ProtoScript II Reverse Transcriptase M0368S from New England Biolabs

Product Specs
ItemProtoScript II Reverse Transcriptase
CompanyNew England Biolabs
Price $99.00 Inquire
Catalog NumberM0368S
Size4000 units
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(61) Molecular consequences of acute versus chronic CDK12 loss in prostate carcinoma nominates distinct therapeutic strategiesbioRxiv : the preprint server for biologyJuly 19, 2024Frank S, Persse T, Coleman I, Bankhead A 3rd, Li D, De-Sarkar N, Wilson D, Rudoy D, Vashisth M, Galipeau P, et al.and quantified by Qubit (Thermo Q10211). Reverse transcription was performed with ProtoScript II (NEB M0368) using 4μM poly-dT plus 1μM random hexamer primers. qPCR was performed in 384-well plates (15μL reactions)

(62) Translational regulation enhances distinction of cell types in the nervous systemElifeJuly 16, 2024Toshiharu Ichinose, Shu Kondo, Mai Kanno, Yuichi Shichino, Mari Mito, Shintaro Iwasaki, Hiromu TanimotoThermo Fisher Scientific). The rRNA-depleted samples were reverse-transcribed with ProtoScript II (M0368L, New England Biolabs), circularized with CircLigase II (CL9025K, Epicentre), and PCR-amplified using

(63) Translational regulation enhances distinction of cell types in the nervous systemeLifeJuly 16, 2024Toshiharu Ichinose, Shu Kondo, Mai Kanno, Yuichi Shichino, Mari Mito, Shintaro Iwasaki, Hiromu TanimotoThermo Fisher Scientific). The rRNA-depleted samples were reverse-transcribed with ProtoScript II (M0368L, New England Biolabs), circularized with CircLigase II (CL9025K, Epicentre), and PCR-amplified using

(64) Human promoter directionality is determined by transcriptional initiation and the opposing activities of INTS11 and CDK9ElifeJuly 8, 2024Joshua D Eaton, Jessica Board, Lee Davidson, Chris Estell, Steven Westfor Illumina,NEB,Cat# E7765, Commercial assay or kit,Protoscript II reverse transcriptase,NEB,Cat# M0368, Commercial assay or kit,LUNA qPCR reagent,NEB,Cat# M3003, Commercial assay or kit,Ampure XP beads,Beckman Coulter

(65) Human promoter directionality is determined by transcriptional initiation and the opposing activities of INTS11 and CDK9eLifeJuly 8, 2024Joshua D Eaton, Jessica Board, Lee Davidson, Chris Estell, Steven Westfor Illumina,NEB,Cat# E7765, Commercial assay or kit,Protoscript II reverse transcriptase,NEB,Cat# M0368, Commercial assay or kit,LUNA qPCR reagent,NEB,Cat# M3003, Commercial assay or kit,Ampure XP beads,Beckman Coulter

(66) Sse1, Hsp110 chaperone of yeast, controls the cellular fate during endoplasmic reticulum stressG3: Genes, Genomes, GeneticsJune 5, 2024Mainak Pratim Jha, Vignesh Kumar, Asmita Ghosh, Koyeli Mapa(NEB Cat No. B0368S), 2 µl of 0.1 M DTT (NEB Cat No. B1034A), 1 µl of ProtoScipt II RT (NEB Cat No. M0368S), 0.2 µl of RNase inhibitor (Roche Diagnostics Gmbh Cat No. 03335399001), and 2.8 µl of nuclease-free

(67) Loss of tumor-derived SMAD4 enhances primary tumor growth but not metastasis following BMP4 signallingCell Communication and SignalingApril 30, 2024Lap Hing Chi, Andrew D. Redfern, Suraya Roslan, Ian P. Street, Allan D. Burrows, Robin L. AndersonSingle strand cDNA was synthesized using the ProtoScript II reverse transcriptase (New England Biolabs #M0368) with random pentadecamers. Gene expression was determined using the SYBR green real-time PCR master

(68) Transcriptome-wide analysis of the function of Ded1 in translation preinitiation complex assembly in a reconstituted in vitro systemeLifeApril 4, 2024Fujun Zhou, Julie M Bocetti, Meizhen Hou, Daoming Qin, Alan G Hinnebusch, Jon R Lorschpurified for reverse transcription using RT primer NI-802 and ProtoScript II Reverse Transcriptase (NEB; M0368S). The ~105 nt cDNAs were separated from free RT primers on a 15% TBE-Urea gel and circularized using

(69) Transcriptome-wide analysis of the function of Ded1 in translation preinitiation complex assembly in a reconstituted in vitro systemElifeApril 4, 2024Fujun Zhou, Julie M Bocetti, Meizhen Hou, Daoming Qin, Alan G Hinnebusch, Jon R Lorschpurified for reverse transcription using RT primer NI-802 and ProtoScript II Reverse Transcriptase (NEB; M0368S). The ~105 nt cDNAs were separated from free RT primers on a 15% TBE-Urea gel and circularized using

(70) The CARD8 inflammasome dictates HIV/SIV pathogenesis and disease progressionCellFebruary 29, 2024Wang Q, Clark KM, Tiwari R, Raju N, Tharp GK, Rogers J, Harris RA, Raveendran M, Bosinger SE, Burdo TH, et al.,Thermo Fisher Scientific,K1032 Recombinant Cas9 protein,IDT,1081059 ProtoScript II Reverse Transcriptase,NEB,M0368 IPTG,Sigma,I6758 Lenti-X Concentrator,TaKaRa,631232 Critical commercial assays,Critical commercial

(71) Protein-protein interactions with G3BPs drive stress granule condensation and gene expression changes under cellular stressbioRxivFebruary 10, 2024José M. Liboy-Lugo, Carla A. Espinoza, Jessica Sheu-Gruttadauria, Jesslyn E. Park, Albert Xu, Ziad Jowhar, Angela L. Gao, José A. Carmona-Negrón, Torsten Wittmann, Natalia Jura, et al.T4 RNA ligase 2 truncated KQ (NEB, M0373L). Linker-ligated RNA was reverse transcribed with Protoscript II (NEB, M0368L) for 30 minutes at 50°C. Template RNA was hydrolyzed with 1M NaOH for 20 minutes at 70°C. Gel slices

(72) Defining the mechanisms and properties of post-transcriptional regulatory disordered regions by high-throughput functional profilingbioRxivFebruary 8, 2024Joseph H. Lobel, Nicholas T. Ingoliaspectrophotometry. Reverse transcription was performed with dT priming using the ProtoScript II RT (NEB M0368L) as described by the manufacturer, scaled up two-fold. RNA was the digested by adding 0.5 μL each of

(73) Massive contractions of myotonic dystrophy type 2-associated CCTG tetranucleotide repeats occur via double-strand break repair with distinct requirements for DNA helicasesG3: Genes, Genomes, GeneticsFebruary 7, 2024David Papp, Luis A Hernandez, Theresa A Mai, Terrance J Haanen, Meghan A O’Donnell, Ariel T Duran, Sophia M Hernandez, Jenni E Narvanto, Berenice Arguello, Marvin O Onwukwe, et al.(cDNA) was synthesized using 0.5-μg total RNA as template, ProtoScript II Reverse Transcriptase (NEB M0368), and equal amounts of random hexamer primers (Thermo Fisher SO142) and oligo(dT) primers (Thermo Fisher

(74) Hfq protein and GcvB small RNA tailoring of oppA target mRNA to levels allowing translation activation by MicF small RNA in Escherichia coliRNA BiologyDecember 31, 2023Marie-Claude Carrier, David Lalaouna, Eric Massé(R3101), BamHI (R3136), SphI (R3182), MscI (R0534), T4 Polynucleotide kinase (M0201), Protoscript II (M0368), calf intestinal phosphatase (M0290), Q5 High fidelity DNA polymerase (M0491), XRN-1 (M0338) and Vent (exo–) DNA Polymerase

(75) SIKs Regulate HDAC7 Stabilization and Cytokine Recall in Late-Stage T Cell Effector DifferentiationJournal of immunology (Baltimore, Md. : 1950)December 15, 2023Helms RS, Marin-Gonzalez A, Patel CH, Sun IH, Wen J, Leone RD, Duvall B, Gao RD, Ha T, Tsukamoto T, et al.equivalent amounts of cDNA were generated per condition using ProtoScript II Reverse Transcriptase (NEB Cat# M0368L) and random hexamers (Qiagen Cat# 79236) according to manufacturer’s protocol. qRT-PCR for Ifng (ThermoFisher Scientific

(76) A rapid, multiplex digital PCR assay to detect gene variants and fusions in non‐small cell lung cancerMolecular OncologyNovember 1, 2023Bryan Leatham, Katie McNall, Hari K. K. Subramanian, Lucien Jacky, John Alvarado, Dominic Yurk, Mimi Wang, Donald C. Green, Gregory J. Tsongalis, Aditya Rajagopal, et al.Thermo Fisher Scientific), 2.4 μL 5× primer–probe mix (Table S5), 0.6 μL reverse transcriptase (PN M0368S; New England Biolabs, Ipswich, MA, USA), 5 μL RNA sample (1–3 ng total RNA), and 1.6 μL 1× TE Buffer

(77) Fluoroquinolone and beta-lactam antimicrobials induce different transcriptome profiles in Salmonella enterica persister cellsScientific ReportsOctober 31, 2023S. P. Mattiello, V. C. Barth, J. Scaria, C. A. S. Ferreira, S. D. Oliveira(dT23VN) (New England BioLabs, S1330S) and ProtoScript II Reverse Transcriptase (New England BioLabs, M0368L), followed by synthesis of the second-strand cDNA (dsDNA) using the NEBNext Ultra II Non-directional

(78) Transcriptome-wide analysis of the function of Ded1 in translation preinitiation complex assembly in a reconstituted in vitro systembioRxivOctober 20, 2023Fujun Zhou, Julie M. Bocetti, Meizhen Hou, Daoming Qin, Alan G. Hinnebusch, Jon R. Lorschpurified for reverse transcription using RT primer NI-802 and ProtoScript II Reverse Transcriptase (NEB; M0368S). The ~105 nt cDNAs were separated from free RT primers on a 15% TBE-Urea gel and circularized using

(79) The impact of Parkinson’s disease-associated gut microbiota on the transcriptome in DrosophilaMicrobiology SpectrumOctober 17, 2023Xin Liu, Meng Yang, Runzhou Liu, Fan Zhou, Haibing Zhu, Xiaoyun Wangthe fragmented and primed mRNA was reversed into cDNA using ProtoScript II Reverse Transcriptase (#M0368S, NEB) in a 20 µL reaction with the procedure of 10 minutes at 25℃ and 15 minutes at 42℃ followed by

(80) Efficient genetic editing of human intestinal organoids using ribonucleoprotein-based CRISPRDisease Models & MechanismsOctober 1, 2023Nefeli Skoufou-Papoutsaki, Sam Adler, Paula D'Santos, Liz Mannion, Shenay Mehmed, Richard Kemp, Amy Smith, Francesca Perrone, Komal Nayak, Alasdair Russell, et al.P-value). qPCR validation cDNA conversion was performed using ProtoScript II RT standard protocol (NEB, M0368) and Random Primer Mix (NEB, S1330S). qPCR was performed using TaqMan Fast Universal PCR Master Mix

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ProtoScript II Reverse Transcriptase

ProtoScript II Reverse Transcriptase from New England Biolabs

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ProtoScript II Reverse Transcriptase from New England Biolabs

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