C57BL/6 Mouse Primary Brain Microvascular Endothelial Cells - Frozen Vial from Cell Biologics

Product Specs
ItemC57BL/6 Mouse Primary Brain Microvascular Endothelial Cells - Frozen Vial
CompanyCell Biologics
Price $590.00Get Pricing Get Quote for this Product
Catalog NumberC57-6023
QuantityFrozen Vial
SpeciesC57BL/6, Mouse
Cell Type (standardized)Microvascular Endothelial Cells
Tissue (standardized)Brain
StorageLiquid Nitrogen
Cell TypePrimary Cell
TissueBrain
Concentration0.5 × 10^6 cells/mL
TypeAdherent

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Cell Biologics

2201 W Campbell Park Dr Suite 326

Chicago, Illinois 60612

United States

Phone: (312) 226-8198
(312) 981-9263

Email: info@cellbiologics.com

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Website: cellbiologics.com

(1) Single-cell transcriptomic analyses reveal angiogenic vascular responses to chronic cerebral hypoperfusioniScienceApril 17, 2026Jiajing Shan, Ruyu Shi, Liyuan Jiang, Connor Dufort, Wanying Miao, Ting-Wei Mai, Junxuan Lyu, Julieta Barreiro, Kong Chen, Rehana K. Leak, et al.Cell cultures Primary mouse brain microvascular endothelial cells (mBMECs) were purchased from Cell Biologics (Cat# C57-6023, Cell Biologics, Chicago, IL). These cells were cultured between 2 and 9 passages as recommended on

(2) Dual role of Ninjurin-1 in myeloid cell adhesion and inflammation in relapse-remitting EAEFront ImmunolApril 15, 2026Coleen Thompson, Alex Annett, Anastasia Alkhimovitch, Kashish Singh Parihar, Igal Iferganx 106 cells/well in 24 well plates. C57BL/6 brain microvascular endothelial cells (Cell Biologics, C57-6023) were cultured to 90% confluency and then plated at 30,000 cells/well in a 24 well plate. Cells were

(3) Molecular signature and functional properties of human pluripotent stem cell-derived brain pericytesbioRxivFebruary 27, 2026Ruslan Rust, Abhay P. Sagare, Kassandra Kisler, Youbin Kim, Mingzi Zhang, Casey Griffin, Yaoming Wang, Veronica Clementel, Arkadi Shwartz, Scott E Fraser, et al.In vitro BBB assay. Primary mouse brain endothelial cells (mBEC) were purchased from Cell Biologics, C57–6023, primary human brain endothelial cells (hBEC) were used from brain autopsies of young neurologically

(4) Th17 effector cytokines induce shared and distinct microglial and endothelial cell responses in post-streptococcal encephalitisbioRxivFebruary 7, 2026Charlotte R. Wayne, Ugur Akcan, Travis E. Faust, Violetta Duran-Laforet, Danny Jamoul, Luca Bremner, Nicole Ampatey, Büşra T. Akcan, Sarah J. Ho, Bogoljub Ciric, et al.<1% of the population. Cell culture Primary mouse brain endothelial cells (mBECs; Cell Biologics, C57-6023) and primary human microvascular endothelial cells (HBMEC; Cell System, ACBRI 376) were cultured as

(5) Dual Role of Ninjurin-1 in Myeloid Cell Adhesion and Inflammation in Relapse-Remitting EAEbioRxivJanuary 30, 2026Coleen Thompson, Alex Annett, Anastasia Alkhimovitch, Kashish Singh Singh Parihar, Igal Ifergan× 106 cells/well in 24 well plates. C57BL/6 brain microvascular endothelial cells (Cell Biologics, C57–6023) were cultured to 90% confluency and then plated at 30,000 cells/well in a 24 well plate. Cells were

(6) Trametinib decreased intracerebral hemorrhages and endothelial-to-mesenchymal transition in KRASG12V-induced brain arteriovenous malformations in miceStrokeDecember 18, 2025Ohnmar Myint, Bridger Freeman, Jae Yeong Jeong, Hyejin Park, Samantha M. Wilfur, Shuning Huang, Jung-Eun Park, Jakob Körbelin, Jaroslaw Aronowski, Eun S. Park, et al.MD). Plasmid transfection in primary mouse brain ECs and Trametinib treatment Mouse brain microvascular ECs (C57–6023, Cell Biologics, Chicago, IL, USA) were cultured in DMEM (CM002–050, GenDEPOT, Katy, TX) supplemented

(7) Trametinib Decreased Intracerebral Hemorrhages and Endothelial-to-Mesenchymal Transition in KRAS G12V -Induced Brain Arteriovenous Malformations in MiceStrokeDecember 18, 2025Ohnmar Myint, Bridger H Freeman, Jae Yeong Jeong, Hyejin Park, Samantha M. Wilfur, Shuning Huang, Jung-Eun Park, Jakob Körbelin, Jaroslaw Aronowski, Eun S. Park, et al.MD). Plasmid transfection in primary mouse brain ECs and Trametinib treatment Mouse brain microvascular ECs (C57–6023, Cell Biologics, Chicago, IL, USA) were cultured in DMEM (CM002–050, GenDEPOT, Katy, TX) supplemented

(8) Neutrophil-secreted extracellular vesicles induce blood-brain barrier leakage and tight junction disruptionFluids and Barriers of the CNSDecember 6, 2025Luis Arteaga-Blanco, Maliheh Najari Beidokhti, Nuria Villalba, Lindsay Swaby, Byeong J. Cha, Cemre Kayisli, Yao Yao, Mack H. Wu, Sarah Y. Yuanbrain microvascular endothelial cells (BMECs) from C57Bl/6J mice were purchased from Cell Biologics (#C57-6023) and cultured in the recommended endothelial cell medium (Cell Biologics; #M1168) supplemented with

(9) Rab7a is required to degrade select blood-brain barrier junctional proteins after ischemic strokeActa Neuropathologica CommunicationsSeptember 29, 2025Azzurra Cottarelli, Danny Jamoul, Mary Claire Tuohy, Sanjid Shahriar, Michael Glendinning, Grace Prochilo, Aimee L. Edinger, Ahmet Arac, Dritan Agalliu,Experimental Models,Experimental Models C57BL/6 mouse primary brain microvascular endothelial cells,Cell Biologics,Cat #C57-6023 Rab7a flox/flox,Dr. Aimee Edinger,RRID: IMSR_JAX:021589 Cdh5(PAC)-Cre ERT2 (VEC-PAC),,RRID: IMSR_GPT:

(10) Genetic deletion of microRNA-15a/16-1 in pericytes stimulates cerebral angiogenesis and promotes functional recovery after ischemic strokeAngiogenesisJune 17, 2025Ping Sun, Yang Xu, Tianqing Xiong, Shun Li, Na Qiu, Chao Zhou, Jiefei Wang, Alexander Chang, Uma R. Chandran, Ke-Jie Yinmicrovascular endothelial cells (mBMECs) were purchased from Cell Biologics, Inc. (Chicago, IL, USA, Cat #: C57-6023). Primary mBMECs (2-9 passages) were cultured as recommended on tissue culture-treated plates in complete

(11) Immune cell infiltration into brain tumor microenvironment is mediated by Rab27-regulated vascular wall integritySci AdvMay 23, 2025Lata Adnani, Brian Meehan, Minjun Kim, Dongsic Choi, Christopher E. Rudd, Yasser Riazalhosseini, Janusz Rak1X penicillin/streptomycin (PenStrep) (Life Technologies). Mouse primary brain microvascular ECs (#C57-6023, Cell Biologics Inc.) were cultured in cell culture dishes (#150350, Thermo Fisher Scientific) coated

(12) Interplay between Netrin-1 and Norrin controls arteriovenous zonation of blood–retina barrier integrityProceedings of the National Academy of SciencesDecember 24, 2024Jessica Furtado, Luiz Henrique Geraldo, Felipe Saceanu Leser, Bartlomiej Bartkowiak, Mathilde Poulet, Hyojin Park, Mark Robinson, Laurence Pibouin-Fragner, Anne Eichmann, Kevin BoyéMicrovascular Endothelial Cells (C57-6065) and C57BL/6 Mouse Primary Brain Microvascular Endothelial Cells (C57-6023) were purchased from Cell Biologics. HUVECs were from Lonza (C2519A). Cells were cultured in complete

(13) IL-33/ST2 signaling in monocyte-derived macrophages maintains blood-brain barrier integrity and restricts infarctions early after ischemic strokeJournal of neuroinflammationOctober 24, 2024Wang M, Dufort C, Du Z, Shi R, Xu F, Huang Z, Sigler AR, Leak RK, Hu X.deprivation Primary mouse brain microvascular endothelial cells (BMECs) were purchased from Cell Biologics (C57-6023, Cell Biologics, Chicago, IL, USA). BMECs were grown in mouse endothelial cell medium (Cell Biologics,

(14) 12/15-Lipooxygenase Inhibition Reduces Microvessel Constriction and Microthrombi After Subarachnoid Hemorrhage in MiceTranslational Stroke ResearchSeptember 19, 2024Ari Dienel, Sung Ha Hong, Hussein A. Zeineddine, Sithara Thomas, Shafeeque C. M., Dania A. Jose, Kiara Torres, Jose Guzman, Andrew Dunn, P. Kumar T., et al.brain microvascular endothelial cells from human (HBMVEC, ACBRI-376, Cell Systems) and mouse (MBMVEC, C57-6023, Cell Biologics) and human brain vascular pericytes (ACBRI-498, Cell Systems). HBMVEC and MBMVEC cells

(15) Vascular heterogeneity of tight junction Claudins guides organotropic metastasisNature CancerSeptember 17, 2024Xunian Zhou, Valerie S. LeBleu, Eliot Fletcher-Sananikone, Jiha Kim, Jianli Dai, Bingrui Li, Chia-Chin Wu, Hikaru Sugimoto, Toru Miyake, Lisa M. Becker, et al.Biologics, C57–6011) and C57BL/6 mouse primary brain microvascular endothelial cells (Cell Biologics, C57–6023) were maintained in complete mouse endothelial cell medium (Cell Biologics, M1168) on gelatin (Cell

(16) Physiological platelet aggregation assay to mitigate drug-induced thrombocytopenia using a microphysiological systemScientific ReportsJune 19, 2024Kosuke Harada, Wang Wenlong, Tadahiro ShinozawaECM gel inlet. Then, 50,000–100,000 cells/mL murine brain microvascular endothelial cell suspensions (C57-6023; Cell Biologics, Inc, Chicago, IL, USA) were prepared. Cell suspension (2 µL) was seeded in the medium

(17) 5-HT(1F) receptor agonism induces mitochondrial biogenesis and increases cellular function in brain microvascular endothelial cellsFrontiers in cellular neuroscienceJanuary 1, 2024Scholpa NE, Simmons EC, Thompson AD, Carroll SS, Schnellmann RG.reagents Primary C57bl/6 mouse cerebral endothelial cells (mBMEC) were purchased from Cell Biologics Inc. (C57-6023; Chicago, IL). mBMEC were cultured on 0.1% gelatin-coated flasks using endothelial cell medium (M1168,

(18) Hyperinsulinemia Impairs Clathrin-Mediated Endocytosis of the Insulin Receptor and Activation of Endothelial Nitric Oxide Synthase in Brain Endothelial CellsInternational Journal of Molecular SciencesSeptember 28, 2023Stephanie G. DiLucia, B. Jacob Kendrick, Catrina Sims-RobinsonMicrovascular Endothelial Cell Culture Primary C57BL/6 mouse brain microvascular endothelial cells (MBECs; C57-6023, Cell Biologics, Chicago, IL, USA) were cultured as per our previously established protocol [29]. In

(19) Rab7a activation promotes degradation of select tight junction proteins at the blood-brain barrier after ischemic strokebioRxiv : the preprint server for biologyAugust 30, 2023Cottarelli A, Shahriar S, Arac A, Glendinning M, Tuohy MC, Prochilo G, Neal JB, Edinger AL, Agalliu D.,Experimental Models,Experimental Models C57BL/6 mouse primary brain microvascular endothelial cells,Cell Biologics,Cat #C57-6023 Rab7a flox/flox,Dr. Aimee Edinger,RRID: IMSR_JAX:021589 Cdh5(PAC)-CreERT2 (VEC-PAC),Gift from Dr.

(20) Netrin-1 binding to Unc5B regulates Blood-Retina Barrier integritybioRxivApril 19, 2023Jessica Furtado, Luiz Henrique Geraldo, Felipe Saceanu Leser, Mathilde Poulet, Hyojin Park, Laurence Pibouin-Fragner, Anne Eichmann, Kevin BoyéCell culture C57BL/6 Mouse Primary Brain Microvascular Endothelial Cells were purchased from Cell Biologics (C57-6023). Cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) high glucose (Thermo Fisher Scientific,

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C57BL/6 Mouse Primary Brain Microvascular Endothelial Cells - Frozen Vial from Cell Biologics

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C57BL/6 Mouse Primary Brain Microvascular Endothelial Cells - Frozen Vial from Cell Biologics

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