FluoroFix™ Buffer from BioLegend

Product Specs
ItemFluoroFix™ Buffer
CompanyBioLegend
Price $153.00Supplier Page View Company Product Page
Catalog Number422101
ApplicationsFC, ICFC - Quality tested, ICC - Reported in the literature, not verified in house
Quantity200 tests

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(41) Enhancer Clusters Drive Type I Interferon-Induced TRAIL Overexpression in Cancer, and Its Intracellular Protein Accumulation Fails to Induce ApoptosisCancersFebruary 3, 2023Di Benedetto C, Khan T, Serrano-Saenz S, Rodriguez-Lemus A, Klomsiri C, Beutel TM, Thach A, Walczak H, Betancur P.with LIVE/DEAD Fixable Violet Dead as above, fixed in FluoroFix buffer (Biolegend; catalogue number 422101) for 1 h at room temperature and permeabilized using Intracellular Staining Permeabilization Wash Buffer

(42) Highly efficacious and safe neutralizing DNA aptamer of SARS-CoV-2 as an emerging therapy for COVID-19 diseaseVirology journalDecember 30, 2022Ayass MA, Tripathi T, Griko N, Pashkov V, Dai J, Zhang J, Herbert FC, Ramankutty Nair R, Okyay T, Zhu K, et al.IC108A), and Fixable Viability Dye eFluor 450 (Invitrogen # 65-0863-14) were used. FluoroFix Buffer (# 422101) was purchased from BioLegend. COVID-19 S Protein/(GFP)-(6His) VLP (#VLP001) was purchased from Gentarget,

(43) Discovery of Cyclic Peptide Inhibitors Targeting PD-L1 for Cancer ImmunotherapyJournal of medicinal chemistrySeptember 22, 2022Fetse J, Zhao Z, Liu H, Mamani UF, Mustafa B, Adhikary P, Ibrahim M, Liu Y, Patel P, Nakhjiri M, et al.°C. For intracellular staining, the surfaced-stained cells were fixed with FluoroFix™ Buffer (Cat# 422101, Biolegend, San Diego, CA) for 20 min at room temperature. The fixed cells were permeabilized with a

(44) Radioresistant cells initiate lymphocyte-dependent lung inflammation and IFNγ-dependent mortality in STING gain-of-function miceProceedings of the National Academy of Sciences of the United States of AmericaJune 21, 2022Gao KM, Motwani M, Tedder T, Marshak-Rothstein A, Fitzgerald KA.antibodies as documented in SI Appendix, Table S1. Samples were fixed using Fluorofix buffer (Biolegend #422101). Intracellular staining was performed using a BD Biosciences Fixation/Permeabilization kit (BD Biosciences

(45) Flow cytometry analysis of the subpopulations of mouse keratinocytes and skin immune cellsSTAR protocolsMarch 18, 2022Sakamoto K, Goel S, Funakoshi A, Honda T, Nagao K.assays ,, Zombie Aqua Fixable Viability Kit,BioLegend,Cat# 423101 FluoroFix™ Buffer,BioLegend,Cat# 422101 eBioscience™ Foxp3/Transcription Factor Staining Buffer Set,Thermo Fisher Scientific,Cat# 00-5523-00 ,, Experimental models

(46) Lactobacillus johnsonii alleviates colitis by TLR1/2-STAT3 mediated CD206(+) macrophages(IL-10) activationGut microbesJanuary 1, 2022Jia DJ, Wang QW, Hu YY, He JM, Ge QW, Qi YD, Chen LY, Zhang Y, Fan LN, Lin YF, et al.for 30 minutes in the dark. Then, cells were fixed in the dark at room temperature for 30 minutes (422101, Biolegend), centrifuged, and then the membranes were ruptured (421002, Biolegend). Intracellular staining

(47) NK and CD8+ T cell phenotypes predict onset and control of CMV viremia after kidney transplantJCI insightNovember 8, 2021Pickering H, Sen S, Arakawa-Hoyt J, Ishiyama K, Sun Y, Parmar R, Ahn RS, Sunga G, Llamas M, Hoffmann A, et al.25 minutes. After washed with FACS buffer, samples were fixed in 100 μl Fluorofix Buffer (BioLegend, 422101). Samples were analyzed on a LSRFortessa X50 (FACSymphony) cytometer (BD Bioscience). Data were analyzed

(48) Disruption of the endopeptidase ADAM10-Notch signaling axis leads to skin dysbiosis and innate lymphoid cell-mediated hair follicle destructionImmunityOctober 12, 2021Sakamoto K, Jin SP, Goel S, Jo JH, Voisin B, Kim D, Nadella V, Liang H, Kobayashi T, Huang X, et al.Pharmingen,Cat# 550583 Zombie Aqua Fixable Viability Kit,Biolegend,Cat# 423101 FluoroFix™ Buffer,Biolegend,Cat# 422101 eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set,Thermo Fisher Scientific,Cat# 00-5523-00 Opti-MEM™ I Reduced Serum Medium

(49) Optimization of antigen-specific CD8(+) T cell activation conditions for infectious diseases including COVID-19STAR protocolsSeptember 17, 2021Xiao C, Qiu C, Deng J, Ye J, Gao L, Su J, Luo OJ, Wang P, Chen G.25°CTip: For mild fixation (especially with tandem fluorophore), FluoroFix™ Buffer (BioLegend Cat# 422101) can be used.a.Centrifuge at 300 × g for 5 min at 4°C, and discard supernatantPause point: To hold the

(50) Small RNAs are modified with N-glycans and displayed on the surface of living cellsCellJune 10, 2021Ryan A. Flynn et al.specific",Jackson ImmunoResearch,Cat# 209–605-098 J2 antibody,Scicons,Cat# J2 FluoroFix Buffer,BioLegend,Cat# 422101 TrypLE,Thermo Fisher Scientific,Cat# 12604021 Trypsin LC-MS grade,Promega,Cat# V5117 Trypsin,HyClone,Cat#

(51) Intratumoral CD4⁺ T Cells Mediate Anti-tumor Cytotoxicity in Human Bladder CancerCellJune 25, 2020Oh DY, Kwek SS, Raju SS, Li T, McCarthy E, Chow E, Aran D, Ilano A, Pai CS, Rancan C, et al.temperature and washed twice with FACS wash. Cells were fixed with FluoroFix buffer (Biolegend, Cat# 422101) and washed once with FACS buffer. Cells were acquired the next day on a FACSymphony (Becton Dickinson)

(52) Tissue Determinants of Human NK Cell Development, Function, and ResidenceCellFebruary 20, 2020Dogra P, Rancan C, Ma W, Toth M, Senda T, Carpenter DJ, Kubota M, Matsumoto R, Thapa P, Szabo PA, et al.for 25 min on ice, washed with FACS-buffer, and fixed in 100 μL of Fluorofix Buffer (BioLegend, cat# 422101). Samples were analyzed on the LSR Fortessa X50 (‘‘Symphony’’) cytometer (BD Bioscience). Data were

(53) Arming oHSV with ULBP3 drives abscopal immunity in lymphocyte-depleted glioblastomaJCI insightJuly 11, 2019Wirsching HG, Zhang H, Szulzewsky F, Arora S, Grandi P, Cimino PJ, Amankulor N, Campbell JS, McFerrin L, Pattwell SS, et al.(R&D Systems; FAB110A). Finally, cells were washed and resuspended in FluorFix fixation buffer (BioLegend, 422101). Samples were run on a BD Biosciences LSR Fortessa; data were acquired using FACSDiva software (BD

(54) Comprehensive analysis of mouse retinal mononuclear phagocytesNature ProtocolsJune 1, 2017Anika Lückoff, Rebecca Scholz, Florian Sennlaub, Heping Xu, Thomas Langmannusing a 450/50 band pass filter (equivalent to Pacific Blue®)  FluoroFix Buffer (Biolegend, cat. no. 422101, storage at at 2-8 °C) ! CAUTION The fixation buffer contains paraformaldehyde, which is toxic and mutagenic.  1.5ml

(55) Multidimensional pooled shRNA screens in human THP-1 cells identify candidate modulators of macrophage polarizationPloS oneJanuary 1, 2017Surdziel E, Clay I, Nigsch F, Thiemeyer A, Allard C, Hoffman G, Reece-Hoyes JS, Phadke T, Gambert R, Keller CG, et al.

(56) Impaired P2X1 Receptor-Mediated Adhesion in Eosinophils from Asthmatic PatientsJournal of immunology (Baltimore, Md. : 1950)June 15, 2016Wright A, Mahaut-Smith M, Symon F, Sylvius N, Ran S, Bafadhel M, Muessel M, Bradding P, Wardlaw A, Vial C.

(57) Preexisting Levels of CD4 T Cells Expressing PD-1 Are Related to Overall Survival in Prostate Cancer Patients Treated with IpilimumabCancer immunology researchSeptember 1, 2015Kwek SS, Lewis J, Zhang L, Weinberg V, Greaney SK, Harzstark AL, Lin AM, Ryan CJ, Small EJ, Fong L.

(58) Tasquinimod modulates suppressive myeloid cells and enhances cancer immunotherapies in murine modelsCancer immunology researchFebruary 1, 2015Shen L, Sundstedt A, Ciesielski M, Miles KM, Celander M, Adelaiye R, Orillion A, Ciamporcero E, Ramakrishnan S, Ellis L, et al.

(59) RNA sequencing of microglia and monocyte-derived macrophages from mice with experimental autoimmune encephalomyelitis illustrates a changing phenotype with disease courseJournal of neuroimmunologyDecember 15, 2014Lewis ND, Hill JD, Juchem KW, Stefanopoulos DE, Modis LK.

(60) [On the toxicology of carbromal. II. Pharmacokinetics of carbromal and its hypnotically active metabolites in the rat (author's transl)]Archives of toxicologyAugust 9, 1977H W Vohland, S Hadisoemarto, B Wanke

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FluoroFix™ Buffer from BioLegend

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FluoroFix™ Buffer from BioLegend

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