E220 Focused-ultrasonicator from Covaris LLC

Product Specs
ItemE220 Focused-ultrasonicator
CompanyCovaris LLC
Price Get Pricing Get Quote for this Product
Catalog Number500239
Dimensions(WxDxH) 24 x 30 x 19 in. (61cm x 76cm x 48 cm)
ApplicationsDNA/RNA/Chromatin Shearing:DNA/RNA/Total NA/Protein Extraction from FFPE:cfDNA Extraction from Plasma:DNA Extraction from Dried Blood Spots:Cell lysis: mammalian, bacterial, yeast:Tissue lysis and extraction
Regulatory StatusCE, ETL Mark (for Product Safety), WEEE
MethodAcoustic Energy (Ultrasonic)
Sample VesslesAll AFA Tubes
QuantityEA
Electrical Requirements100-240 VAC 500 VA, 50-60 Hz
TemperatureAmbient temperature: 19° C to 25° C (66° F to 77° F) Relative humidity: 30% to 70%
Volume15 to 130 µl
ComponentsmicroTUBE 8-strip (rack required):microTUBE 96 plate

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Covaris LLC

14 Gill St, Unit H

Woburn, Massachusetts 01801-1721

United States

Phone: +1 781-932-3959
Fax: +1 781-932-8705

Email: info@covaris.com

Company Profile

Website: www.covaris.com

(1) Increasing plant protein sources in the diet modulates gut microbiota and tryptophan metabolism in men at cardiometabolic riskGut MicrobesDecember 31, 2026Gaïa Lépine, Anne-Marie Davila, Gwendal Cueff, Gisèle Pickering, Farid Ichou, Caroline Perreau, Catherine Lefranc-Millot, Marine Gilles, Florence Thirion, François Mariotti, et al.Santa Clara, US) using the Genomic 50 kb Kit. 500 ng of DNA was fragmented by sonication using the E220 focused ultrasonicator (Covaris, Woburn, US) and then underwent double purification using the DNA Clean Beads Kit (MGI Technology,

(2) A complete genome for the common marmosetCellAugust 6, 2026Prajna Hebbar, Tamara Potapova, Hailey Loucks, Karina Ray, Murillo F. Rodrigues, Fedor Ryabov, Joanna Malukiewicz, DongAhn Yoo, Leonardo Gomes Gomes de Lima, Annat Haber, et al.HyperPrep kit, according to the manufacturer’s protocols. The protocol entails shearing the DNA using the E220 Focused-ultrasonicator (Covaris), size selection targeting 400 bp, ligating Illumina specific barcoded adapters, and PCR amplification.

(3) Tissue-resident immune cells drive genetic risk in autoimmune and lung diseasesNat ImmunolAugust 3, 2026Benjamin J. Schmiedel, Cristian Gonzalez-Colin, Vicente Fajardo-Rosas, Job Rocha, Hayley Simon, Johannes Ottensmeier, Ignacio E. Ramírez-Bernabé, April Cano, Angel De la Cruz Castillo, Elizabeth Márquez-Gómez, et al.DNA was isolated using the DNeasy Blood & Tissue Kit (QIAGEN), quantified and sheared using a Covaris E220 Focused-Ultrasonicator. The sonicated DNA was utilized for library preparation using the NEBNext Ultra II DNA Library Prep Kit for Illumina

(4) Microbiota-derived 10-hydroxystearic acid activates PPARα to restore gut epithelial barrier integrity and enhance anti-retroviral therapyNat MicrobiolJuly 30, 2026Dylan Kramer, Clarissa Santos Rocha, Christopher A. Gaulke, Marie Nearing, Sumathi Sankaran-Walters, Ikaika Loque, Anugraha Kidigannappa, Eric Pham, Shuang Hu, Patrawin Wanakumjorn, et al.homogenizer. Approximately 2 × 107 cells were used per treatment. Sonication was conducted using the Covaris E220 focused ultrasonicator (Covaris) for 480 s at the UC Davis DNA Technologies Core. To precipitate chromatin associated with

(5) PLA2G2F suppresses ferroptosis through phospholipid remodelingNature Structural & Molecular BiologyJuly 22, 2026Yingying Hu, Joleen Csuka, Presha Rajbhandari, Penghsuan Huang, Nicholas Carbone, Deguang Liang, Wei Gu, Alban Ordureau, Brent R. Stockwell, Xuejun Jiangglycine. Nuclei were isolated, resuspended in SDS immunoprecipitation buffer and sheared using a Covaris E220 Focused-ultrasonicator for 15 min to yield DNA fragments spanning 200–600 bp. Sheared chromatin was cleared by centrifugation

(6) Comprehensive molecular characterization of cutaneous squamous cell carcinoma reveals determinants of metastatic progressionmedRxivJuly 20, 2026Barbara Rentroia-Pacheco, Harsh Sharma, Lara Pozza, Joleen J.H. Traets, Bishal Tandukar, Olivia F.M. Steijlen, Romy Ruiter, Noel Cruz-Pacheco, Daphne Huigh, Arne Van Hoeck, et al.of 4 and 8 μm. The DNA was sheared to an average fragment size of 300 base pairs using the Covaris E220 focused ultrasonicator. Libraries were constructed with the KAPA HyperPrep Kit (Roche, cat. no 07962363001) and enriched for

(7) Using Nuclear Genomic Data to Address Intractable Relationships and Gene Tree Discordance in an Ancient Group of Gymnosperms (Ephedra, Gnetales)Ecol EvolJune 30, 2026Ruben Blokzijl, Catarina Rydin(1%) electrophoresis, DNA samples were sheared to a set target insert size of 350 bp with a Covaris E220 Focused‐ultrasonicator (Covaris, Woburn, MA, United States) at Science for Life Laboratory (Solna, Sweden). DNA libraries were

(8) A pilot translational study of neoadjuvant fulvestrant plus abemaciclib in women with advanced low-grade serous carcinomaNature CommunicationsJune 29, 2026Lauren P. Cobb, Yibo Dai, Max Molina Ayala, Nejla Ozirmak Lermi, Sharia D. Hernandez, Joseph Davis, Sanghoon Lee, Barrett Craig Lawson, Richard A. Hajek, Joseph Celestino, et al.of gDNA per sample, as determined by PicoGreen quantification, was mechanically fragmented using the E220 Focused-ultrasonicator (Covaris) under the following parameters: Peak Incident Power 200, Duty Cycle 25%, Cycles per Burst

(9) Identifying genetic causes and establishing a diagnostic approach for WES-negative pediatric population with neurodevelopmental disorderEuropean Journal of Human GeneticsJune 23, 2026Yeseul Kim, Joowon Jang, Kyeong Seon Ryu, Jong-Hee Chae, Jung Min Ko, Man Jin Kim, Seungbok Lee, Jangsup Moon, Jin Sook Lee, Hoyeon Lee, et al.blood using the Chemagic 360 instrument (Perkin Elmer, Baesweiler, Germany), fragmented with a Covaris E220 focused ultrasonicator (Covaris, Woburn, MA, USA), and assessed using the TapeStation 4200 (Agilent Technologies, Santa Clara,

(10) Exposure to multiple metallic elements and risk of thyroid tumors: insights from elemental profiling, diet, and molecular characteristics plasma levels of metallic elementsFrontiers in OncologyJune 18, 2026Chao Zhai, Jianyun Sheng, Liang Chen, Long Jia, Chaoyang Wang, Tuo Han, Peina He, Shushen Ji, Mengxin Zhao, Dong Xiaototal of 30 ng of each gDNA sample based on Qubit quantification were mechanically fragmented on an E220 focused ultrasonicator Covaris (Covaris, Woburn, MA). Sheared gDNA (200 ng) was used to perform end repair, and A-tailing and

(11) Molecular mechanisms of the MLL4 complex in H3K4 methylation and p53-dependent transcription activationMolecular CellJune 18, 2026Jianfeng Sun, Zhiying Zhang, You Yu, Nicole Cruz, Sicong Zhang, Yufei Xiang, Keiichi Ito, Cole Lazarus, Zhanyun Tang, Buren Li, et al.added to a final concentration of 1%. Chromatin extracts were sonicated for 15 minutes using a Covaris E220-focused ultrasonicator. Lysates were centrifuged at maximum speed for 10 minutes at 4°C, and 5% of supernatant was saved as

(12) Biomarker study of pembrolizumab in patients with advanced rare cancersCell Rep MedJune 16, 2026Aung Naing, Xiqi Li, Qi Wang, Xiangjun (Jun) Tian, Sharjeel Sabir, Priya R. Bhosale, Mohamed H. Derbala, Bettzy Stephen, Mingxuan Xu, Joud Hajjar, et al.gDNA/cDNA sample based on the PicoGreen quantification was sheared (mechanically fragmented) using the E220 Focused-ultrasonicator Covaris (Covaris). The sonication was performed under the following conditions: Peak Incident Power

(13) Dose-dependent hormone actions at estrogen receptor enhancers specify distinct molecular and biological outcomesSci AdvJune 5, 2026Hyung Bum Kim, Tulip Nandu, Yoon Jung Kim, Cristel V. Camacho, W. Lee KrausLibrary Prep Module (Arima, A101030). Briefly, biotinylated and ligated DNA was sheared using a Covaris E220 Focused-ultrasonicator at 4°C, 105 peak incident power, 5% duty factor, and 200 cycles per burst for 70 s. Sizes were confirmed

(14) NeoCircle: pre- and post-operative circulating tumor DNA dynamics predicts survival in neoadjuvant-treated early breast cancerEMBO Molecular MedicineMay 26, 2026Anthony M George, Yilun Chen, Sergii Gladchuk, Miguel Alcaide, Hina Dalal, Pei Meng, Christian Brueffer, Hani Saghir, Siker Kimbung, Kristina Aaltonen, et al.instrument,Thermo Fisher Scientific,Q33327 E220 or ML230 Focused-ultrasonicator instrument,Covaris,"500239, 500656" QIAseq UDI Y-Adapter sequences,Qiagen,"180312, 180314, 180316, 180318" Pippin HT instrument,Sage

(15) Short-chain fructo-oligosaccharides modulate gut microbiota composition and metabolism: dose-response assessment in an ex vivo gut modelGut Microbes RepMay 25, 2026Christelle Bressuire, Florence Thirion, Laura Chiaravano, Serigne Inssa Ngom, Romane Marion, Marine Gilles, Benoît Quinquis, Elliot Mathieu, Magali Berland, Hervé M. Blottière, et al.Clara, US) using the Genomic 50 kb kit. About 500 ng of DNA was fragmented by sonication using the E220 Focused-ultrasonicator (Covaris, Woburn, USA) and then underwent double purification using the DNA Clean Beads Kit (MGI Technology,

(16) The impact of APC mutation on the efficacy of anti-EGFR therapy in metastatic colorectal cancer with RAS wild type: a retrospective analysisTher Adv Med OncolMay 23, 2026Junkyu Kim, Changgon Kim, Ji Eun Shin, Sung Hee Lim, Seung Tae KimQubit 2.0 Fluorometer (Thermo Fisher Scientific) was used to quantify the 40 ng of DNA. The Covaris E220 Focused-ultrasonicator (Woburn, MA, USA) and the eight microTUBE–50 Strip AFA Fiber V2 were used for shearing. The treatment

(17) Integrated genomics and transcriptomics reveal key events in pancreatic neuroendocrine neoplasmBMC BiologyMay 19, 2026Shiwei Guo, Xiaohan Shi, Xiaoyi Yin, Suizhi Gao, Bo Li, Huan Wang, Yikai Li, Beilei Wang, Xiongfei Xu, Kailian Zheng, et al.Technologies, Santa Clara, USA). DNA fragmentation to approximately 350 bp was achieved with a Covaris E220 Focused-ultrasonicator (Covaris, Brighton, UK). The fragmented DNA underwent end-repair, A-tailing, and adapter ligation with

(18) The ARTEMIS trial identifies immune activation as a key predictor of neoadjuvant chemotherapy response in triple-negative breast cancerBreast Cancer ResearchMay 6, 2026Sahil Seth, Clinton Yam, Lei Huo, Bora Lim, Amanda L. Rinkenbaugh, Jason B. White, Elizabeth E. Ravenberg, Xingzhi Song, Jennifer K. Litton, Debu Tripathy, et al.each cDNA sample based on the PicoGreen quantification was sheared (mechanically fragmented) using the E220 Focused-ultrasonicator Covaris (Covaris). The sonication was performed under the following conditions: Peak Incident Power

(19) ASB7 promotes osteosarcoma lung metastasis through ubiquitin-mediated degradation of ATF2Cell DiscovMay 5, 2026Yezi Zou, Jianliang Zhong, Lanqing Huo, Jiali Chen, Xinhao Yu, Jingxuan Wang, Zhenxuan Chen, Lifeng Yin, Cuiling Zeng, Xia Zhang, et al.for 30 min at room temperature. Chromatin was then sheared into 200–400 bp fragments using a Covaris E220 focused ultrasonicator. The sheared chromatin supernatant was obtained by centrifugation at 12,000 × g for 10 min at 4 °C,

(20) The conserved N-terminal SANT1-binding domain (SBD) of EZH2 regulates PRC2 activityGenes & developmentMay 4, 2026Patriotis AL, Barrows DW, Soto-Feliciano Y, Khan LF, Leboeuf M, Marchione DM, Marunde MR, Keogh MC, Carroll TS, Garcia BA, et al.passing twice through a 27 gauge needle. Chromatin extracts were sonicated for 14 min using a Covaris E220 focused ultrasonicator at peak power 140, duty factor 5, and cycles/burst 200. For ChIP of histone posttranslational modifications,

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