Fig 1: Lack of inhibitory effect of PHL-B on TRPV1, TRPV4, and TRPM8 channels. (A, C, E) Concentration-dependent relationships of PHL-B on TRPV1, TRPV4, and TRPM8 channels upon stimulation with capsaicin, HTS, and menthol, respectively. Capsazepine, HC-067047, and sesamin were used as reference inhibitors for TRPV1, TRPV4, and TRPM8 channels, respectively. (B, D, F) Plots of normalized fluorescence vs drug concentration at different time points for each channel, where the lack of effect of PHL-B can be clearly visualized. These data could not be fitted with a Hill equation; hence, the IC50 values were not determined. Data are expressed as means ± SEM, N = 3–5 independent experiments.