E220 Focused-ultrasonicator from Covaris LLC

Product Specs
ItemE220 Focused-ultrasonicator
CompanyCovaris LLC
Price Get Pricing Get Quote for this Product
Catalog Number500239
Dimensions(WxDxH) 24 x 30 x 19 in. (61cm x 76cm x 48 cm)
ApplicationsDNA/RNA/Chromatin Shearing:DNA/RNA/Total NA/Protein Extraction from FFPE:cfDNA Extraction from Plasma:DNA Extraction from Dried Blood Spots:Cell lysis: mammalian, bacterial, yeast:Tissue lysis and extraction
Regulatory StatusCE, ETL Mark (for Product Safety), WEEE
MethodAcoustic Energy (Ultrasonic)
Sample VesslesAll AFA Tubes
QuantityEA
Electrical Requirements100-240 VAC 500 VA, 50-60 Hz
TemperatureAmbient temperature: 19° C to 25° C (66° F to 77° F) Relative humidity: 30% to 70%
Volume15 to 130 µl
ComponentsmicroTUBE 8-strip (rack required):microTUBE 96 plate

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Covaris LLC

14 Gill St, Unit H

Woburn, Massachusetts 01801-1721

United States

Phone: +1 781-932-3959
Fax: +1 781-932-8705

Email: info@covaris.com

Company Profile

Website: www.covaris.com

(1) Redox-triggered USP18 confers cisplatin resistance in ovarian cancer by selectively activating a non-canonical FSP1-dependent ferroptosis escape pathwayRedox BiolApril 20, 2026Zitong Zhao, Ge Ge, Menghan Gong, Yi Liu, Liying Ma, Dongdong Zhang, Li Sun, Yongmei SongPierce Magnetic ChIP Kit (Thermo Scientific™, Shanghai, China). Chromatin was fragmented using a Covaris E220 Focused-ultrasonicator (Covaris, Woburn, MA, USA). Immunoprecipitation was conducted with anti-c-JUN antibody (Abcam, Cambridge,

(2) Genome-Wide Association Study Suggests rrp44 Is a Key Regulator of Growth Traits in Channel Catfish (Ictalurus punctatus)Curr Issues Mol BiolApril 18, 2026Shiyong Zhang, Hongyan Liu, Yongqiang Duan, Minghua Wang, Xiaohui Chenng/µL; OD260/OD280 = 1.8~2.0. The genomic DNA of each sample was then fragmented using the Covaris E220 focused ultrasonicator (Covaris, Brighton, UK) into fragments ranging from 50 to 800 bp. Fragments ranging from 100 to 300

(3) Combination Therapy with Copanlisib and Niraparib in Patients with Recurrent Endometrial and Ovarian Cancer (COPANIRA): Efficacy, Toxicity, and Translational InsightsClinical Cancer ResearchApril 1, 2026Allison L. Brodsky, Amma Asare, Bryan Fellman, Kristin Anderson, Jun Yao, Sanghoon Lee, Hai Tran, Xun Xu, Jinsong Liu, Joseph Celestino, et al.RRID:SCR_013575). Up to 200 ng of each gDNA sample, based on PicoGreen quantification, was sheared with the E220 Focused-ultrasonicator (Covaris, RRID:SCR_019817) under the following conditions: peak incident power 200, duty cycle 25%,

(4) Haplotype-resolved reconstruction and functional interrogation of cancer karyotypesCell SystemsMarch 18, 2026Gregory J. Brunette, Richard W. Tourdot, Jinyu Wang, Darawalee Wangsa, David Pellman, Cheng-Zhong Zhangtreatment, the DNA sample was purified by AMPure XP beads, followed by fragmentation using Covaris E220 focused ultrasonicator. After end repair, adapter ligation and USER digestion, DNA was purified by AMPure XP beads, and then

(5) A mouse model of autosomal dominant spastic ataxia and myopathy caused by a mutation in Tuba4abioRxivMarch 9, 2026Timothy J. Hines, Jonathan R. Funke, Samia L. Pratt, Alaura D. Rice, Jeffery L. Twiss, Robert W. Burgessaccording to the manufactures’ protocols. Briefly, the protocol entails shearing the DNA using the E220 Focused-ultrasonicator (Covaris) targeting 200 bp, ligating Illumina-specific adapters, and PCR amplification. The amplified

(6) Mechanisms of gene regulation by SRCAP and H2A.ZNature CommunicationsMarch 6, 2026Armelle Tollenaere, Enes Ugur, Susanna Dalla Longa, Cédric Deluz, Devin Assenheimer, J. Christof M. Gebhardt, Heinrich Leonhardt, David M. Sutercycle, 140 W, 200 cycles or for sorted samples for 10 min at 10% duty, 75 W, 200 cycles on a Covaris E220 focused ultrasonicator. ChIPs were then performed using the ChIP-IT High Sensitivity kit (Active motif, #53040), following

(7) Integrated Histology and Molecular Profiling of Postmortem Human Auditory and Vestibular Organs via a Poly (Methyl Methacrylate)-Based WorkflowJournal of the Association for Research in OtolaryngologyFebruary 23, 2026David Bächinger, Brock Peyton, Jacqueline Neubauer, Anbuselvan Dharmarajan, MengYu Zhu, Jennifer T. O’Malley, Venus Kallupurackal, Steven Senese, Alison Brown, Sabina Wunderlin, et al.samples TV 1–6 and CL 1–3, genomic DNA was first sheared using a Covaris E220 focused ultrasonicator (#500239, Covaris, Woburn, MA). Libraries were then prepared with the NEBNext® Ultra™ II DNA Library Prep Kit

(8) Appropriate normalization is critical to improve reproducibility of tissue ChIP-seqSerican Journal of MedicineFebruary 9, 2026Qi Chu, Liu Peng, Lourdes Brea, Viriya Keo, Xiaodong Lu, Jindan Yu, Jonathan C. ZhaoCrosslinking was quenched with 0.125 M glycine for 5min at room temperature. Chromatin was fragmented using an E220 focused ultrasonicator (Covaris), 62.5 ng of Drosophila chromatin was added to each sample as spike-in DNA, and samples were

(9) FOXP3 expression depends on cell-type-specific cis-regulatory elements and transcription factor circuitryImmunityJanuary 13, 2026Jennifer M. Umhoefer, Maya M. Arce, Sivakanthan Kasinathan, Sean Whalen, Rama Dajani, Sanjana Subramanya, Laine Goudy, Julia A. Belk, Royce Zhou, Minh T. N. Pham, et al.0.5X protease inhibitor cocktail (PIC, Sigma, catalog no. P8340). Chromatin was sheared on a Covaris E220 focused ultrasonicator using 1 mL milliTubes (Covaris, catalog no. 520128) with 140W peak incident power, 5% duty factor, 200

(10) Senescence-driven molecular subtyping in pancreatic cancer: a multi-omics framework for precision medicineBMC CancerDecember 15, 2025Meilong Shi, Penghao Li, Bo Li, Huan Wang, Xiaoyi Yin, Xiaohan Shi, Suizhi Gao, Yikai Li, Chuanqi Teng, Shuai Yuan, et al.(Agilent Technologies, Santa Clara, USA) was used for target capture. DNA was fragmented to 350 bp using a Covaris E220 Focused-ultrasonicator (Covaris, Brighton, UK), followed by end-repair, A-tailing, and ligation of indexed adapters (Illumina,

(11) INSM1 governs a neuronal progenitor state that drives glioblastoma in a human stem cell modelNat CommunDecember 7, 2025Patrick A. DeSouza, Matthew Ishahak, Xuan Qu, Colin McCornack, Devi Annamalai, Diane D. Mao, Rajanikanth Vangipurapu, Yiwei Fu, Alexandre T. Vessoni, Ryan T. Cleary, et al.sonication to shear chromatin fragments to an average size between 200 bp and 500 bp on the Covaris E220 focused-ultrasonicator (peak power = 140, cycles/burst = 200, duty factor= 20, duration = 300 s). Fragmented chromatin was

(12) Exploitation of phage Carin-5's own DNA polymerase to sequence its T-hypermodified genomeSci RepDecember 5, 2025Rémi Sieskind, Sophia Missoury, Frédéric Bonhomme, Pauline Nogaret, Soizick Lucas-Staat, Marc Monot, Mikael Skurnik, Anne-Claire Baudoux, Marc DelarueIllumina TruSeq DNA UDI indexes. Genomic DNA was fragmented into ~ 350 bp fragments using a Covaris E220 focused ultrasonicator. After end repair, index-containing adapters were ligated. The libraries were sequenced on an Illumina

(13) Population Genomics Reveals Local Adaptation Related to Temperature Variation in Two Stream Frog Species: Implications for Vulnerability to Climate WarmingMolecular EcologyDecember 1, 2025Brenna R. Forester, Amanda S. Cicchino, Alisha A. Shah, Austin B. Mudd, Eric C. Anderson, Jessen V. Bredeson, Andrew J. Crawford, Jason B. Dunham, Cameron K. Ghalambor, Erin L. Landguth, et al.quantified samples as for whole genome resequencing samples. DNA was sheared to ~200 bp using a Covaris E220 focused-ultrasonicator at the University of California-Davis DNA Technologies Core. We used 230 –1000 ng (average ~900 ng)

(14) Mutation profiling in differential diagnosis between TdT ‐positive high‐grade/large B‐cell lymphoma and B‐lymphoblastic leukaemia/lymphomaThe Journal of PathologyDecember 1, 2025Maria‐Myrsini Tzioni, Francesco Cucco, Lívia Rásó‐Barnett, Zi Chen, Andrew Wotherspoon, Katrin S Kurz, Ewelina Madej, Jasmine Makker, Anna E Strazda, Fang Guo, et al.genes investigated. A total of 80–200 ng of FFPE tissue‐derived DNA was fragmented using the Covaris E220 Focused Ultrasonicator (Covaris, Brighton, UK). For each DNA sample, an indexed library was prepared with the xGen™ UDI‐UMI

(15) Protocol for double-crosslinking ChIP-seq to improve data quality and enhance detection of challenging chromatin targetsSTAR ProtocolsDecember 1, 2025Yu Bao, Roberta Cacioppo, Alastair Crisp, Ivan Shlamovitz, Julian E. Sale, Ana Tufegdzic Vidakovic, Stephin J. Vervoort, Andrew Zellerultrasonicator settings and tube layoutScreenshot of the SonoLab software interface used to program the Covaris E220 focused-ultrasonicator. The sample tube layout in a 24-tube rack is shown on the left (outlined in red). On the right, the

(16) Draft genome sequence of Bradyrhizobium sp. 33ap4 associated with effective root nodulation in pigeon pea ( Cajanus cajan )Microbiology Resource AnnouncementsNovember 13, 2025Francina Lebogang Bopape, Ahmed Idris Hassen, Eastonce Tendayi Gwataaccessed on 23 May 2025. For the draft genome sequencing, the extracted DNA was fragmented using a Covaris E220 Focused-Ultrasonicator (Thermo Fisher Scientific), followed by DNA library preparation using the MGIEasy Universal DNA Library Prep

(17) Stable clonal contribution of lineage-restricted stem cells to human hematopoiesisNature GeneticsNovember 11, 2025Tetsuichi Yoshizato, Christer Nilsson, Francesca Grasso, Kari Högstrand, Stefania Mazzi, Axel Winroth, Madeleine Lehander, Indira Barbosa, Gunilla Waldin, Teresa Mortera-Blanco, et al.individuals with CH10,18,23,36,51,52. Briefly, 200 ng (equivalent to 30,000 cells) of DNA fragmented with an E220 Focused-ultrasonicator (Covaris) was subjected to ECTS library preparation using a KAPA HyperPrep Kit (Kapa Biosystems), xGen

(18) Development of genomic markers for monitoring and research on plethodontid salamandersPLOS OneNovember 6, 2025Benjamin M. Fitzpatrick, Kara S. Jones, Aaron W. Aunins, Michael S. Eackles, David C. Kazyak1X TE buffer for mechanical shearing to a target mean fragment distribution of 250 bp with a Covaris E220 Focused Ultrasonicator. Settings for the Covaris were Peak incident power (W) 75, duty factor 10%, cycles per burst 200, and

(19) Long-Term Effects of Radiation Therapy on Cerebral Microvessel Proteome: A Six Month Post-Exposure AnalysisACS OmegaNovember 4, 2025Vikram Subramanian, Denise Juhr, Piero Giansanti, Isabella M. Grumbachbreak down the tissue, the samples were lysed using a Covaris E220 focused ultrasonicator (Covaris #500239) with following parameters. The instrument parameters for shearing were as follows: water level set

(20) Mapping the Telomeric 3D Interactome with Telomere-C Reveals Repetitive Element Hubs Associated with Telomere MaintenancebioRxivOctober 14, 2025Yi-An Chen, Ogechukwu Mbegbu, Noelle H. Fukushima, T. Rhyker Ranallo-Benavidez, Tianpeng Zhang, Floris P. Barthelbuffer (Qiagen, 19086), transferred to a microTUBE (Covaris PN 520045), and sonicated using a Covaris E220 Focused-Ultrasonicator with the following parameter settings: 105W Peak Incident Power, 2% Duty Factor, 200 Cycles per burst,

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