Invitrogen™ Attune™ CytPix™ Flow Cytometer from Thermo Fisher Scientific

Product Specs
ItemInvitrogen™ Attune™ CytPix™ Flow Cytometer
CompanyThermo Fisher Scientific
Price Get Pricing Get Quote for this Product
Catalog NumberA51848
Detection2 scatter channels, up to 14 fluorescence channels; Brightfield imaging capability
QuantityEA
Sample Volume20 µL to 4 mL
Flow Rate12.5 to 1,000 µL/min
Plate FormatsAccommodates tubes from 17 x 100 mm to 8.5 x 45 mm
Sensitivity≤ 80 molecules of equivalent soluble fluorochrome (MESF) for FITC, ≤ 30 MESF for PE, ≤70 MESF for APC
ResolutionCV < 3% for the singlet peak of propidium iodide–stained chicken erythrocyte nuclei (CEN)
PixelsResolution - 0.3 microns/pixel
Objectives20x magnification
Imaging Size96 x 96 pixels – 248 x 248 pixels
Field of View (FOV)29 x 29 um – 74 x 74 µm
Dimensions(WxDxH) 58 x 43 x 49 cm (23 x 17 x 19 in.)
Lasers2 to 4 lasers
Excitation488 nm (50 mW), 405 nm (50 mW), 637 nm (100 mW), 561 nm (50 mW)
Parameters14 fluorescence channels
CapacityTubes (1 mL, 5 mL, 15 mL)
Data Throughput35,000 events/sec

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Website: www.thermofisher.com

(1) Respiration is essential for aerobic growth of Zymomonas mobilis ZM4mBioNovember 1, 2023Magdalena M. Felczak, Matthew P. Bernard, Michaela A. TerAvestjust prior to acquisition. CellROX Green (BL1-A) and Sytox Red (RL1-A) fluorescence was assessed on an Attune CytPix (ThermoFisher Scientific) flow cytometer in the MSU Flow Cytometry Core Facility. Post-acquisition analysis

(2) High-Dimensional Mass Cytometry Analysis of Embryonic Antigens and Their Signaling Pathways in Myeloid Cells from Bone Marrow Aspirates in AML Patients at DiagnosisCancers (Basel)September 25, 2023Carmen-Mariana Aanei, Estelle Devêvre, Adrian Șerban, Emmanuelle Tavernier-Tardy, Denis Guyotat, Lydia Campos CatafalPharmingen, San Diego, CA, USA) for 20 min at room temperature in the dark. Data were acquired using an Attune CytPix instrument (Thermo Fisher Scientific). The compensation settings were obtained with CompBeads (BD Biosciences). 2.6.

(3) Myeloblasts transition to megakaryoblastic immunophenotypes over time in some patients with myelodysplastic syndromesPLOS ONESeptember 20, 2023Kiyoyuki Ogata, Yuto Mochimaru, Kazuma Sei, Naoya Kawahara, Mika Ogata, Yumi Yamamotoblasts and other cells. We excluded these three cases from the present data. Imaging flow cytometry An Attune CytPix Flow Cytometer (Thermo Fisher Scientific Co., Fair Lawn, NJ, USA) was used to analyze the selected samples.

(4) 8-Methoxypsoralen Induces Apoptosis by Upregulating p53 and Inhibits Metastasis by Downregulating MMP-2 and MMP-9 in Human Gastric Cancer CellsBiomolecules & TherapeuticsMarch 1, 2023Eun Kyoung Choi, Hae Dong Kim, Eun Jung Park, Seuk Young Song, Tien Thuy Phan, Miyoung Nam, Minjung Kim, Dong-Uk Kim, Kwang-Lae Hoeroom temperature, according to the manufacturer’s instructions. Flow cytometry was performed using Attune CytPix (Thermo-Fisher Scientific). Ten thousand events per sample were counted in duplicate. Western blot analysis

(5) Detection of Circulating Tumor Cells Using the Attune NxTInternational journal of molecular sciencesDecember 20, 2022Gruijs M, Zeelen C, Hellingman T, Smit J, Borm FJ, Kazemier G, Dickhoff C, Bahce I, de Langen J, Smit EF, et al.presented a relatively high amount of EpCAM+EGFR+ events, these cells were studied in more detail using the Attune CytPix (Thermo Fisher Scientific, Waltham, MA, USA). The Attune CytPix is an extension of the Attune NxT with

(6) Cefazolin/BMP-2-Loaded Mesoporous Silica Nanoparticles for the Repair of Open Fractures with Bone DefectsOxidative Medicine and Cellular LongevitySeptember 20, 2022Mingkui Shen, Lulu Wang, Li Feng, Chuangye Xu, Yi Gao, Sijing Li, Yulan Wu, Guoxian Peito the above mixture solution. After mixing and incubating in the dark for 15 min, flow cytometry (Attune CytPix, Thermo Fisher Scientific) was used to detect lymphocyte apoptosis. 2.9. Therapeutic Effect Detection

(7) Immunophenotyping: Analytical approaches and role in preclinical development of nanomedicinesAdvanced Drug Delivery ReviewsJune 1, 2022Hannah S. Newton, Marina A. DobrovolskaiaImage Cytometry,Celigo Image Cytometer,Nexcelom Bioscience,Viability of tumor spheroids [98] Image Cytometry,Attune™ CytPix™ Flow Cytometer,Invitrogen,"Immunophenotyping, cell apoptosis [99]" Mass Cytometry,Helios™ mass cytometer,Fluidigm,"Immunophenotyping

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