ProtoScript II Reverse Transcriptase M0368S from New England Biolabs

Product Specs
ItemProtoScript II Reverse Transcriptase
CompanyNew England Biolabs
Price $99.00 Inquire
Catalog NumberM0368S
Size4000 units
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(41) Phosphorylation of endothelial histone H3.3 serine 31 by PKN1 links flow-induced signaling to proatherogenic gene expressionNature Cardiovascular ResearchJanuary 8, 2025Young-June Jin, Guozheng Liang, Rui Li, ShengPeng Wang, Mohamad Wessam Alnouri, Mette Bentsen, Carsten Kuenne, Stefan Günther, Yang Yan, Yongxin Li, et al.transcription was performed using the ProtoScript II Reverse Transcription Kit (New England Biolabs, M0368S). In brief, to synthesize cDNA, 1 µg of total RNA was combined with 0.5 µg of random hexamer primers

(42) Mapping the Genetic Architecture of the Adaptive Integrated Stress Response in S. cerevisiaebioRxivDecember 22, 2024Rachel Baum, Jinyoung Kim, Ryan Y. Muller, Nicholas T. Ingoliatranscription was carried out using 4 μg of RNA template with the ProtoScript II reverse transcriptase (NEB #M0368L) and 0.25 μM gene-specific priming (P(UBC6)-mScarlet: NI-1267; P(PCL5)-citrine: NI-1268) according to

(43) The SV40 virus enhancer functions as a somatic hypermutation-targeting element with potential tumorigenic activityTumour Virus ResDecember 1, 2024Šenigl F, Soikkeli AI, Prost S, Schatz DG, Slavková M, Hejnar J, Alinikula J.performed. The prepared RNA was reversely transcribed with Protoscript II Reverse Transcriptase (NEB, M0368) using random primers from the kit. An amount of 2 μL of cDNA was then used for quantitative PCR with

(44) Uncovering the transcriptome-wide RNA modifications in Acinetobacter baumanniiMicrobial GenomicsNovember 20, 2024Kah Ern Ten, Sadequr Rahman, Hock Siew Tansynthesized using oligo d(T)23VN and reverse transcribed using ProtoScript® II Reverse Transcriptase (NEB, M0368S). The RNA template was removed by RNase H (NEB, M0297S), and LongAmp® Taq DNA Polymerase amplified the

(45) KDM5C is a sex-biased brake against germline gene expression programs in somatic lineagesbioRxivNovember 13, 2024Katherine M. Bonefas, Ilakkiya Venkatachalam, Shigeki Iwasereverse transcribed using the ProtoScript II Reverse transcriptase kit from New England Biolabs (NEB #M0368S) and primed with oligo dT. Expression of Kdm5c was detected using the primers 5’-CCCATGGAGGCCAGAGAATAAG-3’

(46) G3BP isoforms differentially affect stress granule assembly and gene expression during cellular stressMolecular Biology of the CellNovember 1, 2024José M. Liboy-Lugo, Carla A. Espinoza, Jessica Sheu-Gruttadauria, Jesslyn E. Park, Albert Xu, Ziad Jowhar, Angela L. Gao, José A. Carmona-Negrón, Torsten Wittmann, Natalia Jura, et al.T4 RNA ligase 2 truncated KQ (NEB, M0373L). Linker-ligated RNA was reverse transcribed with Protoscript II (NEB, M0368L) for 30 min at 50°C. Template RNA was hydrolyzed with 1M NaOH for 20 min at 70°C. Gel slices of reverse

(47) CRISPRi with barcoded expression reporters dissects regulatory networks in human cellsbioRxivSeptember 11, 2024Jinyoung Kim, Ryan Y. Muller, Eliana R. Bondra, Nicholas T. Ingoliasynthesize cDNA, 1 μg total RNA was reverse transcribed using dT priming with Protoscript II (NEB, M0368L). qPCR was conducted using DyNAmo qPCR mastermix (ThermoFisher, F410L) according to manufacturer protocols

(48) Precise measurement of molecular phenotypes with barcode-based CRISPRi systemsbioRxivSeptember 10, 2024Joseph H. Lobel, Nicholas T. Ingoliawas used as input for cDNA synthesis using dT priming with Protoscript II Reverse transcriptase (NEB, M0368S). cDNA was then further diluted 2-fold in water and used as input for qPCR as described above. All qPCR

(49) BMP4-Induced Suppression of Breast Cancer Metastasis Is Associated with Inhibition of Cholesterol BiosynthesisInternational Journal of Molecular SciencesAugust 23, 2024Lap Hing Chi, Andrew D. Redfern, Terry C. C. Lim Kam Sian, Ian P. Street, Allan D. Burrows, Suraya Roslan, Roger J. Daly, Robin L. Andersonsynthesised using the ProtoScript II reverse transcriptase (New England Biolabs, Ipswich, MA, USA, #M0368) with random pentadecamers. Gene expression was determined using the SYBR green real-time PCR master

(50) Development of a highly sensitive platform for protein-protein interaction detection and regulation of T cell functionProceedings of the National Academy of Sciences of the United States of AmericaAugust 13, 2024Hayashi H, Mak TW, Tanaka Y, Kubo Y, Izumida M, Urae R, Matsuyama T.conducted for 60 min at 42 °C from 1 μg of total RNA using ProtoScript II Reverse Transcriptase (NEB # M0368L) and subjected to PCR using the specific primers (for anti-CD3ε-scFv-TM) listed in SI Appendix, Table

(51) Molecular consequences of acute versus chronic CDK12 loss in prostate carcinoma nominates distinct therapeutic strategiesbioRxiv : the preprint server for biologyJuly 19, 2024Frank S, Persse T, Coleman I, Bankhead A 3rd, Li D, De-Sarkar N, Wilson D, Rudoy D, Vashisth M, Galipeau P, et al.and quantified by Qubit (Thermo Q10211). Reverse transcription was performed with ProtoScript II (NEB M0368) using 4μM poly-dT plus 1μM random hexamer primers. qPCR was performed in 384-well plates (15μL reactions)

(52) Translational regulation enhances distinction of cell types in the nervous systemeLifeJuly 16, 2024Toshiharu Ichinose, Shu Kondo, Mai Kanno, Yuichi Shichino, Mari Mito, Shintaro Iwasaki, Hiromu TanimotoThermo Fisher Scientific). The rRNA-depleted samples were reverse-transcribed with ProtoScript II (M0368L, New England Biolabs), circularized with CircLigase II (CL9025K, Epicentre), and PCR-amplified using

(53) Human promoter directionality is determined by transcriptional initiation and the opposing activities of INTS11 and CDK9eLifeJuly 8, 2024Joshua D Eaton, Jessica Board, Lee Davidson, Chris Estell, Steven Westfor Illumina,NEB,Cat# E7765, Commercial assay or kit,Protoscript II reverse transcriptase,NEB,Cat# M0368, Commercial assay or kit,LUNA qPCR reagent,NEB,Cat# M3003, Commercial assay or kit,Ampure XP beads,Beckman Coulter

(54) Sse1, Hsp110 chaperone of yeast, controls the cellular fate during endoplasmic reticulum stressG3: Genes, Genomes, GeneticsJune 5, 2024Mainak Pratim Jha, Vignesh Kumar, Asmita Ghosh, Koyeli Mapa(NEB Cat No. B0368S), 2 µl of 0.1 M DTT (NEB Cat No. B1034A), 1 µl of ProtoScipt II RT (NEB Cat No. M0368S), 0.2 µl of RNase inhibitor (Roche Diagnostics Gmbh Cat No. 03335399001), and 2.8 µl of nuclease-free

(55) Loss of tumor-derived SMAD4 enhances primary tumor growth but not metastasis following BMP4 signallingCell Communication and SignalingApril 30, 2024Lap Hing Chi, Andrew D. Redfern, Suraya Roslan, Ian P. Street, Allan D. Burrows, Robin L. AndersonSingle strand cDNA was synthesized using the ProtoScript II reverse transcriptase (New England Biolabs #M0368) with random pentadecamers. Gene expression was determined using the SYBR green real-time PCR master

(56) Transcriptome-wide analysis of the function of Ded1 in translation preinitiation complex assembly in a reconstituted in vitro systemeLifeApril 4, 2024Fujun Zhou, Julie M Bocetti, Meizhen Hou, Daoming Qin, Alan G Hinnebusch, Jon R Lorschpurified for reverse transcription using RT primer NI-802 and ProtoScript II Reverse Transcriptase (NEB; M0368S). The ~105 nt cDNAs were separated from free RT primers on a 15% TBE-Urea gel and circularized using

(57) The CARD8 inflammasome dictates HIV/SIV pathogenesis and disease progressionCellFebruary 29, 2024Wang Q, Clark KM, Tiwari R, Raju N, Tharp GK, Rogers J, Harris RA, Raveendran M, Bosinger SE, Burdo TH, et al.,Thermo Fisher Scientific,K1032 Recombinant Cas9 protein,IDT,1081059 ProtoScript II Reverse Transcriptase,NEB,M0368 IPTG,Sigma,I6758 Lenti-X Concentrator,TaKaRa,631232 Critical commercial assays,Critical commercial

(58) Protein-protein interactions with G3BPs drive stress granule condensation and gene expression changes under cellular stressbioRxivFebruary 10, 2024José M. Liboy-Lugo, Carla A. Espinoza, Jessica Sheu-Gruttadauria, Jesslyn E. Park, Albert Xu, Ziad Jowhar, Angela L. Gao, José A. Carmona-Negrón, Torsten Wittmann, Natalia Jura, et al.T4 RNA ligase 2 truncated KQ (NEB, M0373L). Linker-ligated RNA was reverse transcribed with Protoscript II (NEB, M0368L) for 30 minutes at 50°C. Template RNA was hydrolyzed with 1M NaOH for 20 minutes at 70°C. Gel slices

(59) Defining the mechanisms and properties of post-transcriptional regulatory disordered regions by high-throughput functional profilingbioRxivFebruary 8, 2024Joseph H. Lobel, Nicholas T. Ingoliaspectrophotometry. Reverse transcription was performed with dT priming using the ProtoScript II RT (NEB M0368L) as described by the manufacturer, scaled up two-fold. RNA was the digested by adding 0.5 μL each of

(60) Massive contractions of myotonic dystrophy type 2-associated CCTG tetranucleotide repeats occur via double-strand break repair with distinct requirements for DNA helicasesG3: Genes, Genomes, GeneticsFebruary 7, 2024David Papp, Luis A Hernandez, Theresa A Mai, Terrance J Haanen, Meghan A O’Donnell, Ariel T Duran, Sophia M Hernandez, Jenni E Narvanto, Berenice Arguello, Marvin O Onwukwe, et al.(cDNA) was synthesized using 0.5-μg total RNA as template, ProtoScript II Reverse Transcriptase (NEB M0368), and equal amounts of random hexamer primers (Thermo Fisher SO142) and oligo(dT) primers (Thermo Fisher

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ProtoScript II Reverse Transcriptase

ProtoScript II Reverse Transcriptase from New England Biolabs

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ProtoScript II Reverse Transcriptase from New England Biolabs

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