105 copies of Luciferase Control RNA (Promega, Cat no. L4561) was spiked in with 10 μL of the extracted cfRNA at the reverse transcription step. It was used as a

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