Description
The CellSensor SIE-bla HEK 293T cell line contains a beta-lactamase reporter gene under control of the SIE response element stably integrated into HEK 293T cells. The cell line was created through sorting by FACS of cells responsive to stimulation of the SIE pathway with interleukin 6 (IL-6). The cell line was validated for DMSO tolerance, incubation time with stimulant, and substrate loading conditions. The CellSensor SIE-bla HEK 293T cell line responds to agonist treatment as expected from literature and can be adapted for high throughput screening for agonists or antagonists of the SIE pathway with compound libraries. Candidate drugs can also be tested for dose response against this cell line. Academic and non-profit customers, please inquire for special pricing