NEB Stable Competent E. coli (High Efficiency) from New England Biolabs

Product Specs
ItemNEB Stable Competent E. coli (High Efficiency)
CompanyNew England Biolabs
Price $352.00 Inquire
Catalog NumberC3040H
Quantity20 x 0.05 ml
ApplicationsDNA Manipulation
Cell Type (standardized)Competent Cells
SpeciesEscherichia
Temperature-80 C
Cell TypeCompetent Cells

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Streamlined synthetic regulatory cassette for efficient and photoreceptor-enriched retinal gene expressionMol Ther Nucleic AcidsSeptember 8, 2026Jae-Eun Kwon, Ye Ji Kim, Seung-Hyun Kim, Ok-Seon Kwon, Hyeon-Ki Jang, Yong-Min Choi, Hyeon-Jin Na, Namshik Han, Yibin Chun, Jung Woo Han, et al.Assembly reactions were incubated at 50°C for 15 min, transformed into competent Escherichia coli (C3040H; New England Biolabs), and screened via colony PCR. Positive clones were confirmed through Sanger sequencing

(2) Therapeutic activity of a hematopoietic stem cell-delivered cell-penetrating frataxin in Friedreich’s ataxia modelsCell Reports MedicineJune 16, 2026Jeffrey Pido-Lopez, Shefta E Moula, Enas Shaban, Konstantinos Stamatiou, Bethan J. Critchley, Thomas E. Whittaker, Stina Svensson, Sara Anjomani-Virmouni, Ester Kalef-Ezra, Lucinda Carr, et al.strains,Bacterial and virus strains ,, NEB® Stable Competent E. coli (High Efficiency),New England Biolabs,C3040H ,, Biological samples,Biological samples,Biological samples ,, Gibco Fetal Bovine Serum,ThermoFisher

(3) ProNotch converts extracellular protease activity into programmable transcriptional outputsbioRxivJune 3, 2026Jeremy C. Tran, Christopher J. Kuffner, Aiden C. Reilly, Quan Le, John T. Ngowere cloned using the recombination-deficient New England Biolabs (NEB) Stable E. coli strain (NEB, C3040H) as the transformation host. Transformed cells were selected on antibiotic-containing agar plates and

(4) An ancient retrotransposon provides species-specific tuning of IL-18 inflammatory signalingbioRxivMay 29, 2026Andrea D. Ordonez, Holly Allen, Lynn Sanford, Atma Ivancevic, Ashley Agyepong, Mia Chaw, James P. Bridges, Tony Schountz, Edward B. ChuongIn-Fusion cloning (Takara Bio; 638947). In-Fusion products were transformed into NEB Stable E. coli (NEB; C3040H) and plated on LB agar containing 100 μg/mL carbenicillin. Individual colonies were selected, plasmid

(5) Protocol for an in vivo CRISPR screen for germinal center B cells in mice using ecotropic retrovirusSTAR ProtocMay 22, 2026Jun P. Hong, Michel C. Nussenzweigstrains,Bacterial and virus strains ,, "NEB Stable Competent E. coli, High Efficiency",New England Biolabs,cat# C3040H ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(6) Hypoxia-responsive interaction between P-TEFb, BHLHE40, and Tim8-Tim13 regulates hypoxic gene transcriptionSci AdvMay 22, 2026Shimaa Hassan AbdelAziz Soliman, Simone De Fabritiis, Marta Iwanaszko, Lawrence Austin Lin, Madhurima Das, Sarah Gold, Grant David Andersen, Ram P. Chakrabarty, Navdeep S. Chandel, Ali Shilatifardconstructs were amplified by transformation into stable competent Escherichia coli (New England Biolabs, no. C3040H), and all insertions were verified by Sanger sequencing (ACGT). Lentiviral particles were produced by

(7) Computationally guided discovery of Ly6e/LY6E-dependent AAV capsid variantsiScienceMay 15, 2026Hiroaki Ono, Shoko Fujino, Genshiro A. Sunagawaal.73,Addgene Plasmid #60229 NEB® Stable Competent E. coli (High Efficiency),New England Biolabs,Cat# C3040H pCap-PF1.0,This paper,N/A pCap-PF1.1,This paper,N/A pCap-PF1.2,This paper,N/A pCap-PF1.3,This paper,N/A pCap-PF1.4,This

(8) CFIm25-Dependent Alternative Polyadenylation in AKT2 mRNA Programs Macrophage PolarizationiScienceMay 15, 2026Srimoyee Mukherjee, Atish Barua, Marzieh Naseri, Claire L. Moorestrains,Bacterial and virus strains ,, NEB® Stable Competent E. coli (High Efficiency),New England Biolabs,Cat# C3040H ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(9) A multiplex, prime editing framework for identifying drug resistance variants at scaleCell GenomicsMay 13, 2026Florence M.C. Abadie, Chase C. Suiter, Nahum T. Smith, Riza M. Daza, Mary C. Rominger, Phoebe Parrish, Troy A. McDiarmid, Jean-Benoît Lalanne, Beth Martin, Diego Calderon, et al.reaction was transformed into 50 μL of stable competent E. coli cells (New England Biolabs, Cat. No. C3040H) using the NEB 5 min transformation protocol. 100 μL of transformed E. coli cells was plated on an LB

(10) Increased Telomere Mobility in Progeria is Restored by Isoprenylcysteine Carboxyl Methyltransferase InhibitionbioRxivMay 4, 2026Gabriella Gagliano, Alex Raterink, Xijie Yang, Martin Bergö, Anna-Karin Gustavsson2 μL of the product was transformed into NEB Stable Competent E. coli (High Efficiency) cells (NEB, C3040H) following the manufacturer’s protocol. The plasmid was miniprepped (QIAGEN, 12123) and checked via

(11) Genome-wide screening reveals producer-cell modifications that improve virus-like particle production and delivery potencyNat CommunApril 24, 2026Diana Ly, Hyewon Jang, Adhiraj Goel, Arnav Singh, Aditya Raguram(Thermo Fisher Scientific; F537L). Mach1 (Thermo Fisher Scientific; C862003) or NEB® Stable (New England Biolabs; C3040H) chemically competent E. coli were used for plasmid propagation. Plasmids were isolated using QIAGEN

(12) Integrated in vitro and multi-cohort cross-omics analysis of HTLV-1-associated lung pathology reveals a RelA-dependent mechanism for monocyte recruitment and differentiationMolecular MedicineApril 23, 2026Clément J. F. Heymann, Mieke Gouwy, Robin Hermans, Jean-Claude Twizere, Tatiane Assone, Jorge Casseb, Isaac Racine, Isabelle Cleynen, Edward L. Murphy, Roberta Bruhn, et al.virus strains,Bacterial and virus strains NEB 10-beta/Stable Competent E.coli,New England Biolabs,C3040H Biological samples,Biological samples,Biological samples Buffy coats (Healthy donors),"Red Cross,

(13) Disrupting pegRNA intramolecular complementarity via PBS and spacer sequence alterations can enhance prime editing efficiencyNucleic Acids ResApril 21, 2026Zsuzsanna Biczók, Sarah L Krausz, Dorottya A Simon, Eszter Tóth, Éva Varga, Tamás Annus, Flóra Huba, Máté Varga, Éva Bakos, Elfrieda Fodor, et al.acquired from Sigma-Aldrich. Q5 High-Fidelity DNA Polymerase (#M0491L), NEB Stable Competent E. coli (# C3040H) were from New England Biolabs Inc. NucleoSpin Gel and PCR Clean-up kit (#740609.250) was purchased

(14) Systematic discovery of pro- and anti-HIV host factors in primary human CD4+ T cellsCellApril 20, 2026Ujjwal Rathore, Eli Dugan, Hunter Thornton, Vigneshwari Easwar Kumar, Rama Dajani, Ryan C. Burdick, Janet M. Young, Zachary Steinhart, Reanna Lao, Krista A. Delviks-Frankenberry, et al.strains "Endura Competent Cells, Lucigen",VWR,Cat# 60242-2 NEB Stable Competent E. coli,New England Biolabs,Cat# C3040H Stbl3 Competent Cells,Berkeley MacroLab,N/A ElectroMax Stbl4 Competent Cells,Fisher Scientific Cells,Cat#

(15) Synaptic proteins that aggregate and degrade slower with aging accumulate in microgliabioRxivApril 12, 2026Ian H. Guldner, Viktoria P. Wagner, Patricia Moran-Losada, Sophia M. Shi, Kelly Chen, Barbara T Meese, Hamilton Oh, Yann Le Le Guen, Nannan Lu, Pui Shuen Wong, et al.by Gibson Assembly. Propagation of AAV plasmids was performed in NEB Stable Competent E. coli (NEB, C3040H) at 30C to avoid mutations in the AAV ITR sequences. AAV Production AAV was custom-produced by VectorBuilder

(16) A synthetic angiotensin II/ACE2-based hormone shunt controlling experimental hypertensionNature CommunicationsApril 11, 2026Gokberk Unal, Maysam Mansouri, Yu-Qing Xie, Christian Mueller, Ghislaine Charpin-El Hamri, Martin FusseneggerCloned vectors were transformed into XL10-Gold ultracompetent cells (New England Biolabs, cat. No. C3040H), subsequently purified using a miniprep kit (Zymo Research, Irvine, California, USA, cat. No. D4054),

(17) NF-κB signaling directs a program of transient amplifications at innate immune response genesbioRxivApril 11, 2026Michael P. Ludwig, Jason R. Wilson, Matthew D. Galbraith, Nirajan Bhandari, Lauren N. Dunn, Joshua C. Black, Kelly D. Sullivanminutes. Afterwards, 2 μL of the assembly reaction mixture was transformed via heat shock into the C3040H competent cells (NEB, cat # C3040H). 200 μL of LB media was added to transformed cells and incubated

(18) Aspartate Transaminases Are Dispensable for Pancreatic Development and Pancreatic Cancer ProgressionbioRxivApril 10, 2026Julia Ugras, Noah Nelson, Samuel A. Kerk, Damien Sutton, Lin Lin, Peter Sajjakulnukit, Teresa Dombrowski, Gillian Davidson, Brooke Lavoie, Dominik Awad, et al.(plasmid #52961)33. Plasmid DNA was expanded by transformation of NEB Stable competent cells (NEB, C3040H) and maxi prepped using the EndoFree Plasmid Maxi Kit (Qiagen, 12362). The cloning vector was prepared

(19) Distinguishing Pseudotransduction and True Transduction Enables Characterization and Bioengineering of Extracellular Vesicle-Adeno-Associated Virus VectorsJ Extracell VesiclesApril 6, 2026Jonathan D. Boucher, Devin M. Stranford, Hailey I. Edelstein, Danielle Tullman-Ercek, Neha P. Kamat, Joshua N. Leonardtransgene vector backbones were transformed into chemically competent NEB Stable Escherichia coli (NEB C3040H), and cells were grown at 30°C. 2.2 Source Vectors for DNA Assembly The AAV transfer vector (pAAV‐GFP

(20) Adeno-associated virus delivery of anti-alpha toxin monoclonal antibodies confers protection against Staphylococcus aureus infectionsPLoS PathogApril 6, 2026Josefien W. Hommes, Madison E. Hughes, Derek Cheung, Björn Petri, Lindsey M. Orthner, Tristan J. van der Linden, Bart W. Bardoel, Simran K. Deo, Deepak T. Patel, Ronald S. Flannagan, et al.cloning, transformation into NEB Stable competent Escherichia coli (E. coli) cells (New England Biolabs; C3040H) was performed and colonies were selectively grown on 50 µg/mL carbenicillin plates. Eight colonies

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