rCutSmart Buffer from New England Biolabs

Product Specs
ItemrCutSmart Buffer
CompanyNew England Biolabs
Price $27.00 Inquire
Catalog NumberB6004S
Temperature-20 C
ApplicationsDNA Manipulation
TypeBuffer
Quantity5 ml

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) ATP release mediated by TRPM3 enhances invasion in glioblastomaAnimal Cells and SystemsDecember 31, 2026Kkot Garam Bae, Chae Won Song, Jae Hong Yoo, Myunghoon Lee, Noah Koo, Gangsan Lee, Yongmin Mason Park, Jihwan Yoo, In-Young Hwang, Dong Ho Woo, et al.digested using XhoI (BioLabs, #R0146S) and HpaI (BioLabs, #R0105S) with rCutSmart buffer (BioLabs, #B6004S). DNA purification was performed using the Labopass Gel and PCR Clean-up Kit (Cosmo Genetech, #CMA0112).

(2) Miniaturization of CRISPRa plasmids for efficient delivery into renal epithelial cells and Pkd1 transactivationMolecular Biology ReportsDecember 1, 2026Anubhav Chakraborty, Alan S.L. Yuminiaturization, 3 μg of the dCas9-VP64-T2A-EGFP-2XEsp3I plasmid was mixed with 1X rCutSmart Buffer (NEB #B6004S), 800 U of T4 DNA ligase (NEB #M0202S), 40 U of Esp3I (NEB #R0734S), and 2 mM ATP (NEB #P0756S) in a

(3) Protocol for quantification of AAV-CRISPR-mediated hepatic gene editing in miceSTAR ProtocolsDecember 1, 2026Kelsey E. Jarrett, Rodrigo Baltazar-Nunez, Chloe Anne Borja, Precious Juvie Calderon, Rochelle W. Lai, Angela Cheng, Manasvi Paudel, Gabriella E. Rubert, Anthony Suberski, Owen K. Traina, et al.,New England Biolabs,Cat#R0130S PvuII-HF restriction enzyme,New England Biolabs,Cat#R3151S CutSmart Buffer (10×),New England Biolabs,Cat#B6004S T4 DNA Ligase,New England Biolabs,Cat#M0202S T4 DNA Ligase Buffer (10×),New England Biolabs,Cat#B0202S KAPA HiFi

(4) Proteoliposome Preparation and Functional Reconstitution of Human Mitochondrial Transporters Via Wheat Germ Cell-Free Expression SystemCurr ProtocOctober 26, 2026Davood Kharaghani, Haotian Deng, Xiaoyang SuAM9914G; Sigma‐Aldrich, cat. no. W4502‐1010X50ML)10× rCutSmart buffer (New England Biolabs, cat. no. B6004S)EcoRV‐HF (New England Biolabs, cat. no. R3195S)Wizard HMW DNA extraction kit (Promega, cat. no. A9281)Q5 reaction buffer pack

(5) A Rapid Method to Rank Proteins of Widely Varying Stability: Application to Paramyxovirus Foot DomainsCurr ProtocOctober 22, 2026Andrew C. Hausrath, Richard L. Kingston, Megan M. McEvoyStrategic Planning)10× restriction enzyme buffer (e.g., rCutSmart Buffer, New England Biolabs, cat. no. B6004S, supplied with BSER1)H2O, RNase‐freeBseR1 restriction enzyme (e.g., New England Biolabs, cat. no. R0581S)Alkaline

(6) G-quadruplex structures affect the immune response to bacterial infection in mouse macrophagesNat CommunOctober 20, 2026Philipp Schult, Melanie Kastl, Jennifer Szlapa, Konstantinos Symeonidis, Salie Maasewerd, Bernardo Franklin, Alea Radcke, Stefan A. Juranek, Zeinab Abdullah, Katrin Paeschke4 h at 4 °C. To reduce genomic background, beads were washed twice with 1× rCutSmart buffer (NEB, #B6004S), resuspended in 100 µl of the same buffer with 2 U double-strand-specific dsDNase (Sigma Aldrich, #EN771),

(7) Rapid and reversible regulation of cell cycle progression in budding yeast using optogeneticsCell Reports MethodsOctober 19, 2026Andriana Koutsoumpa, Catherine Loukogiannaki, Danny Incarnato, Andreas Milias-Argeitis,New England Biolabs,Cat#M0491S 5× Q5 reaction buffer,New England Biolabs,Cat#B9027S 10× Buffer rCutSmart,New England Biolabs,Cat#B6004S PvuII-HF,New England Biolabs,Cat#R3151S ,, Critical commercial assays,Critical commercial assays,Critical

(8) Protocol for Cas9 nickase-mediated induction and two-dimensional gel detection of i-loops at human telomeric and alpha satellite repeatsSTAR ProtocOctober 6, 2026Elia Zanella, Flavia Bruno, Ylli DoksaniC1016 Chloroform (molecular biology grade),Sigma-Aldrich,Cat# C2432 CutSmart Buffer (10x),NEB,Cat# B6004S "Dimethyl sulfoxide (DMSO), sterile",Sigma-Aldrich,Cat# D2438 "DNase I (RNase-free, lyophilized)",Worthington,Cat#

(9) An FTA card-based CRISPR/Cas12a platform with isothermal amplification for portable tuberculosis diagnosisFrontiers in MicrobiologySeptember 29, 2026Jiayin Xing, Xiangxiang Zhao, Gaojie Wang, Shilong Ren, Lei Chen, Wenbin Diao, Meihua Qu, Zhimin Chang, Xiaoqian Wang, Shihao Zhao, et al.assays. All Cas12a reactions were carried out in CutSmart Buffer (New England Biolabs, USA; Cat. No. B6004S). 2.2 Clinical samples A total of 31 clinical serum samples were included in this study, comprising

(10) A human cell-free translation screen identifies the NT-2 mycotoxin as a ribosomal inhibitor that binds the peptidyl transferase centerCommun BiolSeptember 23, 2026Nino Schwaller, Dominic Andenmatten, Jonas Luginbühl, Julius Rabl, Helena Baur, Marc Chambon, Jonathan Vesin, Gerardo Turcatti, Evangelos D. Karousisreactions containing 40 ng/ml DNA (8 µg in total) in 1x CutSmart buffer (New England Biolabs, Cat No. B6004S) overnight at 37 °C. After confirming linearization by agarose gel electrophoresis, the linearized DNA

(11) Integration of cell-specific gene expression and chromatin accessibility facilitates localization of neurodegenerative risk in microgliabioRxivSeptember 17, 2026Xylena Reed, Dominic J. Acri, Alexandra Beilina, Jinhui Ding, Sara Saez-Atienzar, Mary Kaileh, Sarah Bromberek, Fangle Hu, Grigoriy Lerner, Daniel M. Ramos, et al.07958927001). Following digestion of the plasmid with restriction enzymes SpeI (NEB, R3133 in rCutSmart™ (NEB, B6004S) for 2 hours at 37°C, the digested backbone was gel-purified (Qiagen, 28706; QIAquick Gel Extraction

(12) Targeting the DNA-repair chromatin-modifier protein SMYD3 as a novel epigenetics-based therapy for gastric cancerJ Exp Clin Cancer ResSeptember 12, 2026Katia De Marco, Marialaura Latrofa, Giovanna Forte, Martina Lepore Signorile, Elisabetta Di Nicola, Paola Sanese, Candida Fasano, Vittoria Disciglio, Erica Candela, Sergio Coletta, et al.recombinant shrimp alkaline phosphatase (rSAP; M0371L, New England Biolabs) in 1× rCutSmart Buffer (B6004S, New England Biolabs) at 37 °C for 2 h before immunoblot analysis. Tissues were collected and homogenized

(13) Caloric restriction modulates genome-wide somatic mutation in miceCellSeptember 9, 2026Marta Grońska-Pęski, Victoria Acosta-Rodríguez, Amoolya Srinivasa, Amanda Garrido, Shany Picciotto, Carla B. Green, Joseph S. Takahashi, Jonathan E. Shoag, Gilad D. Evronywere fragmented by adding 5 μL fragmentation mix comprised of 2.5 μL of 10X rCutSmart Buffer (NEB, B6004S), 0.5 μL HpyCH4V (NEB, R0620S), and 2 μL NFW, and then incubating at 37 °C for 15 mins with the lid

(14) Barcoded Rabies In Situ Connectomics for high-throughput reconstruction of neural circuitsNature CommunicationsSeptember 2, 2026Alexander Becalick, Antonin Blot, Molly Strom, Petr Znamenskiy(New England Biolabs, R0176L), 500 ng linear barcoded DNA, 5 μl CutSmart buffer (New England Biolabs, B6004S) and molecular-grade water up to 50 μl. Heat inactivation was then carried out at 80 ∘C for 20 min.

(15) Response of the replisome to araA and araC suggests a mechanism for arabinosyl nucleoside toleranceThe EMBO JournalAugust 27, 2026Harry Orrin, Joseph T P YeelesK,New England Biolabs,P8107S PstI-HF,New England Biolabs,R3140S rCutSmart Buffer,New England Biolabs,B6004S Ribonuclease A (RNaseA),Sigma-Aldrich,R4875 Sepharose 4B gel matrix,Sigma-Aldrich,4B200 Skim Milk

(16) Integrative spatial profiling of 3D genome organization and gene expression in tissueCellAugust 25, 2026Pengfei Guo, Yan Cui, Jincan He, Abraham J. Waldman, Jiaxin Zhu, Yufan Chen, Zhi Huang, Jingtian Zhou, Jennifer E. Phillips-Cremins, Yanxiang Deng,New England Biolabs,B7203S NEBuffer™ r2.1,New England Biolabs,B7202S rCutSmart™ Buffer,New England Biolabs,B6004S DpnII,New England Biolabs,R0543S DdeI,New England Biolabs,R0175S HinfI,New England Biolabs,R0155S DPBS,Thermo Fisher Scientific

(17) A Luciferase-Based Assay for Assessing Cap-Independent Translation in Wheat Germ ExtractBio ProtocAugust 20, 2026Max Cortot, Thorsten Stehlik, Aline Koch, Timo Schlemmer(Thermo Fisher Scientific, catalog number: Q10210) 38. rCutsmartTM buffer (New England Biolabs, catalog number: B6004S) 39. RiboRuler high-range RNA ladder (Thermo Fisher Scientific, catalog number: SM1821) 40. RiboRuler low-range RNA ladder

(18) The DLX/Notch axis is necessary for spatiotemporal regulation of neural cell fateNature CommunicationsAugust 20, 2026Ryan F. Leung, Michael See, Ankita M. George, Patrick L. Barry, Kyle A. France, Natalie Charitakis, Mirana Ramialison, Maree C. Faux, David D. Eisenstat(NEB Cat# R0146) and 10 units of KpnI-HF (NEB Cat# R3142) enzymes, with 1x rCutSmart buffer (NEB Cat# B6004S). Following incubation at 37 °C for 1 h, the products were separated on a 1% (w/v) agarose gel. The

(19) Intrinsic and non-cell-autonomous roles for the neurodevelopmental syndrome-linked transcription factor UNC-3/EBFNature CommunicationsAugust 11, 2026Jayson J. Smith, Seth R. Taylor, Honorine Destain, Grace Kim, David H. Hall, John G. White, David M. Miller, Paschalis Kratsiosdouble-digested at 37 °C for 1 hour with restriction enzymes SmaI and SphI in rCutSmartTM Buffer (NEB Catalog #B6004S). Punc-3_558bp insert was ligated into pKP10_unc-3_cDNA vector with T4 DNA ligase (NEB Catalog #M0202S)

(20) Protocol for optimized methylation context-sensitive enzyme ddRAD library preparation from partially degraded genomic plant DNASTAR ProtocAugust 11, 2026César Herrero-González, Sylvia Sagen Johnsen, Rómulo Sacramento Sobral, Marian Schubert,New England Biolabs,Cat#R0525S T4 DNA ligase,Thermo Fisher Scientific,Cat#EL0011 rCutSmart Buffer,New England Biolabs,Cat#B6004S Agarose E GEN,Labkem/Labbox,Cat#AGAR-E00-500 "DNA Gel Loading Dye, 6×",Thermo Scientific/Thermo Fisher Scientific

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1/9/2023Cloning Reactions with RCutSmart Buffer Scarlette CASSALI use this buffer to prepare DNA fragments for downstream cloning. Then I perform an agarose gel electrophoresis migration of the enzyme digestion products. I excise the DNA fragment of interest from ... Read Review