Human LGR-5, hFc Tag captured on CM5 Chip via Protein A can bind Human R-Spondin 3, His Tag with an affinity constant of 0.59 nM as determined in SPR assay (Biacore T200) (Routine Test).
Immobilized Human R-Spondin 3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 25.2ng/ml determined by ELISA (QC Test).
Immobilized Human R-Spondin 3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 8.8ng/ml determined by ELISA (QC Test).
Human R spondin 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 1.0 - 10.0 ng/mL in the presence of 5 ng/mL recombinant Human Wnt Surrogate-Fc Fusion (WNT-HM23A).
Supplier Page from Kactusbio Inc. for Human R spondin 3/RSPO3 Protein (RSP-HM103)