Fig 1: High productivity of GCSF-MSC generated with polymer based method. MSC were transfected with 200 ng GCSF DNA or 500 ng mRNA complexed with Turbofect or Lipofectamine using centrifugation method or manufacturer's instruction, respectively. Following centrifugation, the transfection mixture was replaced with media with or without DOPE/CHEMS plus 10 μM Tubastatin A. (A) One day later, the cells were lysed for immunoblotting analysis. (B) The GCSF level in the conditioned media was determined with Elisa analysis. Significant differences in GCSF level (ng/ml) were calculated using the two tailed Student's t-test. *P < 0.05. (C) To access the bioactivity of GCSF, the conditioned media was collected and diluted by 100 times in NSF60 culture (in serum free RPMI). After 3 day stimulation, the number of NSF60 was counted using automated cell counter (chemometec). Data presented as mean ± SD of biological triplicates. Significant differences in conditioned media from the transfected and non transfected cultures were calculated using the two tailed Student's t-test. *P < 0.05; **P < 0.005.