Irradiated Mouse Embryonic Fibroblasts (5 vials) from R&D Systems, a Bio-Techne Brand

Product Specs
ItemIrradiated Mouse Embryonic Fibroblasts (5 vials)
CompanyR&D Systems, a Bio-Techne Brand
Price $283.00Supplier Page View Company Product Page
Catalog NumberPSC001
Quantity1 Pack
SpeciesMouse
Cell TypeStem Cell
StorageStore in liquid nitrogen for up to 2 years
Cell Type (standardized)Fibroblasts
Tissue (standardized)Embryo

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Characterization of the intraspecies chimeric mouse brain at embryonic day 12.5Cell TransplantationNovember 11, 2025Phoebe Strell, Madison A. Waldron, Sether T. Johnson, Anala Shetty, Andrew T. Crane, Yun You, Clifford J. Steer, Walter C. Lowtransmembrane bound eGFP 40 . The miPSCs were cultured on irradiated mouse embryonic fibroblasts (#PSC001, R&D Systems, Inc., Minneapolis, MN) in miPSC medium composed of knockout DMEM with 4.5 g/l D-glucose

(2) Characterization of the intraspecies chimeric mouse brain at embryonic day 12.5bioRxiv : the preprint server for biologyApril 2, 2025Strell P, Waldron MA, Johnson S, Shetty A, Crane AT, Steer CJ, Low WC.

(3) Early chromosome condensation by XIST builds A-repeat RNA density that facilitates gene silencingCell reportsJuly 25, 2023Valledor M, Byron M, Dumas B, Carone DM, Hall LL, Lawrence JB.fibroblast). iPSCs and ESC were maintained on irradiated mouse embryonic fibroblasts (iMEFs) (R&D Systems, PSC001) in hiPSC medium containing DMEM/F12 supplemented with 20% Knockout Serum Replacement, 1mM glutamine,

(4) Transcriptional metabolic reprogramming implements meiotic fate decision in mouse testicular germ cellsCell reportsJuly 25, 2023Zhang X, Liu Y, Sosa F, Gunewardena S, Crawford PA, Zielen AC, Orwig KE, Wang N.Collected germ cells were transfer on to irradiated mouse embryonic fibroblasts (MEF) (R&D system, Cat# PSC001) as feeder cells for expansion and long-term culture. METHOD DETAILS RNA isolation and qPCR Total

(5) Assessing the Wnt-reactivity of cytonemes of mouse embryonic stem cells using a bioengineering approachSTAR protocolsSeptember 17, 2021Junyent S, Reeves J, Habib SJ.mitotically inactivated MEFs (passage 3) are sourced from commercial vendors (R&D Systems, cat. num. PSC001). During passaging, MEFs will be separated from TSCs by selective adhesion.6.Prepare a layer of mitotically

(6) Transcriptional profiling of mESC-derived tendon and fibrocartilage cell fate switchNature communicationsJuly 9, 2021Kaji DA, Montero AM, Patel R, Huang AH.plated onto irradiated primary mouse embryonic fibroblasts (MEFs, 1 × 105 cells/cm2, R&D Systems Cat #PSC001) for 4–5 days in ES media (DMEM with 1 mM non-essential amino acids, 1 mM sodium pyruvate, 2 mM L-glutamine,

(7) Pluripotency state regulates cytoneme selectivity and self-organization of embryonic stem cellsThe Journal of cell biologyApril 5, 2021Junyent S, Reeves J, Gentleman E, Habib SJ.embryonic fibroblasts (MEFs; Tanaka, 2006; Junyent et al., 2020). Briefly, irradiated MEFs (cat. no. PSC001; R&D Systems) were thawed in a six-well plate at 3 × 105 cells/well in MEF media containing DMEM (Life

(8) In vitro generation of mouse polarized embryo-like structures from embryonic and trophoblast stem cellsNature protocolsJuly 1, 2018Harrison SE, Sozen B, Zernicka-Goetz M.1. 7 MATERIALS REAGENTS • Mitotically inactivated mouse embryonic fibroblasts (MEFs) ready to use (R&D systems, PSC001) or prepared from mouse embryos as described in Box 1. • Mouse trophoblast stem cells (derived as

(9) Translating dosage compensation to trisomy 21NatureAugust 15, 2013Jiang J, Jing Y, Cost GJ, Chiang JC, Kolpa HJ, Cotton AM, Carone DM, Carone BR, Shivak DA, Guschin DY, et al.Hospital Boston, USA) and maintained on irradiated mouse embryonic fibroblasts (iMEFs) (R & D Systems, PSC001) in hiPSC medium containing DNEM/F12 supplemented with 20% knockout Serum Replacement (Invitrogen),

(10) A comparative study of protocols for mouse embryonic stem cell culturingPloS oneJanuary 1, 2013Tamm C, Pijuan Galitó S, Annerén C.

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