MACSQuant® Analyzer 10 Flow Cytometer from Miltenyi Biotec

Product Specs
ItemMACSQuant^® Analyzer 10 Flow Cytometer
CompanyMiltenyi Biotec
Price Get Pricing Get Quote for this Product
Catalog Number130-196-434
Volume1µL min. / 25 µL recommended uptake volume
ApplicationsFlow Cytometry
Sample Type1.5/2.0/5.0/15/50 mL tubes or 24/48/96-well plates
ThroughputUp to 15,000 events per second
Abs / Exc405 nm (violet)
488 nm (blue)
640 nm (red)
DetectionFSC, SSC, 8 fluorescence channels
Voltage100 to 240 VAC, 50/60 Hz
QuantityEA
Dimensions(W x D x H* *adjustable touch screen) 669 x 400 x 394-553.5 mm
Regulatory StatusFor research use only
Speed<25 minutes per 96-well plate (5 µL measurement volume per well)

Referred value indicates the average of multiple experiments and can differ for individual sample materials. The measurement speed is determined by measuring the time between the movement of the robotic arm into the first measured well, and its movement out of the last measured well. The measurements were carried out at high flow rate in fast mode
Flow Rate25, 50, or 100 µL/min or automate flow rate to maintain 500, 1000, or 2000 events/second
Fluorescence5-decade logarithmic scale (10-2 to 103), displayed in lin, log, or hlog scales
Operating SystemEmbedded operating system
SoftwareMACSQuantify (integrated acquisition and analysis software)
ComponentsBuild-in PC, 15.6” LCD touchscreen, Robotic uptake unit for sample acquisition, integrated MACS magnetic cell enrichment unit.
AccessoriesMACS^® Chill Racks (5,15, 50, 96), Universal Reagent Rack (130-115-722), MACSQuant^® Buffer Supply Station (130-101-841), MACS^® MiniSampler Plus (130-105-745), MACSQuant^® Columns (130-094-458),

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Miltenyi Biotec

Miltenyi Biotec Inc.

2303 Lindbergh Street

Auburn, CA 95602

United States

Phone: Phone: +1 530 888 8871
Toll Free: 800 FOR MACS
Fax: +1 530 888 8925

Email: macs@miltenyibiotec.com

Company Profile

Website: www.miltenyibiotec.com

International Office

Miltenyi Biotec

Miltenyi Biotec B.V. & Co. KG

Friedrich-Ebert-Straße 68

51429 Bergisch Gladbach

Germany

Phone: +49 2204 8306-0
Fax: +49 2204 8306-235

Email: macs@miltenyibiotec.de

Company Profile

Website: www.miltenyibiotec.com

(1) EndoC-βH5 cells are storable and ready-to-use human pancreatic beta cells with physiological insulin secretionMolecular MetabolismOctober 1, 2023Bruno Blanchi, Marion Taurand, Claire Colace, Sofia Thomaidou, Charlotte Audeoud, Federica Fantuzzi, Toshiaki Sawatani, Sevda Gheibi, Joan Sabadell-Basallote, Fransje W.J. Boot, et al.(0.5 μl/105 cells, 563023 and 554680, BD Biosciences). Flow cytometry analyses were performed using MACSQuant 10 flow cytometer (Miltenyi). 2.5 Immunofluorescence EndoC-βH5 cells were seeded onto βCoat® coated 8-well

(2) SARS-CoV-2 specific plasma cells acquire long-lived phenotypes in human bone marrowEBioMedicineAugust 8, 2023Axel R. Schulz, Leonard Fiebig, Heike Hirseland, Lisa-Marie Diekmann, Simon Reinke, Sebastian Hardt, Antonia Niedobitek, Henrik E. Mei10 min. The cell pellet was resuspended in 10 mL PBE, and cells were counted volumetrically using a MACSQuant 10 (Miltenyi Biotech). Then, aliquots of 5 × 106 cells were prepared in 1 mL cryostorage tubes (LVL technologies),

(3) Chimeric antigen receptor (CAR) T cells for the treatment of a kidney transplant patient with post-transplant lymphoproliferative disorder (PTLD)Human Vaccines & ImmunotherapeuticsAugust 1, 2023Kathryn Kline, Wengen Chen, Michael E. Kallen, Rima Koka, Destiny Omili, Xiaoxuan Fan, Thierry Iraguha, Etse Gebru, Nishanthini Dishanthan, Jillian M. Baker, et al.were identified by 7-AAD dye exclusion (Miltenyi #130-111-568). Samples were acquired using a Miltenyi MACSQuant Analyzer 10 Flow Cytometer. CodePlex secretome analysis Cytokine concentrations in patient sera were quantified using the CodePlex

(4) SCCA1/SERPINB3 suppresses anti-tumor immunity and blunts therapy-induced T cell responses via STAT-dependent chemokine productionJournal of Clinical InvestigationAugust 1, 2023Liyun Chen, Victoria Shi, Songyan Wang, Lulu Sun, Rebecca N Freeman, Jasmine Yang, Matthew J. Inkman, Subhajit Ghosh, Fiona Ruiz, Kay Jayachandran, et al.True-Nuclear Transcription Factor Buffer Set, respectively (BioLegend). Stained cells were analyzed using a MACSQuant Analyzer 10 Flow Cytometer (Miltenyi Biotec). Antibody details are provided in Supplemental Table 3. Data analysis including viSNE

(5) Immune responses following neonatal vaccination with conserved F4 fragment of VtaA proteins from virulent Glaesserella parasuis adjuvanted with CAF®01 or CDAVaccine: XAugust 1, 2023Sergi López-Serrano, Yasser S. Mahmmod, Dennis Christensen, Thomas Ebensen, Carlos A. Guzmán, Fernando Rodríguez, Joaquim Segalés, Virginia Aragóndilution indicated in Table 1 in FACS buffer. After staining, cells were washed again and analyzed in a MACSQuant 10 Analyzer (Miltenyi Biotec, Bergisch Gladbach, Germany), acquiring twenty-thousand events per sample

(6) Truly tiny acoustic biomolecules for ultrasound imaging and therapybioRxivJuly 25, 2023Bill Ling, Bilge Gungoren, Yuxing Yao, Przemysław Dutka, Cameron A. B. Smith, Justin Lee, Margaret B. Swift, Mikhail G. Shapirowith 500 μL 0.25% trypsin-EDTA (Gibco), and diluted with 500 μL PBS prior to analysis with a Miltenyi MACSQuant Analyzer 10 flow cytometer. Bicone binding was quantified using the R1 channel. Data processing was performed in FlowJo. Gating

(7) X-ray and MR Contrast Bearing Nanoparticles Enhance the Therapeutic Response of Image-Guided Radiation Therapy for Oral CancerTechnology in Cancer Research & TreatmentJuly 20, 2023Gayatri Sharma, Mir Hadi Razeghi Kondelaji, Guru P. Sharma, Christopher Hansen, Abdul K. Parchur, Shayan Shafiee, Jaidip M. Jagtap, Brian Fish, Carmen Bergom, Eric Paulson, et al.PE Annexin V Apoptosis Detection Kit I, Cat# 559763). After staining, Annexin V + apoptotic cells were acquired on a MACSQuant 10 Analyzer Flow Cytometer (Miltenyi) and analyzed using FlowJo software version 10.0 (BD Life Sciences).

(8) Impact of Early Natural Killer Cell Reconstitution on the Outcomes of T Cell-Replete Allogeneic Hematopoietic Stem Cell TransplantationJournal of Inflammation ResearchJuly 19, 2023Ziwei Zhou, Xuan Liu, Xuejun Zhang, Shupeng Wen, Huan Hua, Zhenzhen Wang, Zheng Xu, Yu Lu, Fuxu WangT lymphocytes and B lymphocytes in these samples at various time points after transplantation was detected by MACSQUANT 10 flow cytometry (Miltenyi Biotec). Lymphocyte surface antigen markers included: NK cells: CD3-CD56+,

(9) Deciphering the Action of Neuraminidase in Glioblastoma ModelsInternational journal of molecular sciencesJuly 19, 2023Baeza-Kallee N, Bergès R, Hein V, Cabaret S, Garcia J, Gros A, Tabouret E, Tchoghandjian A, Colin C, Figarella-Branger D.that are required for the A2B5 antibody’s binding. 4.2. Stainings Flow cytometry was performed using MACSQuant® 10 (Miltenyi Biotec SAS, Paris, France) on dissociated cells using A2B5 (mouse APC-IgM, clone 105, Miltenyi

(10) Evaluation of the innate immune response of caprine neutrophils against Mycobacterium avium subspecies paratuberculosis in vitroVeterinary ResearchJuly 18, 2023Miguel Criado, Valentín Pérez, Noive Arteche-Villasol, Natalia Elguezabal, Elena Molina, Julio Benavides, Daniel Gutiérrez-Expósitowashed twice and resuspended in PBS with 1% BSA. Afterwards, flow cytometry data was acquired using MACSQuant® Analyzer 10 flow cytometer (Miltenyi Biotec, San Diego, CA, USA). Gating strategy is detailed in Figure 1. Analysis of cytokine

(11) Endothelial ILK induces cardioprotection by preventing coronary microvascular dysfunction and endothelial-to-mesenchymal transitionBasic Research in CardiologyJuly 14, 2023P. Reventun, S. Sánchez-Esteban, A. Cook-Calvete, M. Delgado-Marín, C. Roza, S. Jorquera-Ortega, I. Hernandez, L. Tesoro, L. Botana, J. L. Zamorano, et al.488 and Alexa Fluor 647. For flow cytometry, 106 cells were used. Data acquisition was performed on a MACSQuant 10 flow cytometer (Miltenyi Biotec) and the data were analysed with the MACSQuant software. After pre-selection

(12) Dimethyl fumarate-related immune and transcriptional signature is associated with clinical response in multiple sclerosis-treated patientsFrontiers in ImmunologyJuly 7, 2023Alicia Sánchez-Sanz, Santiago García-Martín, Julia Sabín-Muñoz, Irene Moreno-Torres, Víctor Elvira, Fátima Al-Shahrour, Aranzazu García-Grande, Elvira Ramil, Ofir Rodríguez-De la Fuente, Beatriz Brea-Álvarez, et al.panels. All FCAbs were purchased from Miltenyi Biotec. Data were acquired using a digital flow cytometer (MACSQuant 10, Miltenyi Biotec) and analyzed using MACSQuantify (Miltenyi Biotec) and FlowJo (BD Biosciences) software. 2.4

(13) Impaired DNA double-strand break repair and effective radiosensitization of HPV-negative HNSCC cell lines through combined inhibition of PARP and Wee1Clinical and translational radiation oncologyJuly 1, 2023Oetting A, Christiansen S, Gatzemeier F, Köcher S, Bußmann L, Böttcher A, Stölzel K, Hoffmann AS, Struve N, Kriegs M, et al.Cells were washed once with PBS/0.5% BSA/0.1% Triton X-100 before flow cytometric analysis using a MACSQuant10 with MACSQuantify Software (Miltenyi Biotec). The proportion of cells in the respective cell cycle phases

(14) Scale-Up of Academic Mesenchymal Stromal Cell ProductionJournal of Clinical MedicineJune 30, 2023Caroline Laroye, Mélanie Gauthier, Jessica Morello, Naceur Charif, Véronique Latger Cannard, Céline Bonnet, Alain Lozniewski, Andrei Tchirkov, Natalia De Isla, Véronique Decot, et al.Immunophenotype Analysis After each trypsinization step, an MSC count was performed by flow cytometry (MACSQuant10, Miltenyi Biotec, Bergisch Gladbach, Germany). The proliferation potential of WJ-MSCs during monolayer

(15) Association between the Immunophenotype of Peripheral Blood from mCRPC Patients and the Outcomes of Radium-223 TreatmentDiagnostics (Basel)June 29, 2023Elisabet Cantó, Georgia Anguera, Natalia Jiménez, Begoña Mellado, Ona Ramírez, Anais Mariscal, Pablo Maroto, Silvia Vidaltimes, and cells were resuspended in 200 µL of phosphate buffer solution. Samples were acquired with the MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotec) and the analyses (the percentage of positive cells (%) and count/µL) for each population

(16) The Fitness of Beta-Lactamase Mutants Depends Nonlinearly on Resistance Level at Sublethal Antibiotic ConcentrationsmBioJune 27, 2023Andrew D. Farr, Diego Pesce, Suman G. Das, Mark P. Zwart, J. Arjan G. M. de Visserwells of a 96-well plate, and BFP+ and YFP+ cellular events were quantified by flow cytometry using a Macsquant10 (Miltenyi Biotec). The competition plates were then sealed with Breathe-Easier sealing film (Diversified

(17) Impaired Regulation by IL-35 in Systemic SclerosisInternational Journal of Molecular SciencesJune 24, 2023Rubén Osuna-Gómez, Ivan Castellví, Maria Mulet, Mª Àngels Ortiz, Douglas E. Brough, Helen Sabzevari, Roshanak T. Semnani, Silvia VidalBiotech), and CD8-PE-Cy7 (Biolegend) antibodies. The data acquisition and analysis were performed on a MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotech) using FlowJo version 10. For the data analysis, doublets were excluded and single

(18) Method for estimation of apoptotic cell fraction of cytotherapy using in vivo fluorine-19 magnetic resonance: pilot study in a patient with head and neck carcinoma receiving tumor-infiltrating lymphocytes labeled with perfluorocarbon nanoemulsionJournal for ImmunoTherapy of CancerJune 19, 2023Eric T Ahrens, Brooke M Helfer, Charles F O’Hanlon, Deanne R Lister, Julie L Bykowski, Karen Messer, Benjamin I Leach, Jiawen Chen, Hongyan Xu, Gregory A Daniels, et al.then stained with anti-IFNγ-APC (Miltenyi Biotec). Cellular preparations were assayed using a Miltenyi MACSQuant 10 analyzer. 19F nuclear magnetic resonance assay of F-TIL labeling To assay the mean 19F/cell of the

(19) Novel Fas-TNFR chimeras that prevent Fas ligand-mediated kill and signal synergistically to enhance CAR T cell efficacyMolecular Therapy - Nucleic AcidsJune 13, 2023Callum McKenzie, Mohamed El-Kholy, Farhaan Parekh, Mathew Robson, Katarina Lamb, Christopher Allen, James Sillibourne, Shaun Cordoba, Simon Thomas, Martin Puledetected using an anti-CD34 antibody. Flow cytometry and antibodies Flow cytometry was performed using MACSQuant 10 and X flow cytometers (Miltenyi Biotec). All staining, unless specified otherwise, was performed at

(20) β3-adrenergic receptor on tumor-infiltrating lymphocytes sustains IFN-γ-dependent PD-L1 expression and impairs anti-tumor immunity in neuroblastomaCancer gene therapyJune 1, 2023Bruno G, Nastasi N, Subbiani A, Boaretto A, Ciullini Mannurita S, Mattei G, Nardini P, Della Bella C, Magi A, Pini A, et al.Antibody 12-5982-82, eBioscience™) for cytofluorimetric quantification. Stained cells were acquired on a MACSQuant Analyzer 10 Flow Cytometer (Miltenyi Biotec, Gladbach, Germany), and data were processed using Flowlogic software (Miltenyi Biotec,

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(1)

12/17/2018MACsQuant10 to Analyze My Data to the MAX Allison M.The MACsQuant10 definitely had a steep learning curve compared to other flow cytometry machines. (Miltenyi Biotech offers a 4-day course to learn the ins and outs which I really benefited from.) ... Read Review
(1)

6/26/2015MQ10 Flow Cytometer Margaret DoyleWe are examining the role of innate and adaptive immune cells in disease states in large population studies. We needed a machine capable of handling 96-well plates, 8-colors plus FSC and SSC, and ... Read Review

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MACSQuant^® Analyzer 10 Flow Cytometer from Miltenyi Biotec

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Miltenyi Biotec

Miltenyi Biotec

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MACSQuant^® Analyzer 10 Flow Cytometer from Miltenyi Biotec

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