EM GP2 Automatic Plunge Freezer from Leica Microsystems

Product Specs
ItemEM GP2 Automatic Plunge Freezer
CompanyLeica Microsystems
Price Get Pricing Get Quote for this Product
Catalog NumberEM GP2
QuantityEA
TypeCryo Preparation System
Temperature Range+4°C to +60°C
ApplicationsVitrification of biological samples for cryo-electron microscopy preparation
Humidity Rangeup to 99%

Specifications/Features

Automatic single sided and multiple sided
Single sided sensor blotting
Fast, easy, and safe filling of the secondary cryogen with the unique liquifying head
Controllable secondary cryogen temperature
Environmental chamber with adjustable temperature and humidity
Intuitive control via touch panel

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Leica Microsystems

10 Parkway North Blvd., Suite 300

Deerfield, Illinois 60015

United States

Phone: 800 248 0123

Company Profile

Website: www.leica-microsystems.com

(1) Paclitaxel compromises nuclear integrity in interphase through SUN2-mediated cytoskeletal couplingJournal of Cell ScienceJune 15, 2026Thomas Hale, Victoria L. Hale, Piotr Kolata, Ália dos Santos, Matteo Allegrettibefore 16 h treatment with 5 nM paclitaxel or control medium. Grids were then transferred to a Leica GP2 plunger at 25°C and 95% humidity using glycerol (10% in PBS) as a cryo-protectant for 20 s. After 9

(2) Structure of giant kelp Photosystem I-FCP uncovers drivers of antenna evolution across the red lineageNat CommunMay 21, 2026Jenevieve D. Weissman, Pablo Maturana, Hui M. O. Oung, Reece Riddle, Gabrielle Wyatt, Viktoria G. T. Dubinin, Philipp Zerbe, María Maldonado10 s, and blotted for 8 s under controlled conditions (18 °C and 90% relative humidity) in a Leica EM GP2 cryo-plunger (Leica, Wetzlar, Germany). Grids were then plunge-frozen in liquid ethane using the same

(3) The clinical ionizable lipid SM-102 enhances photochemical immunogenic cell death by verteporfin-conjugated liposomes and improves survival in vivoPhotochem Photobiol SciMay 12, 2026Nimit Shah, Maxwell Bortei Quaye, Bhavana Sudula, Nikita Kulkarni, Aarati Upreti, Ashritha Malkoochi, Taksheel Rao Aileni, Kunal Karambelkar, Arden Peterson, Doha Mahmoud, et al.grids were vitrified by plunge-freezing into liquid ethane cooled by liquid nitrogen using a Leica GP2 automated plunge freezer (Leica Microsystems). Imaging was performed on either a Talos Arctica or Glacios

(4) A tissue-intrinsic mechanism sensitizes HIV-1 particles for TLR-triggered innate immune responsesNat CommunMay 9, 2026Samy Sid Ahmed, Liv Zimmermann, Andrea Imle, Katrin Wuebben, Nadine Tibroni, Lena Rauch-Wirth, Jan Münch, Petr Chlanda, Frederik Graw, Oliver T. Facklertotal volume of 3 µl was used for plunge freezing into liquid ethane using an automatic plunge freezer EM GP2 (Leica). The ethane temperature was set to −183 °C and the chamber to 24 °C with 80% humidity. Grids

(5) Cryo-HIM-SIMS on the npSCOPE: Correlative Topographic, Transmitted and SIMS Imaging at Cryogenic TemperaturesAnalytical ChemistryMay 5, 2026Tatjana Taubitz, Olivier De Castro, Dustin Andersen, Zahraa Berro, Sukriti Hans, Saba Tabean, Moritz Wachsmuth-Melm, Gerhard Hobler, Inge Nelissen, Falk Lucas, et al.493/503 (Life Technologies D3922, USA). Cells were plunge-frozen in liquid ethane at −183 °C using an EM GP2 plunge-freezer (Leica, Germany) with back-side blotting for 4 s. Grids were inserted into FIB-autogrids

(6) IgG3 Hinge Architecture Expands Dengue Virus Breadth of A Quaternary Epitope Dependent Zika Virus Cross-Neutralizing AntibodybioRxivMay 5, 2026Sanjeev Kumar, Connor A. P. Scott, Lingling Xu, Kathryn Moore, Jacob V. Velden, Samuel Shih, Richard H. West, Sneh Lata Gupta, Naphak Modhiran, Mehul S. Suthar, et al.applied to glow discharged R3.5/1 holey carbon grids (Quantifoil). Grids were then vitrified using an EM GP2 (Leica Microsystems) set at 4 °C and 95% relative humidity, grids were pre-blotted for 10 secs, followed

(7) TFAM organizes DNA into compact higher order structuresbioRxivMay 4, 2026Sashi R. Weerawarana, Wei Tian, Karolin Lugerbinding buffer was added immediately to the grid after wicking. The grid was then transferred to a Leica GP2 set to 90% humidity at 4 °C for plunge freezing. Grids were blotted for 2s and plunge frozen into liquid

(8) Evaluating the Volta phase plate for improved tomogram alignment in cryo-electron tomographyIUCrJMay 1, 2026Joshua Hutchings, Daniel Ji, Shawn Zheng, Elizabeth A. Montabana, Utz H. Ermel, Ariana Peck, Jonathan Schwartz, Rahel Woldeyes, Mohammadreza Paraan, Mallak Ali, et al.Agard’s group at the University of California, San Francisco. Plunge freezing was performed using a Leica GP2 set to 4°C and 95% humidity. The lysate was pipetted up and down (20 strokes) in 10 µl volumes and applied

(9) NV716 acts as an envelope-active adjuvant that enhances antibiotic accumulation in Pseudomonas aeruginosaNPJ Antimicrob ResistApril 30, 2026Margot Draveny, Hugo Chauvet, Axelle De Pauw, Valérie Rouam, Harisa Rista, Pierre Legrand, Marine Novelli, Jean-Michel Bolla, Maria Girleanu, Anne-Laure Favier, et al.before blotting to remove excess media. Membranes were plunge-frozen in liquid ethane using a Leica EM GP2 automatic plunge freezer. Vitrified samples were transferred to a cryo-box in a Leica EM VCM vacuum cryo-manipulation system

(10) Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline modelElifeApril 30, 2026Lingli Kong, Ximena Zottig, Johannes Elferich, Nikolaus Grigorieffsupplemented with 25 mM of HEPES buffer. Grids were then rapidly moved from the incubator to a Leica GP2 cryo-plunger; excess liquid was blotted from the back side for 8 s, and grids were plunge-frozen into

(11) Unbend: Correction of local beam-induced sample motion in cryo-EM images using a 3D spline modeleLifeApril 30, 2026Lingli Kong, Ximena Zottig, Johannes Elferich, Nikolaus Grigorieffsupplemented with 25 mM of HEPES buffer. Grids were then rapidly moved from the incubator to a Leica GP2 cryo-plunger; excess liquid was blotted from the back side for 8 s, and grids were plunge-frozen into

(12) Structural insight into how RAD51 paralog exchange regulates RAD51 filament formationNature Structural & Molecular BiologyApril 22, 2026Yashpal Rawal, Youngho Kwon, Lijia Jia, Eliza A. Ruben, Jae-Hoon Ji, Lijuan Guo, Caleb M. Stratton, Digant Nayak, Miriam Tovar, Qingming Fang, et al.Pelco easiGlow glow discharger before sample application. Frozen grids were prepared using a Leica EM GP2 plunge-freezer with a 3-s blot time at 4 °C and 95% humidity. All grids were transferred into liquid

(13) A robust workflow for 3D imaging of human mitochondria using cryo-electron tomographybioRxivApril 17, 2026Akhil Gargey Iragavarapu, Oleg Artemchuk, Daija Bobe, Anna C. Ratliff, Evgeny Pavlov, Halil Aydina semi-automated plunge freezer, such as the Vitrobot Mark IV System (Thermo Fisher Scientific) or EM GP2 Automatic Plunge Freezer (Leica Microsystems), can freeze samples up to ~10 μm thick, a high pressure freezing (HPF) system can

(14) Diel remodeling and cellular integration of the nitroplastbioRxivApril 17, 2026Ethan H Li, Tyler Coale, Gaëlle Toullec, Artur Czajkowski, Pierre Henri Jouneau, Tim M. Dederichs, Chandni Bhickta, Zachary Raymond, Kyoko Hagino, Vitor Hugo Balasco Serrão, et al.glow-discharged Quantifoil R2/2, 200-mesh grids. The grids were rapidly vitrified in liquid ethane using a Leica GP2 cryo-plunger operated at 85% humidity and 18 °C, with a blotting time of 2 s in sensor blotting mode.

(15) Direct Visualization and Quantitative Analysis of Silica Nanoparticle Dispersion States in Saline Solution via Cryo-TEMACS Measurement Science AuApril 15, 2026Masato Iwasawa, Katsuya Miura, Masaya Kashihara, Hirotake Kitagawa, Masashi Ohno, Daisuke Unabara, Tasuku Hamaguchi, Koji YonekuraFigure S3. Cryo-TEM Measurements and Image Analysis Samples for cryo-TEM were prepared using a LEICA EM GP2 automatic plunge freezer (Leica Microsystems, Germany). Sample aliquots (3 μL) were applied to hydrophilized holey carbon grids

(16) High Drug Encapsulation Capacity in Cyclodextrin-Based Nanoparticles: Characterization and In Vivo Antitumor Efficacy in Tumor-Bearing MiceACS Bio Med Chem AuApril 15, 2026Tomoki Kosugi, Mina Sakuragi, Madoka Toya, Tomoki Nishimura, Taiki Saito, Reina Kobayashi, Liliana de Campo, Kazuo Sakurai, Riku Kawasaki, Noriko Miyamotoonto a carbon grid (Quantifoil, Jena, Germany) and rapidly vitrified in liquid ethane using a Leica EM GP2 plunge freezer (Leica Microsystems, Wetzlar, Germany). The prepared samples were transferred to the

(17) Nano-Flow Cytometry of Single Extracellular Vesicles Reveals Subpopulation Differences Across Cell Types and Pharmacological PerturbationsJ Extracell VesiclesApril 14, 2026Nathalie Nevo, Alix Zhou, Nicolas Ansart, Lea Cohen-Attali, Eric Rubinstein, Coralie Guérin, Lorena Martin-Jaular, Clotilde Thérylacey grids (Ted Pella, USA), blotted from the back side, and flash frozen in liquid ethane with an EM‐GP2 Leica plunger at 90% humidity. Cryo‐EM images were acquired with a Glacios cryo‐electron microscope

(18) Evaluating the Volta phase plate for improved tomogram alignment in cryo-electron tomographyIUCrJApril 9, 2026Joshua Hutchings, Daniel Ji, Shawn Zheng, Elizabeth A. Montabana, Utz H. Ermel, Ariana Peck, Jonathan Schwartz, Rahel Woldeyes, Mohammadreza Paraan, Mallak Ali, et al.Agard’s group at the University of California, San Francisco. Plunge freezing was performed using a Leica GP2 set to 4°C and 95% humidity. The lysate was pipetted up and down (20 strokes) in 10 µl volumes and applied

(19) Microtubules in the axon are GDP bound but adopt a stable GTP-like expanded stateNat Struct Mol BiolApril 8, 2026Elena A. Zehr, Shufeng Sun, Stephanie L. Sarbanes, Antonina Roll-Mecakreactions were blotted with Whatman No. 1 filter paper (Leica Microsystems, 16706440) for 4 s using Leica EM GP2 (Leica Microsystems) with the environmental chamber set to 30 °C and 90% humidity and subsequently plunged

(20) In situ chromatin dynamics and HIV-1 nuclear traffickingbioRxivApril 3, 2026Zhen Hou, Yao Shen, Long Chen, Juan Shen, David I. Stuart, Peijun Zhangand Fiji (ImageJ)72. Plunge-freezing vitrification Sample vitrification was performed using a Leica EM GP2 automated plunge freezer under controlled conditions (20 °C, 95% relative humidity). The mixture of

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