DpnI from New England Biolabs

Product Specs
ItemDpnI
CompanyNew England Biolabs
Price $294.00 Inquire
Catalog NumberR0176L
Concentration20 u/µL
Quantity5000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

Ga/TC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Minimal indel-inducing and window-adjustable base editing using inactive Cas12b effectorsMolecular Therapy Nucleic AcidsDecember 8, 2026Wi-jae Lee, Yun Gyeong Kim, Yeounsun Oh, Lee Wha Gwon, Dongjae Lim, Ho-Joong Kim, Bo-Woong Sim, Seung Hwan LeeQIAquick PCR Purification Kit (28106; Qiagen). The purified products (29 μL) were mixed with 1 μL of DpnI (R0176L; New England Biolabs), 5 μL of rCutSmart buffer (50 mM potassium acetate, 20 mM Tris acetate, 10 mM

(2) Protocol for Cas9 nickase-mediated induction and two-dimensional gel detection of i-loops at human telomeric and alpha satellite repeatsSTAR ProtocOctober 6, 2026Elia Zanella, Flavia Bruno, Ylli Doksani10297018 Doxycycline hydrochloride,Sigma-Aldrich,Cat# D9891; CAS: 24390-14-5 DpnI restriction enzyme,NEB,Cat# R0176L "DTT (dithiothreitol), 1 M",Sigma-Aldrich,Cat# D9779 EDTA,Sigma-Aldrich,Cat# E9884 EGTA,Sigma-Aldrich,Cat#

(3) A novel p53R273H-Selective Bispecific T-Cell Engager: from Clinical TCR clone to Computational Optimization and Functional ValidationMolecular Therapy OncologySeptember 17, 2026Nhan Huynh, Thu-My Thi Nguyen, Ngoc Thi Bui, Thao-Nguyen Ho Nguyen, Diem T.P. Tran, Hoai-Nghia Nguyen, Cuong Thach NguyenQ5 High Fidelity DNA polymerase (NEB M0491S) or GXL DNA polymerase (Takara R050A), followed by DpnI (NEB R0176L) treatment and isopropanol-based PCR cleanup. These fragments were then inserted into pcDNA 3.4 (+)

(4) Minigene-based characterization and classification of splice-associated variants in succinate dehydrogenase BNPJ Precis OncolSeptember 8, 2026Anni Köhler, Alexandra A. Baumann, Natasha Lewis, Anja Richter, Susan Richter, Doreen William, Andrés Cruz-García, Hanno Glimm, Stefan Fröhling, Diana Le Duc, et al.(c.424-151T>G, c.201-14T>G, c.232A>T), conditions were adapted56. PCR products were treated with DpnI (NEB, R0176L) at 37 °C for 1 h to remove methylated parental plasmid DNA and transformed into DH5α cells (NEB, C29871)

(5) Histone H3K9 methylation and Heterochromatin Protein 1 do not limit DNA accessibility in living S. pombe cellsbioRxivSeptember 5, 2026Lalita Panigrahi, Zhuwei Xu, David J. Clarkpaired-end library preparation Genomic DNA was digested with 10 U DpnI (New England Biolabs (NEB) R0176L) at 37°C for 1.5 h. The reaction was terminated by adding 10 mM EDTA, 0.1% SDS. DpnI-digested DNA was

(6) Barcoded Rabies In Situ Connectomics for high-throughput reconstruction of neural circuitsNature CommunicationsSeptember 2, 2026Alexander Becalick, Antonin Blot, Molly Strom, Petr Znamenskiyfollowing reaction mix: 0.5 μl EagI-HF (New England Biolabs, R3505L), 0.25 μl DpnI (New England Biolabs, R0176L), 500 ng linear barcoded DNA, 5 μl CutSmart buffer (New England Biolabs, B6004S) and molecular-grade

(7) Protocol for connector-enabled multigene pathway assembly using Golden Gate Assembly and yeast recombinationSTAR ProtocolsSeptember 1, 2026Min-Jun Seong, Jonghyeok Shin, Dae-Hee LeeDimethyl sulfoxide (DMSO),Sigma-Aldrich,Cat# 472301 Dropout supplement -LEU (DO supplement -LEU),Takara Bio,Cat# 630414 DpnI,NEB,Cat# R0176L Gibson Assembly Master Mix,NEB,Cat# E2611 ,, Critical commercial assays,Critical commercial assays,Critical

(8) Deubiquitination of Vangl by USP6 and USP32 Regulates Planar Cell Polarity SignalingAdvanced ScienceAugust 30, 2026Fangzi Zha, Di Feng, Ziru Xue, Zhiying Liu, Xiaochen Lin, Zhe Wang, Ling Xu, Jasper Fuk Woo Chan, Martin Cheung, Michael Shing Yan Huen, et al.methodologies. Site‐directed mutagenesis was implemented by PCR amplification coupled with DpnI (NEB, R0176L) digestion. pRK5‐HA‐Ubiquitin‐K6R, 11R, 27R, 29R, 33R, 48R, 63R plasmids and pCMV‐3×FLAG‐RNF43 were

(9) Quantifying protein unfolding kinetics with a high-throughput microfluidic platformCell SystemsJuly 29, 2026Beatriz Atsavapranee, Fanny Sunden, Daniel Herschlag, Polly M. FordyceKit,New England Biolabs,E6800L RNAsin Ribonuclease Inhibitor,Promega,N2515 DpnI,New England Biolabs,R0176L QIA Prep spin miniprep kit,QiaGen,27104 ,, Deposited Data,Deposited Data,Deposited Data Raw unfolding

(10) The adaptive landscapes of three global Escherichia coli transcriptional regulatorsElifeJuly 21, 2026Cauã Antunes Westmann, Leander Goldbach, Andreas Wagner,Quick CIP,New England Biolabs,–,Dephosphorylation Commercial assay or kit,DpnI,New England Biolabs,Cat# R0176L,Template removal before sequencing Commercial assay or kit,Exonuclease I,New England Biolabs,Cat# M0293L,ssDNA

(11) Mechanism of ribosome stalling by the AMD1 C-terminal tail arrest peptideSci AdvMarch 27, 2026Emir Maldosevic, Fabio S. Boiocchi, Michal I. Swirski, Kyle A. Meiklejohn, Martina M. Yordanova, Pavel V. Baranov, Ahmad Jomaa(New England Biolabs, catalog no. M0491L). For IVA cloning, Dpn I (New England Biolabs, catalog no. R0176L) digestion was performed to remove parental DNA templates. All constructs were transformed into Escherichia coli

(12) Human LFA-1 governs T cell immune surveillance of the skinScience immunologyFebruary 27, 2026Yatim A, Youssefian L, Idani A, Mourelatos H, Alipour Olyaei N, Habibi L, Peel JN, Gaballa M, Kim C, Lee D, et al.50-196-5299). After amplification, PCR products were digested with DpnI (New England Biolabs, catalog no. R0176L) at 37°C for 1 hour for selective degradation of the parental plasmid. The resulting nicked plasmids

(13) Integrating natural and engineered genetic variations to decode regulatory influence on blood traitsCell ReportsFebruary 24, 2026Manuel Tardaguila, Dominique Von Schiller, Michela Colombo, Ilaria Gori, Eve L. Coomber, Thomas Vanderstichele, Paola Benaglio, Chiara Chiereghin, Sebastian Gerety, Dragana Vuckovic, et al.T4 DNA ligase,New England Biolabs,Cat#M0202L NaeI,New England Biolabs,Cat#R0190S DpnI,New England Biolabs,Cat#R0176L BamHI-HF,New England Biolabs,Cat#R3136L KpnI-HF,New England Biolabs,Cat#R3142L Shrimp Phosphatase,New England Biolabs

(14) Integrins mediate symbiont-specific uptake in cnidarian larvaeEMBO ReportsDecember 16, 2025Victor A S Jones, Melanie Dörr, Isabelle Siemers, Sebastian Rupp, Sami El Hilali, Sara Brites, Joachim M Surm, Ira Maegele, Sebastian G Gornik, Meghan Ferguson, et al.(B0202S; NEB), 1 µL T4 Polynucleotide Kinase (M0201S; NEB), 1 µL T4 ligase (M0202S; NEB), 1 µL DpnI (R0176L; NEB), and 5 µL H2O, and incubated for 1 h at 37 °C. Half the reaction mix was transformed into chemically

(15) Synthetic closed-loop gene circuit for phenylalanine regulationNucleic Acids ResearchNovember 4, 2025Silvia Galvan, Yu-Qing Xie, Ana P Teixeira, Martin Fusseneggerpolymerase chain reaction (PCR)-amplified using primers that had ~20 bp complementary sequences at each end, digested with DpnI (R0176L, New England Biolabs) and cloned utilizing a custom mix of enzymes. To perform mutagenesis PCR, primers

(16) Interactions between the genome and the nuclear lamina are multivalent and cooperativeNature Structural & Molecular BiologyNovember 1, 2025Lise Dauban, Mathias Eder, Marcel de Haas, Vinícius H. Franceschini-Santos, J. Omar Yañez-Cuna, Moreno Martinovic, Tom van Schaik, Christ Leemans, Hans Teunissen, Koen Rademaker, et al.follows. Genomic DNA was isolated (BIO-52067, Bioline) and ~500 ng was digested with DpnI (10 U; NEB, R0176L) in CutSmart Buffer 1× (8 h at 37 °C and 20 min at 80 °C) in a total volume of 10 µl. Poly(A) tailing

(17) Engineering wetware and software for the predictive design of compressed genetic circuits for higher-state decision-makingNat CommunOctober 24, 2025Brian D. Huang, Yongjoon Yu, Junghwan Lee, Matthew W. Repasky, Yao Xie, Matthew J. Realff, Corey J. Wilsonmixture composed of T4 polynucleotide kinase (NEB M0201L), T4 DNA ligase (NEB M0202L), and DpnI (NEB R0176L), and then transformed into chemically competent NEB® 10-beta cells. More than 1000 colonies were scraped

(18) Mammalian and Bacterial Adaptors Function as Co-Disinhibitory Pairs to Activate the E3 ubiquitin ligase WWP2Journal of Biological ChemistryOctober 22, 2025Samkeliso V. Blundell, Mei Liu, Romina Tocci, Andrea Majstorovic, Solène Tsang, David W. Holdenreagents","Chemicals, enzymes, and other reagents","Chemicals, enzymes, and other reagents" DpnI,NEB,R0176L BsmBI-V2,NEB,R0739S BbsI,NEB,R0539S HindIII-HF,NEB,R3104S NotI- HF,NEB,R3189S NcoI-HF,NEB,R3193S Foetal

(19) An AI-driven workflow for the accelerated optimization of cell-free protein synthesisiScienceOctober 17, 2025Mostafa M. Khalil, Aisha Elsawah, An N. Hoang, Jean-Loup Faulon, Baptiste Panthu, Joan Hérissonsequences. All restriction enzymes used were sourced from NEB. After each digestion, a DpnI treatment (NEB #R0176L) was performed for 1 h to degrade any residual template DNA, followed by purification using the NEB

(20) Systematic discovery of CRISPR-boosted CAR T cell immunotherapiesNatureSeptember 24, 2025Paul Datlinger, Eugenia V. Pankevich, Cosmas D. Arnold, Nicole Pranckevicius, Jenny Lin, Daria Romanovskaia, Moritz Schaefer, Francesco Piras, Anne-Christine Orts, Amelie Nemc, et al.CROP-seq-CAR-multi vectors (7,622 bp, including 40-bp extensions), followed by DpnI digestion (NEB, R0176L) to remove the parental plasmid. Sequences for the CARs were amplified from the corresponding CROP-seq-CAR

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(1)

1/6/2023Plasmid Removal With DPNI Scarlette CASSALI use this enzyme to remove plasmid template after PCR, before the cloning step. Cloning works very well this way.

DpnI

DpnI from New England Biolabs

Sequence

New England Biolabs

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DpnI from New England Biolabs

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