DAPI Staining Solution from Miltenyi Biotec

(1) Gene regulatory elements determine efficacy of BCMA-targeted CAR-T cell productsJ Immunother CancerSeptember 18, 2026Viktoria Blumenberg, Kristen B June, Filippo Birocchi, Giulia Escobar, Charlotte Graham, Yueyang Fan, Merle K Phillips, Sangwoo Park, Deshea Harris, Henry Anderson, et al.Flow cytometry Cells were stained 30 min at 4°C, resuspended in 4′,6-diamidino-2-phenylindole (DAPI; Miltenyi #130-111-570, 1:100) for live/dead cell discrimination immediately prior to analysis on a NovoCyte Penteon (Agilent).

(2) Nanostructured Ti-6Al-4V Reduces Adhesion of Several Bacterial Species: An In Vitro StudySmall SciJuly 28, 2026Sadaf Khalatbarizamanpoor, Adrian G. Nowotnick, Stephanie Lippmann, Antje Häder, Felix Otto, Lena Raupach, Torsten Fritz, Yasmina Reisser, Jörg Bossert, Bettina Löffler, et al.concentration) for 15 min at RT in the dark. Nuclei were counterstained using the DAPI Staining Solution (Cat. 130‐111‐570, Miltenyi Biotec, Bergisch Gladbach, Germany; supplied at 10 µg/mL, used at a final concentration of

(3) The transcription factor SREBP2 supports the activation and homeostasis of CD4+ Foxp3+ regulatory T cellsJ ImmunolJuly 17, 2026Acacia N Shouse, Alejandro V Villarino, Thomas R Malek[Miltenyi Biotec; #130-104-454] and LS Columns [Miltenyi Biotec; #130-042-401]). DAPI (Miltenyi Biotec; #130-111-570) was used at a final concentration of 0.1 μg/mL to determine viability. BD FACSAria II system was used

(4) Transcriptomic analysis of tissue-resident memory T cells of the fallopian tube reveals a precursor immune surveillance network for ovarian cancer preventionNature CommunicationsJuly 13, 2026Luyao Wang, Breeshey Roskams-Hieter, Nosheen Hussain, Joel Nulsen, Aneesh Aggarwal, May Sallam, Lili Wang, Lena Rai, Hui Shi, Amro Ahmed-Ebbiary, et al.sorted). FACS gates were drawn to include only live single cells based on DAPI (Miltenyi Biotec, Catalog # 130-111-570). Further gates were drawn to arrive at CD3 + CD103+EpCAM− (for CD103+selected samples) or CD3-EpCAM+

(5) Protocol for minimized-bias profiling of liver and visceral adipose tissue in mice using integrated single-nucleus RNA sequencingSTAR ProtocJuly 8, 2026Lei Li, Jonathan G. Pol, Christophe Desterke, Wanchao Hu, Nicolas Trainel, Morgane Benardeau, Manuela Lizarralde-Guerrero, Maria Chiara Maiuri, Guido Kroemer, Gabriel Perlemuter, et al.ethanol,Major supplier,N/A Nuclease-free water,Major supplier,N/A DAPI Staining Solution,MILTENYI BIOTEC,Cat#130-111-570 "RNase inhibitor, 40000 U/mL",New England Biolabs,Cat#M0314 BD Stain Buffer (FBS),BD Biosciences,Cat#554656 "Proteinase

(6) Protocol for multiplexed detection of antibody binding to cell-surface-expressed hemagglutinin by flow cytometrySTAR ProtocJune 19, 2026Leon Ullrich, Katharina Daniel, Anna-Lena Schumacher, Maximilian Germer, Florian Klein, Christoph Kreerpeptides, and recombinant proteins" ,, "4′,6-Diamidino-2-phenylindole (DAPI)",Miltenyi Biotec,Cat#130-111-570 "Branched Polyethylenimine (PEI), 25 kDa",Sigma-Aldrich,Cat#408727CAS: 9002-98-6 Cytofix™ fixation

(7) Aging disrupts spatiotemporal coordination in the cycling murine ovaryNat AgingJune 17, 2026Tammy C. T. Lan, Alison Kochersberger, Ruth Raichur, Sophia Szady, Hien Tran, Radiana Simeonova, Ashley Helmuth, Andrew Minagar, Maria José Orozco Fuentes, Vipin Kumar, et al.HCR Gold Amplifier Wash Buffer (15 min each), counterstained with DAPI (1 µg ml−1 in PBS; Miltenyi, 130-111-570), washed with PBS and treated with TrueBlack (1× in 70% ethanol; Biotium, 23007) for 1 min at RT to

(8) HLA-DRB1*15:01 drives sex- and age-dependent microglial immune phenotypes and neuroimmune signalingFrontiers in ImmunologyJune 16, 2026Elsa M. Reyes-Reyes, Dhanalakshmi Chinnasamy, Michael D. Trial, Fernando Fernandez, Vincent D. Nguyen, Qianying He, Angel-Grace Leslie, Jessica Begay, Jessica Triplett, David Bradford, et al.Cell pellets were resuspended in PBS for acquisition. For unfixed samples, DAPI (Miltenyi Biotec, #130-111-570) was added immediately prior to data acquisition to exclude dead cells. Samples were analyzed by flow cytometry

(9) Patient induced pluripotent stem cells identify specificities of a reticular pseudodrusen phenotype in age-related macular degenerationGenome MedMay 10, 2026Jenna C. Hall, Kavitha Krishna Sudhakar, Maciej Daniszewski, Anne Senabouth, Carla J. Abbott, Helena H. Liang, Himeesh Kumar, Grace E. Lidgerwood, Mehdi Mirzaei, Jessica Yw Ma, et al.suspension containing RPE cells and bound FITC-POS. RPE cells were incubated with DAPI (0.1 µg/mL, Miltenyi, 130–111–570), passed through a 35 µm cell strainer, then analysed on a Becton Dickinson FACS Aria III flow cytometer.

(10) Receptor-guided AAV Tropism Engineering via MATCHbioRxivApril 1, 2026Nolan Graham, Satheesh Kumar, Joseph Rainaldi, Seoin Yang, Andrew Portell, Billy Santoso, Prashant Malicentrifuged and resuspended in 1× PBS with 2% FBS and 0.1 μg/mL DAPI Staining Solution (Miltenyi Biotec, 130-111-570), pH 7.4 at 1 × 106 cells/mL and analyzed on the BD LSRFortessa™ Cell Analyzer, gating for live cells

(11) Expansion of bone marrow adipocytes in obese mice leads to PD-L1-driven bone marrow immunosuppression and osteoclastogenesisBone ResMarch 20, 2026Samantha N. Costa, Carolyn Chlebek, Lindsey Gray, Peter Caradonna, Sergey Ryzhov, Clifford J. Rosenand non-viable cells were distinguished using DAPI (to detect dead nucleated cells) (Miltenyi Biotec 130-111-570). Statistical analyses All data were graphed and analyzed using GraphPad Prism 10.4.1 software. Data

(12) Loss of Endothelial YAP/TAZ Reduces the Size of Chronic Stroke Lesions and Alters the Endothelial EnvironmentJournal of the American Heart AssociationMarch 17, 2026Ria Göttert, Majed Kikhia, Marie‐Louise Herzog, Wen Pan, Alexandra Klaus‐Bergmann, January Weiner, Dieter Beule, Samuel Knauss, Golo Kronenberg, Michael Potente, et al.BioLegend) were used: SYTOX green (Thermo Fisher Scientific, #S7020, 1:1000), DAPI (Miltenyi Biotec, #130‐111‐570, 1:100), CD45‐APC (#103112, 1:100), CD31‐PE/Cy7 (#102523, 1:100), CD45‐PE (#103106, 1:400), CD11b‐APC

(13) Vascular STING activation facilitates NK cell anti-tumor immunity in small cell lung cancerCancer CellMarch 5, 2026Marco Campisi, Tatsuya Osaki, Ian Dryg, Carla Stornante, Jacqueline Wolff, Jason Weirather, Nicholas Weaver, Mubin Tarannum, Ian Gillanders, Alan Bers, et al.room temperature. Nuclei were counterstained with DAPI staining solution (500:1, Miltenyi Biotec, Cat# 130-111-570) for 15 minutes at RT and mounted using EverBrite™ Hardset Mounting Medium with DAPI (Biotium, Cat#

(14) Mutational Signatures and Clonal Hematopoiesis in Intestinal Metaplasia across Countries with Varying Stomach Cancer IncidenceCancer DiscoveryMarch 2, 2026Kie Kyon Huang, Takeshi Hagihara, Benedict Shi Xiang Lian, Zhi Xuan Ong, Shen Kiat Lim, Roxanne Hui Heng Chong, Supriya Srivastava, Jason Xing Kang, May Yin Lee, Angie Lay-Keng Tan, et al.secondary antibodies at room temperature for 1 hour. Finally, the sections were stained with DAPI (cat. #130-111-570, Miltenyi Biotec) for 1 minute before observation. Mutational Signatures We used SigProfiler (RRID:

(15) Mutational Signatures and Clonal Hematopoiesis in Intestinal Metaplasia across Countries with Varying Stomach Cancer IncidenceCancer DiscovJanuary 14, 2026Kie Kyon Huang, Takeshi Hagihara, Benedict Shi Xiang Lian, Zhi Xuan Ong, Shen Kiat Lim, Roxanne Hui Heng Chong, Supriya Srivastava, Jason Xing Kang, May Yin Lee, Angie Lay-Keng Tan, et al.secondary antibodies at room temperature for 1 hour. Finally, the sections were stained with DAPI (cat. #130-111-570, Miltenyi Biotec) for 1 minute before observation. Mutational Signatures We used SigProfiler (RRID:

(16) Modelling EWS::FLI1 protein fluctuations reveal determinants of tumor plasticity in Ewing sarcomaEMBO Molecular MedicineJanuary 3, 2026Veveeyan Suresh, Christoph Hafemeister, Andri Konstantinou, Sarah Grissenberger, Caterina Sturtzel, Martha M Zylka, Florencia Cidre-Aranaz, Andrea Wenninger-Weinzierl, Karla Queiroz, Dorota Kurek, et al.DPBS). DAPI was added at a final concentration of 10 µg/mL (DAPI Staining Solution Miltenyi Biotec, Cat#130-111-570) to exclude dead cells. The fluorescence intensity of mNG was measured using a BD LSRFortessa Cell Analyzer

(17) CAR T cell engineering impacts antigen-independent activation and co-inhibitionMolecular Therapy Methods & Clinical DevelopmentDecember 11, 2025Christoph Schultheiß, Simon Stücheli, Brenda Besemer, Maja Kadel, Paul Schmidt-Barbo, Andreas Zingg, Natascha Franz, Sarah Adamo, Frank Stenner, Claudia Fischer, et al.half incubated for 24 h. Killing of target cells was assessed by gating for viable (DAPI-negative, #130-111-570, Miltenyi Biotec) CD3−,CD19+,R110+ target NALM-6-R110 cells and by normalizing their percentage after

(18) Loss of Endothelial YAP/TAZ Reduces the Size of Chronic Stroke Lesions and Alters the Endothelial EnvironmentJ Am Heart AssocNovember 26, 2025Ria Göttert, Majed Kikhia, Marie‐Louise Herzog, Wen Pan, Alexandra Klaus‐Bergmann, January Weiner, Dieter Beule, Samuel Knauss, Golo Kronenberg, Michael Potente, et al.BioLegend) were used: SYTOX green (Thermo Fisher Scientific, #S7020, 1:1000), DAPI (Miltenyi Biotec, #130‐111‐570, 1:100), CD45‐APC (#103112, 1:100), CD31‐PE/Cy7 (#102523, 1:100), CD45‐PE (#103106, 1:400), CD11b‐APC

(19) Somatic gene delivery faithfully recapitulates a molecular spectrum of high-risk sarcomasNat CommunJune 16, 2025Roland Imle, Daniel Blösel, Felix K. F. Kommoss, Sara Placke, Eric Stutheit-Zhao, Christina Blume, Dmitry Lupar, Lukas Schmitt, Claudia Winter, Lena Wagner, et al.Buffer (Miltenyi Biotec, 130-121-565) before nuclear staining was performed with DAPI (Miltenyi Biotec, 130-111-570) diluted 1:10 in running buffer. Slides were washed three times with running buffer and submitted to

(20) Single-cell transcriptomic profiling reveals diversity in human iNKT cells across hematologic tissuesCell ReportsMay 27, 2025Reyka G. Jayasinghe, Derek Hollingsworth, Nathan C. Schedler, Emily Landy, Chaiyaporn Boonchalermvichian, Biki Gupta, Hao Yan, Jeanette Baker, Beruh Dejene, Kenneth I. Weinberg, et al.Cryoprotective Medium,Lonza,Catalog # BW12–132A "4’,6-diamidino-2-phenylindole (DAPI)",Miltenyi,Catalog # 130–111-570 ,, Critical commercial assays,, ,, "Anti-iNKT MicroBeads, human",Miltenyi,Catalog # 130–094-842 LS Columns

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DAPI Staining Solution from Miltenyi Biotec

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DAPI Staining Solution from Miltenyi Biotec

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