Description
The 293RTV Cell Line is a permanent line established from primary embryonic human kidney transformed with human adenovirus type 5 DNA. The genes encoded by the E1 region of adenovirus (E1a and E1b) are expressed in these cells and participate in transactivation of viral promoters, allowing these cells to produce high levels of protein.
293RTV also stably expresses the SV40 large T antigen, through cloning and multiple rounds of testing for viral yield, 293RTV is specifically selected for high level of retroviral production. It offers several advantages over the regular 293T cells:
• High retroviral yield
• Firm attachment to culture plates and fast growing
• Ideal as a host when making retrovirus by cotransfection
Note: We recommend cotransfection of expression vector:gag-pol vector:envelope vector at the following plasmid ratios:
(a) For ecotropic or amphotropic retrovirus, 3:1:1
(b) For VSVG-pseudotyped retrovirus, 3:1:0.5