EGF Protein 236-EG-200 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemEGF Protein
CompanyR&D Systems, a Bio-Techne Brand
Price $152.00Supplier Page View Company Product Page
Catalog Number236-EG-200
SpeciesHuman
Source/Expression SystemE. coli-derived Recombinant Human EGF Protein, CF
FormatLyophilized from a 0.2 µm filtered solution in PBS
Size200 ug (also 1 mg)
Molecule NameEGF
Purity>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
Molecular WeightObserved: 6 kDa, reducing conditions
Theoretical: 6 kDa
ApplicationsBioactivity
Conjugate/TagUnconjugated
NCBI Full Gene Nameepidermal growth factor
NCBI Gene AliasesURG, HOMG4
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Sizes Available: 200 ug (also 1 mg)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Ferroptosis and Wnt/β-Catenin Signaling Triggered by Environmentally Relevant Nanoscale Polypropylene Plastics in Human Intestinal ModelsACS Environmental AuMay 20, 2026Sadman Sakib, Maohui Chen, Stephanie Skerrett, Daniel Prezgot, Magda Vandenberg, Adrian F. Pegoraro, Zygmunt J. Jakubek, Zeina Maan, Shan Zoufetal bovine serum (FBS) (A5209502, ThermoFisher Sci.) and 10 ng/mL of Epidermal Growth Factor (EGF, 236-EG-200, R&D Systems). Caco-2 (HTB-37, ATCC) cells were cultured in Eagle’s Minimum Essential Medium (30–2003,

(2) Human pancreatic progenitor organoids define genetic and epigenetic barriers to early PDAC transformationDev CellMay 19, 2026Xian Zhang, Wilfred Wong, Hyein S. Cho, Dapeng Yang, Gary Dixon, Renhe Luo, Dingyu Liu, Denis Torre, Shigeaki Umeda, Federico González, et al.N0636–100G), 0.5μM A83–01 (Tocris, 2939), 100ng/ml hNoggin (Peprotech, 120–10c), 50ng/ml hEGF (R&D Systems, 236-EG-200), 10nM hGastrin I (Sigma-Aldrich, G9020–250UG), 100ng/ml hFGF10 (PeproTech,100-26-500UG), 500ng/ml Rsp1

(3) Valosin-Containing Protein Contributes to Plexiform Neurofibroma Formation and Represents a Novel Therapeutic TargetCellsMay 6, 2026Lalitha Gopalan, Youjin Na, Liang Hu, Ashley Hall, Mi-Ok Kim, Eva Dombi, Sara Szabo, Nancy Ratner, Gang Huang, Jianqiang Wuwith 1 mL of medium containing DMEM:F-12 (3:1), 20 ng/mL of rhEGF (R&D Systems, Minneapolis, MN, USA; #236-EG-200), 20 ng/mL rh bFGF (PeproTech, Cranbury, NJ, USA; #450-33-50 µg), 1% B-27 supplement (Life Technologies,

(4) Transcriptomic and phenotypic convergence of neurodevelopmental disorder risk genes in vitro and in vivoNature NeuroscienceApril 24, 2026Meilin Fernandez Garcia, Kayla Retallick-Townsley, April Pruitt, Elizabeth A. Davidson, Novin Balafkan, Jonathan Warrell, Tzu-Chieh Huang, Alfred Kibowen, Zhiyuan Chu, Yi Dai, et al.(1:100; Life Technologies, cat. no. 17502048), recombinant human EGF (10 ng ml−1; R&D Systems, cat. no. 236-EG-200), recombinant human basic FGF (10 ng ml−1; Life Technologies, cat. no. 13256029), Geneticin (0.5 mg

(5) A degrader of HER2 and EGFR abolishes p95HER2 and shows robust antitumor efficacy in HER2-positive breast cancerSci RepApril 20, 2026Lu Yang, Arup Bhattacharya, Yun Li, Darrell Peterson, Yuesheng ZhangHydrocortisone (Cat# H0888), and cholera toxin (Cat # C8052) were purchased from Sigma-Aldrich. EGF (Cat # 236-EG-200) was purchased from R&D Systems. Insulin (Cat # 12,585,014) was purchased from Thermo Fisher Scientific. Western blot analysis

(6) Intracellular enzymatic reducing systems control receptor tyrosine kinase signaling via PTP1BSci AdvApril 10, 2026Lucia Coppo, Wenchao Zhao, Qing Cheng, Axel Tobias Scholz, Elias S. J. Arnér, Markus Dagnelland streptomycin. No “additional selenium source” was added. EGF ligand (100 ng/ml; R&D Systems, no. 236-EG-200) stimulations, at indicated time points, were performed in overnight-starved (0.1% FBS) cells transiently

(7) Targeting Tumour Microtubes to Disrupt Glioma NetworksResearch SquareApril 7, 2026Tengfei Huang, Tengfei Huang, Suchet Zhang, Suchet Taori, Donghai Wang, Donghai Wang, Huairui Chen, Huairui Yuan, Tingting Duan, Tingting Duan, et al.without vitamin A (Gibco, Cat# 12587010), 20 ng/ml epidermal growth factor (EGF) (R&D Systems, Cat# 236-EG-200), 20 ng/ml recombinant human basic fibroblast growth factor (bFGF) (R&D Systems, Cat# 233-FB-025), 1

(8) Do drugs with biliary toxicity cause cholangiocarcinoma?bioRxivApril 3, 2026Cai Zong, Kyumin Lim, Sierra A. Walker, Rui Dai, Mi Ho Jeong, Reanna George, Jung Hyun Jo, Shams Iqbal, Hyungsoon Im, Ralph Weissleder620 ng/ml hydrocortisone (Cat. H0888, Sigma-Aldrich) and 10 ng/ml epidermal growth factor (EGF; Cat. 236-EG-200, R&D Systems) at 37 °C in 5% CO2. The culture medium was freshly prepared and used within one month.

(9) TPP-45142—an Anti-HER2 T-cell Engager—Designed for Selective HER2-Low Cancer ImmunotherapyMolecular Cancer TherapeuticsApril 2, 2026Evelyn De Tavernier, Peter S. Kim, Eduardo M. Bruch, Virna F. Cortez-Retamozo, Lien Timmerman, Alyssa L. Flynn, Wouter Van Overbeke, Fabrice Tirode, Valeria Cintra Barbosa-Lorenzi, Peter Piepenhagen, et al.Six 1/10 serially diluted curves were applied, starting at 100 nmol/L. Human EGF (R&D Systems, cat. #236-EG-200) was used as a positive control. At least four zero-dose (0 nmol/L compound) wells were included in

(10) HAND1 controls the lineage bifurcation of trophoblast and amnion from human pluripotent stem cellsCell Mol Life SciMarch 10, 2026Changmiao Pang, Qifeng Yang, Yulong Zhong, Jinhao Ye, Yufang Lv, Shufei Xie, Yanqing Tang, Xianhua Ye, Feifan Zhang, Chao Li, et al.A83-01(TOCRIS, 2939), 10 μM valproic acid sodium salt (APExBIO, Valproic acid), 50 ng/ml EGF (R&D, 236-EG-200), 5 μM Y-27632 (APExBIO, A3008), 0.1 mM 2-mercaptoethanol (Sigma, M6250)]. TSC-like colonies were picked

(11) Discovery of BBO-11818, a Potent and Selective Noncovalent Inhibitor of (ON) and (OFF) KRAS with Activity against Multiple Oncogenic MutantsCancer DiscovMarch 6, 2026Carlos Stahlhut, Anna E. Maciag, Kyle A. Sullivan, Kanchan Singh, Nadege Gitego, Zuhui Zhang, Albert H. Chan, Alok K. Sharma, Patrick A. Alexander, Jin Shu, et al.Selleckchem and stored at 4°C. Recombinant human EGF protein was purchased from Bio-Techne R&D Systems (#236-EG-200), dissolved in PBS, aliquoted, and stored at −80°C. PPI Assay for RAS–RAF Disruption Avi-KRASWT or

(12) Development, Expansion, and Histological Characterization of Patient-Derived Liver Organoids for Drug Screening and Disease ModelingBio ProtocMarch 5, 2026Silvia De Siervi, Stefania Mantovani, Barbara Oliviero, Mario U. Mondelli, Martina Di Noia, Cristiana Soldani, Barbara Franceschini, Fabrizio De Luca, Marcello Maestri, Salvatore Corallo, et al.number: ECM0728) 8. Epidermal growth factor (EGF), recombinant human (R&D Systems, catalog number: 236-EG-200) 9. Fetal bovine serum (FBS) (Gibco, catalog number: 10270-106) 10. FGF-19, recombinant human (Peprotech,

(13) Development, Expansion, and Histological Characterization of Patient-Derived Liver Organoids for Drug Screening and Disease ModelingBIO-PROTOCOLMarch 5, 2026Silvia De Siervi, Stefania Mantovani, Barbara Oliviero, Mario U. Mondelli, Martina Di Noia, Cristiana Soldani, Barbara Franceschini, Fabrizio De Luca, Marcello Maestri, Salvatore Corallo, et al.number: ECM0728) 8. Epidermal growth factor (EGF), recombinant human (R&D Systems, catalog number: 236-EG-200) 9. Fetal bovine serum (FBS) (Gibco, catalog number: 10270-106) 10. FGF-19, recombinant human (Peprotech,

(14) Shape-conformal porous frameworks for full coverage of neural organoids and high-resolution electrophysiologyNature Biomedical EngineeringFebruary 18, 2026Naijia Liu, Shahrzad Shiravi, Tianqi Jin, Jiaqi Liu, Zhengguang Zhu, Jiying Li, Ingrid Cheung, Haohui Zhang, Yue Wang, Qingyuan Li, et al.μM, MTocris, 4423409), retinoic acid (0.1 μM; Sigma-Aldrich, R2625) and EGF (20 ng ml−1; R&D Systems, 236-EG-200) as growth factors for neural induction. From day 19, the medium was enriched with BDNF (20 ng ml−1,

(15) Human lung organoids model for assessing host response to Mycobacterium tuberculosis infectionFrontiers in Cellular and Infection MicrobiologyFebruary 10, 2026Chaofan Li, Pengfei Zhong, Zhimin Yun, Leiming Fang, Meida Xiang, Qisheng Su, Jiru Wang, Hebing Chen, Zhi Chen, Liang Yue, et al.251-KG-050), 100 ng/mL FGF10 (R&D Systems, 345-FG-250), 3 μM CHIR99021 and 50 ng/mL EGF (R&D Systems, 236-EG-200) was added [supplemented with BSA (Sigma-Aldrich, V900933), 1× N2 (Gibco, 17502048), 1× B27 (Gibco,

(16) Generation of xenobiotic free retinofugal assembloidsFrontiers in Cell and Developmental BiologyJanuary 14, 2026Michael H. Hayes, Maria F. Valdes Michel, Markus H. Kuehn, Randy H. Kardon, Oliver W. Gramlich50 mL aliquots with: o 20 ng/mL FGF-2 (R&D Systems, Cat# 3718-FB) o 10 ng/mL EGF (R&D Systems, Cat# 236-EG-200) o 1% recombinant human extracellular matrix (ECM) mix o 20 ng/mL BDNF (R&D Systems) o 20 ng/mL NT-3

(17) Characterization of a pathogenic subpopulation of human glioma associated macrophages linked to glioma progressionCancer CellJanuary 12, 2026Kenny Kwok Hei Hei Yu, Zaki Abou-Mrad, Kristof Törkenczy, Isabell Schulze, Jennifer Gantchev, Gerard Baquer, Kelsey Hopland, Evan D. Bander, Umberto Tosi, Cameron Brennan, et al.Supplement,GIBCO/ThermoFisher,cat# 17502048 Recombinant Human Epidermal Growth Factor,R&D Systems,cat# 236-EG-200 Recombinant Human FGF basic/FGF2/bFGF,R&D Systems,cat# 233-FB-500 Poly-L-Ornithine hydrobromide,Sigma-Aldrich,cat#

(18) TPP-45142—an Anti-HER2 T-cell Engager—Designed for Selective HER2-Low Cancer ImmunotherapyMol Cancer TherJanuary 8, 2026Evelyn De Tavernier, Peter S. Kim, Eduardo M. Bruch, Virna F. Cortez-Retamozo, Lien Timmerman, Alyssa L. Flynn, Wouter Van Overbeke, Fabrice Tirode, Valeria Cintra Barbosa-Lorenzi, Peter Piepenhagen, et al.Six 1/10 serially diluted curves were applied, starting at 100 nmol/L. Human EGF (R&D Systems, cat. #236-EG-200) was used as a positive control. At least four zero-dose (0 nmol/L compound) wells were included in

(19) Impaired Complex I dysregulates neural/glial precursors and corpus callosum development revealing postnatal defects in Leigh syndrome miceEMBO Molecular MedicineDecember 22, 2025Sahitya Ranjan Biswas, Porter L Tomsick, Colin Kelly, Brooke A Lester, Julia P Milner, Sara N Henry, Yairis Soto, Samantha Brindley, Nicole DeFoor, Paul D Morton, et al.(Gibco) supplemented with N2 supplement (Fisher Scientific #17502048), EGF (20 ng/μL, R&D Systems # 236-EG-200), and FGF (20 ng/μL, R&D Systems # 233-FB-025/CF) from P14 animals. Cells were plated in ultra-low adherent

(20) A human iPSC-based neural spheroid platform for modelling glioblastoma infiltration using high-content imagingScientific ReportsDecember 13, 2025Victoria S. K. Tsang, Federica Riccio, Aimee S. Wilson, Hannah Nudds, Jason D. Coombes, Heiko Wurdak, Harry J. C. J. Bulstrode, Ivo Lieberam, Davide Danovi(Invitrogen 17504044), 0.5X N2 (Invitrogen 17502048), 40 ng/mL epidermal growth factor (EGF) (R&D Systems 236-EG-200), and 40 ng/mL basic-fibroblast growth factor (b-FGF) (Invitrogen PHG0026). Cells were passaged using

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1/13/2023EGF (236-EG) Protein Works Well Liyong Z.To induce EMT for BT20 cells, 20 ng/ml EGF was administered for incubation of 72 h. The cells were collected for fractionation using NE-PER Nuclear and Cytoplasmic Extraction Reagents (78833). The ... Read Review

Recombinant Human EGF Protein, CF

EGF Protein

EGF Protein

EGF Protein

EGF Protein

EGF Protein from R&D Systems, a Bio-Techne Brand

Description

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (1036)

Reviews

EGF Protein from R&D Systems, a Bio-Techne Brand

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