E220 Focused-ultrasonicator from Covaris LLC

Product Specs
ItemE220 Focused-ultrasonicator
CompanyCovaris LLC
Price Get Pricing Get Quote for this Product
Catalog Number500239
Dimensions(WxDxH) 24 x 30 x 19 in. (61cm x 76cm x 48 cm)
ApplicationsDNA/RNA/Chromatin Shearing:DNA/RNA/Total NA/Protein Extraction from FFPE:cfDNA Extraction from Plasma:DNA Extraction from Dried Blood Spots:Cell lysis: mammalian, bacterial, yeast:Tissue lysis and extraction
MethodAcoustic Energy (Ultrasonic)
QuantityEA
Regulatory StatusCE, ETL Mark (for Product Safety), WEEE
Sample VesslesAll AFA Tubes
Electrical Requirements100-240 VAC 500 VA, 50-60 Hz
TemperatureAmbient temperature: 19° C to 25° C (66° F to 77° F) Relative humidity: 30% to 70%
Volume15 to 130 µl
ComponentsmicroTUBE 8-strip (rack required):microTUBE 96 plate

Add to Compare List

Covaris LLC

14 Gill St, Unit H

Woburn, Massachusetts 01801-1721

United States

Phone: +1 781-932-3959
Fax: +1 781-932-8705

Email: info@covaris.com

Company Profile

Website: www.covaris.com

(1) Increasing plant protein sources in the diet modulates gut microbiota and tryptophan metabolism in men at cardiometabolic riskGut MicrobesDecember 31, 2026Gaïa Lépine, Anne-Marie Davila, Gwendal Cueff, Gisèle Pickering, Farid Ichou, Caroline Perreau, Catherine Lefranc-Millot, Marine Gilles, Florence Thirion, François Mariotti, et al.Santa Clara, US) using the Genomic 50 kb Kit. 500 ng of DNA was fragmented by sonication using the E220 focused ultrasonicator (Covaris, Woburn, US) and then underwent double purification using the DNA Clean Beads Kit (MGI Technology,

(2) Short-chain fructo-oligosaccharides modulate gut microbiota composition and metabolism: dose-response assessment in an ex vivo gut modelGut Microbes RepMay 25, 2026Christelle Bressuire, Florence Thirion, Laura Chiaravano, Serigne Inssa Ngom, Romane Marion, Marine Gilles, Benoît Quinquis, Elliot Mathieu, Magali Berland, Hervé M. Blottière, et al.Clara, US) using the Genomic 50 kb kit. About 500 ng of DNA was fragmented by sonication using the E220 Focused-ultrasonicator (Covaris, Woburn, USA) and then underwent double purification using the DNA Clean Beads Kit (MGI Technology,

(3) The impact of APC mutation on the efficacy of anti-EGFR therapy in metastatic colorectal cancer with RAS wild type: a retrospective analysisTher Adv Med OncolMay 23, 2026Junkyu Kim, Changgon Kim, Ji Eun Shin, Sung Hee Lim, Seung Tae KimQubit 2.0 Fluorometer (Thermo Fisher Scientific) was used to quantify the 40 ng of DNA. The Covaris E220 Focused-ultrasonicator (Woburn, MA, USA) and the eight microTUBE–50 Strip AFA Fiber V2 were used for shearing. The treatment

(4) ASB7 promotes osteosarcoma lung metastasis through ubiquitin-mediated degradation of ATF2Cell DiscovMay 5, 2026Yezi Zou, Jianliang Zhong, Lanqing Huo, Jiali Chen, Xinhao Yu, Jingxuan Wang, Zhenxuan Chen, Lifeng Yin, Cuiling Zeng, Xia Zhang, et al.for 30 min at room temperature. Chromatin was then sheared into 200–400 bp fragments using a Covaris E220 focused ultrasonicator. The sheared chromatin supernatant was obtained by centrifugation at 12,000 × g for 10 min at 4 °C,

(5) Redox-triggered USP18 confers cisplatin resistance in ovarian cancer by selectively activating a non-canonical FSP1-dependent ferroptosis escape pathwayRedox BiolApril 20, 2026Zitong Zhao, Ge Ge, Menghan Gong, Yi Liu, Liying Ma, Dongdong Zhang, Li Sun, Yongmei SongPierce Magnetic ChIP Kit (Thermo Scientific™, Shanghai, China). Chromatin was fragmented using a Covaris E220 Focused-ultrasonicator (Covaris, Woburn, MA, USA). Immunoprecipitation was conducted with anti-c-JUN antibody (Abcam, Cambridge,

(6) Genome-Wide Association Study Suggests rrp44 Is a Key Regulator of Growth Traits in Channel Catfish (Ictalurus punctatus)Curr Issues Mol BiolApril 18, 2026Shiyong Zhang, Hongyan Liu, Yongqiang Duan, Minghua Wang, Xiaohui Chenng/µL; OD260/OD280 = 1.8~2.0. The genomic DNA of each sample was then fragmented using the Covaris E220 focused ultrasonicator (Covaris, Brighton, UK) into fragments ranging from 50 to 800 bp. Fragments ranging from 100 to 300

(7) Combination Therapy with Copanlisib and Niraparib in Patients with Recurrent Endometrial and Ovarian Cancer (COPANIRA): Efficacy, Toxicity, and Translational InsightsClinical Cancer ResearchApril 1, 2026Allison L. Brodsky, Amma Asare, Bryan Fellman, Kristin Anderson, Jun Yao, Sanghoon Lee, Hai Tran, Xun Xu, Jinsong Liu, Joseph Celestino, et al.RRID:SCR_013575). Up to 200 ng of each gDNA sample, based on PicoGreen quantification, was sheared with the E220 Focused-ultrasonicator (Covaris, RRID:SCR_019817) under the following conditions: peak incident power 200, duty cycle 25%,

(8) FOXA1 loss drives basal/squamous de-differentiation of prostate cancer and induces an immunosuppressive tumor microenvironmentNature CommunicationsMarch 28, 2026Lourdes Brea, Hongshun Shi, Viriya Keo, Jing Huang, Liu Peng, Qi Chu, Wanqing Xie, Yinghua Xie, Sambhavi Senthil, Matthew T. Breneman, et al.was quenched with 0.125 M glycine for 5 min at room temperature. Chromatin was fragmented using an E220 focused ultrasonicator (Covaris), 50 ng of Drosophila chromatin was added to each sample as spike-in DNA, and samples were

(9) Haplotype-resolved reconstruction and functional interrogation of cancer karyotypesCell SystemsMarch 18, 2026Gregory J. Brunette, Richard W. Tourdot, Jinyu Wang, Darawalee Wangsa, David Pellman, Cheng-Zhong Zhangtreatment, the DNA sample was purified by AMPure XP beads, followed by fragmentation using Covaris E220 focused ultrasonicator. After end repair, adapter ligation and USER digestion, DNA was purified by AMPure XP beads, and then

(10) A mouse model of autosomal dominant spastic ataxia and myopathy caused by a mutation in Tuba4abioRxivMarch 9, 2026Timothy J. Hines, Jonathan R. Funke, Samia L. Pratt, Alaura D. Rice, Jeffery L. Twiss, Robert W. Burgessaccording to the manufactures’ protocols. Briefly, the protocol entails shearing the DNA using the E220 Focused-ultrasonicator (Covaris) targeting 200 bp, ligating Illumina-specific adapters, and PCR amplification. The amplified

(11) Mechanisms of gene regulation by SRCAP and H2A.ZNature CommunicationsMarch 6, 2026Armelle Tollenaere, Enes Ugur, Susanna Dalla Longa, Cédric Deluz, Devin Assenheimer, J. Christof M. Gebhardt, Heinrich Leonhardt, David M. Sutercycle, 140 W, 200 cycles or for sorted samples for 10 min at 10% duty, 75 W, 200 cycles on a Covaris E220 focused ultrasonicator. ChIPs were then performed using the ChIP-IT High Sensitivity kit (Active motif, #53040), following

(12) Integrated Histology and Molecular Profiling of Postmortem Human Auditory and Vestibular Organs via a Poly (Methyl Methacrylate)-Based WorkflowJournal of the Association for Research in OtolaryngologyFebruary 23, 2026David Bächinger, Brock Peyton, Jacqueline Neubauer, Anbuselvan Dharmarajan, MengYu Zhu, Jennifer T. O’Malley, Venus Kallupurackal, Steven Senese, Alison Brown, Sabina Wunderlin, et al.samples TV 1–6 and CL 1–3, genomic DNA was first sheared using a Covaris E220 focused ultrasonicator (#500239, Covaris, Woburn, MA). Libraries were then prepared with the NEBNext® Ultra™ II DNA Library Prep Kit

(13) Appropriate normalization is critical to improve reproducibility of tissue ChIP-seqSerican Journal of MedicineFebruary 9, 2026Qi Chu, Liu Peng, Lourdes Brea, Viriya Keo, Xiaodong Lu, Jindan Yu, Jonathan C. ZhaoCrosslinking was quenched with 0.125 M glycine for 5min at room temperature. Chromatin was fragmented using an E220 focused ultrasonicator (Covaris), 62.5 ng of Drosophila chromatin was added to each sample as spike-in DNA, and samples were

(14) Combination Therapy with Copanlisib and Niraparib in Patients with Recurrent Endometrial and Ovarian Cancer (COPANIRA): Efficacy, Toxicity, and Translational InsightsClin Cancer ResJanuary 13, 2026Allison L. Brodsky, Amma Asare, Bryan Fellman, Kristin Anderson, Jun Yao, Sanghoon Lee, Hai Tran, Xun Xu, Jinsong Liu, Joseph Celestino, et al.RRID:SCR_013575). Up to 200 ng of each gDNA sample, based on PicoGreen quantification, was sheared with the E220 Focused-ultrasonicator (Covaris, RRID:SCR_019817) under the following conditions: peak incident power 200, duty cycle 25%,

(15) FOXP3 expression depends on cell-type-specific cis-regulatory elements and transcription factor circuitryImmunityJanuary 13, 2026Jennifer M. Umhoefer, Maya M. Arce, Sivakanthan Kasinathan, Sean Whalen, Rama Dajani, Sanjana Subramanya, Laine Goudy, Julia A. Belk, Royce Zhou, Minh T. N. Pham, et al.0.5X protease inhibitor cocktail (PIC, Sigma, catalog no. P8340). Chromatin was sheared on a Covaris E220 focused ultrasonicator using 1 mL milliTubes (Covaris, catalog no. 520128) with 140W peak incident power, 5% duty factor, 200

(16) Senescence-driven molecular subtyping in pancreatic cancer: a multi-omics framework for precision medicineBMC CancerDecember 15, 2025Meilong Shi, Penghao Li, Bo Li, Huan Wang, Xiaoyi Yin, Xiaohan Shi, Suizhi Gao, Yikai Li, Chuanqi Teng, Shuai Yuan, et al.(Agilent Technologies, Santa Clara, USA) was used for target capture. DNA was fragmented to 350 bp using a Covaris E220 Focused-ultrasonicator (Covaris, Brighton, UK), followed by end-repair, A-tailing, and ligation of indexed adapters (Illumina,

(17) INSM1 governs a neuronal progenitor state that drives glioblastoma in a human stem cell modelNat CommunDecember 7, 2025Patrick A. DeSouza, Matthew Ishahak, Xuan Qu, Colin McCornack, Devi Annamalai, Diane D. Mao, Rajanikanth Vangipurapu, Yiwei Fu, Alexandre T. Vessoni, Ryan T. Cleary, et al.sonication to shear chromatin fragments to an average size between 200 bp and 500 bp on the Covaris E220 focused-ultrasonicator (peak power = 140, cycles/burst = 200, duty factor= 20, duration = 300 s). Fragmented chromatin was

(18) Exploitation of phage Carin-5's own DNA polymerase to sequence its T-hypermodified genomeSci RepDecember 5, 2025Rémi Sieskind, Sophia Missoury, Frédéric Bonhomme, Pauline Nogaret, Soizick Lucas-Staat, Marc Monot, Mikael Skurnik, Anne-Claire Baudoux, Marc DelarueIllumina TruSeq DNA UDI indexes. Genomic DNA was fragmented into ~ 350 bp fragments using a Covaris E220 focused ultrasonicator. After end repair, index-containing adapters were ligated. The libraries were sequenced on an Illumina

(19) Mutation profiling in differential diagnosis between TdT ‐positive high‐grade/large B‐cell lymphoma and B‐lymphoblastic leukaemia/lymphomaThe Journal of PathologyDecember 1, 2025Maria‐Myrsini Tzioni, Francesco Cucco, Lívia Rásó‐Barnett, Zi Chen, Andrew Wotherspoon, Katrin S Kurz, Ewelina Madej, Jasmine Makker, Anna E Strazda, Fang Guo, et al.genes investigated. A total of 80–200 ng of FFPE tissue‐derived DNA was fragmented using the Covaris E220 Focused Ultrasonicator (Covaris, Brighton, UK). For each DNA sample, an indexed library was prepared with the xGen™ UDI‐UMI

(20) Protocol for double-crosslinking ChIP-seq to improve data quality and enhance detection of challenging chromatin targetsSTAR ProtocolsDecember 1, 2025Yu Bao, Roberta Cacioppo, Alastair Crisp, Ivan Shlamovitz, Julian E. Sale, Ana Tufegdzic Vidakovic, Stephin J. Vervoort, Andrew Zellerultrasonicator settings and tube layoutScreenshot of the SonoLab software interface used to program the Covaris E220 focused-ultrasonicator. The sample tube layout in a 24-tube rack is shown on the left (outlined in red). On the right, the

Be the First to Write a Review!