FongCyte™ Flow Cytometer from Challenbio

Product Specs
ItemFongCyte™ Flow Cytometer
CompanyChallenbio
Price Inquire
Catalog NumberFongCyte
Lasers1 to 3 lasers
Excitation405 nm, 488 nm, 638 nm
Parameters14 fluorescence channels
CapacityTubes (5 mL, 1.5 mL, 2 mL), Microplates (96-well)

(1) Clemizole inhibits CrtN-driven staphyloxanthin biosynthesis in Staphylococcus aureus to enhance host immune clearanceCommunications BiologyFebruary 25, 2026Hangqian Yu, Kaiyao Zhang, Jinli Ge, Kelong Ma, Shizhen Ma, Hongtao Liu, Qian Li, Jiazhang Qiuand Ly6G-PE (BioLegend) for 30 min on ice. Data were acquired via a flow cytometer and analyzed with FongCyte (Challenbio, China). Neutrophils were identified as Ly6G⁺CD11b⁺ populations after the exclusion of debris

(2) Multi-omics insights into Shenling Baizhu Powder’s amelioration of murine asthma through gut microbiota and Glutamine-GLS1 pathwayScientific ReportsFebruary 4, 2026Yuning Zeng, Hui Qi, Weijian Guo, Xueyi Tan, Bo Huang, Ruihui Hu, Xueren Ouyangtissue of mice were prepared for further flow cytometry analysis. Flow cytometry was assessed utilizing FongCyte C2080 (Challenbio, CN). The source of reagents employed for flow cytometric analysis in this study included

(3) Enhancing cancer susceptibility to disulfidptosis by inducing cell cycle arrest and impairing DNA repairTheranosticsJanuary 1, 2026Jing Lin, Xueli Yang, Cizhong Jiang, Xiaoguang Liu, Jiejun Shipropidium iodide (PI) in pre-cold PBS. The PI-positive (dead) cells were analyzed by a follow cytometer (FongCyte, Challenbio). Western blotting Western blotting was conducted as previously described 3. Briefly,

(4) Ecm19 coordinates mitochondrial fission and cellular redox homeostasisRedox ReportDecember 31, 2025Tiantian Chen, Sisi Lei, Haomin Qiu, Dan Zhao, Cuimei Hu, Yi Li, Xueya Zhao, Tiantian Tang, Jiaxin Deng, Zengyi Huang, et al.localization of DiOC6 was visualized via confocal microscopy. Fluorescence intensity was quantified using a FongCyte™ flow cytometer (Challenbio, China), with data analyzed in FlowJo 10.8.1. 2.5. RNA extraction, cDNA synthesis, and

(5) MRPL37 promotes hepatocellular carcinoma progression through modulating mitochondrial energy metabolismiScienceDecember 19, 2025Yigan Zhang, Minjie Chen, Huidi Li, Hao Deng, Shuwen Chen, Jiaxin Ni, Junjie Hu, Sixian Lei, Linsheng Huang, Shuangsuo Dang, et al.Sofware,https://www.graphpad.com/ ,, Other,Other,Other ,, Laser scanning confocal focus microscope,NOVEL,NCF-1000 Flow cytometer,Challenbio,FongCyte™3 IHC h-score analysis,PathologyProfiler,V0.1.4

(6) Renal clearable CRISPR nanosensor targeting mitochondrial DNA mutation for noninvasive monitoring of tumor progression and metastasisScience AdvancesNovember 21, 2025Yanan Li, Yonghua Wu, Ziyue Zheng, Yige Wu, Yongyuan Zhang, Jingjing Zhang, Fenglei Quan, Wen Zhao, Ru Xu, Yiwei Li, et al.MitoTracker Red, respectively. Cells were fluorescently imaged by CLSM. Flow cytometry (Challenbio, FongCyte, China) was then used for analysis. Examination of fluorescent signal generation by CasUber probe for

(7) FLT3L combined with GM-CSF induced dendritic cells drive broad tumor-specific CD8+ T cell responses and remodel the tumor microenvironment to enhance anti-tumor efficacyFrontiers in ImmunologySeptember 4, 2025Qian Zheng, Jiajie Zhang, He Sui, Yu Sun, Ningning Lv, Lin Liu, Ming Qu, Jiateng Tan, Bin Zhang, Zhanhao Mowere incubated with the antibody mixture for 30 min at 4°C. Flow cytometry was performed using the FongCyte™ instrument (Challenbio), and data were analyzed with FlowJo v10.1 (RRID:SCR_008520). To set up fluorescence

(8) Microglial BDNF modulates arketamine's antidepressant-like effects through cortico-accumbal pathwaysSci AdvJuly 11, 2025Lujuan He, Xuenan Wang, Shilin Luo, Nan Jiang, Yu Yan, Min Xie, Yueyue Chen, Chun Yang, Wei Yao, Kenji Hashimoto, et al.Allophycocyanin (APC)-conjugated anti-CD11b antibody (BioLegend, #101211). Data were acquired using a FongCyte 2 Laser 6-Color Flow Cytometer C2060 (Challenbio, Beijing, China) and analyzed using FlowJo software. Patch-clamp

(9) Copper-Based Metal-Organic Framework Nanoplatform for miRNA Delivery: Synergistic Antitumor TherapyInt J NanomedicineJuly 3, 2025Maopu Tu, Xiaoyu Deng, Bin Lai, Jiao Liu, Yingzhou Tao, Shaohua Xu, Xiaodong Li, Xiaoqiang Niu, Zhixin Li, Jiahui Huang, et al.FAM-miR-4521 or FAM-miR-4521@MOF-199 for 8 h, washed with PBS, and analyzed by flow cytometry (Challenbio Fongcyte C2060, china) to quantify fluorescence intensity. In addition, after HGC-27 cells were treated with

(10) Lactobacillus acidophilus extracellular vesicles-coated UiO-66-NH2@siRNA nanoparticles for ulcerative colitis targeted gene therapy and gut microbiota modulationJ NanobiotechnologyApril 17, 2025Chenyang Cui, Jiaze Tang, Jie Chen, Beining Zhang, Ruonan Li, Qiang Zhang, Chunjing Qiu, Rongchen Chen, Geng Min, Zhaowei Sun, et al.suspension (approximately 10,000 cells) was transferred to a flow cytometry tube (BD, USA) for analysis. The FongCyte™ flow cytometer (Challenbio, Beijing, China) was used to assess the fluorescence intensity utilizing the FITC channel

(11) Locoregional Immune Checkpoint Blockade and Remodeling of Lymph Nodes by Engineered Dendritic Cell‐Derived Exosomes for Suppressing Tumor Progression and MetastasisAdvanced ScienceApril 3, 2025Yizhen Wang, Xiaomin Guo, Jingya Qin, Yifan Xue, Peng Zhang, Yadong Liu, Moyang Chen, Guanghao Zhu, Xinqiu Song, Lili Cheng, et al.instructions. The fluorescent signals of cell samples were detected by flow cytometer (Challenbio, FongCyte, China) and the data were analyzed with its accompanying software and FlowJo software. Accumulation

(12) Recent Developments (After 2020) in Flow Cytometry Worldwide and Within ChinaBiosensors (Basel)March 2, 2025Xinyue Du, Xiao Chen, Chiyuan Gao, Junbo Wang, Xiaoye Huo, Jian ChenChallenbio in 2022 can be equipped with three lasers and fourteen colors at most, and the latest updated FongCyte™ series in 2024 can be configured with up to four lasers. However, spectral overlap between fluorochromes

(13) Acute hypoxia modulate macrophage phenotype accompanied with transcriptome re-programming and metabolic re-modelingFrontiers in immunologyJanuary 1, 2025Sun B, Long Y, Xu G, Chen J, Wu G, Liu B, Gao Y.and Brilliant Violet 650 anti-mouse CD86 (105035, Biolegend). Flow cytometry was performed using a Fongcyte™ S flow cytometer (Challenbio, Beijing), and data were analyzed using the manufacturer-provided ModelFlower

(14) Fatty acid metabolism prognostic signature predicts tumor immune microenvironment and immunotherapy, and identifies tumorigenic role of MOGAT2 in lung adenocarcinomaFrontiers in ImmunologyOctober 16, 2024Denggang Fu, Biyu Zhang, Wenyan Fan, Fanfan Zeng, Jueping Feng, Xin Wangemployed for apoptosis detection following manufacturer’s instructions. Finally, flow cytometry analysis (FongCyte, Challenbio, China) was conducted to assess apoptosis distribution. 2.13 Cell cycle assay MOGAT2 WT

(15) Creating a bionic scaffold via light-curing liquid crystal ink to reveal the role of osteoid-like microenvironment in osteogenesisBioactive MaterialsOctober 1, 2024Kun Liu, Lin Li, Yizhi Li, Yiting Luo, Zhaoyu Zhang, Wei Wen, Shan Ding, Yadong Huang, Mingxian Liu, Changren Zhou, et al.mineralization. Gene expression of cells cultured on various scaffolds was evaluated by real time PCR (RT-PCR, FongCyte, Challenbio). The primers sequences are summarized in Table S2. The RNase inhibitor is from East-Mab

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FongCyte™ Flow Cytometer

FongCyte™ Flow Cytometer from Challenbio

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FongCyte™ Flow Cytometer from Challenbio

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