Fig 1: Induction of type I and III IFN and ISGs in human and canine keratinocytes from multiple sources. (A) Human cells lines NHEK (normal pooled neonatal foreskin primary keratinocytes, Lonza), NHEK Adult (normal primary keratinocytes derived from a cutaneous site on an adult donor), hTERT/Ker-CT (hTERT-immortalized keratinocytes), and HEKn (normal human neonatal foreskin primary keratinocytes, Lifeline Cell Technology) were stimulated with Poly(I:C), and mRNA expression of IFNs and ISGs was assessed 12 hours after stimulation. (B) Canine cell lines CPEK (Zenbio, Raleigh, NC), and three primary canine keratinocyte cell lines derived from the skin of normal dogs, KC-2, KC-3, and KC-4, were also stimulated with Poly(I:C) and mRNA expression of IFNs and ISGs were assessed 12 hours after stimulation. Data is shown as fold induction (ΔΔCt) of mRNA expression over unstimulated control (set to 1), and normalized to the reference gene, RPL13A. Results are expressed as the mean of 3 independent experiments, performed in duplicate, ± SEM.