Fig 1: Immunofluorescence of HEK293 cells shows that the DVL3/Dvl1 DEP chimera construct binds strongly to the cell membrane in the presence of FZD5-V5 (highlighted by a white arrow), unlike the mDvl1 DEP domain-deleted construct, confirming thus its functionality. Without FZDs, both constructs form puncta. All channels are shown as Supplementary Fig. 5. Scale bar is 10 µm.
Fig 2: The overexpressed (OE) Dvl1/DVL3 DEP chimera construct activates the WNT/β-catenin pathway more effectively than both OE Dvl1 and Dvl3 alone, as demonstrated by the dual luciferase assay measuring the WNT/β-catenin pathway activity using the Wnt3a conditioned medium (CM) in HEK293 wt and DVL1-2-3 knock-out (KO) cells. For WB lysates and protein levels, see Supplementary Figs. 7 and 9.
Supplier Page from DNASU for DVL3 (Homo sapiens) in pDONR221 (Gateway donor/master vector)