EM ICE High Pressure Freezer from Leica Microsystems

Product Specs
ItemEM ICE High Pressure Freezer
CompanyLeica Microsystems
Price Get Pricing Get Quote for this Product
Catalog NumberEM ICE
QuantityEA
TypeHigh Pressure Freezer
Dimensions(WxDxH) 116 x 79 x 128.5 cm
CapacityHold up to 3 different specimens
ApplicationsCryo-fixation of biological samples for electron microscopy ultrastructure preservation

Specifications/Features

Programmable sequential freezing of nine (3 × 3) samples
Automated LN2 re-filling of the sample storage dewar
Recovery time between freezing cycles is one minute
Retrofitable light stimulation and/or electrical stimulation mode

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Leica Microsystems

10 Parkway North Blvd., Suite 300

Deerfield, Illinois 60015

United States

Phone: 800 248 0123

Company Profile

Website: www.leica-microsystems.com

(1) Damselflies overcome color saturation barriers of photonic glasses via pigment loading and refractive index modulationProceedings of the National Academy of SciencesJune 9, 2026Tali Lemcoff, Lotem Alus, Albert Batushansky, Yahel Fishman, Nila Theodor, Keshet Shavit, Lahav Hyitner, Almut Kelber, Johannes S. Haataja, Dan Oron, et al.9004-54-0, Sigma-Aldrich) in 1X PBS solution and cryo-immobilized in a high-pressure freezing device (EM ICE, Leica). The frozen samples were then mounted on a holder under liquid nitrogen in a specialized loading

(2) Cell fusion after wounding requires plasma membrane damage and endocytosisbioRxivJune 2, 2026Junmin Hua, Evan S. Krystofiak, Andrew D. Pumford, Andrea Page-McCaw, M. Shane Hutsontransitioning them to 30% glycerol in 50 mM HEPES over 24 hours. Samples were vitrified using a Leica EM-ICE high-pressure freezer using two B hats for maximum depth. After vitrification, samples were freeze substituted in 1% uranyl

(3) Characterization Standard for In-situ Cryo-electron TomographybioRxivMay 26, 2026Mallak Ali, Joshua Hutchings, Tahiti Dutta, Nikki Jean, Garret Greenan, Elizabeth A. Montabana, Jonathan Schwartz, M.G. Finn, Matthias Haury, David A. Agard, et al.acquisition. Dataset 25oct20a was collected on plunge-frozen grids. High-pressure freezing: a Leica EM ICE high-pressure freezer was used to establish the waffle protocol12,13. To achieve a high concentration of cells, the pellet

(4) Cryo‐FIB Lift‐Out and Electron Tomography Workflow for Bacteria–Nanopillar Interface Imaging Under Native Conditions: Investigating Dragonfly Inspired Bactericidal Titanium SurfacesSmall MethodsMay 21, 2026Chaturanga D. Bandara, Dominik Pinkas, Martina Zanova, Martin Uher, Judith Mantell, Bo Su, Angela H. Nobbs, Paul Verkadenitrogen until imaging. 2.4.2 High‐Pressure Freezing High‐pressure freezing was performed using a Leica EM ICE system. Samples at room temperature were added with 50 µL of E. coli suspension for 20 min in 3 mm copper

(5) In situ architecture of plasmodesmata in Physcomitrium patens resolved by cryo-electron tomographyNat PlantsMay 14, 2026Marcel Dickmanns, Matthias Pöge, Peng Xu, Sven Gombos, Zoe K. Barr, Manuel Miras, Jürgen M. Plitzko, Rüdiger Simon, Waltraud X. Schulze, Wolf B. Frommer, et al.dissolved in H2O to a final concentration of 20% (w/v)) and immediately high-pressure frozen (LEICA EM ICE, Leica Mikrosysteme GmbH). Gametophores were collected after four weeks by manually dissecting individual

(6) A versatile nanobody platform for live and super-resolution imaging of synaptic vesicle dynamics and plasticity in rodent and human neuronsJ NanobiotechnologyMay 14, 2026Rashi Goel, Kristina Jevdokimenko, Ronja Rehm, Jannik Hentze, Paola Agüi-Gonzalez, Momchil Ninov, Erik Maier, Yin Wu, Felix Lange, Agata Witkowska, et al.then underwent washing steps in prewarmed (37 °C) conditioned medium before rapid cryofixation via an EM ICE high-pressure freezer (Leica) and storage in liquid nitrogen. Semiautomated freeze-substitution was performed via an AFS2

(7) Electron microscopy‐based three‐dimensional subcellular imaging of plant male gametophyteJournal of Integrative Plant BiologyMay 1, 2026Zhiqi Liu, Zizhen Liang, Mengfei Liao, Yixin Huang, Rui Ma, Jiayang Gao, Weiqi Wang, Tao Ni, Philipp S. Erdmann, Liwen Jianggrains Freshly collected Arabidopsis anthers of mixed developing stages were subjected to HPF (Leica EM ICE) with 0.15 M sucrose as cryo‐protectant in type B aluminum HPF carrier (Leica #16770142), followed by

(8) Built different: ER cisternae formed by the Arabidopsis Lunapark proteins differ in ultrastructure and affect ER –Golgi transportNew PhytologistApril 29, 2026Charlotte Pain, Tatiana Spatola Rossi, Nadine Field, Carmen Mata, Alessia Candeo, Muhammad W. Ali, Flavia Moreira‐Leite, Stanley W. Botchway, Federica Brandizzi, Verena KriechbaumerFluid AG). Fixation of tissue for the electron tomography of cisternae was performed using HPF in an EM ICE (Leica Microsystems, Wetzlar, Germany). Samples were then transferred to an EM AFS2 freeze substitution

(9) Diel remodeling and cellular integration of the nitroplastbioRxivApril 17, 2026Ethan H Li, Tyler Coale, Gaëlle Toullec, Artur Czajkowski, Pierre Henri Jouneau, Tim M. Dederichs, Chandni Bhickta, Zachary Raymond, Kyoko Hagino, Vitor Hugo Balasco Serrão, et al.membrane using a manual vacuum filtration unit, and allowed to sediment prior to high-pressure freezing (EM-ICE, Leica Microsystems). A 1.2 μl aliquot of the concentrated mix was transferred into a type A carrier

(10) Large-scale endoplasmic reticulum membrane solidification spatially organises proteins under thermal or metabolic stressbioRxivApril 12, 2026Paul M. Mueller, Melissa R. Mikolaj, Elmehdi Belbaraka, Finn Hartstein, Sophia Altinoluk, Bjoern Perder, Philipp Trnka, Robert-William Welke, Heike Naumann, Nina Taudien, et al.ethanol series, and embedded in the PolyBed® 812 resin (Polysciences). High pressure freezing (Leica EM ICE) of cell monolayers on sapphire discs was followed by freeze substitution (Leica AFS2) in 1% (w/v) osmium

(11) RNA promotes synapsin phase separation providing a platform for local translationbioRxivApril 11, 2026Branislava Rankovic, Zachary M Geisterfer, Akshita Chhabra, Vladimir M Jovanovic, Ian Seim, Christian Hoffmann, Antonela Condric, Gerard Aguilar Aguilar Pérez, Rinse de Boer, Lars T. Hofstede, et al.PHEM buffer, which served as a cryoprotectant. Cells were cryo-fixed using a high-pressure freezer (EM ICE; Leica). Freeze substitution was performed in acetone supplemented with 0.2% uranyl acetate and 2% H₂O

(12) Macrophage anti-bacterial activity is controlled by adenylate kinase 4-mediated mitochondrial DNA synthesisJ Exp MedApril 6, 2026Wei-Yao Chin, Ching-Tung Wu, Gunn-Guang Liou, Si-Tse Jiang, Yi-Sheng Cheng, Jr-Shiuan Lin, Betty A. Wu-Hsieh, Shi-Chuen Miawafter BMDMs were infected with Listeria for 24 h, cells were harvested, then were cryo-fixed by Leica EM ICE, dehydrated in a series of cold methanol treatments, and infiltrated serially with solutions of embedding

(13) SNX-mediated biogenesis of a plant-unique vesicle derived from the multivesicular bodyNature CommunicationsMarch 24, 2026Yanbin Li, Ran Tao, Hai Zhang, Xiaokang Wen, Stephen King Pong Leung, Qing Qi, Xiaohui Zheng, Haoxuan Guo, Congxian Wu, Zhifei Fu, et al.5-day-old Arabidopsis root tips were excised and rapidly frozen using a high-pressure freezing apparatus (EM ICE, Leica). For ultrastructural analysis, freeze substitution was performed using 2% OsO₄ and 0.5% uranyl

(14) A proof-of-concept study on high pressure freezing for cryopreservationPNAS NexusMarch 20, 2026Fang Song, Mayuko Sato, Yuya Toyama, Taiyo Ishikawa, Fumiya Tokito, Takeshi Katsuda, Kiminori Toyooka, Yasuyuki Sakai, Masaki Nishikawaotherwise lead to damage or artifacts. We used an HPF device commonly employed for cryofixation, the Leica EM ICE. After mounting the carrier onto the specimen holder, vitrification can be achieved with a one-click

(15) Visualizing suborganellar lipid distribution using correlative light and electron microscopyNature Cell BiologyMarch 20, 2026H. Mathilda Lennartz, Suman Khan, Weihua Leng, Kristin Böhlig, Gunar Fabig, Yannick Kieswald, Falk Elsner, Nadav Scher, Michaela Wilsch-Bräuninger, Ori Avinoam, et al.their respective dilutions. High-pressure freezing High-pressure freezing was performed using a Leica EM-ICE (Leica Microsystems) or a Wohlwend Compact 03 (Wohlwend GmbH). Before high-pressure freezing, cells

(16) Comparison of Crosslinking Reagents and Their Impacts on Bone Derived ECM HydrogelsAdvanced Healthcare MaterialsMarch 19, 2026Joshua N. Jones, I‐Ning Lee, Andreas Rialas, Simon C. Kellaway, Rabea Loczenski, Christopher Parmenter, Lisa J. Whitewith manual baseline corrections. 4.5.2 Electron microscopy Samples were prepared using the Leica EM ICE high pressure freezer (HPF) (Leica, Austria). Central sections of gel samples were removed with a 3 mm tissue punch and placed

(17) An Advanced Mobile Laboratory to enable field-based microbial ecology and cell biology across scalesbioRxivMarch 16, 2026N Leisch, S Baars, T Beavis, P Bertucci, C Bhickta, M Bonadonna, C M Brannon, L Burgués-Palau, P Cherek, F Chevalier, et al.sandwiched with the flat side of the type B aluminium carrier (Wohlwend) and high-pressure frozen with the EM ICE (Leica Microsystems). The samples were freeze-substituted at EMBL’s Electron Microscopy Core Facility

(18) Novel PMVs/ZIP4/Zinc/Prelamin A Axis Promotes Nuclear Dysmorphism and Vascular Aging in Humans and Rodents Post-Injury: Effective Treatment With Platelet Membrane-Coated ZIF-8 NanoparticlesAging CellMarch 9, 2026Tengzhi Ma, Han Bao, Zhijue Xu, He Ren, Wenhao Tian, Jiahe Chen, Zhongqian Liu, Xinwu Lu, Fan Lv, Qingping Yao, et al.ultramicrotomy. For high‐pressure freezing‐based TEM preparation, VSMCs were cryo‐fixed using a Leica EM ICE high‐pressure freezer (Leica Microsystems, Germany), ensuring millisecond vitrification and optimal preservation of ultrastructural

(19) CLCC1 governs ER bilayer equilibration to maintain lipid homeostasisNatureFebruary 25, 2026Lingzhi Wu, Jianqin Wang, Yawei Wang, Junhan Yang, Yuanhang Yao, Yonglun Wang, Dong Huang, Yating Hu, Xinxuan Xu, Renqian Wang, et al.carrier. The assembly of the sandwich carrier was promptly inserted into the high-pressure cryostat (Leica EM ICE) for vitrification. The vitrified sandwich assembly was then transferred to a liquid ethane and propane

(20) Structural and functional implications of phase separation of membrane protein LacY in Escherichia coliNature CommunicationsFebruary 25, 2026Dmitrii Linnik, Sumayra Sultanji, Jan A. Stevens, Gea K. Schuurman-Wolters, Rinse de Boer, Christiaan M. Punter, Siewert J. Marrink, Ivan Maslov, Bert Poolmancopper gold-plated type B (flat-surfaced) carrier (Leica). Cells immobilized by high-pressure freezing (EM ICE, Leica) were freeze-substituted in 1% (w/v) OsO4 plus 0.5% uranyl acetate in acetone with 5% water,

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Leica EM ICE

EM ICE High Pressure Freezer from Leica Microsystems

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Specifications/Features

Leica Microsystems

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EM ICE High Pressure Freezer from Leica Microsystems

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