NovoCyte Advanteon Flow Cytometer Systems from Agilent Technologies

Product Specs
ItemNovoCyte Advanteon Flow Cytometer Systems
CompanyAgilent Technologies
Price Get Pricing Get Quote for this Product
Catalog NumberNovoCyte Advanteon
DetectionFSC, SSC, with 30 fluorescent channels
Voltage100/115/230 VAC, 50 to 60 Hz
ApplicationsApoptosis Assay, Immunophenotyping, Cell Proliferation, Cytokine Detection, Intracellular Protein Detection, Cell Cycle Analysis
QuantityEA
Lasers1 to 3 lasers
Excitation405 nm (50 mW), 488 nm (60 mW), 561 nm (50 mW), 640 nm (40 mW)
Parameters23 parameters / 21 fluorescence channels
CapacityTubes, Microplates using autosampler (48-well, 96-well, 384-well)
Data Throughput100,000 events/sec
Dimensions(WxDxH) 85 × 46 × 48 cm

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Agilent Technologies

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Santa Clara, California 95051

United States

Phone: +1 877-424-4536

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Website: www.agilent.com

(1) Species-specific chlorine resistance and biofilm regulation by extracellular polymeric substances and quorum sensing in drinking water pipeline bacteriaApplied and Environmental MicrobiologyApril 22, 2026Jia Niu, Daogan Chen, Tian Lin, Shixuan Li, Jiayan Xu, Huacheng Xu, Xianhua Liu, Xiaochen Chen, Nan Wei, Kro Mengthen placed into sterile flow tubes. Finally, the number of bacteria was determined by flow cytometry (NovoCyte Advanteon BR, Agilent Technologies, Inc., USA) equipped with a 488 nm solid-state laser. Following the method

(2) Microenvironment-responsive injectable dynamic hydrogel for sequential antioxidant and tissue regeneration therapy of radiation-induced skin injury☆Bioact MaterApril 21, 2026Xiaorui Yu, Tingyu Yang, Zhongwu Bei, Jie Liu, Ying Song, Qiang Wang, Jianan Li, Shiyu Liang, Zhiyong Qianallophycocyanin (APC). After being washed twice with PBS, the samples were analyzed using a flow cytometer (NovoCyte Advanteon, Agilent, USA). 2.24 Intracellular antioxidant assay HUVECs (1 × 104 cells per well) were seeded in

(3) Insufficient erythrocyte-derived S1P: A pathogenic driver and diagnostic biolipid for tumor progressioniScienceApril 17, 2026Zhenjiang Li, Weilun Huang, Zhouzhou Yao, Juncheng Wang, Wenhao Xiao, Qi Guo, Changhan Chen, Tingting Xie, Zhiyu Yang, Qingyang Liu, et al.light-protected conditions, with parallel isotype controls. Stained cells were analyzed using flow cytometry (NovoCyte Advanteon, Agilent, USA), and data were processed in FlowJo (Tree Star). Quantification and statistical analysis Data

(4) Multi-Omics Analyses Identify ANLN as a Prognostic Biomarker for Recurrence and Metastasis in Non-Small Cell Lung CancerGenes (Basel)April 15, 2026Haiwei Quan, Zhiguang Xu, Lizhen Huo, Zhibin Wangin the dark, followed by addition of 400 μL binding buffer. Samples were analyzed immediately using NovoCyte Advanteon VBR (Agilent, Santa Clara, CA, USA), collecting 10,000 events per sample, with three technical replicates.

(5) Lidocaine inhibits hepatocellular carcinoma cell proliferation, migration, and invasion through the downregulation of SLC6A3Sci RepApril 7, 2026Peiyang Li, Wulan Tong, Hong He, Hao Liu, Xi Yangcontrols and incubated for 5 min protected from light. Apoptotic cells were analyzed by flow cytometry (Novocyte Advanteon VBR, Agilent, USA). HCC cell invasion assay The invasive capacity of HCC cells was assessed using

(6) Ionizing radiation promotes lung injury by inducing ferroptosis-driven senescence in epithelial cells via NCOA4-mediated ferritinophagyRedox BiologyApril 1, 2026Xiaoying Qian, Yong Wang, Peng Kuang, Bingda Li, Ke Jiang, Anqi Wang, Kangping Xiong, Weiwei Hong, Biao Yu, Guizhen Qin, et al.assay kit (BestBio, BB-4105, China) following instructions [28]. Samples were measured using an Agilent NovoCyte Advanteon, and the acquired data were processed with FlowJo V10 software. The mitochondrial function of the cells

(7) hnRNPA1-SF3B3 interaction drives radioresistance in oral squamous cell carcinoma by modulating MARF1 alternative splicing isoformsJournal of Experimental & Clinical Cancer ResearchMarch 21, 2026Fan-tong Xia, Si-qi Cai, Jie-ying Yang, Jia Jiang, Yi-hong Hu, Huan Zhang, Meng-qi Yang, Xin Zhang, Dan Li, Yun-chang Liu, et al.After transfection of plasmids for 48 h, GFP-positive cells were measured by flow cytometry using a Novocyte Advanteon (Agilent Biosciences) flow cytometer. The flow cytometry gating strategy was performed as follows: single

(8) An Engineered Extracellular Vesicle With Enhanced Tumor and Lymph Nodes Targeting as siRNA Delivery System for Robust Tumor ImmunotherapyMedComm (2020)March 9, 2026Yusi Wang, Rui Zhang, Xuejing Zhou, Lin Tang, Die Hu, Yibing Zhang, Yuling Yang, Bailing Zhou, Li Yangsuspensions and stained with antibodies against CD45, CD11c, and CD103 for flow cytometric analysis using a NovoCyte Advanteon Dx flow cytometer (Agilent, USA). 4.6 In Vitro Uptake Efficiency BMDCs, BMDMs, and E.G7‐OVA cells

(9) MCC-135 Exerts Antiepileptic and Neuroprotective Effects by Downregulating NCX1 Expression to Decrease Intracellular Calcium Overload in the HippocampusCNS Neurosci TherMarch 2, 2026Chaoning Liu, Min He, Rida Li, Shouhuan Zheng, Lanfeng Sun, Chi Gong, Hengchang Qi, Xinran Qin, Xiaohang Gan, Fang Wang, et al.according to the manufacturer's instructions. Fluorescence intensity was measured using a flow cytometer (NovoCyte Advanteon, Agilent, China). The measurement value for each sample was the average fluorescence intensity of 10,000

(10) ACSBG1‑driven lipid reprogramming unveiled: A fatty acid metabolism‑associated prognostic model for lung adenocarcinomaOncology ReportsFebruary 27, 2026Panxin Hu, Anan Liunder light-protected conditions at 25°C. Flow cytometric quantification was conducted using an Agilent NovoCyte Advanteon Dx VBR system (IVD-CE certified; Agilent Technologies, Inc.) coupled with NovoExpress software (v1.6.2;

(11) Identification and Functional Validation of PTH2R as a Therapeutic Target in Lung AdenocarcinomaBiomedicinesFebruary 23, 2026Changmin Liu, Yongfu Wang, Wei Liu, Yizhen Yuan, Yajing Xue, Pengzhuo Tao, Dan Sun, Te Kian Keong, Shilin Chen, Chi Songminutes, the mixture was incubated in the dark. Finally, a flow cytometer was used for analysis (Agilent NovoCyte Advanteon, San Diego, CA, USA). 2.9. Wound Healing Assay The Cell Scratch Film (Beyotime, Boston, MA, USA) was

(12) CD47 blockade (ALX301) enhances immunoradiotherapy response in HPV negative head and neck squamous cell carcinomaPLOS OneFebruary 17, 2026Abdula Monther, Riyam Al-Msari, Robert Saddawi-Konefka, Santiago Fassardi, Cynthia Tang, Chad Philips, Prakriti Sen, Pardis Mohammadzadeh, Kelsey Decker, Sayuri Miyauchi, et al.Brilliant Stain Buffer (BD Biosciences). The cells were fixed with 2% paraformaldehyde and analyzed on Agilent NovoCyte Advanteon (Agilent Technologies) with standard lasers and optical filters. Data was analyzed using FlowJo (FlowJo,

(13) Expression Profile of Metabotropic Glutamate Receptors in Lung Adenocarcinoma: GRM5 and Validation of Its Targeting Drug CinchonineInternational Journal of Molecular SciencesFebruary 13, 2026Yajing Xue, Wei Liu, Yongfu Wang, Pengzhuo Tao, Yizhen Yuan, Changmin Liu, Shilin Chen, Chi Songcells were stained via the Annexin V-APC/DAPI (Elabscience, Wuhan, China) kit. Flow cytometry (Agilent NovoCyte Advanteon, San Diego, CA, USA) was used to detect and analyze the data. 4.12. Tango Ligand Activation Assay When

(14) Clinical efficacy and safety of drug-eluting bead transarterial chemoembolization combined with targeted therapy and immune checkpoint inhibitors in the treatment of intermediate-to-advanced hepatocellular carcinomaPakistan Journal of Medical SciencesFebruary 1, 2026Hao Lu, Yuanlong Zhou, Chen Liu, Zan Li, Zhiyuan Luoaccordance with the manufacturer’s instructions. The remaining portion was analyzed by flow cytometry (NovoCyte Advanteon, Agilent Technologies [China] Co., Ltd.) to determine the levels of CD3+, CD8+ and CD4+ T cells. Adverse

(15) Tailoring Polydopamine Nanoparticle Size Through Synthesis Conditions and Defining Their Antioxidant and Neuroprotective EfficacyInternational Journal of NanomedicineJanuary 27, 2026Qianqian Huang, Junbo Zou, Fei Luan, Yajun ShiHitachi, Shimadzu, Japan), fluorescence microscope (Thunder DMi8, Leica, Germany), flow cytometer (NovoCyte Advanteon, Agilent, USA), and cell incubator (CCL-170B-8, ESCO, Singapore) were used. Effect of Reaction Conditions

(16) Dual targeting of lipid metabolic reprogramming and immunosuppressive sentinel lymph nodes potentiates anti-metastatic therapy for triple negative breast cancerBioactive MaterialsJanuary 1, 2026Tingting He, Xuemei Liao, Shuangquan Gou, Genhua Liu, Qiaojian Duan, Zizhen Qin, Junli Yang, Yu Sun, Peng Zhao, Zhong Luo, et al.trypsinization before FCM analysis. FCM testing was stopped once gated populations reached 104 cells using a Novocyte Advanteon instrument (Agilent, America). Three parallel were set for each group. The results were analyzed with

(17) CCL19-armed recombinant influenza virus inhibited colorectal cancer growth by remodeling tumor micro-environmentiScienceDecember 19, 2025Xia Ou, Yunxin Xia, Zhongyue Fang, Kai Yang, Guangtao Yang, Junying Wang, Yiyong Duan, Xiahui Yang, Bing Yang, Ze Liu, et al.Table 2. Equipment The key instruments used in this study are as follows: Flow cytometer: Agilent NovoCyte Advanteon (1–3 Lasers) Flow Cytometer (Agilent Technologies, Santa Clara, CA, USA); Real-time cell analysis system:

(18) Medium dependent factors govern the functionality of engineered type III secretion systemsJournal of Biological EngineeringDecember 16, 2025SangKu Yi, Beom Seok Kim, Eunna Choi, Eun-Jin Lee, Juhyun KimTech, Korea). After incubation, the relative abundance of each strain was determined by flow cytometry (NovoCyte Advanteon, Agilent Technologies, USA) based on RFP and GFP fluorescence signals. Due to the bistable expression

(19) Anti-Cancer Effects of the Ethyl Acetate Fraction From Opuntia humifusa on Human Triple-Negative Breast Cancer CellsIntegr Cancer TherDecember 15, 2025Myung Chul Cha, Eonji Yeo, Dong Seok Leeadding 400 μL of Binding Buffer, samples were analyzed immediately using a flow cytometer (Agilent Novocyte Advanteon, CA, USA) equipped with NovoExpress™ software (Agilent Technologies, RRID: SCR_024676) for data acquisition

(20) Comparative study of analytical methods for assessing mRNA integrity and identification of functional mRNA oligomersSci RepDecember 10, 2025Perrine Lardellier, Camille Malburet, Marco Morani, Thibaut Willemin, Javier Rol-Moreno, Anouck Noir, Stéphanie Fertier-Prizzon, Marc Francois-Heudeantibody conjugated with Pacific Blue fluorochrome, and analyzed by flow cytometry. Data was acquired on a NovoCyte Advanteon using NovoExpress software (Agilent). Median fluorescence intensity and percentage of positive cells

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NovoCyte Advanteon Flow Cytometer Systems 1-3 Lasers

NovoCyte Advanteon Flow Cytometer Systems 1-3 Lasers

NovoCyte Advanteon Flow Cytometer Systems from Agilent Technologies

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NovoCyte Advanteon Flow Cytometer Systems from Agilent Technologies

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