DpnI from New England Biolabs

Product Specs
ItemDpnI
CompanyNew England Biolabs
Price $294.00 Inquire
Catalog NumberR0176L
Concentration20 u/µL
Quantity5000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

Ga/TC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Mechanism of ribosome stalling by the AMD1 C-terminal tail arrest peptideSci AdvMarch 27, 2026Emir Maldosevic, Fabio S. Boiocchi, Michal I. Swirski, Kyle A. Meiklejohn, Martina M. Yordanova, Pavel V. Baranov, Ahmad Jomaa(New England Biolabs, catalog no. M0491L). For IVA cloning, Dpn I (New England Biolabs, catalog no. R0176L) digestion was performed to remove parental DNA templates. All constructs were transformed into Escherichia coli

(2) Integrating natural and engineered genetic variations to decode regulatory influence on blood traitsCell ReportsFebruary 24, 2026Manuel Tardaguila, Dominique Von Schiller, Michela Colombo, Ilaria Gori, Eve L. Coomber, Thomas Vanderstichele, Paola Benaglio, Chiara Chiereghin, Sebastian Gerety, Dragana Vuckovic, et al.T4 DNA ligase,New England Biolabs,Cat#M0202L NaeI,New England Biolabs,Cat#R0190S DpnI,New England Biolabs,Cat#R0176L BamHI-HF,New England Biolabs,Cat#R3136L KpnI-HF,New England Biolabs,Cat#R3142L Shrimp Phosphatase,New England Biolabs

(3) Integrins mediate symbiont-specific uptake in cnidarian larvaeEMBO ReportsDecember 16, 2025Victor A S Jones, Melanie Dörr, Isabelle Siemers, Sebastian Rupp, Sami El Hilali, Sara Brites, Joachim M Surm, Ira Maegele, Sebastian G Gornik, Meghan Ferguson, et al.(B0202S; NEB), 1 µL T4 Polynucleotide Kinase (M0201S; NEB), 1 µL T4 ligase (M0202S; NEB), 1 µL DpnI (R0176L; NEB), and 5 µL H2O, and incubated for 1 h at 37 °C. Half the reaction mix was transformed into chemically

(4) Synthetic closed-loop gene circuit for phenylalanine regulationNucleic Acids ResearchNovember 4, 2025Silvia Galvan, Yu-Qing Xie, Ana P Teixeira, Martin Fusseneggerpolymerase chain reaction (PCR)-amplified using primers that had ~20 bp complementary sequences at each end, digested with DpnI (R0176L, New England Biolabs) and cloned utilizing a custom mix of enzymes. To perform mutagenesis PCR, primers

(5) Interactions between the genome and the nuclear lamina are multivalent and cooperativeNature Structural & Molecular BiologyNovember 1, 2025Lise Dauban, Mathias Eder, Marcel de Haas, Vinícius H. Franceschini-Santos, J. Omar Yañez-Cuna, Moreno Martinovic, Tom van Schaik, Christ Leemans, Hans Teunissen, Koen Rademaker, et al.follows. Genomic DNA was isolated (BIO-52067, Bioline) and ~500 ng was digested with DpnI (10 U; NEB, R0176L) in CutSmart Buffer 1× (8 h at 37 °C and 20 min at 80 °C) in a total volume of 10 µl. Poly(A) tailing

(6) Engineering wetware and software for the predictive design of compressed genetic circuits for higher-state decision-makingNat CommunOctober 24, 2025Brian D. Huang, Yongjoon Yu, Junghwan Lee, Matthew W. Repasky, Yao Xie, Matthew J. Realff, Corey J. Wilsonmixture composed of T4 polynucleotide kinase (NEB M0201L), T4 DNA ligase (NEB M0202L), and DpnI (NEB R0176L), and then transformed into chemically competent NEB® 10-beta cells. More than 1000 colonies were scraped

(7) Mammalian and Bacterial Adaptors Function as Co-Disinhibitory Pairs to Activate the E3 ubiquitin ligase WWP2Journal of Biological ChemistryOctober 22, 2025Samkeliso V. Blundell, Mei Liu, Romina Tocci, Andrea Majstorovic, Solène Tsang, David W. Holdenreagents","Chemicals, enzymes, and other reagents","Chemicals, enzymes, and other reagents" DpnI,NEB,R0176L BsmBI-V2,NEB,R0739S BbsI,NEB,R0539S HindIII-HF,NEB,R3104S NotI- HF,NEB,R3189S NcoI-HF,NEB,R3193S Foetal

(8) An AI-driven workflow for the accelerated optimization of cell-free protein synthesisiScienceOctober 17, 2025Mostafa M. Khalil, Aisha Elsawah, An N. Hoang, Jean-Loup Faulon, Baptiste Panthu, Joan Hérissonsequences. All restriction enzymes used were sourced from NEB. After each digestion, a DpnI treatment (NEB #R0176L) was performed for 1 h to degrade any residual template DNA, followed by purification using the NEB

(9) Systematic discovery of CRISPR-boosted CAR T cell immunotherapiesNatureSeptember 24, 2025Paul Datlinger, Eugenia V. Pankevich, Cosmas D. Arnold, Nicole Pranckevicius, Jenny Lin, Daria Romanovskaia, Moritz Schaefer, Francesco Piras, Anne-Christine Orts, Amelie Nemc, et al.CROP-seq-CAR-multi vectors (7,622 bp, including 40-bp extensions), followed by DpnI digestion (NEB, R0176L) to remove the parental plasmid. Sequences for the CARs were amplified from the corresponding CROP-seq-CAR

(10) Coordinated control of genome-nuclear lamina interactions by topoisomerase 2B and lamin B receptorNucleic acids researchSeptember 23, 2025van Schaik T, Magnitov M, de Haas M, Breda J, de Wit E, Manjon AG, Medema RH, Gothe HJ, Roukos V, Buckle AJ, et al.protocol: genomic DNA was isolated (Bioline, BIO-52067) and ∼500 ng were digested with DpnI (10 U, NEB, R0176L) in CutSmart Buffer 1× (8 h 37°C, 20 min 80°C) in a total volume of 10 μl. A-tailing was performed by

(11) A population model reveals a surprising role of stochastic cell division in epigenetic memory systemsiScienceSeptember 19, 2025Viviane Klingel, Dimitri Graf, Sara Weirich, Albert Jeltsch, Nicole E. Raddeproteins","Chemicals, peptides, and recombinant proteins" ,, Restrictionenzyme DpnI,New England Biolabs,"Cat# R0176L, Lot:10227051" Restrictionenzyme EcoRI-HF,New England Biolabs,Cat# R3101L; Lot:0151708 ,, Critical

(12) Dynamic association of H3K36me3 with pericentromeric heterochromatin regulates its replication timeEMBO ReportsSeptember 8, 2025Sunil Kumar Pradhan, Hui Zhang, Ksenia G Kolobynina, Alexander Rapp, Maria Arroyo, M Cristina CardosoGmbH, Frankfurt am Main, Germany",M0202L DpnI,"New England Biolabs GmbH, Frankfurt am Main, Germany",R0176L DMEM high glucose,"Sigma-Aldrich Chemie GmbH, Steinheim, Germany",D6429 1× non-essential amino acids,"Sigma-Aldrich

(13) Pharmacological Targeting of DHHC9‐Mediated STRN4 Palmitoylation to Suppress YAP‐Driven Cancer MetastasisJournal of Cellular and Molecular MedicineSeptember 3, 2025Yang Tian, Wei Li, Qing Zhai, Ying Yu, Jiaxin Yuan, Yan Ma, Jingjing Yang, Mingyue Li, Wenwen Chang, Wenjing Li, et al.U.S.","12,100,046" DNA ladder (1 kb),"Abclonal, China",RM19005 dNTP mix,"New England Biolabs, U.S.",R0176L Doxycycline,"MCE, U.S.",HY‐N0565 DpnI,"New England Biolabs, U.S.",R0176S DTDP,"Sigma, U.S.",D5767 EcoRI‐HF,"New England Biolabs

(14) Lung and liver editing by lipid nanoparticle delivery of a stable CRISPR-Cas9 ribonucleoproteinNature biotechnologySeptember 1, 2025Chen K, Han H, Zhao S, Xu B, Yin B, Lawanprasert A, Trinidad M, Burgstone BW, Murthy N, Doudna JA.(New England Biolabs (NEB), M0530L). After PCR, the reactions were treated with 1 μl of DpnI (NEB, R0176L) for 1 h at 37 °C before gel purification. The plasmids were ligated by Gibson assembly (NEB master

(15) Heterozygous variants in PLCG1 affect hearing, vision, cardiac, and immune functionElifeAugust 27, 2025Mengqi Ma, Yiming Zheng, Mingxi Deng, Shenzhao Lu, Xueyang Pan, Xi Luo, Michelle Etoundi, David Li-Kroeger, Kim C Worley, Lindsay C Burrage, et al.strategy using Q5 Hot Start High-Fidelity 2x Master Mix (NEB, #M0494S) and DpnI restriction enzyme (NEB, # R0176L). Human PLCG2 cDNA was obtained from Genescript (clone ID OHu24072) and was cloned into pUAST vector

(16) Mutations elevate an underground pathway to a physiologically relevant protopathwayMol Biol EvolAugust 7, 2025Karl A Widney, Lauren C Phillips, Leo M Rusch, Shelley D Copleypurification kit (T1130L) and the template plasmid was removed by restriction digestion with NEB DpnI (R0176L) following the manufacturer's protocol. Amplicons of the correct size were purified using a GeneJET

(17) MBD2 promotes B cell differentiation and BCR signaling in systemic lupus erythematosus by regulating the LEF-1-PTEN-PI3K axisCell Death DisJune 4, 2025Yukai Jing, Yunfei Zhang, Xiaocui Wang, Yufen Wang, Ying Hu, Bin Wen, Xin Dai, Xuemei Duan, Haonan Li, Shumin Dong, et al.precipitation was used to precipitate the PCR products, which were then treated with 1.5 μL of DpnI (R0176L) from NEB with incubation at 37 °C for 2 h. Next, 3 μL of digested PCR products were transformed into

(18) Structural insights into lipid membrane binding by human ferlinsThe EMBO JournalMay 28, 2025Constantin Cretu, Aleksandar Chernev, Csaba Zoltán Kibédi Szabó, Vladimir Pena, Henning Urlaub, Tobias Moser, Julia PreobraschenskiPhusion High-Fidelity DNA polymerase,New England Biolabs,Cat#M0530S Restriction enzymes,New England Biolabs,SspI-HF (cat#R3132L)DpnI (cat#R0176L) T4 DNA Polymerase (LIC-qualified),Novagen,cat#70099 T4 DNA Ligase,Thermo Fisher Scientific,cat#EL0011 Purified

(19) Nucleosome dynamics render heterochromatin accessible in living human cellsNat CommunMay 16, 2025Hemant K. Prajapati, Zhuwei Xu, Peter R. Eriksson, David J. Clarkguidelines. Purified genomic DNA (1.2 - 1.5 µg) was digested with 10 units of DpnI (New England Biolabs (NEB) R0176L) in NEB CutSmart buffer for 2 h at 37 °C. RNA-seq About 0.5 million MCF7 cells were transduced using

(20) MLX phosphorylation stabilizes the ChREBP-MLX heterotetramer on tandem E-boxes to control carbohydrate and lipid metabolismSci AdvMarch 14, 2025Carla E. Cadena Del Castillo, Onur Deniz, Femke van Geest, Lore Rosseels, Ingrid Stockmans, Marius Robciuc, Sebastien Carpentier, Bettina K. Wölnerhanssen, Anne Christin Meyer-Gerspach, Ralph Peterli, et al.introduced by reverse polymerase chain reaction (PCR; table S1), followed by Dpn I (New England Biolabs, R0176L) digestion and bacterial transformation. For GSK3 KO, sgRNAs targeting GSK3α (table S1) were cloned

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(1)

1/6/2023Plasmid Removal With DPNI Scarlette CASSALI use this enzyme to remove plasmid template after PCR, before the cloning step. Cloning works very well this way.

DpnI

DpnI from New England Biolabs

Sequence

New England Biolabs

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DpnI from New England Biolabs

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