Fig 1: The overexpressed (OE) Dvl1/DVL3 DEP chimera construct activates the WNT/β-catenin pathway more effectively than both OE Dvl1 and Dvl3 alone, as demonstrated by the dual luciferase assay measuring the WNT/β-catenin pathway activity using the Wnt3a conditioned medium (CM) in HEK293 wt and DVL1-2-3 knock-out (KO) cells. For WB lysates and protein levels, see Supplementary Figs. 7 and 9.
Fig 2: Immunofluorescence of HEK293 cells shows that the DVL3/Dvl1 DEP chimera construct binds strongly to the cell membrane in the presence of FZD5-V5 (highlighted by a white arrow), unlike the mDvl1 DEP domain-deleted construct, confirming thus its functionality. Without FZDs, both constructs form puncta. All channels are shown as Supplementary Fig. 5. Scale bar is 10 µm.
Supplier Page from DNASU for DVL3 (Homo sapiens) in pDONR221 (Gateway donor/master vector)