Cytek Northern Lights™ Flow Cytometer from Cytek Biosciences

Product Specs
ItemCytek Northern Lights^™ Flow Cytometer
CompanyCytek Biosciences
Price Get Pricing Get Quote for this Product
Catalog NumberNorthern Lights
Dimensions(WxDxH) 54 x 52 x 52 cm - without loader
(WxDxH) 58.4 x 62 x 52 cm - with loader
QuantityEA
ApplicationsCancer Research, Immuno-oncology, Immunotherapy, Immunophenotyping, Immune Monitoring, Hematology, Cell Therapies, Stem Cell Research, Gene Editing Assays, Quality Control, Cell Cycle Analysis, Apoptosis, Bead Based Assays
Detection3 scatter detectors (FSC, SSC-B, SSC-V). And up to 38 high-resolution fluorescent detectors using Avalanche Photo Diodes (APDs)
Lasers1 to 3 lasers
Parameters38 fluorescence channels
CapacityTubes (5 mL), Microplates (96-well)
Data Throughput35,000 events/sec
Flow Rate15 to 100 µL/min
ThroughputHigh-throughput mode capable of running a 96-well plate in 27 minutes. Low carryover mode with ≤ 0.1% carryover.
ResolutionCapable of resolving 70 nm beads from noise using SSC-V with the Enhanced Small Particle (ESP) Detection Option.
Excitation405 nm (100 mW), 488 nm (50 mW), 640 nm (80 mW)

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Cytek Biosciences

47215 Lakeview Blvd.

Fremont, CA 94538

United States

Phone: (877) 922-9835

Email: products@cytekbio.com

Company Profile

Website: https://cytekbio.com/

(1) A bile acid-GPBAR1 network supports anti-inflammatory and anti-fibrotic benefits of probiotics in colitisGut MicrobesDecember 31, 2026Michele Biagioli, Cristina Di Giorgio, Silvia Marchianò, Benedetta Sensini, Ginevra Urbani, Eleonora Giannelli, Ginevra Lachi, Carmen Massa, Maria Rosaria Sette, Francesca Paniconi, et al.fraction. Flow-cytometry Cells isolated from the colonic lamina propria were examined via spectral flow cytometry using a Cytek® Northern Lights™ cytometer equipped with violet (405 nm), blue (488 nm), and red (640 nm) excitation lasers, facilitating

(2) Efficacy and immunogenic effects of Tumor Treating Fields ( TTFields ) in preclinical models of pancreatic ductal adenocarcinoma, with and without gemcitabine/nab‐paclitaxelInternational Journal of CancerJune 15, 2026Tal Kan, Tharwat Haj Khalil, Yiftah Barsheshet, Tali Voloshin, Lilach Koren, Bella Koltun, Cfir David, Kerem Wainer‐Katsir, Avital Vorontsova, Boris Brant, et al.and expressed as fold change relative to control. 2.6 Cell count Cells were counted using Cytek® Northern Lights (Cytek® Biosciences, Fremont, CA) flow cytometer, and cell count presented as the percentage relative

(3) Loss of cystathionine-β-synthase contributes to elevated OXPHOS, a vulnerability in Ara-C-resistant Myeloid Leukemia in Down syndromeBiochemical PharmacologyMay 1, 2026Jenna Thibodeau, Jianlei Zhao, Holly Edwards, Lisa Polin, Juiwanna Kushner, Sijana H Dzinic, Kathryn White, Kian Hershberger, Yongwei Su, Tasnim Arroum, et al.isothiocyanate (FITC, 640945, BioLegend, San Diego, CA, USA). Flow cytometry was performed using the Cytek Northern Lights at the Microscopy, Imaging, and Cytometry Resources Core at KCI and data is presented as previously

(4) Preclinical evaluation of an mRNA vaccine developed from the first human isolate of bovine H5N1Cell Rep MedApril 21, 2026Hongli Li, Mengting Huang, Simin Feng, Wansheng Li, Dandan Wei, Xuechun Li, Yun Quan, Hao Gu, Ting Jiang, Yan Liu, et al.IFN-γ, IL-5, IL-2, TNF-α, GM-CSF, IL-4, IL-13, and IL-6. The processed samples were analyzed on a CYTEK Northern Lights full spectrum cell analyzer (NL-CLC V16B14R8). After the recovery period of these toxicity tests, animals

(5) Single-cell analysis highlights the significance of malignant cell IFN/MHC-II for immunotherapy response in head and neck squamous cell carcinomaCell Reports MedicineApril 21, 2026Michael Mints, Reilly A. Sample, Anuraag S. Parikh, Jesse M. Zaretsky, Zongtai Qi, Travis Law, Fudong Wang, Thomas F. Barrett, Riley Mullins, Ashley Reeb, et al.mouse EpCAM (G8.8) was obtained from Thermo Fisher. Flow cytometry data were acquired using the Cytek Northern Lights (Cytek) full-spectrum profiling system. Optimal antibody combinations were determined using Cytek Cloud

(6) A Plasma-Functionalized ECM Platform for Intraoral Inflammation Control: Comparative Effects of Hyaluronic Acid and N-Acetyl-L-Cysteine on Oral Keratinocyte ResponsePolymers (Basel)April 17, 2026Pedro U. Muñoz-González, Pascale Chevallier, Leyla Desparois, Sylvie Louise Avon, Fatiha Chandad, Diego Mantovani, Vanessa P. Houdemanufacturer’s protocol. Mitochondrial superoxide levels were then assessed by flow cytometry on a Cytek Northern Lights (Cytek Biosciences, Fremont, CA, USA), and analyzed with FlowJo (BD Biosciences, Mississauga, ON, CA). 2.3.

(7) Lymphocyte Antigen 6G Mediates Vagotomy-Associated Reduction in Body WeightFASEB JApril 15, 2026Ting Liu, April S. Caravaca, Min Cai, Wanmin Dai, Jérômine Jeanne Vacquié, Qi Guo, Peter Holicek, Maxime Villet, Patricia Teixeira Mendes, Yuyang Zhang, et al.#00–5521‐00) following staining with extracellular antibodies. Flow cytometry was performed using Cytek Northern Lights (Cytek, #NL‐3000). 2.10 Immunofluorescence eWAT was collected from male mice and fixed using 4% formaldehyde

(8) Subunit vaccination using Atomic Layering Thermostable Antigen and Adjuvant (ALTA®) platform elicits enhanced humoral and cellular immune responsesFront ImmunolApril 15, 2026Daria L. Ivanova, Matthew S. Lewis, Annie B. Caplan, Isabella R. Walters, Emma M. Snyder, Keith A. Strand, Lorena R. Antunez, Sineenart Sengyee, Sarah B. Weiby, Federico Urbano-Munoz, et al.receptors or Myeloid CD64, respectively. All samples were analyzed via flow cytometry using a Cytek Northern Lights 3-laser (VBR) spectral flow cytometer and the FlowJo software (BD Biosciences). 2.6 ELISpot Spleens

(9) Thalidomide-mediated immunomodulation in a patient with IL-10RA deficiency-associated very early-onset inflammatory bowel diseaseFront ImmunolApril 15, 2026Aiyan Ren, Qian Zeng, Bin Zhang, Xue Chuan, Yalin Wu, Meiting Wang, Huan Ren, Yan Chen, Pei Huang, Zuochen Dustained according to the manufacturer’s instructions. All samples were analyzed on a Cytek® Aurora/Northern Lights™ (Cytek, DE, USA), and data were analyzed using FlowJo analysis software. Western blotting The PBMCs

(10) Guanylate-binding proteins balance iNOS/Arg-1 in myeloid cells during L. major infection and promote host defense to infectionmBioApril 8, 2026Lucy Fry, Het Adhvaryu, Hayden Roys, Anne Bowlin, Gopinath Venugopal, Jordan T. Bird, Charity Washam, Masahiro Yamamoto, Jörn Coers, Stephanie Byrum, et al.antibodies against Arg-1 PE (Invitrogen) or iNOS APC (Biolegend). Cell events were acquired on a Cytek Northern Lights (Cytek) and analyzed using FlowJo (Tree Star). Statistics All data were analyzed for statistical significance

(11) Deletion 9p drives B-ALL through the heterozygous inactivation of Pax5 and Cd72 in pre-leukemic cellsJCI InsightApril 8, 2026Belén Ruiz-Corzo, Ana Casado-García, Ninad Oak, Paula Somoza-Cotillas, Andrea López-Álvarez de Neyra, Jorge Martínez-Cano, Alba Pérez-Pons, Elena G. Sánchez, Oscar Blanco, Diego Alonso-López, et al.μg/mL PI to exclude dead cells from further analysis. The samples and the data were acquired in a Cytek Northern Lights 2000 spectral cytometer (2 lasers, red and blue) and analyzed with FCS Express software. Specific fluorescence

(12) Cryoablation Plus Immune Checkpoint Inhibitors Enhanced Dendritic Cell and T Cell Activation in TNBC Murine ModelImmunotargets TherApril 7, 2026Flavia Sardela de Miranda, Rachel L Babcock, Maria F Mahecha, Prrishti J Gukhool, Geetha P Boligala, Amanda K Garcia, Dalia Martinez-Marin, Ava G Oliver, Thomas Hintelmann, Kathryn L Furr, et al.CD16/CD32 antibody mixture (Invitrogen, San Diego, CA). Flow cytometry data was acquired on a Cytek Northern Lights™-Clinical (NL-CLC) spectral analyzer (Cytek Biosciences, Freemont, CA) using SpectroFlo® software and

(13) CRISPR Screens Reveal Epstein-Barr Virus-activated JunB as a Key Lymphoblastoid B cell Dependency Factor that Represses Cyclin Dependent Kinase Inhibitor P18INK4cbioRxivApril 1, 2026Eric M. Burton, Yifei Liao, Davide Maestri, Bidisha Mitra, Benjamin E. Gewurzcells/mL. Labeled cells were analyzed by flow cytometry using a BD FACSCalibur instrument or Cytek Northern Lights instrument and analysis was performed with FlowJo V10. PI cell cycle analysis Cells were collected

(14) Sulfur partitioning from cysteine controls T cell proliferation and effector functionCellMarch 31, 2026Beth Kelly, Minsun Cha, Tatjana Gremelspacher, Jacob L. Martin, Massimo Andreis, Isha Maloo, Gustavo E. Carrizo, Mia Gidley, Michal A. Stanczak, Petya Apostolova, et al.anti-CD3/28 + IL-2. Flow cytometry analyses were performed on the FACSymphony (BD Biosciences) or Cytek Northern Lights, and then analyzed with FlowJo 10 Software (TreeStar). CellROX, MitoTracker Green (50 nM), labile iron

(15) Early enhanced control of Plasmodium yoelii infection in IL-10–deficient mice is independent of IFN-γ, IL-12, and the humoral responsebioRxivMarch 31, 2026Meghan D. Jones, Kara A. O’Neal, Sheldon L. Zeltner, Alison R. Gouch, Jason S. Stumhoferminutes at room temperature. Following resuspension in FACS buffer, samples were acquired on a Cytek Northern Lights (Cytek Biosciences) spectral flow cytometer. All data were analyzed using FlowJo version 10 software.

(16) HIV-1 infection does not confer intrinsic resistance to cell death induced by cytotoxic T lymphocytesbioRxivMarch 27, 2026Niklas Bachmann, Bailey Kim, Colin Kovacs, Steven Deeks, Rebecca Hoh, Francesco R. Simonetti, Janet D. Siliciano, Robert F. Silicianopreviously described (70). Flow cytometry. All flow cytometry experiments were performed on a Cytek Northern Lights spectral flow cytometer (3L V-B-R configuration) using the SpectroFlo software (version 3.3.0). Single-color

(17) Pharmacologic targeting of the dopamine D2 receptor impacts the efficacy of immune checkpoint blockade in melanomaJ Immunother CancerMarch 27, 2026Mark Gregory, Justin B Hackett, Savannah Hughes, Maria C Muñiz, Samantha Ayoub, Alexander Boerner, Habib Alrabaa, Lucien Taylor, Brianna Rivera, Hanna Holland, et al.surface staining, cells were washed twice with 1× PBS containing 1% FBS. All data were acquired on Cytek NorthernLights 3L V/B/R (Cytek) and analyzed using FCS Express 7 (De Novo Software). Quantitative reverse transcription

(18) Aminosteroid RM-581 Induces G0/G1 Arrest and Endoplasmic Reticulum Stress-Mediated Apoptosis in Human Acute and Chronic Leukemia Cell LinesCancers (Basel)March 26, 2026Maude Fleury, Jenny Roy, René Maltais, Francine Durocher, Donald Poirier350× g and resuspended in 300 µL of PBS. Finally, the cells were analyzed by flow cytometry using a Northern Lights flow cytometer (Cytek Biosciences Inc., Fremont, CA, USA) on the Flow Cytometry Platform (CHU de Québec-Université

(19) Effects of Human Recombinant Growth Hormone (rhGH) Treatment on Plasma Extracellular Vesicles in GH-Deficient Children: A Preliminary ReportJournal of Clinical MedicineMarch 26, 2026Antonello E. Rigamonti, Luca Ferrari, Chiara Favero, Mirjam Hoxha, Adele Bondesan, Nicoletta Marazzi, Silvano G. Cella, Alessandro Sartoriomeasurements of the EVs (count/mL in plasma). The cell origin of EVs was characterized by flow cytometry using Northern lights (Cytek) spectral cell analyzer. Shortly, Kisker Biotech silica particles plain fluo green (100, 200,

(20) A probiotic bacterium modulates antitumour γδ T-cell responses in lung cancerFront ImmunolMarch 24, 2026Yoshihiko Goto, Garry Dolton, Hannah Thomas, Théo Morin, Yuka Tajima, Kosuke Imamura, Shinya Sakata, Kentaro Oka, Atsushi Hayashi, Motomichi Takahashi, et al.expression were analyzed by flow cytometry using a FACS Canto II (BD Biosciences, New Jersey, USA) or Cytek Northern Lights (Cytek Japan). The data obtained by flow cytometry were analyzed with FACS Diva v9.0 (BD) and FlowJo

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Cytek Northern Lights^™ Flow Cytometer

Cytek Northern Lights^™ Flow Cytometer from Cytek Biosciences

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Cytek Biosciences

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Cytek Northern Lights^™ Flow Cytometer from Cytek Biosciences

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