Wizard SV Gel and PCR Clean-Up System from Promega

Product Specs
ItemWizard SV Gel and PCR Clean-Up System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA9281
Quantity50 preps
Range100bp to 10kb from standard or low-melt agarose gels
Methodfrom agarose gels in either TAE or TBE buffer.
Sample TypeGels, PCR, enzymatic reactions
Timelittle as 15 minutes
SourceReaction, Gel
Nucleic Acid TypeDNA Fragment, PCR Product

Specifications/Features

% Recovery:
70–95% recovery of fragments

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Knockout of filamin A in KGN granulosa tumor cells impairs proliferation, cell cycle progression, migration, and cytoskeletal organization under mechanical stressBiol ResMay 20, 2026Yuhao Jiang, Karolina Magdalena Caban, Mirko Peitzsch, Carola Herrmann, Doris Mayr, Jan Bernd Stöckl, Thomas Fröhlich, Artur Mayerhofer, Annette Müller-Taubenberger, Harald WelterNew England Biolabs, Germany). Target bands were purified using the Wizard® SV Gel and PCR Clean-Up System (#A9281, Promega, USA) according to the manufacturer’s instructions and subjected to sequencing. Determination

(2) Improvement of chicken genome editing efficiency in vitro using ribonucleoprotein-mediated CRISPR/Cas9 deliveryJ Anim Sci BiotechnolMay 4, 2026Yujin Han, Chan Young Kwon, Jae Yong Hangel electrophoresis, each amplicon was purified using the Wizard™ SV Gel and PCR Clean-Up System (#A9282, Promega, WI, USA). Next-generation sequencing analysis Next-generation sequencing (NGS) was outsourced

(3) GLYATL1 is associated with metabolic and epigenetic changes and with endocrine resistance in luminal breast cancerClin EpigeneticsApril 29, 2026Janina Müller, Emre Sofyali, Luisa Schwarzmüller, Yael Aylon, Eviatar Weizman, Lisa Schlicker, Katherine Kelly, Simone Borgoni, Simin Oz, Cole Stocker, et al.agarose gel electrophoresis and bands were gel-purified using the Wizard SV Gel and PCR Clean-up System (#A9281, Promega, Madison, WI, USA), according to the manufacturer’s instructions. Sanger-sequencing was performed

(4) Genome-wide identification and solute selectivity of aquaporins in the sharpnose sevengill shark, Heptranchias perloPhysiol RepApril 24, 2026Shinichiro Hidaka, Ayumi Nagashima, Akira Kato1166B), and purified using the Wizard SV Gel and PCR Clean‐Up System (Promega, Madison, WI, USA; Cat. No. A9282). Capped sense RNA (cRNA) was synthesized from the linearized Aqp expression vector using the mMessage mMachine T7 kit

(5) Leptin Acts as a Peripheral Tropic Signal to Tune SteroidogenesisbioRxivApril 20, 2026Jie Sun, Adam Aldahir, Wen-Kan Liu, Calder Ellsworth, Xian-Feng Wang, Hong-Cun Bao, Yi-Chun Huang, Fenglei He, Christoph Heier, Wu-Min Dengon a 0.8% agarose gel at 90 V for 45 min and isolated using the Wizard SV Gel Clean-Up System (Cat#: A9281, Promega, Madison, WI) according to the manufacturer’s instructions. Subsequently, the restriction products

(6) Topographic structure and function of locus coeruleus norepinephrine neuronsbioRxivApril 10, 2026Zhixiao Su, Polina Kosillo, Kanghoon Jung, Shuonan Chen, Mathew T. Summers, Alex Piet, Han Hou, Kenta M. Hagihara, Drew Friedmann, Olivia Ho-Shing, et al.reaction volume. The PCR2 products were cleaned up and concentrated by SV wizard PCR cleanup kit (Promega, A9282) and loaded into a 2% agarose gel for electrophoresis. The 233 bp PCR product was cut from the agarose

(7) Transcriptomic and RNA Modification Landscape of Severe Fever with Thrombocytopenia Syndrome Virus Revealed by Nanopore Direct RNA SequencingMicroorganismsMarch 27, 2026Haowen Yuan, Bohan Zhang, Ling Qiu, Jingwan Han, Lei Jia, Xiaolin Wang, Yongjian Liu, Hanping Li, Hongling Wen, Lin Liagarose gel electrophoresis purification kit (Wizard® SV Gel and PCR Clean-Up System, Promega, Madison, WI, USA, A9282), followed by in vitro transcription using the T7 High Yield RNA Transcription Kit (Vazyme, Nanjing,

(8) Microalgal colony blot: A simple and rapid method for direct detection of recombinant protein production in microalgae coloniesPLoS OneMarch 25, 2026Yasin Torres-Tiji, Francis J. Fields, Stephen P. Mayfieldfirst digested with KpnI and XbaI, then purified using a Wizard® SV Gel and PCR Clean-Up System (Cat. #A9281, Promega, Madison, WI) and eluted in ultrapure DNase/RNase-free H₂O to prevent salt interference during

(9) Glomerular basement membrane structural integrity dictates trans-tissue deposition of laminin in the kidneyCell ReportsMarch 24, 2026Kohei Omachi, Meei-Hua Lin, Pongpratch Puapatanakul, Joshua F. Begin, Karen K. McKee, Hironobu Fujiwara, Peter D. Yurchenco, Jeffrey H. Minergel-purified to remove plasmid vector sequences using the Wizard SV Gel and PCR Clean-Up System (Promega, #A9281), and further purified by ethanol precipitation. The purified transgene was microinjected into the pronuclei

(10) C9orf72 hexanucleotide repeat RNA drives transcriptional dysregulation through genome-wide DNA:RNA hybrid G-quadruplexesNeuronMarch 18, 2026Honghe Liu, Mingming Liu, Yang Liu, Gege Gui, Tapas Paul, Yu-Ning Lu, Zhiyuan Huang, Haocheng Wang, Yatao Xiao, Zhongfan Zheng, et al.reporter gene. The resulting DNA was purified with a Wizard SV Gel and PCR Clean-Up system (Promega, A9281) and applied to the HeLaScribe Nuclear Extract in vitro Transcription System (Promega, E3091). In brief,

(11) Brachiopod genome unveils the evolution of BMP signalling in bilaterian body patterningNature CommunicationsMarch 12, 2026Thomas D. Lewin, Tosuke Sakagami, Keisuke Shimizu, Li-Jung Kao, Yi-Ling Chiu, Isabel Jiah-Yih Liao, Mu-En Chen, Kanako Hisata, Kazuyoshi Endo, Noriyuki Satoh, et al.agarose TAE gel, and target bands were purified using the Wizard SV Gel and PCR Clean-Up System (Promega A9282). Primer sequences for probe synthesis are provided in Supplementary Data 40. In vitro transcription

(12) HIV broadly neutralizing antibody escape dynamics drive the outcome of AAV-vectored immunotherapy in humanized miceImmunityMarch 10, 2026Nicolas M.S. Galvez, Adam D. Nitido, Seo Bin Yoo, Yi Cao, Cailin E. Deal, Christine L. Boutros, Scott W. MacDonald, Yentli E. Soto Soto Albrecht, Evan C. Lam, Maegan L. Sheehan, et al.Hot Start Master Mix (NEB, Cat#M0493L), the PCR product was purified by gel extraction (Promega, Cat#A9282) and cloned by homologous recombination into the appropriate recipient parental backbone vector using

(13) CXCR4-tropic HIV-1 infection in an immunocompetent monkey modelNature CommunicationsMarch 9, 2026Nicholas R. Meyerson, Vanessa L. Bauer, Will T. Fattor, Cody J. Warren, Arturo Barbachano-Guerrero, Melisa J. Weiss, Obaiah Dirasantha, Bridget L. Burris, Adetunji S. Adesina, Joseph L. Timpona, et al.nested set of amplicons were gel-extracted using the Wizard SV Gel and PCR Clean-Up System (Promega #A9281) and the resulting products were diluted 1:100 in nuclease-free water before being used as templates

(14) Structure and function of persulfide dioxygenase from Pseudomonas aeruginosa: Implications on H2S homeostasis and interplay with nitric oxideiScienceFebruary 20, 2026Francesca Giordano, Francesca Troilo, Martina Roberta Nastasi, Lorenzo Caruso, Marta Mellini, Carlo Travaglini-Allocatelli, Giorgio Giardina, João B. Vicente, Giordano Rampioni, Adele Di Matteo, et al.assays ,, Pierce™ BCA Protein Assay Kits,Thermo Scientific,23226 Wizard® SV Gel and PCR Clean UP system,Promega,A9282 Wizard® Plus SV Minipreps DNA Purification System,Promega,A1460 T4 DNA ligase (Ligase and Buffer),Promega,M1801 Hydrogen

(15) Evolutionary and Modification Features of Two Monkeypox Virus Strains: Insights from Integrated Genomic and Epigenomic AnalysesVirusesFebruary 18, 2026Zhongru Zhao, Bohan Zhang, Jingwan Han, Dandan Lin, Yongjian Liu, Lei Jia, Hanping Li, Jingyun Li, Xiaolin Wang, Hongling Wen, et al.confirmed by 1% agarose gel electrophoresis and purified using the Wizard® SV Gel and PCR Clean-Up System (A9282) (Promega Corporation, Madison, WI, USA). The purified 59 fragments were accurately quantified using

(16) Global genetic rewiring during compensatory evolution in the yeast polarity networkEMBO ReportsFebruary 16, 2026Enzo Kingma, Marieke Glazenburg, Karel Olavarria, Liedewij LaanUltra II DNA Library Prep Kit,NovoGene,N/A Wizard® SV Gel and PCR Clean-Up System,Promega Corporation,A9281 Reagents and tools table

(17) Genomic analysis of Ostreococcus tauri-infecting viruses reveals a hypervariable region associated with host-virus interactionsVirus EvolFebruary 11, 2026Julie Thomy, Julien Henri, David Demory, Frederic Sanchez, Marie-Line Escande, Gilles Mirambeau, Nigel Grimsley, Sheree YauSanger sequencing, PCR products were purified using a Wizard® SV gel and PCR clean-up system kit (Promega, ref A9281). Sequences were cleaned by manually trimming low-quality base pairs from the 3′ and 5′ ends using Geneious

(18) Unrecognized high prevalence of expanded composite repeats in Friedreich ataxiaHuman Molecular GeneticsFebruary 10, 2026Morgan C Devore, Christina Lam, Graham Wiley, Courtney C Park, David R Lynch, Sanjay I Bidichandaniamplicons were purified for longread sequencing using the Wizard SV Gel and PCR Clean-Up System (Promega; A9282). Detection of the proximal FXN deletion The recurrent proximal FXN deletion was detected by PCR using

(19) Injury-induced electrochemical coupling triggers organ growthSci AdvFebruary 6, 2026Jinghui Liu, Elisa Nerli, Charlie Duclut, Amit S. Vishen, Naomi Berbee, Sylvia Kaufmann, Cesar Ponce, Aristides B. Arrenberg, Frank Jülicher, Rita Mateus2494-bp amplified products were purified using a polymerase chain reaction (PCR) cleanup kit (Promega, #A9281) and recombined with pDONOR221 [Tol2kit, #208 (98)] in a BP reaction (BP Clonase II enzyme mix, Invitrogen,

(20) Essentiality and dynamic expression of the human tRNA pool during viral infectionMolecular Systems BiologyJanuary 20, 2026Noa Aharon-Hefetz, Michal Schwartz, Einav Aharon, Noam Stern-Ginossar, Orna Dahan, Yitzhak Pilpelsoftware,"Li et al, 2014", Other,Other,Other Illumina NovaSeq 1.5B,Illumina, PCR clean-up kit,Promega,A9281 NucleoBond Xtra Midi kit,Macherey-Nagel,740412.50 Monarch Spin High-Capacity DNA cleanup kit,NEB,T1135S Wizard

Write a Review

(1)

9/13/2017DNA Purification Gulshan SinghI am working in the field of environmental microbiology. I used this kit for the purification of my PCR product before cloning and also purify DNA after restriction digestion.
(1)

2/6/2013Wizard SV Gel and PCR Clean-Up System from Promega M.A. NavarreteThe Wizard SV Gel and PCR Clean-Up System performed very well; it was fast, reliable and inexpensive. The protocol was simple and resulted in high quality DNA, as evidenced by spectrophotometry ... Read Review

Wizard® SV Gel and PCR Clean-Up System From Promega
Molecular cloning procedures often require decent amounts of pure DNA fragments, usually generated ... Read More