Fig 1: Overexpression of Ephrin B ligands in normal prostate fibroblasts induce the activation of cancer associated fibroblasts (CAF) markers. (a) Expression of EFNB1, EFNB2, and EFNB3 ligand proteins in lentivirus transduced BHPrS1 cells was validated by Western blot. The bands were quantified and normalized to β-actin and presented as fold change in protein expression compared to BHPrS1EV (n = 3 independent experiments). (b) The protein levels of Vimentin and putative CAF markers, Tenascin-C (TN-C), and alpha-smooth muscle actin (α-SMA) were evaluated in Ephrin-generated cell lines (BHPrS1EFNB1, BHPrS1EFNB2, BHPrS1EFNB3) by Western blot. The bands were quantified and normalized to β-actin and presented as the mean (* p < 0.05, one-way ANOVA, n = 3 independent experiments).