EM ICE High Pressure Freezer from Leica Microsystems

Product Specs
ItemEM ICE High Pressure Freezer
CompanyLeica Microsystems
Price Get Pricing Get Quote for this Product
Catalog NumberEM ICE
QuantityEA
TypeHigh Pressure Freezer
Dimensions(WxDxH) 116 x 79 x 128.5 cm
CapacityHold up to 3 different specimens
ApplicationsCryo-fixation of biological samples for electron microscopy ultrastructure preservation

Specifications/Features

Programmable sequential freezing of nine (3 × 3) samples
Automated LN2 re-filling of the sample storage dewar
Recovery time between freezing cycles is one minute
Retrofitable light stimulation and/or electrical stimulation mode

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Leica Microsystems

10 Parkway North Blvd., Suite 300

Deerfield, Illinois 60015

United States

Phone: 800 248 0123

Company Profile

Website: www.leica-microsystems.com

(1) Large-scale endoplasmic reticulum membrane solidification spatially organises proteins under thermal or metabolic stressbioRxivApril 12, 2026Paul M. Mueller, Melissa R. Mikolaj, Elmehdi Belbaraka, Finn Hartstein, Sophia Altinoluk, Bjoern Perder, Philipp Trnka, Robert-William Welke, Heike Naumann, Nina Taudien, et al.ethanol series, and embedded in the PolyBed® 812 resin (Polysciences). High pressure freezing (Leica EM ICE) of cell monolayers on sapphire discs was followed by freeze substitution (Leica AFS2) in 1% (w/v) osmium

(2) Macrophage anti-bacterial activity is controlled by adenylate kinase 4-mediated mitochondrial DNA synthesisJ Exp MedApril 6, 2026Wei-Yao Chin, Ching-Tung Wu, Gunn-Guang Liou, Si-Tse Jiang, Yi-Sheng Cheng, Jr-Shiuan Lin, Betty A. Wu-Hsieh, Shi-Chuen Miawafter BMDMs were infected with Listeria for 24 h, cells were harvested, then were cryo-fixed by Leica EM ICE, dehydrated in a series of cold methanol treatments, and infiltrated serially with solutions of embedding

(3) Visualizing suborganellar lipid distribution using correlative light and electron microscopyNature Cell BiologyMarch 20, 2026H. Mathilda Lennartz, Suman Khan, Weihua Leng, Kristin Böhlig, Gunar Fabig, Yannick Kieswald, Falk Elsner, Nadav Scher, Michaela Wilsch-Bräuninger, Ori Avinoam, et al.their respective dilutions. High-pressure freezing High-pressure freezing was performed using a Leica EM-ICE (Leica Microsystems) or a Wohlwend Compact 03 (Wohlwend GmbH). Before high-pressure freezing, cells

(4) A proof-of-concept study on high pressure freezing for cryopreservationPNAS NexusMarch 20, 2026Fang Song, Mayuko Sato, Yuya Toyama, Taiyo Ishikawa, Fumiya Tokito, Takeshi Katsuda, Kiminori Toyooka, Yasuyuki Sakai, Masaki Nishikawaotherwise lead to damage or artifacts. We used an HPF device commonly employed for cryofixation, the Leica EM ICE. After mounting the carrier onto the specimen holder, vitrification can be achieved with a one-click

(5) Comparison of Crosslinking Reagents and Their Impacts on Bone Derived ECM HydrogelsAdvanced Healthcare MaterialsMarch 19, 2026Joshua N. Jones, I‐Ning Lee, Andreas Rialas, Simon C. Kellaway, Rabea Loczenski, Christopher Parmenter, Lisa J. Whitewith manual baseline corrections. 4.5.2 Electron microscopy Samples were prepared using the Leica EM ICE high pressure freezer (HPF) (Leica, Austria). Central sections of gel samples were removed with a 3 mm tissue punch and placed

(6) An Advanced Mobile Laboratory to enable field-based microbial ecology and cell biology across scalesbioRxivMarch 16, 2026N Leisch, S Baars, T Beavis, P Bertucci, C Bhickta, M Bonadonna, C M Brannon, L Burgués-Palau, P Cherek, F Chevalier, et al.sandwiched with the flat side of the type B aluminium carrier (Wohlwend) and high-pressure frozen with the EM ICE (Leica Microsystems). The samples were freeze-substituted at EMBL’s Electron Microscopy Core Facility

(7) Novel PMVs/ZIP4/Zinc/Prelamin A Axis Promotes Nuclear Dysmorphism and Vascular Aging in Humans and Rodents Post-Injury: Effective Treatment With Platelet Membrane-Coated ZIF-8 NanoparticlesAging CellMarch 9, 2026Tengzhi Ma, Han Bao, Zhijue Xu, He Ren, Wenhao Tian, Jiahe Chen, Zhongqian Liu, Xinwu Lu, Fan Lv, Qingping Yao, et al.ultramicrotomy. For high‐pressure freezing‐based TEM preparation, VSMCs were cryo‐fixed using a Leica EM ICE high‐pressure freezer (Leica Microsystems, Germany), ensuring millisecond vitrification and optimal preservation of ultrastructural

(8) CLCC1 governs ER bilayer equilibration to maintain lipid homeostasisNatureFebruary 25, 2026Lingzhi Wu, Jianqin Wang, Yawei Wang, Junhan Yang, Yuanhang Yao, Yonglun Wang, Dong Huang, Yating Hu, Xinxuan Xu, Renqian Wang, et al.carrier. The assembly of the sandwich carrier was promptly inserted into the high-pressure cryostat (Leica EM ICE) for vitrification. The vitrified sandwich assembly was then transferred to a liquid ethane and propane

(9) Structural and functional implications of phase separation of membrane protein LacY in Escherichia coliNature CommunicationsFebruary 25, 2026Dmitrii Linnik, Sumayra Sultanji, Jan A. Stevens, Gea K. Schuurman-Wolters, Rinse de Boer, Christiaan M. Punter, Siewert J. Marrink, Ivan Maslov, Bert Poolmancopper gold-plated type B (flat-surfaced) carrier (Leica). Cells immobilized by high-pressure freezing (EM ICE, Leica) were freeze-substituted in 1% (w/v) OsO4 plus 0.5% uranyl acetate in acetone with 5% water,

(10) Encephalopathy-linked UFM1 variants impede neuronal protein translation, development, and functionEMBO Molecular MedicineFebruary 23, 2026Catarina Perdigão, Josefa Torres, Helge M Magnussen, Janina Koch, Elena Rudashevskaya, Frederieke Moschref, Maksims Fiosins, Fritz Benseler, Sally Wenger, Tanja Nilsson, et al.CaCl2, 140 mM NaCl, 2.4 mM KCl, 10 mM Glucose, 10 mM HEPES, pH 7.6) before rapid cryofixation using an EM ICE high-pressure freezer (Leica) and subsequent storage in liquid nitrogen. Semi-automated freeze-substitution was performed

(11) Structural Insights into Cell Wall-Related Vesicle Secretion with Different MechanismsPlants (Basel)February 22, 2026Jiawen Yang, Sheng Chang, Linlin Li, Xiaoyu Ma, Hua Han, Zheng Liu, Jian Zhufreeze substitution. Yeast cell samples were cultured as previously described [44,45] and prepared using a Leica EM ICE (Leica Microsystems, Wetzlar, Germany) high-pressure freezing and a Leica EM AFS2 freeze substitution

(12) A Glucan Synthase-Remodeler Module Organizes Branched Glucan Assembly in the Fungal Cell WallbioRxivFebruary 21, 2026Alaina H. Willet, Anand Jacob, Lesley A. Turner, Abdulrahman Khalid A. Alsanad, Tuo Wang, Kathleen L. Gouldwere loaded into 3 mm high-pressure freezer specimen carriers (Ted Pella) and vitrified using a Leica EM ICE high-pressure freezer. Samples were freeze-substituted in 1.5% uranyl acetate in methanol at −90°C for 48 hours followed by

(13) Unraveling design principles of protein landscapes in photosynthetic membranes in plant chloroplastsSci AdvFebruary 13, 2026Ashraf Mohamed, Yuval Garty, Hui Min Olivia Oung, Sujith Puthiyaveetil, Kajwal K. Patra, Andrea Tagliabue, Steven Lenhert, Dana Charuvi, Reinat Nevo, Helmut Kirchhoff, et al.aluminum discs (Engineering Office M. Wohlwend, Switzerland). Samples were high-pressure frozen with EM-ICE (Leica Microsystems, Austria). Freeze fracture was conducted at −120°C using a BAF 060 (Leica Microsystems,

(14) Gene expression analysis reveals distinct PHB depolymerization mechanisms and broader involvement of the PHB cycle in Rhodospirillum rubrum growing on acetate and fructoseMicrobial Cell FactoriesFebruary 9, 2026Hugo Fleuriot-Blitman, Katerina Sabatova, Véronique Amstutz, Marketa Jakubickova, Eva Slaninova, Katerina Mrazova, María Celeste Ferrín Mendoza, Kamila Hrubanova, Vladislav Krzyzanek, Stanislav Obruca, et al.chloroform, and covered with the flat side of type B carriers. Samples were rapidly vitrified in the EM ICE high-pressure freezer (Leica Microsystems, Austria). Frozen carriers were transferred under liquid nitrogen into a freeze-substitution

(15) Ultrastructural membrane dynamics of mouse and human cortical synapsesNeuronFebruary 4, 2026Chelsy R. Eddings, Minghua Fan, Yuuta Imoto, Kie Itoh, Xiomara McDonald, Jens Eilers, William S. Anderson, Paul F. Worley, Kristina Lippmann, David W. Nauen, et al.Arrow indicates vibratome cut direction.(B) A zap board enabling electrical stimulation in the Leica EM ICE high-pressure freezer.(C) Overlay image of a 6 mm wide rubber O-ring, two sapphire disks and a Mylar spacer ring in between

(16) ER remodelling is a feature of ageing and depends on ER-phagyNature Cell BiologyFebruary 2, 2026Eric K. F. Donahue, Nathaniel L. Hepowit, Elizabeth M. Ruark, Alexandra G. Mulligan, Brennen Keuchel, Nicholas D. Urban, Li Peng, Stedman Stephens, Derek J. Johnson, Natalie S. Wallace, et al.the flat side of B-type carrier (Leica) and vitrified using a high-pressure freezing machine (Leica EM ICE). The frozen specimens were stored in liquid nitrogen until further processing with a modified freeze-substitution

(17) Energy metabolism and adaptation to hypoxia in the non-photosynthetic green alga LeontynkaBMC BiologyJanuary 29, 2026Pia Corre, Jana Pilátová, Tomáš Bílý, Eliška Zadrobílková, Ivan Čepička, Marie Vancová, Martin Lohr, Oliver D. Caspari, Marek Eliáš, Tomáš Pánekresulting from thermal isomerization. Electron microscopy Pelleted cells were high-pressure frozen (EM ICE, Leica) in the presence of 20% BSA and were freeze-substituted in 2% OsO4 in 100% acetone for 96 h at

(18) PAC regulates endo-, and exocytosis, and lysosomal-mitochondrial stress signaling in human mast cellsCell Communication and SignalingJanuary 20, 2026Korollus Melek, Philip Steiner, Sven Kappel, Benjamin Gottschalk, Ancuela Andosch, Michelle Duggan, Wolfgang F. Graier, Susanna Zierler, Christine PeineltMicrosystems, Vienna, Austria) for high-pressure freeze fixation (HPF). HPF was performed using a Leica EM ICE device (Leica Microsystems, Vienna, Austria) to achieve a cooling rate of at least 17,500 °C per second

(19) Structural and Functional Characterization of EXPO‐Derived Extracellular Vesicles in PlantsAdvanced ScienceJanuary 12, 2026Jiayang Gao, Yanbin Li, Shengqi Zhang, Yilin He, Zhiqi Liu, Juan Wang, Jingmin Hua, Jinyu Chen, Junru Zhong, Huan Zhong, et al.and leaves from 5‐day‐old Arabidopsis seedlings were harvested, cut, and rapidly frozen using a Leica EM ICE High‐Pressure Freezer then transferred to the Leica AFS system. The samples are submerged in acetone with 0.25% glutaraldehyde

(20) Correlating Molecular and Textural Properties of Raw Soy‐Based and Beef Burgers Using TD‐NMR and TPAJournal of Food ScienceJanuary 9, 2026Moshe Hai Azachi, Zeev Wiesmancryo‐immobilized between two aluminum discs (3 mm diameter, 200 µm thick each) using a high‐pressure freezer (EM ICE, Leica). Sample preparation and imaging followed this workflow: Transferred under liquid nitrogen to

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Leica EM ICE

EM ICE High Pressure Freezer from Leica Microsystems

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Specifications/Features

Leica Microsystems

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EM ICE High Pressure Freezer from Leica Microsystems

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