NEB Stable Competent E. coli (High Efficiency) from New England Biolabs

Product Specs
ItemNEB Stable Competent E. coli (High Efficiency)
CompanyNew England Biolabs
Price $352.00 Inquire
Catalog NumberC3040H
Quantity20 x 0.05 ml
ApplicationsDNA Manipulation
Cell Type (standardized)Competent Cells
SpeciesEscherichia
Temperature-80 C
Cell TypeCompetent Cells

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Computationally guided discovery of Ly6e/LY6E-dependent AAV capsid variantsiScienceMay 15, 2026Hiroaki Ono, Shoko Fujino, Genshiro A. Sunagawaal.73,Addgene Plasmid #60229 NEB® Stable Competent E. coli (High Efficiency),New England Biolabs,Cat# C3040H pCap-PF1.0,This paper,N/A pCap-PF1.1,This paper,N/A pCap-PF1.2,This paper,N/A pCap-PF1.3,This paper,N/A pCap-PF1.4,This

(2) Genome-wide screening reveals producer-cell modifications that improve virus-like particle production and delivery potencyNat CommunApril 24, 2026Diana Ly, Hyewon Jang, Adhiraj Goel, Arnav Singh, Aditya Raguram(Thermo Fisher Scientific; F537L). Mach1 (Thermo Fisher Scientific; C862003) or NEB® Stable (New England Biolabs; C3040H) chemically competent E. coli were used for plasmid propagation. Plasmids were isolated using QIAGEN

(3) Disrupting pegRNA intramolecular complementarity via PBS and spacer sequence alterations can enhance prime editing efficiencyNucleic Acids ResApril 21, 2026Zsuzsanna Biczók, Sarah L Krausz, Dorottya A Simon, Eszter Tóth, Éva Varga, Tamás Annus, Flóra Huba, Máté Varga, Éva Bakos, Elfrieda Fodor, et al.acquired from Sigma-Aldrich. Q5 High-Fidelity DNA Polymerase (#M0491L), NEB Stable Competent E. coli (# C3040H) were from New England Biolabs Inc. NucleoSpin Gel and PCR Clean-up kit (#740609.250) was purchased

(4) Systematic discovery of pro- and anti-HIV host factors in primary human CD4+ T cellsCellApril 20, 2026Ujjwal Rathore, Eli Dugan, Hunter Thornton, Vigneshwari Easwar Kumar, Rama Dajani, Ryan C. Burdick, Janet M. Young, Zachary Steinhart, Reanna Lao, Krista A. Delviks-Frankenberry, et al.strains "Endura Competent Cells, Lucigen",VWR,Cat# 60242-2 NEB Stable Competent E. coli,New England Biolabs,Cat# C3040H Stbl3 Competent Cells,Berkeley MacroLab,N/A ElectroMax Stbl4 Competent Cells,Fisher Scientific Cells,Cat#

(5) Adeno-associated virus delivery of anti-alpha toxin monoclonal antibodies confers protection against Staphylococcus aureus infectionsPLoS PathogApril 6, 2026Josefien W. Hommes, Madison E. Hughes, Derek Cheung, Björn Petri, Lindsey M. Orthner, Tristan J. van der Linden, Bart W. Bardoel, Simran K. Deo, Deepak T. Patel, Ronald S. Flannagan, et al.cloning, transformation into NEB Stable competent Escherichia coli (E. coli) cells (New England Biolabs; C3040H) was performed and colonies were selectively grown on 50 µg/mL carbenicillin plates. Eight colonies

(6) Distinguishing Pseudotransduction and True Transduction Enables Characterization and Bioengineering of Extracellular Vesicle-Adeno-Associated Virus VectorsJ Extracell VesiclesApril 6, 2026Jonathan D. Boucher, Devin M. Stranford, Hailey I. Edelstein, Danielle Tullman-Ercek, Neha P. Kamat, Joshua N. Leonardtransgene vector backbones were transformed into chemically competent NEB Stable Escherichia coli (NEB C3040H), and cells were grown at 30°C. 2.2 Source Vectors for DNA Assembly The AAV transfer vector (pAAV‐GFP

(7) G protein Gαq subunits engage targets in the nucleus involved in chromatin remodeling and gene expressionJournal of Biological ChemistryApril 1, 2026Joseph Loomis, Naincy Chandan, Michael Burroughs, Saji Abraham, Rongxi Zhang, Yiyi Cao, Matthew J. Brody, Gregory G. Tall, Alan V. SmrckaSMARCD3-V5-NP. Plasmid DNAs were amplified using NEB Stable Competent E. coli (New England Biolabs, C3040H) grown at 30 °C to avoid DNA recombination difficulties arising from the pLenti6.3-V5/DEST backbone.

(8) MitoPerturb-Seq identifies gene-specific single-cell responses to mitochondrial DNA depletion and heteroplasmyNat Struct Mol BiolApril 1, 2026Stephen P. Burr, Kathryn Auckland, Angelos Glynos, Abhilesh Dhawanjewar, Cameron Ryall, Wei Wei, Antony Hynes-Allen, Malwina Prater, Matylda Sczaniecka-Clift, Julien Prudent, et al.plating or by heat shock in high-efficiency stable competent Escherichia coli (New England Biolabs (NEB), C3040H). Bacterial colonies (~43,000, estimated ~700× per gRNA) were scraped from plates. Plasmid DNA was extracted

(9) Sulfur partitioning from cysteine controls T cell proliferation and effector functionCellMarch 31, 2026Beth Kelly, Minsun Cha, Tatjana Gremelspacher, Jacob L. Martin, Massimo Andreis, Isha Maloo, Gustavo E. Carrizo, Mia Gidley, Michal A. Stanczak, Petya Apostolova, et al.Listeria monocytogenes-expressing OVA (LmOVA),Pearce Lab, Stable competent E. coli (High Efficiency),New England Biolabs,Cat# C3040H ,, Biological samples,, ,, ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides,

(10) 5-Azacytidine incorporation into mRNAs disrupts translation and induces ribosome collisionsbioRxivMarch 31, 2026Alexis B Roberson, James Marks, Ruby Pitts, Bavavarshini Tamilselvam, Brian Grieb, William P Tansey, Sezen Meydan16°C for 30 minutes. The ligation mixture was transformed to NEB Stable Competent E. coli cells (NEB, C3040H). The resulting lentiGuide-Puro-ZNF598 plasmids were isolated from single colonies and their sequences

(11) Complementary constraints in germ and immune cells shape evolution of gene regulation and phenotypebioRxivMarch 31, 2026Kimberly N Griffin, Kira L Marshall, Gabriel A Russell, John Attanasio, Delaney B Farris, Haoming Yu, Eric Fagerberg, Nivedita R Iyer, Rebecca Lee, Kaelyn D Sumigray, et al.M0202S) for 1 hour at room temperature. Plasmid was transformed into NEB stable competent E. coli (C3040H, NEB) according to manufacturer instructions. Bacteria were plated on LB plates with ampicillin (1:100)

(12) A self-complementary recombinant adeno-associated virus vector coding for an anchorless prion protein carrying the G127V mutation extends survival in a rodent prion disease modelbioRxivMarch 30, 2026Thomas Zerbes, Claire Verkuyl, Cunjie Zhang, Sophie Grunnesjoe, Shehab Eid, Hamza Arshad, Wenda Zhao, Zahra Nasser, Teaghan O’Shea, Ari Belotserkovsky, et al.during a 1-hour incubation at 50°C, then transformed into NEB Stable Competent E. coli (catalog number C3040H, New England Biolabs). Cell culture and rAAV vector production HEK293T cells were maintained in DMEM

(13) Novel Small Molecule GLP-1R Agonists Based on 1H-Benzo[d]imidazole-5-Carboxylic Acid ScaffoldMoleculesMarch 29, 2026Elena V. Tolkacheva, Tagir L. Salakhov, Alexandr Yu. Saliev, Natalia D. Lebedeva, Alisa M. Krasnodubets, Eugene Y. Smirnov, Sergey A. Silonov, Konstantin V. Balakin, Vladimir V. Chernyshov, Roman A. IvanovT4 ligase, and the constructs were transformed into NEB Stable Competent E. coli (High Efficiency) (C3040H, New England Biolabs, Ipswich, MA, USA). After colony screening and verification by Sanger sequencing,

(14) Krüppel-like factors 2 and 3 regulate T cell exhaustion by directing T cell residency and migrationImmunityMarch 27, 2026Jian Shen, Ryan J. Brown, Yuqi Zhang, Kexin Gai, Ashley K. Brown, Ashley M. Bauer, Ashley Zhu, Siying Lin, Hongshen Niu, Arjun Kharel, et al.13-1047 Bacterial and Virus Strains,, LCMV Clone 13,Susan Kaech Lab,N/A NEB Stable Competent E. coli,NEB,Cat# C3040H "Chemicals, Peptides, and Recombinant Proteins",, LCMV gp33-41 Peptide (KAVYNFATM),GenScript,Cat#

(15) Macrophages expressing macrophage receptor with collagen structure attenuate liver fibrosis through a tissue restoration phenotypeJCI InsightMarch 23, 2026Sofia Jerez, Shawna A. Cooper, Usman Yaqoob, Maleeha F. Kalaiger, Abid A. Anwar, Mandy Wong, Bushra Arif, Luke C. Doskey, Maria Hernandez-Tejero, William A. Sherman, et al.were injected i.p. 2 days before each BMDM injection. In vivo phagocytosis assay E. coli bacteria (C3040H, New England Biolabs) were transformed using pGLO (1660405, Bio-Rad) plasmid for the expression of GFP

(16) ADA2-deficient cells exhibit increased levels of cell death and metabolic disturbancesCell Death DiscoveryMarch 23, 2026Lisa Ehlers, Marjon Wouters, Bethany Pillay, Selket Delafontaine, Giorgia Bucciol, Marco Baggio, Mariia Dzhus, Anneleen Hombrouck, Alexandra Damerau, Lien De Somer, et al.(#E0554; New England Biolabs) according to the manufacturer’s instructions. Stable competent E. coli (#C3040H, New England Biolabs) were transformed with the generated constructs. Plasmid DNA was purified with

(17) IFNγ-induced antigen loss in chimeric antigen receptor-T cell therapyFront ImmunolMarch 17, 2026Miao Cao, Jasmine Alvarez, Ramkrishna Mitra, Michael Xu, Trevor R. Baybutt, Zhengyang Sun, Oluwatobiloba Taylor, Allison S. Doermann, Ross E. Staudt, Scott A. Waldman, et al.including lentiviral packaging and envelope plasmids, were transformed into NEB Stable Competent E. coli (C3040H, New England Biolabs). E. coli were cultured in LB Broth, Miller (BP1426-2, Fisher Scientific) supplemented

(18) IFNγ-induced antigen loss in chimeric antigen receptor-T cell therapyFrontiers in ImmunologyMarch 17, 2026Miao Cao, Jasmine Alvarez, Ramkrishna Mitra, Michael Xu, Trevor R. Baybutt, Zhengyang Sun, Oluwatobiloba Taylor, Allison S. Doermann, Ross E. Staudt, Scott A. Waldman, et al.including lentiviral packaging and envelope plasmids, were transformed into NEB Stable Competent E. coli (C3040H, New England Biolabs). E. coli were cultured in LB Broth, Miller (BP1426-2, Fisher Scientific) supplemented

(19) CLN3 mediates chloride efflux from lysosomesNeuronMarch 4, 2026Yayu Wang, Kai Li, Wei Chen, Chao Chen, Adeline J.H. Yong, Xiaofan Zhang, Marena Tynan-La Tynan-La Fontaine, Yuh Nung Jan, Lily Yeh Janand virus strains,Bacterial and virus strains NEB® Stable Competent E. coli,New England Biolabs,Cat# C3040H DH5α Competent Cells,This paper,N/A "Chemicals, peptides, and recombinant proteins","Chemicals, peptides,

(20) Complete and partial forms of X-linked MCTS1 deficiency in patients with mycobacterial diseaseJournal of Human ImmunityMarch 2, 2026Qinhua Zhou, Ivan Bagarić, Fabian Komma, Chandhana Prakash, Hassan Abolhassani, Zahra Chavoshzadeh, Lulu Tsao, Taja Vatovec, Camille Soudée, Jérémie Rosain, et al.Takara). The new plasmids were used to transform NEB Stable Competent E. coli (High Efficiency) (Cat# C3040H; New England Biolabs) and were then extracted with the QIAprep Spin Miniprep Kit (Cat#27104; QIAGEN).

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