FluoroFix™ Buffer from BioLegend

Product Specs
ItemFluoroFix™ Buffer
CompanyBioLegend
Price $153.00Supplier Page View Company Product Page
Catalog Number422101
ApplicationsFC, ICFC - Quality tested, ICC - Reported in the literature, not verified in house
Quantity200 tests

Add to Compare List

BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(21) Sez6L2 autoimmunity induces cerebellar ataxia in micebioRxivMay 31, 2025Carlos J. Reyes-Sepúlveda, Julia Granato, John Randolph, Allison T. Ryan, Alison Hobbins, Angela Stout, Herman Li, Jennetta W. HammondCells were then washed three times with FACS buffer and incubated in FluoroFix™ buffer (BioLegend, 422101) for 60 minutes. The cells were resuspended in FACS buffer and incubated at 4°C overnight. The following

(22) Palbociclib and dsRNA sensor co-operate to enhance anti-cancer effects through ER stress and modulation of immune evasionNat CommunMay 25, 2025Victoria Roulstone, Joan Kyula-Currie, James Wright, Emmanuel C. Patin, Isaac Dean, Lu Yu, Aida Barreiro-Alonso, Miriam Melake, Jyoti Choudhary, Richard Elliott, et al.(65-0865-14, eBioscience) for 30 mins. Cells were washed in PBS + 5% FBS and fixed in FluoroFix buffer (422101, BioLegend) prior to analysis on the BD LSR II analyser or the BD Symphony A5. Data was collected using

(23) DNA methylation predicts infection risk in kidney transplant recipientsLife Sci AllianceMay 5, 2025Fei-Man Hsu, Harry Pickering, Liudmilla Rubbi, Michael Thompson, Elaine F Reed, Matteo Pellegrini, Joanna M Schaenmanand stained with fluorochrome-conjugated monoclonal antibodies, then fixed in FluoroFix buffer (Cat# 422101; BioLegend) using standard procedures as previously described (Schaenman et al, 2022). Antibodies used

(24) Polychlorinated biphenyls induce immunometabolic switch of anti-inflammatory macrophages toward an inflammatory phenotypePNAS NexusMarch 27, 2025Riley M Behan-Bush, Michael V Schrodt, Elizabeth Kilburg, Jesse N Liszewski, Laura M Bitterlich, Karen English, Aloysius J Klingelhutz, James A Ankrumtemperature, the macrophages were washed with PBS and fixed using FluoroFix Buffer (BioLegend, cat#: 422101). After 30 min of fixation, the cells were permeabilized using Intracellular Staining Permeabilization

(25) Identification of a conserved subset of cold tumors responsive to immune checkpoint blockadeJ Immunother CancerMarch 6, 2025Jade Moore, Jim Gkantalis, Ines Guix, William Chou, Kobe Yuen, Ann A Lazar, Matthew Spitzer, Alexis Combes, Mary Helen Barcellos-HoffThe supernatant was aspirated, and the cell pellet was in 0.5 mL/tube FluoroFix buffer (BioLegend Cat#422101) in the dark for 1 hour at room temperature for fixation. For intracellular staining, the nucleus was

(26) Bioactivities and Anti-Cancer Activities of NKT-Stimulatory Phenyl-Glycolipid Formulated with a PEGylated Lipid NanocarrierDrug Design, Development and TherapyNovember 20, 2024Jung-Tung Hung, Shih-Pin Chiou, Yun-Hsin Tang, Jing-Rong Huang, Fei-Yun Lo, Alice L Yuminutes at 4°C. For intracellular staining, splenocytes were fixed with 450 μL of FluroroFix buffer (#422101 BioLegend, San Diego, CA, US) for 15 minutes at room temperature. After fixation, the splenocytes were

(27) Bioactivities and Anti-Cancer Activities of NKT-Stimulatory Phenyl-Glycolipid Formulated with a PEGylated Lipid NanocarrierDrug Des Devel TherNovember 20, 2024Jung-Tung Hung, Shih-Pin Chiou, Yun-Hsin Tang, Jing-Rong Huang, Fei-Yun Lo, Alice L Yuminutes at 4°C. For intracellular staining, splenocytes were fixed with 450 μL of FluroroFix buffer (#422101 BioLegend, San Diego, CA, US) for 15 minutes at room temperature. After fixation, the splenocytes were

(28) Glucosylceramide synthase modulation ameliorates murine renal pathologies and promotes macrophage effector function in vitroCommunications biologyAugust 2, 2024Cheong A, Craciun F, Husson H, Gans J, Escobedo J, Chang YC, Guo L, Goncalves M, Kaplan N, Smith LA, et al.Next, the samples were centrifuged at 2000 rpm for 5 minutes and fixed in FluroFix buffer (Biolegend, 422101) for 20 minutes. Following that, the samples were analyzed using the Becton Dickinson LSRII flow cytometer. Primary

(29) Vimentin regulates mitochondrial ROS production and inflammatory responses of neutrophilsFrontiers in ImmunologyJuly 30, 2024Thao Ngoc Huynh, Jody Toperzer, Allison Scherer, Anne Gumina, Tonya Brunetti, Michael K. Mansour, David M. Markovitz, Brian C. Russo101207) for 30 minutes on ice. Samples were washed with FACS buffer, and fixed with FluoroFix (Biolegend, 422101) for 20 minutes on ice. Samples were resuspended in FACS buffer and analyzed using a BD LSRFortessa

(30) Activation of autoreactive lymphocytes in the lung by radioresistant cells expressing a STING gain-of-function mutationJCI InsightJuly 18, 2024Kevin MingJie Gao, Kristy Chiang, Sharon Subramanian, Xihui Yin, Paul J. Utz, Kerstin Nündel, Kate A. Fitzgerald, Ann Marshak-Rothsteinantibodies as documented in Supplemental Tables 3–6. Samples were fixed using Fluorofix buffer (BioLegend, 422101). Intracellular staining was performed using a BD Biosciences Fixation/Permeabilization kit (BD Biosciences,

(31) Fasting-mimicking diet remodels gut microbiota and suppresses colorectal cancer progressionNPJ biofilms and microbiomesJune 25, 2024Luo M, Wang Q, Sun Y, Jiang Y, Wang Q, Gu Y, Hu Z, Chen Q, Xu J, Chen S, et al.FITC (BioLegend Cat# 121419, RRID:AB_10709438). Cells were fixed with fixation buffer (BioLegend, Cat# 422101) in dark for 30 min at 4 °C. Cell samples were transferred into tubes and analyzed by Flow Cytometer

(32) Extracellular CIRP promotes Kupffer cell inflammatory polarization in sepsisFrontiers in ImmunologyMay 30, 2024Junji Shimizu, Atsushi Murao, Yongchan Lee, Monowar Aziz, Ping WangBV421 anti-mouse CD206 Ab (clone: C068C2; BioLegend) and then fixed in Fluorofix buffer (Cat. No.: 422101; BioLegend), followed by the intracellular staining with PE anti-mouse iNOS Ab (Cat. No.: sc-7271; Santa Cruz Biotechnology

(33) Oral bioavailability of a noncoding RNA drug, TY1, that acts on macrophagesbioRxivMay 4, 2024Shukuro Yamaguchi, Kazutaka Miyamoto, Xaviar M. Jones, Alessandra Ciullo, Ashley Morris, Kara Tsi, Eduardo Marbán, Ahmed G.E. Ibrahimcentrifugation at 800 × g for 5 minutes. Finally, cells were resuspended in 500 μl of FluoroFix Buffer (422101, BioLegend). Unstained and isotype control samples were also prepared. Statistical analysis Statistical

(34) Lack of TGFβ signaling competency predicts conversion of immune poor cancer to immune rich and response to checkpoint blockadebioRxivMarch 8, 2024Jade Moore, Jim Gkantalis, Ines Guix, William Chou, Kobe Yuen, Ann A. Lazar, Mathew Spitzer, Alexis J. Combes, Mary Helen Barcellos-Hoff4°C. Aspirated supernatant and resuspended cell pellet in 0.5ml/tube FluorFix buffer (Biolegend Cat#422101) in the dark for 1h at room temperature for fixation. For intracellular staining, the nucleus washed

(35) Immune cell phenotype and function patterns across the life course in individuals from rural UgandaFrontiers in immunologyJanuary 1, 2024Nalwoga A, Nakibuule M, Roshan R, Kwizera Mbonye M, Miley W, Whitby D, Newton R, Rochford R, Cose S.respectively, for 30 minutes at 4°C. Stained PBMCs were fixed using the FluoroFix buffer (BioLegend, 422101) before acquisition on BD LSR-II flow cytometer (conventional flow cytometer) or a 5-laser Cytek Aurora

(36) EXTRACELLULAR CIRP INHIBITS NEUTROPHIL APOPTOSIS TO PROMOTE ITS AGING BY UPREGULATING SERPINB2 IN SEPSISShockSeptember 1, 2023Junji Shimizu, Atsushi Murao, Monowar Aziz, Ping Wangprocedure were stained with APC Ly6G Abs. The cells were fixed in Fluorofix buffer (Catalogue No.: 422101; BioLegend) and then permeabilized using perm wash buffer (Catalogue No.: 421002; BioLegend), followed

(37) EXTRACELLULAR CIRP INHIBITS NEUTROPHIL APOPTOSIS TO PROMOTE ITS AGING BY UPREGULATING SERPINB2 IN SEPSISShockJuly 25, 2023Junji Shimizu, Atsushi Murao, Monowar Aziz, Ping Wangprocedure were stained with APC Ly6G Abs. The cells were fixed in Fluorofix buffer (Catalogue No.: 422101; BioLegend) and then permeabilized using perm wash buffer (Catalogue No.: 421002; BioLegend), followed

(38) Proteomic analysis of circulating immune cells identifies cellular phenotypes associated with COVID-19 severityCell reportsJune 27, 2023Potts M, Fletcher-Etherington A, Nightingale K, Mescia F, Bergamaschi L, Calero-Nieto FJ, Antrobus R, Williamson J, Cambridge Institute of Therapeutic Immunology and Infectious Disease-National Institute of Health Research (CITIID-NIHR) COVID BioResource Collaboration, Parsons H, et al.Human TruStain FcX,Biolegend,Cat# 422302 BD Horizon Brilliant Stain Buffer,BD,Cat# 563794 FluoroFix Buffer,BioLegend,Cat# 422101 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, Micro BCA Protein Assay Kit

(39) Mouse Models for Atopic DermatitisCurrent ProtocolsMarch 27, 2023Keiko Sakamoto, Keisuke Nagaofor flow cytometry cannot be done on the same day, fix the cells with FluoroFix™ Buffer (Biolegend cat 422101). After step 9, add 200 μl of FluoroFix™ Buffer into each well and incubate for 30 min at RT. Centrifuge

(40) Anti-cancer effect of targeting fibroblast activation protein alpha in glioblastoma through remodeling macrophage phenotype and suppressing tumor progressionCNS neuroscience & therapeuticsMarch 1, 2023Miao Y, Deng Y, Liu J, Wang J, Hu B, Hao S, Wang H, Zhang Z, Jin Z, Zhang Y, et al.the cells were harvested with 0.05% trypsin, washed with PBS, fixed with FluoroFix Buffer (BioLegend, 422101), incubated with anti‐CD206 (BioLegend, 321106) and anti‐CD163 (BioLegend, 326514) or isotype control

Be the First to Write a Review!

FluoroFix™ Buffer from BioLegend

Description

BioLegend

Citations (62)

Reviews

FluoroFix™ Buffer from BioLegend

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map