
Complementary DNA, or cDNA, specifically refers to a segment of double-stranded DNA produced from gene-coding RNA. They are typically synthesized from the in-vitro reaction of reverse transcriptase with a mature mRNA template. They can then be cloned and stored in plasmids or artificial chromosomes individually or as a collection of different genes in a cDNA library. Representing protein-coding sequences, eukaryotic cDNA can be cloned into bacteria and yeast or transfected into other eukaryotic cells for studying gene expression or protein structure and function. Here, we have organized a number of gene-specific, tissue-specific, and species-specific cDNA and cDNA libraries from a variety of suppliers. Gene-specific cDNA are typically cloned in expression plasmids, and may be provided as a purified plasmid or as bacterial stock. Tissue-specific cDNA are reverse transcription products of total RNA extracted from human and animal tissues. In our cDNA search tool, check desired selections in our provided filters or type in the search box to help narrow down your search.
New methods are increasingly being used to provide a clearer understanding of when and how gene expression is regulated.
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Multipart processes bring a lot of challenges that must be overcome
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Speed and enhanced PCR optimization functions like the 2D-Gradient make the Mastercycler X50 the ideal PCR cycler for advanced research in molecular biology. The excellent block temperature regulation gives rise to the next stage of PCR ...
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The Sapphire FL Biomolecular Imager is a field-upgradable laser scanner, with a wide selection of user-adjustable lasers and filters. It features 5-micron resolution, adjustable Z-plane focus and a wide field of view of 25cm X 25cm. Applications ...
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I have used Bioline's Tetro cDNA synthesis kit for synthesis of cDNA using random hexamers as primer with E. coli RNA samples to measure gene expression by quantitative real time PCR. The kit was very specific and has high sensitivity in synthesis of...
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This is a versatile kit for immediate gene expression analysis, results are obtained within the hour.
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