ID7000™ Spectral Cell Analyzer from Sony Corporation

Product Specs
ItemID7000™ Spectral Cell Analyzer
CompanySony Corporation
Price Inquire
Catalog NumberID7000
Detection32-channels (PMT arrays); can be configured with 186 detectors to detect 44 colors or more
Throughput40,000 events per second
Lasers3 to 7 lasers
Excitation320 nm, 355 nm, 405 nm, 488 nm, 561 nm, 637 nm, 808 nm
Parameters186 fluorescence channels
CapacityTubes (5 mL), Microplates using autosampler (96-well, 384-well)
Data Throughput40,000 events/sec
Dimensions(WxDxH) 106 x 71.9 x 76 cm
Flow Rate33 to 200 µL/min

(1) A macrophage-mimetic nanocarrier co-loaded with geraniol and FK13-a1 for MRSA-induced acute lung injuryDrug DelivDecember 31, 2026Haonan Li, Binghong Xu, Junguang Liang, Tao Hu, Tingting Wu, Liqin Lai, Xuan Zou, Ziquan Lv, Xiangjie Yao, Xiaobao Jin, et al.CD4+ T cells, CD8+ T cells, neutrophils, monocyte/macrophages, and Tregs were detected using a Sony ID7000 flow cytometer. 2.22. Statistical analysis All data are presented asmean ± standard deviation (SD)

(2) Natural senolytic activity of Rhodiola rosea extract alleviates age-associated phenotypes via paraptosisiScienceMay 15, 2026Ryo Furuuchi, Yohko Yoshida, Goro Katsuumi, Takaaki Furihata, Yusuke Joki, Chieh-Lun Hsiao, Masayoshi Suda, Hana Saito, Tamano Kumazaki, Hidefumi Makabe, et al.following the manufacturers’ protocols. Stained cells were analyzed using the Spectral Cell Analyzer ID7000 (Sony), and the proportions of PI-positive cells were quantified. Systemic metabolic parameter For

(3) Nasal CD4+ tissue-resident memory T cells provide cross-protective immunity to influenzaJ Exp MedMay 4, 2026Nimitha R. Mathew, Romain Gailleton, Lydia Scharf, Karin Schön, Josue Enriquez, Hannes Axelsson, Anneli Strömberg, Nils Lycke, Mats Bemark, Ka-Wei Tang, et al.cells were washed and suspended in FACS buffer for data acquisition at BD LSRFortessa X-20 or Sony ID7000 spectral analyzer. Flow cytometry of murine cells All fluorochrome-conjugated antibodies are titrated

(4) CD163 and Tim-4 identify resident intestinal macrophages that are spatially regulated by TGF-βJ Exp MedMay 4, 2026Vignesh Jayaraman, Ian E. Prise, Verena Kästele, Sabrina Tamburrano, Kelly Wemyss, Hayley M. Bridgeman, Rufus H. Daw, Patrick Strangward, Alfie Sanderson, Sheena M. Cruickshank, et al.Scientific. Cell acquisition was performed on an LSRFortessa running FACSDIVA software (BD) or SONY ID7000 (SONY). For each intestinal sample, typically 10,000–20,000 macrophages were collected. Data were analyzed

(5) Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marroweLifeApril 27, 2026Jing-Xin Feng, Mei-Ting Yang, Lili Li, Caiyi C Li, Ferenc Livák, Jack Chen, Yongmei Zhao, Dunrui Wang, Avinash Bhandoola, Naomi Taylor, et al.acquired with BD FACSCanto-II, BD LSRFortessa, BD FACSymphony A5 (BD Biosciences), Sony SA3800 or Sony ID7000 cell analyzers. Cell sorting was performed with BD FACS Aria III, BD FACS Aria Fusion, or Sony SH800S

(6) Associations between food consumption with T cell activation and antibody responses following SARS-CoV-2 mRNA vaccinationGut Microbes ReportsMarch 30, 2026Hiroki Negishi, Gaku Nakato, Rie Kadowaki, Hiroki Kono, Mami Minakata, Ayumi Ichikawa, Takayuki Toshimitsu, Seiya Makino, Hiroshi Kano, Sho Nakamura, et al.780 (Invitrogen, USA). Stained cells were fixed with Cytofix/Cytoperm™ (BD) and acquired using the ID7000™ Spectral Cell Analyzer (Sony, Japan). Data were analyzed using FlowJo software. Flow cytometry gating strategy is detailed

(7) Circulating exhausted CD8+ effector memory cells differentiate immune checkpoint inhibitor-induced liver injury from other acute immune-mediated liver injuriesJ Immunother CancerMarch 27, 2026Stuart Astbury, Edmond Atallah, Jane I Grove, Amber G Bozward, Scott P Davies, Mark J Sheehan, Steven W Kumpf, Jessie Qian, Natalia M Krajewska, Grace E Wootton, et al.antibodies and matched isotype controls (online supplemental table 3) and data were acquired on an ID7000 spectral flow cytometer (Sony Biotechnology). Data were analyzed in FlowJo V.10 software. Fluorescence

(8) CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancerCell Death & DiseaseMarch 26, 2026Si Sun, Lin Huang, Yujia Ma, Zheng Wei, Mengna Zhu, Mengqing Chen, Feiquan Ying, Xiaoling Zhou, Ping Yang, Yiping Wen, et al.or 556547) according to the manufacturer’s instructions. Flow cytometry analysis was conducted on an ID7000 Spectral Cell Analyzer (Sony Biotechnology). Western blot Cells were harvested and lysed using the Mammalian Cell & Tissue

(9) Application of spectral flow cytometry for comprehensive detection of immune metabolism in patient-derived microsamplesCell Rep MethodsMarch 23, 2026Yang Bai, Yuqing Wang, Yicheng Fu, Zhengyang Guo, Zhaoyuan Liang, Liu Yang, Jiawei Ribaudo, Dan Liu, Yanfang Li, Ting Zhang, et al.probes for 30 min at 37°C. Cells were washed and then acquired by full spectrum flow cytometry (SONY ID7000). The working concentration of all fluorescent probes are listed in Table 1. Negative and positive controls

(10) Systematic profiling of SARS-CoV-2 structural protein-specific T cell epitopes in Omicron infections following inactivated vaccinationiScienceMarch 20, 2026Zhiqing Li, Mengmeng Cui, Jian Wu, Tianju Hu, Junyan Dan, Xiaosu Chen, Qicong Shen, Jin Hou, Zhongfang Wang, Yizhi Yu, et al.Cells were washed with Perm/Wash™ Buffer and acquired within 24 hours on BD LSRFortessa™ X-20 or Sony ID7000™ spectral cytometers. Flow cytometry data were analyzed using FlowJo™ v10.10.0 . Systematic high-throughput screening

(11) Flow cytometric evaluation of monoclonal antibodies for cross-reactivity with feline leukocytesFrontiers in Veterinary ScienceMarch 18, 2026H. Krüger, A. Moritz, B. Roshani, S. Neumanndiscarded, and the cells were resuspended in 150 μL PBS/5% BSA for analysis. Cells were acquired using the ID7000™ spectral analyzer (Sony Biotechnology, San Jose, CA, USA). Unmixing was calculated using single-stained

(12) The MEK1/2-IRF4 axis fosters T follicular helper cell differentiationand antitumor humoral immune responseJournal of Translational MedicineMarch 16, 2026Shuan Ran, Song Wang, Ran Li, Longyong Lai, Jizhang Yu, Xi Zhang, Yuan Li, Weicong Ye, Junjie Zong, Xiaohan Li, et al.(Biolegend, #406421) at 37 °C for 30 min. After staining as described above, the cells were processed with an ID7000 flow cytometer (Sony) or an LSR Fortessa X-20 flow cytometer (BD Biosciences), and the data were analyzed

(13) Ageing promotes metastasis via activation of the integrated stress responseNatureMarch 11, 2026Angana A. H. Patel, Jozefina J. Dzanan, Kevin X. Ali, Ella A. Eklund, Samantha W. Alvarez, Dorota Raj, Martin Dankis, Ilayda Altinönder, Maria Schwarz, Kristell Le Gal, et al.suspensions were prepared from spheroids on days 0, 2, 4, 6 and 8, flow cytometry data were collected on Sony ID7000 Spectral Cell Analyzer and analysed using Flow Jo software v.10.8.1. Lentiviral production and transduction Lentiviruses

(14) Multifunctional copper-based nanoparticles potentiate colorectal cancer immunotherapy via synergistic metabolic remodeling and cGAS-STING pathway activationJournal of NanobiotechnologyMarch 8, 2026Maopu Tu, Xiaoyu Deng, Zhiyong Zhou, Xiaodong Li, Shengxun Mao, Bin Lai, Lisong Pang, Qilin Zhong, Jiaqing Cao, Haipeng Liu, et al.(CLSM, Leica Stellaris STED). Parallel samples were analyzed by flow cytometry (SONY Group Corporation ID7000). For intracellular copper analysis, cells were collected after 6 h incubation with formulations, washed

(15) NCOA2 promotes the return of hematopoietic stem cells to quiescence after irradiation stress by regulating FOXO3a-dependent mitophagyHemasphereMarch 7, 2026Fang Chen, Naicheng Chen, Linjie Wang, Hao Zeng, Yukai Lu, Yanying Liu, Jing Chen, Yang Xu, Mo Chen, Song Wang, et al.Alexa Fluor 488 (#A‐11034, Invitrogen, Carlsbad, CA, USA). Flow cytometric analysis was performed by ID7000 (Sony Biotechnology, Japan), FACSverse (BD Biosciences), or FACSymphonyTM A1 (BD Biosciences), and the

(16) aGPCR-HEK: A Stable High-Expression Inducible Mammalian Cell Expression System for Adhesion GPCR Structural Biology ApplicationsBIO-PROTOCOLMarch 5, 2026David M. Favara, Christopher G. TateInvitrogen Bigfoot Cell Sorter (Invitrogen, catalog number: PL00304) 9. Flow cytometer (Sony, model: ID7000 Spectral Cell Analyzer) 10. FSEC system: Shimadzu HPLC system comprising the following components: Shimadzu Degasser Module

(17) Purification of the Active-State G Protein-Coupled Receptor ADGRL4 for Cryo-Electron Microscopy Using a Modular Tag System and a Tethered mini-G_qBIO-PROTOCOLMarch 5, 2026David M. Favara, Christopher G. Tate300 mesh Au grids (Quantifoil, catalog number: Q350AR13A) Equipment 1. Flow cytometer (Sony, model: ID7000 Spectral Cell Analyzer) 2. Temperature-controlled floor-standing centrifuge for 1 L bottles (Beckman Coulter, Avanti JXN-26

(18) IgE to cyclophilin Bet v 7 triggers mast cell activation and mediates cross-reactivity with Ara h 18 in children with seasonal allergic rhinitisPediatr Allergy ImmunolMarch 4, 2026Wilhelm Sponholz, Carolin Steinert, Francesco Monnati, Ekaterina Potapova, Jonas Lidholm, Lars Mattsson, Enrico Scala, Roberto Bernardini, Carlo Caffarelli, Antonella Casani, et al.DAPI prior to measurement. Flow cytometric measurements were performed on the spectral cell analyzer (ID7000, Sony) and analyzed using the gating strategy shown in Figure 1. MAT results were calculated as the

(19) CD70-Targeting CAR NK Cells Overcome BCMA Downregulation and Improve Survival in High-risk Multiple Myeloma ModelsBlood Cancer DiscoveryMarch 4, 2026Paul Lin, Sunil Acharya, Francia Reyes-Silva, Rafet Basar, Nadima Uprety, Luz Yurany Moreno Rueda, Pei Lin, April L. Gilbert, Pinaki P. Banerjee, Dexing Fang, et al.for flow cytometry are described in Supplementary Table S1. Multiparametric data were acquired using the ID7000 spectral flow cytometer (Sony) and analyzed with the Kaluza software (Beckman Coulter). Dot plots were

(20) Pharmacological inhibition of USP18 improves antibacterial responses and the intracellular control of Mycobacterium tuberculosis in macrophagesFront ImmunolMarch 4, 2026Qiao Zhang, Zhen Gong, Robert Schnell, Yi Zhong, Paul P. Geurink, Volker M. Lauschke, Jianping Xie, Stefano Gastaldello, Martin E. Rottenbergfixed with 4% formaldehyde (Sigma-Aldrich) at room temperature for 10 min. Data were acquired on a Sony ID7000 spectral cytometer and analyzed with FlowJo software (Tree star Inc., Ashland, OR). Cells within the

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ID7000™ Spectral Cell Analyzer

ID7000™ Spectral Cell Analyzer from Sony Corporation

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ID7000™ Spectral Cell Analyzer from Sony Corporation

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