ADCC Bioassay Effector Cells, Propagation Model from Promega

Product Specs
ItemADCC Bioassay Effector Cells, Propagation Model
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG7102
Quantity1 each

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) B7-H3-mediated cis-inhibition of EGFR by a tumor-selective bispecific antibody enhances anti-tumor efficacy and minimizes toxicitiesNature CommunicationsFebruary 24, 2026Jian Guan, Tiongsun Chia, Bin Li, Tianyu Zhu, Zhengguang Liao, Junjie Deng, Fenggen Fu, Weiwei Wu, Chengtao Liu, Ya Liu, et al.Jurkat T cells engineered to express FcγRIIIa (reporter cells) were obtained from Promega (Catalog No. G7102). 1.5 × 105 reporter cells and 1.5 ×104 tumor cells were co-cultured in 96-well culture plates using

(2) The buried S2 apex of SARS-CoV-2 spike elicits an immunodominant germline-restricted public antibody responsebioRxivFebruary 21, 2026Suncheol Park, Jacob Mischka, Nisreen Okba, Anass Abbad, Meng Yuan, Komal Srivastava, Charles Gleason, Lubbertus C.F. Mulder, Jeffrey Copps, Katrina Saam, et al.antibodies (30–0.06 μg/mL) were added to the plate in duplicates. ADCC Bioassay Effector Cells (Promega, G7102) were added (1.5×105 cells/well) and the plates were incubated for 6 hours. 75ul of Bio-Glo luciferase

(3) ROSE12, a novel anti-CTLA-4 FcγRs binding-enhanced antibody activated by extracellular adenosine triphosphate, shows tumor-selective regulatory T-cell depletion and antitumor efficacy without systemic immune activationJournal for ImmunoTherapy of CancerJanuary 9, 2026Hiroki Hayashi, Kanako Tatsumi, Hitoshi Katada, Yutaka Matsuda, Toshiaki Tsunenari, Masaki Honda, Takayuki Nemoto, Shun Shimizu, Momoko Miura-Okuda, Yuri Ikuta, et al.reporter bioassay (Promega). Engineered FcγRIIIA (V158 or F158)-expressing effector cells (Promega, G7102/G9797) with NFAT-luciferase reporter were co-cultured with Raji-human-CTLA-4 (hCTLA-4) target cells

(4) Development of in vitro and in vivo neutralization assays for New World alphaviruses based on the VSV pseudovirus systemEmerging Microbes & InfectionsDecember 31, 2025Yawen Liu, Chunlong Chen, Binfan Liao, Xiuzhu Geng, Weijin Huang, Jianhui Nie0403). SK-N-SH (ATCC, HTB-11), murine FcγRIII ADCC effector cells were obtained from Promega (USA, Cat. G7102). Phylogenetic analysis The E3, E2, and E1 protein sequences of CHIKV (QKY67868.1), GETV (ABK32032.1),

(5) Protective effect and molecular mechanisms of human non-neutralizing cross-reactive spike antibodies elicited by SARS-CoV-2 mRNA vaccinationCell ReportsNovember 26, 2024Jordan J. Clark, Irene Hoxie, Daniel C. Adelsberg, Iden A. Sapse, Robert Andreata-Santos, Jeremy S. Yong, Fatima Amanat, Johnstone Tcheou, Ariel Raskin, Gagandeep Singh, et al.BioassayCell Propagation Model,Promega,G9871 "ADCC Reporter Bioassay, V Variant, Propagation Model",Promega,G7102 ,, Experimental models: Organisms/strains,Experimental models: Organisms/strains,Experimental models:

(6) A human monoclonal antibody targeting the monomeric N6 neuraminidase confers protection against avian H5N6 influenza virus infectionNature CommunicationsOctober 15, 2024Min Wang, Yuan Gao, Chenguang Shen, Wei Yang, Qi Peng, Jinlong Cheng, Han-Ming Shen, Yang Yang, George Fu Gao, Yi Shi293-TII Expression Medium (Sino Biological, M293TII). ADCC bioeffector cell FcγRIIIa cells (Promega, G7102) were cultured at 37°C with 5% CO2 in RMPI 1640 with 10% FBS. Influenza virus H5N6 virus strain A/Yunan/DQ001/2016

(7) Impact of glycoengineering and anti-drug antibodies on the anti-cancer activity of a plant-made lectin-Fc fusion proteinPlant Biotechnology JournalNovember 1, 2022Matthew Dent, Katarina L. Mayer, Noel Verjan Garcia, Haixun Guo, Hiroyuki Kajiura, Kazuhito Fujiyama, Nobuyuki MatobahFcγRIIIa and firefly luciferase downstream of an NFAT response element were obtained from Promega (G7102; Madison, WI) and cultured according to the kit protocol. All cells were incubated at 37 °C with 5%

(8) Hemagglutinin stalk-binding antibodies enhance effectiveness of neuraminidase inhibitors against influenza via Fc-dependent effector functionsCell Reports MedicineAugust 16, 2022Ali Zhang, Hanu Chaudhari, Yonathan Agung, Michael R. D’Agostino, Jann C. Ang, Yona Tugg, Matthew S. MillerpFastBac Dual Expression Vector,Gibco,Cat#10712024 "ADCC Reporter Bioassay, V Variant (Human)",Promega,Cat#G7102 Mouse FcγRIV ADCC Bioassay,Promega,Cat#M1212 Bio-Glo Luciferase Assay System,Promega,Cat#G7940 Expi293

(9) Cancer immune therapy with PD-1-dependent CD137 co-stimulation provides localized tumour killing without systemic toxicityNature communicationsNovember 4, 2021Qiao Y, Qiu Y, Ding J, Luo N, Wang H, Ling X, Sun J, Wu Z, Wang Y, Liu Y, et al.cells/well; Jurkat-CD137 cells, 2×104 cells/well), ADCC effector cells (Jurkat-FcγRllla-NFAT-Luc cells, G7102, Promega) (1.5×105 cells/well) and antibodies were mixed in assay buffer and incubated at 37 °C and

(10) Single Immunization of a Vaccine Vectored by a Novel Recombinant Vaccinia Virus Affords Effective Protection Against Respiratory Syncytial Virus Infection in Cotton RatsFrontiers in immunologyJanuary 1, 2021Russell MS, Thulasi Raman SN, Gravel C, Zhang W, Pfeifle A, Chen W, Van Domselaar G, Safronetz D, Johnston M, Sauve S, et al.nm. The ADCC activities of the serum Abs were measured with the Promega ADCC Reporter Bioassay (Promega, G7102, Madison, WI, USA) according to the manufacturer’s instructions. It has been shown that the mouse FcγRIV

(11) Human Antibodies Targeting Influenza B Virus Neuraminidase Active Site Are Broadly ProtectiveImmunityOctober 13, 2020Madsen A, Dai YN, McMahon M, Schmitz AJ, Turner JS, Tan J, Lei T, Alsoussi WB, Strohmeier S, Amor M, et al.Expi293F™ cells,Gibco™,Cat#A14527 Madin Darby Canine Kidney (MDCK) cells,IRR,Cat#FR58 Human ADCC bioeffector FcγRIIIa cells,Promega,Cat#G7102 Bacterial and Virus Strains,Bacterial and Virus Strains,Bacterial and Virus Strains Influenza virus

(12) A novel rabbit derived anti-HER2 antibody with pronounced therapeutic effectiveness on HER2-positive breast cancer cells in vitro and in humanized tumor mice (HTM)Journal of translational medicineAugust 15, 2020Wege AK, Kirchhammer N, Kazandjian LV, Prassl S, Brandt M, Piendl G, Ortmann O, Fischer S, Brockhoff G.analyzed using a BioGlo™ Luciferase ADCC Reporter Bioassay (Promega GmbH, Walldorf, Germany, cat. # G7102). The commercially available assay contains engineered Jurkat cells (derived from the clone E6-1; ATCC

(13) Hijacking antibody-induced CTLA-4 lysosomal degradation for safer and more effective cancer immunotherapyCell researchAugust 1, 2019Zhang Y, Du X, Liu M, Tang F, Zhang P, Ai C, Fields JK, Sundberg EJ, Latinovic OS, Devenport M, et al.(Tokai, Japan). ADCC reporter assay ADCC bioassay effector cells in propagation model (Catalog number G7102) with engineered expressions of FcγRIIIA (V158) and an NFAT (nuclear factor of activated T cells)-dependent

(14) Targeting Tissue Factor for Immunotherapy of Triple-Negative Breast Cancer Using a Second-Generation ICONCancer Immunology ResearchJune 1, 2018Zhiwei Hu, Rulong Shen, Amanda Campbell, Elizabeth McMichael, Lianbo Yu, Bhuvaneswari Ramaswamy, Cheryl A. London, Tian Xu, William E. CarsonJurkat-based cell line transfected with CD16 and N-FAT/Luciferase, were purchased from Promega (Cat. No. G7102) in 2014 and were grown in RPMI 1640 complete growth medium supplemented with 100 μg/ml G418, 250 μg/ml

(15) Toxicological and pharmacological assessment of AGEN1884, a novel human IgG1 anti-CTLA-4 antibodyPloS oneJanuary 1, 2018Gombos RB, Gonzalez A, Manrique M, Chand D, Savitsky D, Morin B, Breous-Nystrom E, Dupont C, Ward RA, Mundt C, et al.or FcγRIIAH131 with an NFAT-dependent firefly luciferase reporter gene (Promega; catalogue numbers G7102, CS1324F08 and PLOS ONE | https://doi.org/10.1371/journal.pone.0191926 April 4, 2018 5 / 28 Comparison

(16) Antibody-dependent-cellular-cytotoxicity-inducing antibodies significantly affect the post-exposure treatment of Ebola virus infectionScientific reportsMarch 30, 2017Liu Q, Fan C, Li Q, Zhou S, Huang W, Wang L, Sun C, Wang M, Wu X, Ma J, et al.Fluc. The ADCC activities of the mAbs were measured with the Promega ADCC Reporter Bioassay (Promega, G7102, Madison, WI, USA). Animal experiments The mice used in this study were housed and handled strictly

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