PUREBLU™ DAPI from Bio-Rad (AbD Serotec - FAB)

Product Specs
ItemPUREBLU™ DAPI
CompanyBio-Rad
Price $139.00Supplier Page View Company Product Page
Catalog Number1351303
Absorbance / Excitation359/461 nm
Purity>95% (HPLC purified)
Molecular Weight350.25
FormulaC₁₆H₁₅N₅·2HCl
TemperatureStable for 2 years at –20°C
After resuspension: stable for 1 year at –20°C or 6 months at 2–8°C
ApplicationsFlow Cytometry,Immunofluorescence
Quantity250 µg
FormatReagent
TypeAccessory Reagent

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) CTCF regulates wild-type and recombinant AAV gene expression by shaping viral chromatinbioRxivJune 3, 2026Clairine I.S. Larsen, Rhiannon R. Abrahams, Rea Guertler, Elliott M. Wilion, Eda Erata, Zachary H. Sykes, Lorelei Stoica, Gopishankar Thirumoorthy, Divya Sinha, Richa Rai, et al.were washed once in PBS prior to being resuspended in PBS with 1ug/ul DAPI live/dead stain (BioRad; 1351303). Cells were immediately put on ice till FACs analysis. Samples were measured on the ThermoFisher Attune NxT

(2) Gene supplementation with precise transgene expression rescues hearing loss in a mouse model with an Mpzl2 East Asian founder variantMolecular TherapyJanuary 7, 2026Seung Hyun Jang, Hyeong Gi Song, Sun Young Joo, Jung Ah Kim, Se Jin Kim, Jae Young Choi, Jinsei Jung, Heon Yung Gee1:1,000 dilution in a blocking buffer. During the secondary antibody incubation, DAPI (1:100, catalog no. 1351303; Bio-Rad) and Alexa Fluor 488 phalloidin (1:400, catalog no. A12379; Invitrogen) were co-incubated when

(3) DNA damaging agents boost the transcription of endothelin A receptor in high-grade serous ovarian cancerJournal of Experimental & Clinical Cancer ResearchDecember 6, 2025Celia Roman, Rosanna Sestito, Valentina Caprara, Andrea Sacconi, Giovanni Blandino, Anna Bagnato, Piera Tocciand goat anti-rabbit Alexa Fluor 488 for pSTAT3. DAPI (PureBlu DAPI nuclear staining dye, cat. no. 1351303, Bio-Rad) was used to stain the nuclei. The samples were visualized in a Leica DMIRE2 deconvolution

(4) Homophilic wiring principles underpin neuronal network topology in vitroElifeJuly 8, 2025Danyal Akarca, Alexander WE Dunn, Philipp J Hornauer, Silvia Ronchi, Michele Fiscella, Congwei Wang, Marco Terrigno, Ravi Jagasia, Petra E Vértes, Susanna B Mierau, et al.temperature. After washing with PBS, sections were incubated with PureBlu DAPI (Bio-Rad, Hercules, CA, USA, #1351303) for 3 min and mounted with ProLong Gold antifade mounting medium (Thermo Scientific, #P36930). Fluorescence

(5) Homophilic wiring principles underpin neuronal network topology in vitroElifeJuly 8, 2025Danyal Akarca, Alexander W E Dunn, Philipp J Hornauer, Silvia Ronchi, Michele Fiscella, Congwei Wang, Marco Terrigno, Ravi Jagasia, Petra E Vértes, Susanna B Mierau, et al.temperature. After washing with PBS, sections were incubated with PureBlu DAPI (Bio-Rad, Hercules, CA, USA, #1351303) for 3 min and mounted with ProLong Gold antifade mounting medium (Thermo Scientific, #P36930). Fluorescence

(6) A hypomorphic model of CPS1 deficiency for investigating the effects of hyperammonemia on the developing nervous systemDisease Models & MechanismsJuly 1, 2025Stuti Bakshi, Taryn Diep, Brandon J. Willis, Rachel Reyes, Grace F. Wu, Georgios Makris, Martin Poms, Isabel Day, Qin Sun, Irina Zhuravka, et al.glutamine synthetase antibody (Thermo Fisher Scientific, A-11001, used at 1:500) and PureBlu DAPI (Bio-Rad, 1351303, used at 1:500)]. After washes, sections were mounted with ProLong Gold Antifade Mountant (Thermo Fisher Scientific

(7) Axon-specific microtubule regulation drives asymmetric regeneration of sensory neuron axonsElifeFebruary 24, 2025Ana Catarina Costa, Blanca R Murillo, Rita Bessa, Ricardo Ribeiro, Tiago Ferreira da Silva, Patrícia Porfírio-Rodrigues, Gabriel G Martins, Pedro Brites, Matthias Kneussel, Thomas Misgeld, et al.algorithm",GraphPad Prism 8,GraphPad,RRID:SCR_002798, Other,PureBlu DAPI Nuclear Staining Dye,Bio-Rad,Cat# 1351303,"Highly pure formulation of DAPI (4',6-diamidino-2-phenylindole), a well-characterized blue-emitting

(8) Axon-specific microtubule regulation drives asymmetric regeneration of sensory neuron axonsElifeFebruary 24, 2025Ana Catarina Costa, Blanca R Murillo, Rita Bessa, Ricardo Ribeiro, Tiago Ferreira da Silva, Patrícia Porfírio-Rodrigues, Gabriel G Martins, Pedro Brites, Matthias Kneussel, Thomas Misgeld, et al.algorithm",GraphPad Prism 8,GraphPad,RRID:SCR_002798, Other,PureBlu DAPI Nuclear Staining Dye,Bio-Rad,Cat# 1351303,"Highly pure formulation of DAPI (4',6-diamidino-2-phenylindole), a well-characterized blue-emitting

(9) α-Synuclein triggers cofilin pathology and dendritic spine impairment via a PrP(C)-CCR5 dependent pathwayCell death & diseaseApril 13, 2024Oliveira da Silva MI, Santejo M, Babcock IW, Magalhães A, Minamide LS, Won SJ, Castillo E, Gerhardt E, Fahlbusch C, Swanson RA, et al.0.1% Sudan Black in 70% ethanol for 10 min at RT. Sections were washed, incubated with DAPI (Bio-Rad, 1351303) for 10 min and mounted in ibidi mounting medium (ibidi, 50001). For staining of αSyn pS129, mouse brain

(10) Tau mediates the reshaping of the transcriptional landscape toward intermediate Alzheimer's disease stagesFrontiers in cell and developmental biologyJanuary 1, 2024Siano G, Varisco M, Terrigno M, Wang C, Scarlatti A, Iannone V, Groth M, Galas MC, Hoozemans JJM, Cellerino A, et al.room temperature. Following a PBS-T wash, the slices were incubated with PureBlu DAPI (Bio-Rad, Cat# 1351303) for 3 min before being mounted with ProLong Gold antifade mounting media (Thermo Fisher Scientific,

(11) Differences in Radiosensitivity According to EGFR Mutation Status in Non-Small Cell Lung Cancer: A Clinical and In Vitro StudyJournal of Personalized MedicineDecember 25, 2023Hidekazu Tanaka, Masako Karita, Kazushi Ueda, Taiki Ono, Miki Kajima, Yuki Manabe, Koya Fujimoto, Yuki Yuasa, Takehiro Shiinoki(ab150077) (abcam, Cambridge, UK) as the secondary antibody, and DAPi (PureBlu DAPI Nuclear Staining Dye #1351303) (BIO-RAD, Hercules, CA, USA) for nuclear staining. The number of γH2AX foci at each time point was

(12) Activation of multiple Eph receptors on neuronal membranes correlates with the onset of optic neuropathyEye and VisionOctober 2, 2023Thomas A. Strong, Juan Esquivel, Qikai Wang, Paul J. Ledon, Hua Wang, Gabriel Gaidosh, David Tse, Daniel Pelaezfor 2 h at room temperature. We used 4′, 6-diamidino-2-phenylindole (DAPI) (1 µg/mL; Bio-Rad, Cat.# 1351303) to counterstain for confocal microscopy. The sectioned tissues were mounted on microscope slides with

(13) Activation of Multiple Eph Receptors on Neuronal Membranes Correlates with The Onset of Traumatic Optic NeuropathybioRxiv : the preprint server for biologyJune 7, 2023Strong TA, Esquivel J, Wang Q, Ledon PJ, Wang H, Gaidosh G, Tse D, Pelaez D.2 hours at room temperature. We used 4’, 6-diamidino-2-phenylindole (DAPI) (1 ug/mL; Bio-Rad, Cat.# 1351303) to counterstain for confocal microscopy. The sectioned tissues were mounted on microscope slides with

(14) A novel NONO variant that causes developmental delay and cardiac phenotypesScientific ReportsJanuary 18, 2023Toshiyuki Itai, Atsushi Sugie, Yohei Nitta, Ryuto Maki, Takashi Suzuki, Yoichi Shinkai, Yoshihiro Watanabe, Yusuke Nakano, Kazushi Ichikawa, Nobuhiko Okamoto, et al.Alexa 568 (A32723, ThermoFisher) at 400-fold dilution and with PureBlu DAPI Nuclear Staining Dye (#1351303; Bio-Rad, USA) at 100-fold dilution and 4 °C overnight. Finally, it was washed three times with 0.3%

(15) Alzheimer's disease biomarker profiling in a memory clinic cohort without common comorbiditiesBrain communicationsJanuary 1, 2023Daniilidou M, Eroli F, Alanko V, Goikolea J, Latorre-Leal M, Rodriguez-Rodriguez P, Griffiths WJ, Wang Y, Pacciarini M, Brinkmalm A, et al.conjugated secondary goat anti-rabbit and anti-mouse antibodies (Thermo Fisher Scientific) and 1:100 DAPI (1351303; Bio-Rad, USA). To quench the autofluorescence of lipofuscin, sections were washed for 5 min in PBS

(16) Gentle Isolation of Nuclei from the Brain Tissue of Adult African Turquoise Killifish, a Naturally Short-Lived Model for Aging ResearchJournal of Visualized ExperimentsAugust 29, 2022Bryan B. Teefy, Ari Adler, Rajyk Bhala, Bérénice A. Benayoun(50,000U)",Lucigen,30281-2, Propidium Iodide solution,MBL,FP00010020, PureBlu™ DAPI Nuclear Staining Dye,Biorad,1351303, "TipOne RPT Pipette Tips (Ultra low retention, filtered) in 10, 20, 200 and 1000μL sizes",USA Scientific,#1181-3810;

(17) α-Synuclein fibril-specific nanobody reduces prion-like α-synuclein spreading in miceNature communicationsJuly 19, 2022Butler YR, Liu Y, Kumbhar R, Zhao P, Gadhave K, Wang N, Li Y, Mao X, Wang W.anti-p129S primary antibody (1:1000 dilution, Abcam, Cat no. ab51253) and DAPI (1:1,000, Bio-Rad, Cat no. 1351303) for 1 h at RT. The secondary stainings were performed with anti-rabbit IgG-AlexaFluor 568 (1:2000 dilution,

(18) The Prognostic Value of the Circulating Tumor Cell-Based Four mRNA Scoring System: A New Non-Invasive Setting for the Management of Bladder CancerCancersJune 25, 2022Amantini C, Maggi F, Rossi de Vermandois JA, Gubbiotti M, Giannantoni A, Mearini E, Nabissi M, Tomassoni D, Santoni G, Morelli MB.IgG Abs (Alexa Fluor® 594, 1:100, Abcam, Cambridge, UK) were used to stain the CTCs. PureBluTM DAPI (#1351303, BioRad) labeled the nuclei. C2 Plus confocal laser scanning microscope (Nikon Instruments, Firenze,

(19) Derivation and Characterization of Murine and Amphibian Müller Glia Cell LinesTranslational vision science & technologyApril 1, 2022Gallo RA, Qureshi F, Strong TA, Lang SH, Pino KA, Dvoriantchikova G, Pelaez D.temperature. We used 4’, 6-diamidino-2-phenylindole (1 µg/mL; Bio-Rad Laboratories, Hercules, CA; #1351303) to counterstain. Specificity of labeling was confirmed by omitting the primary antibody. Imaging was

(20) Correlation between High PD-L1 and EMT/Invasive Genes Expression and Reduced Recurrence-Free Survival in Blood-Circulating Tumor Cells from Patients with Non-Muscle-Invasive Bladder CancerCancersNovember 28, 2021Morelli MB, Amantini C, Rossi de Vermandois JA, Gubbiotti M, Giannantoni A, Mearini E, Maggi F, Nabissi M, Marinelli O, Santoni M, et al.goat anti-mouse IgG H&L Ab (Alexa Fluor® 594, 1:100, Abcam, Cambridge, UK), labeled with PureBlu™ DAPI (#1351303, BioRad) according to the manufacturer’s protocol. Slides were then analyzed under 60X magnification

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(1)

6/30/2016PureBlu DAPI Nuclear Stain Evan HermelPureBlu is a very easy to use reagent for nuclear staining during fluorescence microscopy.

PUREBLU™ DAPI

PUREBLU™ DAPI

PUREBLU™ DAPI

PUREBLU™ DAPI

PUREBLU™ DAPI from Bio-Rad (AbD Serotec - FAB)

Bio-Rad

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PUREBLU™ DAPI from Bio-Rad (AbD Serotec - FAB)

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